Pseudomonas sp. CB-33이 생산하는 β-Xylosidase의 특성

유진환,김현구,김치경,임재윤 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

Pseudomonas sp. CB-33 균주의 배양 상등액으로부터 ammonium sulfate 침전법, PEI 침전법, DEAE-Sephadex column chromatography, gel column chromatography 그리고 preparative disc gel electrophoresis를 이용하여 β-xylosidase를 정제하였다. 정제된 효소의 Km 값은 4.6 mM이었다. 효소활성의 최적 pH는 7.0이었으며 pH 6.5~9 범위에서 안정하였다. 최적 활성온도는 45℃였으며, 각 온도에서 30분 동안 정치하였을 때 35℃까지 안정하였다. SDS-PAGE에 의해 효소의 분자량을 측정해 본 결과 약 44,000dalton이었다. HG^2+, Cu^2+ 그리고 Zn^2+는 효소활성을 저해하였고, 여러 화학변형제로 효소에 처리해 본 결과 tyrosine과 tryptophan이 효소활성에 관여하고 있을 것으로 추정된다. 여러가지 기질에 대한 특이성을 조사해 본 결과 pNPX 뿐만 아니라 pNPA도 분해하였다. Xylan에 endoxylanase만을 작용시켰을 때보다 β-xylosidase와 endoxylanase를 혼합작용 시켰을 때 xylan의 가수분해도는 약 2배 정도 증가하였다. 그리고 Pseudomonas sp. CB-33 균주가 생산하는 xylan 분해효소는 oat spelt xylan보다 birchwood xylan에 보다 효과적으로 작용하였다. The β-xylosidase was purified 99-fold from the culture supernatant of Pseudomonas sp. CB-33 by ammonium sulfate precipitation, PEI precipitation, DEAE-Sephadex column chromatography, Sephadex G-75 gel filtration chromatography and preparative disc gel electrophoresis. Molecular weight of the enzyme was estimated to be 44,000 by SDS polyacrylamide gel electrophoresis. The enzyme has a pH optimum for activity at 7.0 and is stable over pH 6.5~9.0. The optimal temperature of the enzyme was 45℃, and it enzymatic activity was completely inactivated at 55℃ for 30 min. Km value of the enzyme for p-nitrophenyl- β-D-xylopyranoside was calculated to be 4.6 mM. The effect of various reagents on the β-xylosidase activity was investigated. The enzyme activity was completely inhibited by Hg^2+, Cu^2+, and Zn^2+. The β-xylosidase was inactivated by tryptophan-specific reagent, N-bromosuccinimide and the enzyme was competitively inhibited by xylose. The β-xylosidase and endoxylanase from Pseudomonas sp. CB-33 hydrolized xylan synergically. The purified enzyme also showed α-L-arabinofuranosidase activity.

성패귀인의 성차에 대한 연구

정진경, 류윤상, 최현주 忠北大學校 學生生活硏究所 1994 學生生活硏究 Vol.19 No.1

본문참고

폐흡충증으로 확진된 고립성 폐결절 1예

유영권,김규식,김윤현,나국주,김경수,임성철,김영철,박경옥 全南大醫大 2001 전남의대학술지 Vol.37 No.2

남성유방에서 발생한 침윤성 선암종 1 예 : Report of a case

정유경,문현준,전호종 조선대학교 1995 The Medical Journal of Chosun University Vol.20 No.1

Male breast cancer is rare compared to female breast cancer, about 1% or all breast cane or. The cause of male breast cancer is as poorly understood, but several etiologic factors have been suggested in the literature. Proposed risk factors include exogenous estrogen exposure, Klinefelter’s syndrome, gynecomastia and familial clustering. All of the microscopic types identified in the female breast have been encountered in males : the most common type is infiltrating duct carcinoma. Most often, it presents as a painless lump and subareolar in origin. Comparison with female breast cancer reveals no difference with regard to incidence of positive axillary lymph nodes. However there is a sightly lower survival rate for men. The stage of disease is the only parameter that significantly affects outcome. We report a case of breast cancer arising right subareolar area in a 57 year-old male. There were no familial history, Klinefelter's syndrome and microscopic evidence of gynecomastia. Histologically, this tumor was infiltrating duct carcinoma disposed in nests sometimes glands .

