Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

난소암에서 유래한 피부 전이암 1예

최규철,윤영곤 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.1

One case of metastatic skin tumor from carcinoma of the ovary was reported with literature re-view. A 20-year-old woman had an episode of total hysterectomy and bilateral salpigo-ophorectomy under the diagnoisis of mucinous cystadenocarcinoma about one year ago. About 2 months ago, a nodule developed on the site of operation wound of the abdomen. Thereafter, multiple nodules developed succeedingly on the abdomen, back and scalp. The nodules were considered to be originated from carcinoma of the ovary in the view of histopathological, immunohistochemical and electron-microscopic finding.

政府 單位 實體의 監査에 關한 硏究

崔永坤 啓明大學校 産業經營硏究所 1991 經營經濟 Vol.24 No.1

동양 전통과학의 탐구방법론 연구 : 朱子의 格物致知論을 중심으로

최영제,이우붕,정원우,김용수,문장수,방경곤,김기정 경북대학교사범대학과학교육연구소 2005 科學敎育硏究誌 Vol.29 No.-

분석적 절차의 적용에 관한 연구

최영곤 啓明大學校 産業經營硏究所 2003 經營經濟 Vol.36 No.1

The purpose of this paper is to discuss analytical procedure techniques that involve evaluations of financial statement information by a study of relationships among financial and nonfinancial data. Techniques used in performing analytical procedures include trend analysis, ratio analysis, regression analysis and reasonableness tests. The process of performing analytical procedures consists of four steps: 1. Develop an expectation of an account( or ratio)balance. 2. Determine the amount of difference from the expectation that can be accepted without investigation. 3. Compare the company's account (ratio) balance with the expectation. 4. Investigate and evaluate significant differences from the expectation. Some examples of performing analytical procedures were presented in this study. It is expected that this study provide some guidelines to enhance the availability of analytical procedures for the audit practice.

國際 M&A에 대한 韓國企業의 靜態的 決定要因에 관한 實證的 硏究

崔宗洙,崔永坤 韓國海洋大學校 社會科學大學 1996 韓國海洋大學校 社會科學硏究論叢 Vol.- No.3

은닉부정의 조사방법

최영곤 啓明大學校 産業經營硏究所 2001 經營經濟 Vol.34 No.2

Internal fraud can be broken down into tow categories: employee fraud and fraudulent financial reporting. Fraud can be detected at all three stages: theft act, concealment, conversion. The purpose of this paper is to discuss investigative techniques that focus on concealment efforts. Most frauds are concealed by manipulation source documents, such as purchase invoices, sales invoices, credit memos. Most concealment investigative techniques involve ways to gather these documents. Five investigative techniques are discussed that are used to gather documentary evidence: (1) chance and tips, (2) audits, (3) accessing hard-to-get private documents, (4) document examiners, and (5) searches of public sources.

대구지역 실내 외 공기중 주요 대기오염물질의 농도조사에 관한 연구

최진수,백성옥,김영민,박상곤,정점희,황승만 嶺南大學校 環境問題硏究所 1995 環境硏究 Vol.15 No.1

최근 현대인의 실내거주시간이 일상생활시간의 80% 이상을 차지할 정도로 크게 증가하면서 보건·위생학적 측면에서 실내공기질은 매우 중요한 관심사로 등장하게 되었다. 본 연구는 1994년 8월의 여름철과 1994년 12월 ∼1995년 1월의 겨울철에 대구를 중심으로 가정집, 사무실, 식당과 같은 3가지 유형의 일반주거환경 중 실내·외 공기를 대상으로 실행되었다. 측정항목으로는 RSP, CO, CO₂, NO₂등의 주요 기준성 오염물질과 Bioaerosol을 선정하였다. 본 연구의 결과, 각 성분대상물질의 실내·외 농도는 실내거주환경 및 여름과 겨울, 두 계절에 따라 영향을 받는 것으로 나타났으며 실내·외 농도비교에서 RSP, CO, CO₂의 실내농도는 대부분의 지역에서 실외에 비하여 높은 농도를 나타냈다. 각 실내거주환경에 따른 실내·외의 농도는 가정, 사무실에 비하여 식당에서 상대적으로 높은 농도를 나타냈으며 실내·외 농도비 또한 식당에서 상대적으로 큰 값을 나타냈다. 여름과 겨울의 두 계절에 따른 RSP, CO, CO₂의 실내·외 농도는 여름철에 비하여 겨울철에 더욱 높은 농도를 나타냈으나 Bioaerosol의 실내·외 colony/plate는 여름철에 더욱 높은 수치를 나타냈다. The study was carried out to investigate the concentrations of indoor air pollutants such as respirable suspended particulate (RSP), carbon monoxide (CO), carbon dioxide (CO₂), nitrogen dioxide (NO₂) and bioaerosol at homes, offices and restaurants in Taegu city. Indoor and outdoor concentrations of these pollutants were observed simultaneously from August 1994 for summer to January 1995 for winter. The results were summarized as follows : 1. Both in summer and in winter, the indoor concentrations of RSP, CO, and CO₂; were higher than the outdoor concentrations, the indoor concentrations of major air pollutants (RSP, CO, CO₂, NO₂) in restaurants were higher than those in homes and offices. 2. The indoor and outdoor concentrations of RSP, CO, and CO₂; in winter were significantly higher than those in summer, on the other hand. The CFU/plate of bioaerosol in winter was lower than that in summer. These results suggested that indoor levels of air pollutants were affected by various indoor characteristics such as smoking, cooking, ventilation rate, winter heating systems, and behavioral activity of occupants.

EVA Nanocomposites 제조 및 특성연구

김영용,박일수,정효선,최재곤,김정규,조병욱 조선대학교 생산기술연구소 2003 生産技術硏究 Vol.25 No.1

Ethylene vinyl acetate random copolymer(EVA)/layered-silicate nanocomposites were prepared by melt or solution blending. The TGA thermograms of EVA/ layered-silicate nanocomposites showed an improved thermal stability as the layered-silicate contents increased. The decomposition temperature of nanocomposite was increased around 10℃ compared with pure EVA at 50% weight loss under N_2 atmosphere And in an air atmosphere, temperature difference in 50% wt loss between the nanocomposlte and the virgin EVA was around 100℃ The moduli in their thermomechanical module of nanocomposite are higher than pure EVA. Interlayered spacings of nanosilicates were slightly increased when prepared by melt blending. On the other hand, they were remarkably increased when prepared by solution blending. It may be explained that EVA moiety of the copolymer showed partial adsorption behavior on the surface layer of the silicate. It also revealed that the adsorption is more effective in the solution blending than in the melt blending.

인터넷 비즈니스 보안의 과제와 대책 : Related issues and recommendations

도상호,최영곤 啓明大學校 産業經營硏究所 1999 經營經濟 Vol.32 No.2

SUMMARYInternet allows access to anyone and uses of the Internet for business purpose(e-business) are growing rapidly. If the world were a utopia with no criminals, the Internet would be an ideal workplace for business. As it is, however, the very features that made the network powerful also created security risks.This paper identifies Internet Business-related security risk factors and typical hacking techniques such as packet sniffing and IP spoofing. Internet Business-related security risks are classified into systems security factors and network security factors. It also discusses investigative and preventive measures to deter them. These measures include fire wall, development of strategies for security, security budget, hiring security consultants and on-going training for personnels. Security is without question the weakest link in the Internet and will be the key for success in e-business in the new millenium.