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Naohiro Nishikawa,Katsuhiko Omoe,Kenji Murakami,Yusuke Sato,Takekazu Sawa,Yoshihiro Hagihara,Nobuhito Yoshihara,Hiroaki Okawai,Toshirou Iyama,Masahiro Mizuno,Shinya Tsukamoto 한국정밀공학회 2013 International Journal of Precision Engineering and Vol. No.
It is great concern that is environmental load in manufacturing. In machining site, metal working fluid (coolant) such as cutting oil,grinding fluid is used. It contains several chemicals that are oil, surface active agent, extreme pressure additive, antirust agent,antifoaming agent, preservative, biocide etc.. It is thought that it is not good for environment and human body. Machining fluid mist and splash contained several chemicals that are cause of danger for worker’s health while machining. Furthermore, time elapses, fluid is rotten by bacteria. Bad smell and degradation of machining performance occurs. Therefore, after using machining fluid, waste fluid is disposed. Incineration or coagulative precipitation and landfill etc. are necessary. It arise great disposal cost and environmental load as discharging of huge amount green house gas (CO2 etc.). Then, machining fluid decreasing or non-using is demanded in industry. So, in this investigation, new environmental machining method: the electric rust preventive machining method system that uses only water as machining fluid has been developed. In this paper, water purification recycle system in water machining system development is mentioned. Therefore, complete removal of bacteria (Enterobacter aerogenes) without using chemicals such as biocide is examined for corruption, malodor prevention and safe machining water.
Motokawa, Shogo,Narasaki, Yukie,Song, Jun-Young,Yokoyama, Yoshihiro,Hirose, Euichi,Murakami, Shoko,Jung, Sung-Ju,Oh, Myung-Joo,Nakayama, Kei,Kitamura, Shin-Ichi Elsevier 2018 Parasitology international Vol.67 No.2
<P><B>Abstract</B></P> <P>The ciliate <I>Miamiensis avidus</I> causes scuticociliatosis in Japanese flounder <I>Paralichthys olivaceus</I>. We previously reported three serotypes of this ciliate distinguishable by serotype-specific antigenic polypeptides (serotype I, 30kDa; serotype II, 38kDa; serotype III, 34kDa). In this study, we determined the localization site of the serotype-specific polypeptides in the ciliate and determined the genes encoding the polypeptides, using the isolates IyoI (serotype I), Nakajima (serotype II), and Mie0301 (serotype III). SDS-PAGE and immunoblot analysis of cilia, membrane proteins, and cytoskeletal elements of the ciliates revealed that the polypeptides were abundant in the former two. Scanning electron microscopy of ciliates immobilized by homologous antiserum showed morphological changes in the cilia. These evidences suggested that the polypeptides were ciliary membrane immobilization antigens. The ciliary genes identified showed low identity scores—<51.5% between serotypes. To differentiate the serotypes, we designed serotype-specific PCR primer sets based on the DNA sequences. The PCR-based serotyping results were completely consistent with conventional serotyping methods (immobilization assay and immunoblot analysis). Twenty of 21 isolates were classified as either serotype I or II, and one isolate was undistinguishable. The combination of species-specific PCR previously reported and three serotype-specific PCR could be useful for identifying, serotyping, and surveillance for occurrences of new serotypes of <I>M. avidus</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Three serotypes of <I>M. avidus</I> were reported in Japan and Korea. </LI> <LI> Serotype-specific polypeptides were identified as ciliary membrane proteins. </LI> <LI> The nucleotide sequences of ORFs were determined. </LI> <LI> Serotype-specific PCR revealed that the pandemic serotypes were serotype I and II. </LI> </UL> </P>
Mina Jung,Sunyeo Ha,Seungjun Oh,구지은,Keisuke Kobayashi,Tae-In Jeon,Yoshihiro Murakami,Jiho Chang,Takafumi Yao 한국물리학회 2009 Current Applied Physics Vol.9 No.2
ZnO:In nanocrystals (NCs) with various In contents were synthesized by using a mixed source vapor transportation method. The morphological variation and electronic structure of ZnO:In NCs were studied by bulk sensitive hard X-ray photoemission spectroscopy (HX-PES). ZnO:In NCs are classified into ZnOlike hexagonal and In2O3-like cubic groups, based on the crystal structure, and we have estimated the In content for the phase transformation to be as high as 35 atomic% (atm.%). A filled electronic state in the conduction band was observed from the ZnO-like group, which is regarded as a metallic state, due to an increase of extrinsic carrier density. ZnO:In nanocrystals (NCs) with various In contents were synthesized by using a mixed source vapor transportation method. The morphological variation and electronic structure of ZnO:In NCs were studied by bulk sensitive hard X-ray photoemission spectroscopy (HX-PES). ZnO:In NCs are classified into ZnOlike hexagonal and In2O3-like cubic groups, based on the crystal structure, and we have estimated the In content for the phase transformation to be as high as 35 atomic% (atm.%). A filled electronic state in the conduction band was observed from the ZnO-like group, which is regarded as a metallic state, due to an increase of extrinsic carrier density.
Toyota Kazuhiro,Mori Masayuki,Hirahara Satoshi,Yoshioka Shoko,Kubota Haruna,Yano Raita,Kobayashi Hironori,Hashimoto Yasushi,Sakashita Yoshihiro,Yokoyama Yujiro,Murakami Yoshiaki,Miyamoto Katsunari 대한위암학회 2022 Journal of gastric cancer Vol.22 No.1
Purpose: Nutritional problems after gastrectomy affect continuation of postoperative chemotherapy. There have been no studies limited to total gastrectomy, which is particularly prone to nutritional problems. In this study, we aimed to investigate the factors that predict the continuation of postoperative chemotherapy. Materials and Methods: We included 101 patients who underwent curative total gastrectomy and postoperative chemotherapy at Hiroshima Memorial Hospital. The effects of 37 factors, including perioperative inflammatory, nutritional, and tumor status, on the persistence of postoperative chemotherapy were analyzed. Results: In univariate analysis of preoperative factors, age, carbohydrate antigen 19-9, platelet-to-neutrophil ratio, Onodera's prognostic nutritional index (PNI), controlling nutritional status score, and nutritional risk screening (NRS-2002) score were significantly associated with the duration of postoperative chemotherapy. In multivariate analysis of preoperative factors, age (≥74 years) was an independent factor for a shorter duration of postoperative chemotherapy (hazard ratio [HR], 5.24; 95% confidence interval [CI], 2.19–12.96; P<0.01). In univariate analysis of factors before postoperative chemotherapy, intraoperative blood loss, perioperative weight loss rate, postoperative performance status, PNI, albumin-to-bilirubin index, and NRS-2002 score were significantly associated with the duration of postoperative chemotherapy. In multivariate analysis of factors before postoperative therapy, age (≥74 years) (HR, 5.75; 95% CI, 1.90–19.49; P<0.01) and PNI (<39) (HR, 3.29; 95% CI, 1.26–8.56; P=0.02) were independent factors for a shorter duration of postoperative chemotherapy. Conclusions: Age and PNI are useful predictors of postoperative chemotherapy intolerance after total gastrectomy and may determine the treatment strategy and timing of chemotherapy initiation.