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Polymerase chain reaction으로 설사 영유아 대변 검체에서 adenovirus type 40, 41의 분리 동정
박혜경,서주영,정영해,김경희 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-
Adenovirus is the second leading cause of acute gastroenteritis. As the children with diarrhea by acute adenoviral gastroentertis usually require hospitalization, rapid diagnosis is necessary for proper management. Adenovirus infection can be diagnosed conventionally by the direct electron microscopic observation of the virus particle, isolation of the virus by cell culture, or enzyme immuno assay. Recently, polymerase chain reaction is applied for the detection of adenovirus. In this study, we investigated stools collected from 100 children hospitalized for diarrhea in Ewha womans University Hospital from the September of 1995 to the July of 1996 for adenovirus by polymerase chain reaction. Two oligonucleotide primers, desiginated as hexAA1885 and hexAA1913, were used for the detection of enteric adenovirus type 40 an 41 in PCR. After amplification pf 35 cycles, 300 bp fragments were detected on 1.8% Sea Kem GT agar gel in 14 samples. The stool samples were further analyzed by electron microscopy and immunoblotting. In electron microscopy, icosahedral particles of about 75nm were observed in a^7 of the 14 samples. The 14 samples were collected from children whose mean age was 1.4 years and the sex ratio was 10/4(M/F). The seasonal variation showed acute gastroenteritis by adenovirus infection was frequent in March to May and in September to October in Korea.
유치 수복물에 따른 주변 법랑질의 탈회 저항성 : QLF를 이용한 연구
권해숙,현홍근,김영재,김정욱,장기택,김종철,한세현,이상훈 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.1
본 연구에서는 유치 수복물의 불소 방출 여부에 따른 주변 법랑질의 탈회 저항성과 재광화 효과를 살펴보고자 하였다. 건전 유전치 48개를 16개씩 임의로 세 군으로 나누어, FiltekTM Z250(1군), F2000(2군), KetacTM N100(3군)을 사용하여 제조사의 지시에 따라 충전하였다. 3일 동안 인공 우식 병소를 유발한 후, 14일 동안 인공 타액에 담가 재광화를 유발하였다. Quantitative light-induced fluorescence(QLF)를 이용하여 실험 단계와 시간에 따른 무기질 소실량(ΔQ)의 변화를 관찰한 결과는 다음과 같다. 1. 탈회가 일어난 인공 우식 병소의 ΔQ 값은 3군, 2군, 1군 순으로 작게 나타났고, 이 중 3군은 1군에 비해 유의한 탈회 저항성을 보였다. 2. 1군, 2군, 3군 모두 재광화 1일 후부터 초기 인공 우식 병소의 ΔQ 값과 비교 시 유의한 차를 보였으며, 관찰 기간 동안 지속적인 증가 양상을 나타내었다. 3. 재광화 속도를 의미하는 Δ(ΔQ)/일 값은 각 군에서 처음 1일 동안 유의성 있게 크게 나타났으며, 그 이후 급속히 감소하였다. 4. 수복 재료에 따른 재광화 정도는 각 군 간에 유의한 차이가 없었다. The objective of this in vitro study was to detect and monitor demineralization and remineralization of primary teeth according to restorative materials using quantitative light-induced fluorescence (QLF). A single bur hole was drilled on the each sound forty eight primary anterior teeth, and the specimens were divided into three groups. The cavity was restored with FiltekTM Z250(Group 1), F2000(Group 2), KetacTM N100(Group 3) following the manufacturer's instructions. The teeth were subjected to the demineralizing buffer for 3 days, and then subjected to a remineralizing buffer for 14 days. The change of mineral loss(ΔQ) according to the stages was evaluated by QLF and the following results were obtained: 1. When demineralization was done, ΔQ was increased as follows. : Group 1 (-110.79 ± 27.77) < Group 2 (-104.84 ± 28.95) < Group 3 (-90.16 ± 21.87) : Resistance to demineralization was statistically significant in Group 3. 2. There was a statistically significant increase in ΔQ of all groups since 1st day of remineralization 3. The rate of remineralization, Δ(ΔQ)/day, showed significant high value in each group on the 1st day then decreased rapidly. 4. There was no statistically significant difference in the degree of remineralization among restorative materials.