peat moss를 이용한 폐수중의 중금속 제거

이경화,김정섭,유현철,최성우,윤철종,우성훈,박승조 동아대학교 환경문제연구소 2001 硏究報告 Vol.24 No.1

Adsorption equilibrium of heavy metals ion on the peat moss was confirmed Frundlich isotherm Removal efficiency of heavy metals ion was increased from pH 2 to 10 and heavy metal removal efficiency was increased in the sequence of Cu(Ⅱ) > Pb(Ⅱ) > Cd(Ⅱ) > Mn(Ⅱ) on the PM(Peat Moss). The PM is likely to be more effective adsorption than the PAC to removal of metal ions in the solution, since PM's weakly acidic functional groups which is not founded on the PAC, in addition to its high surface area and porosity. But, the PM is not separated from the solution water by sedimentation process. So, a liquid and solid seperation between the PM and the solution water could be considered for the PM's field application. But peat moss was low cost adsorbent. Thus author propose to adopt PM as a adsorbent for heavy metal removal.

부산 일부 대학교 학생들의 니코틴 의존도와 구취의 연관성

김유린(외 5명) 신라대학교 자연과학연구소 2012 自然科學論文集 Vol.22 No.-

The purpose of this study is to examine the association of bad breath and nicotine dependency. By use of the findings from this study, This study conducted the survey of 53 smoker who visited dental hygiene lab for delivery from October to November 2012 with structured questionnaires(Fagerstrom Tolerance Questionnaire FTQ) and equipment(CO measuring instrument, Oral Chroma etc). The data were analyzed by using SPSS 20.0 program for χ2-test, ANOVA. Smokers' average age was 23 years old. The more higher nicotine dependence the more the average age was higher. The more higher nicotine dependence the less to nque brushing. No association of nicotine dependence and self-bad breath level. CO State was Significant differences(ND1(M=2.58), ND2(M=3.98), ND3(M=7.41), ND4(M=4.19), ND5(M=13.14))(F=2.616, p=.047). Dimethyl Sulfide was Significant differences(F=3.026, p=.026).

전류밀도 향상을 위한 금속 산화물 피막 제조에 관한 연구

채경선,유국현 동국대학교 자연과학연구원 2000 자연과학연구 논문집 Vol.5 No.-

구조물의 부식을 저감시키기 위한 재료로 사용되려면 전기 전도성이 좋아야 하며, 여러 산업환경에 적용될 수 있도록 내식성이 뛰어나야한다. 이리 듐-루테늄 산화물 피막은 이러한 성질을 만족시키는 물질로, 이들 산화물 피막 의 성질은 대개 양극 제조 공정에 의해 크게 좌우되므로 본 연구에서는 전류 밀도 향상을 위한 제조 공정을 최적화하고자 하였다. Dip-coating법으로 전도성 코팅층인 이리듐-루테늄 산화물 피막을 기지금속인 티타늄 위에 피복 시키는데, 이 코팅층의 내식성 향상을 위해서 다구치 방법을 도입하여 최적 공정 조건을 구하였다. 이때 사용된 변수는 전처리 방법, 열처리 온도, 열처리시 주입 가스량, 열처리 시간으로 이들 변수를 3가지로 변화시켜 실험하였다. 실험결과 이리듐-루테늄 산화물 피막 제조시 기판위에 티타늄산화물 중간층을 plasma spray법으로 제조하고, 열처리 온도 450℃, 주입가스량 3sccm, 열처리시간을 120분으로 하였을 때 방식전류밀도가 가장 우수한 코팅층(활성화 코팅층)을 얻었다.

