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Papaverine이 저분극된 토끼 유두근의 수축력과 활동전압에 미치는 영향
임인자,조성일,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1991 中央醫大誌 Vol.16 No.3
In Partially deppolarized, isolated papillary muscle from rabbit heart, the effect of papaverine on contractile force and calcium mediated slow action potential was investigated. Results were as follows; 1. Papaverine induced the negative inotropic effect dose-dependently in the majority of preparations, although some preparations showed the opposite namely positive inotropic response. The significant correlation was found between the degree of depression in tension and increase that of in drug concentration. 2. The contractile force depressed by papaverine was restored by calcium(4 mM). 3. In partially depolarized papillary muscle, papaverine reduced slightly both action potential amplitude and upstroke velocity (dV/dt_max). The duration of action potential (duration of 90% repolarization) was also increased by papaverine in a dose-dependent manner. 4. The resting membrane potential was not altered in low concentrations (10^-7∼5X 10^-6M) of papaverine, however, it was significantly decreased in high concentration (10^-5∼5X 10^-5M) of the drug. The slightly increased overshoot potential was also observed in the above at high concentration range. From these results, it is suggested that in papillary muscle of rabbit heart, inhibitory effect of papaverine on the contractility may be attributable to its antagonistic action on the calcium current rather than its inhibitory effect on the phosphodiesterase activity. The local anesthetic property of the drug may also contribute to the papaverine induced decreases in both contractile force and upstroke velocity. The prolongation of action potential duration brought by papaverine appears to be induced not by increase in calcium currents but by a decrease in K^+ conductance.
조태웅,민영돈,김경종,정권율,강성인,류소연,임성철 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.1
Obturator hernia is a rare pelvic hernia, occuring most frequently in elderly, extra-thin or debilitated women. Because of the anatomic character of the obturator foramen and the nonspecific symptoms in the presence of the hernia, the risk of strangulation of the herniated bowel is high, which leads to high morbidity and mortality rate. The authors experienced an unusual case of left obturator hernia with strangulation of the small bowel in an 80-year old female. The hernia was diagnosed by abdomino-pelvic computed tomography (CT) scan. Through the lower midline transperitoneal approach, resecting the strangulated small bowel, the hernia sac was ligated and the obturator foramen was repaired using adjacent broad ligament. We report these findings with a brief review of the literature.
송근영,강길,박정희,김경종,민영돈,박치영,임성철 朝鮮大學校 附設 醫學硏究所 2002 The Medical Journal of Chosun University Vol.27 No.2
Gastric small cell carcinoma(SCC) is defined as poorly-differentiated neuroendocrine tumor of the stomach, and their morphologic and biologic features arc similar to those of the lung. It is known that this tumor has poor prognosis even in early pathologic stage, It acounts for only 3% of all gastrointestinal SCC. that has been reported only 33 cases outside and 6 cases inside of the comitiy. Most of these reports are related to advanced gastric SCC. as pathologic stage IIIb or IV. This report describes a case of early gastric SCC. pathologic stage Ib.
Lim, Sung Don,Hwang, Jin-Gyu,Jung, Chang Gyo,Hwang, Sun-Goo,Moon, Jun-Cheol,Jang, Cheol Seong Oxford University Press 2013 DNA research Vol.20 No.3
<P>A large number of really interesting new gene (RING) E3 ligases contribute to the post-translational modification of target proteins during plant responses to environmental stresses. However, the physical interactome of RING E3 ligases in rice remains largely unknown. Here, we evaluated the expression patterns of 47 <I>Oryza sativa</I> RING finger protein (<I>OsRFP</I>) genes in response to abiotic stresses via semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and <I>in silico</I> analysis. Subsequently, molecular dissection of nine <I>OsRFPs</I> was performed by the examination of their E3 ubiquitin ligase activity, subcellular localization, and physical interaction with target proteins. Most of the OsRFPs examined possessed E3 ligase activity and showed diverse subcellular localization. Yeast two-hybrid analysis was then employed to construct a physical interaction map of seven OsRFPs with their 120 interacting proteins. The results indicated that these OsRFPs required dynamic translocation and partitioning for their cellular activation. Heterogeneous overexpression of each of the <I>OsRFP</I> genes in <I>Arabidopsis</I> suggested that they have functionally diverse responses to abiotic stresses, which may have been acquired during evolution. This comprehensive study provides insights into the biological functions of OsRFPs, which may be useful in understanding how rice plants adapt to unfavourable environmental conditions.</P>
Lim, Sung Don,Cho, Hyun Yong,Park, Yong Chan,Ham, Deok Jae,Lee, Ju Kyong,Jang, Cheol Seong Oxford University Press 2013 Journal of experimental botany Vol.64 No.10
<P>Thermotolerance is very important for plant survival when plants are subjected to lethally high temperature. However, thus far little is known about the functions of RING E3 ligase in response to heat shock in plants. This study found that one rice gene encoding the RING finger protein was specifically induced by heat and cold stress treatments but not by salinity or dehydration and named it <I>OsHCI1</I> (<I>Oryza sativa</I> heat and cold induced 1). Subcellular localization results showed that OsHCI1 was mainly associated with the Golgi apparatus and moved rapidly and extensively along the cytoskeleton. In contrast, OsHCI1 may have accumulated in the nucleus under high temperatures. OsHCI1 physically interacted with nuclear substrate proteins including a basic helix-loop-helix transcription factor. Transient co-overexpression of OsHCI1 and each of three nuclear proteins showed that their fluorescent signals moved into the cytoplasm as punctuate formations. Heterogeneous overexpression of <I>OsHCI1</I> in <I>Arabidopsis</I> highly increased survival rate through acquired thermotolerance. It is proposed that OsHCI1 mediates nuclear–cytoplasmic trafficking of nuclear substrate proteins via monoubiquitination and drives an inactivation device for the nuclear proteins under heat shock.</P>
Sung Don Lim,Cheol Seong Jang 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Plant growth under water-deficit conditions adversely affects many key processes. Efforts to understand drought stress-related defense mechanisms have revealed a host of plant genes using molecular approaches in rice. Here, we report the novel finding that OsCTR1 E3 ligase regulates both chloroplast-localized chloroplast protein 12 (OsCP12) and ribosomal protein 1 (OsRP1) in protein levels and subcellular localization. The results of a yeast-two hybrid assay, bimolecular fluorescence complementation assay, ubiquitination assay, subcellular localization, and a protein degradation assay support the hypothesis that OsCTR1 functions in trafficking inhibition and proteolysis of OsCP12 and OsRP1 via the ubiquitin 26S proteasome pathway. Heterogeneous overexpression of OsCTR1 in Arabidopsis showed ABA-hypersensitive phenotype in seed germination, seedling growth, and stomatal closure. The transgenic plants also exhibited improvement of water-deficit tolerance with an accumulation of hydrogen peroxide production. These results demonstrate that the OsCTR1 E3 ligase might positively regulate the cellular functions of OsCP12 and RP1 related to photosynthesis under drought stress conditions in rice.