권해숙,현홍근,김영재,김정욱,장기택,김종철,한세현,이상훈 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.4
Tooth transposition is an anomaly of eruption characterized by the interchanged positions of two adjacent teeth, which is divided into complete and incomplete transposition. There are three common approaches for treating transposition: aligning the involved teeth in their transposed positions, moving them to their correct anatomic position in the arch and extracting one of the transposed teeth. Considerations in treatment plans are esthetic, function, risk of jeopardizing the roots and damaging the supporting structures, position of the root apex, developmental stages of teeth and expected compliance. The presented case reports described one maxillary canine-the first premolar transposition and two mandibular lateral incisor-canine transpositions. The former transposed teeth were arranged in their transposed position, and the latter transposed teeth were rearranged into their normal position. 치아전위란 인접한 두 치아의 위치가 서로 바뀐 치아 위치 이상을 말하며, 치관과 치근의 위치에 따라 완전전위와 불완전전위로 나눌 수 있다. 하악보다는 상악에 편측성으로 호발하며, 특히 상악 견치에서 자주 발생한다. 전위는 발견 시기와 전위된 양상에 따라 치료방법이 달라질 수 있다. 본 증례는 상악 견치와 제1소구치 간의 전위가 일어난 10세 7개월의 환아와 하악 측절치와 견치 간의 전위가 일어난 8세 11개월 환아와 8세 9개월 환아의 교정치료에 대한 것으로 양호한 치료 결과를 얻었고, 증례 별로 치료방법의 차이점에 관한 고찰을 하였기에 이를 보고하는 바이다.
Aggregation of amyloid precursor proteins by aluminum in vitro
Chong, Young-Hae,Suh, Yoo-Hun 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-
The effect of various metal ions on aggregation of human recombinant amyloid precursor protein(APP)in vitro was investigated based on characterizations of altered migration on SDS-PAGE or immunoblots. Most biological metal ions tested had no significant effect on aggregation of APP. In contrast, AICI_3 in particular promoted aggregation of APP or APP-CT105 in a dose dependent manner. This effect of AICI_3 on APP mobility shift was prevented or reversed by the metal chelator, EDTA. Amorphous aggregates were observed in AICI_3 treated APP when examined by EM. These results suggest that aluminum may play a role in the pathogenesis of AD directly promoting aggregation of APP.
Chong, Young Hae,Suh, Yoo-Hun 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-
Recent studies implicate that excessive amyloidogenic pathway of amyloid precursor protein(APP)processing may be the final commom pathway involved in the pathogensis of AD. In attepmts to identify the proteases or factors leading to excessive amyloid deposition, we evaluated the potential role of acethylcholinesterase(AChE)and its associated protease for amyloidogenic processing of APP in vitro. Prolonged incubation of a recombinant APP770 with AChE produced several amyloidogenic fragments accumulating a relatively stable a 18 kDa Aβ(amyloidβ-protein)bearing carboxy terminal peptide, which was further degraded by an increased concentration of AChE. Protease inhibitory profiles confirmed the trypsin-like serine protease activity present in AChE preparation. This observed APP processing was significantly enhanced by Ca^2+, Mg^2+, or Mn^2+ at 1 mM concentration and modulated in concentration dependent manners by metal ions such as Ca^2+, Zn^2+, Fe^2+/Fe^3+, Al^3+, or a tacrine, a centrally active cholinesterase inhibitor. Our data imply that AChE and its associated protease may be involved in the generation a 18 kDa amyloidogenic peptide under certain physiological condition in vivo and that the gradual changes in their proteolytic activities or locations and the locally disturbed metal homeostasis could be factors associated with abnormal accumulation of APP, evantually leading to amyloid deposition in AD brain. In addition, zinc or tacrine treatment of AD patients with high dosage or in the long term may have effects on the process of amyloidogensis.