다중회귀모형을 이용한 수완부 골성숙도의 추정에 관한 연구

김경호,유형석,김석현 대한치과교정학회 1997 대한치과교정학회지 Vol.27 No.5

성장의 잠재력에 대한 평가는 개체의 성숙도를 나타내는 여러 생리학적 지표(physiologic indicators)들에 의해 이루어지며 그 중 골성숙도(skeletal maturity)는 성적성숙도(sexual maturity)와 신장의 성장변화와 밀접한 관계가 있는 것으로 알려져 있으나, 치아발육과의 상관관계에 대해선 논란이 많은 실정이다. 그러나 최근의 연구에 의하면 하악견치를 포함한 일부치아의 발육이 골성숙도와 밀접한 관련이 있는 것으로 알려져 있으나, 치아발육과의 상관관계에 대해선 논란이 많은 실정이다. 그러나 최근의 연구에 의하면 하악 견치를 포함한 일부 치아의 발육이 골성숙도와 밀접한 관련이 있는 것으로 보고된 바 있다. 이에, 본 연구에서는 7세에서 15세까지의 한국인 남, 녀 아동 387명의 수완부 방사선 사진과 파노라마 사진을 이용하여 Fishman방법과 Greulich와 Pyle방법에 의해 골성숙도을 평가하였으며 변형된 Demirjiran방법에 의해 치아의 성숙도를 평가하여, 수완부 방사선의 사진의 도움 없이 골성숙도를 추정할수 있는 방법을 구하고자 다중회귀모형을 이용한 수완부 골성숙도의 추정에 관한 연구를 시행하였으며 다음과 같은 결론을 얻었다. 1. 골성숙 지수의 추정을 위한 아래의 다중회귀모형은 84%의 설명력을 나타내며 연대연령, 성별, 하악 견치의 회귀계수는 통계학적 유의성을 보였다. 골성숙지수 = 0.60x 연대연령 -1.67x성별 + 0.88x하악견치 - 0.05x하악 제2대구치 -10.3 2. 골연령의 추정을 위한 아래의 다중회귀모형은 87%의 설명력을 나타내며 연대연령, 성별, 하악 견치의 회귀계수는 통계학적 유의성을 보였다. 골연령 = 0.75x 연대연령 - 0.55x성별 + 0.71x하악 견치 + 0.09x하악 제2대구치- 5.77 (성별: 남자=1, 여자=0., 하악견치, 제2대구치 : 각 발육단계별 평균연령) The evaluation of growth potency can be done with many physiologic indicators. It has been well known that skeletal maturity has a close relation with both sexual maturity somatic maturity, but the correlation between skeletal maturity and dental maturity was believed to be less certain. But, recent studies show that specific teeth, including lower canines, present close correlations with skeletal maturity. So, in this study, we study, we studied hand-wrist X-ray films and orthopantomograms of 387 Korean boys and girls aged from 7 to 15; the purpose was to determine skeletal and dental maturity, and to find out a new method to estimate individual skeletal maturity using multiple-regression model, without the help of hand-wrist X-ray film. As a result of this study, followings were observed. 1. The following multiple-regression model can estimate skeletal maturity index(SMI) with 84% of accuracy, and regression coefficient of chronologic age, sex and lower canine show statistical significance. SMI = 0.60 x chronologic age - 1.67 x sex** + 0.88 x lower canine* - 0.05 x lower 2nd molar* - 10.3 *: mean age corresponding each developing atage, **: male= 1, femal = 0 2. The following multiple-regression model can estimate skeletal age with 87% of accuracy, and regression coefficient of chronologic age, sex and lower canine show statistical significance. Skeletal age = 0.75 x chronologic age - 0.55 x sex** + 0.71 x lower canine* - 0.09 x lower 2nd molar* - 5.77 *: mean age corresponding each developing atage, **: male= 1, femal = 0

아메바성 대장염과 연관된 Henoch-Scho¨nlein Purpura 1예

장재현,원경준,이준,변유미,이재광,김영훈,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.3

Henoch-SchonIein purpura is a common form of vasculitis primarily involving the skin, the gastrointestinal tract, the joints, the kidneys, and the nervous system. The etiological investigations are essential, because triggering factors are found in approximately half the patients such as viral, bacterial and parasitic infections, drugs, toxins, systemic diseases and cancer. But it's the correlation between Henoch-SchonIein purpura and Entamoeba histolytica has been rarely reported. We report a 29-years-old woman with Henoch-SchoIein purpura complicated by the lower gastrointestinal bleeding caused by the amoebic colitis. She complained of cramping abdominal pain, lower extremity purpura and hematochezia. Cysts and trophozoites of Entamoeba histolytica were detected in her stool. Skin biopsy demonstrated the leukocytoclastic vasculitis. She was administered metronidazole 1,500㎎ a day for the treatment of amebic colitis. Her hematochezia was improved, but the abdominal pain and purpura were not. The subsequent corticosteroid therapy improved her symptoms.

다공성 펠렛을 포집재로 사용하는 매연여과장치의 배압 및 재생 특성에 관한 연구

金鎭玄,柳鍾植,文學勳,車京玉 明知大學校 産業技術硏究所 2006 産業技術硏究所論文集 Vol.25 No.-

Diesel particulate trap is a core technology for the reduction of PM from diesel vehicles. This study presents the features and the characteristics of DPF system when using pellet type filters. In comparison with wall-flow filter, the pellet filter has the advantages of cracking free during regeneration and shape flexibility. Experiments are conducted in a test bench simulated as diesel engine exhaust condition. Pressure drop and particle loading rate was compared by using two pellet filters having the porosity of 70% and 0%. Also its regeneration was tested.