Brief Introduction on Standardization for Water Sector in China

Liu Yongfeng 한국수자원학회 2005 한국수자원학회 학술대회 Vol.2005 No.1

Molecular Cloning, Expression and Characterization of Bovine UQCC and Its Association with Body Measurement Traits

Liu, Yongfeng,Zan, Linsen,Zhao, Shuanping,Huang, Honggang,Li, Yong,Tang, Zhonglin,Yang, Shulin,Li, Kui Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.5

Ubiquinol-cytochrome c reductase complex chaperone (UQCC) involved in the development and maintenance of bone and cartilage is an important candidate gene for body measurement traits selection through marker-assisted selection (MAS). The expression of UQCC is upregulated in many human and animal models of height as well as other stature indexes. We have cloned the cDNA sequence coding UQCC gene in bovine. Genomic structural analysis indicated that bovine UQCC shares a high similarity with human UQCC. Furthermore, Real-Time PCR analysis showed that the expression of bovine UQCC is remarkably different in diverse tissues, including high level expression in the spleen, heart and windpipe, and relatively low expression in other tissues. We also analyzed allele frequencies in different cattle breeds and an association study on the selected SNPs. SNP DraI A2691T in intron 1 and SNP Bsh1236I A3150G in intron 8 are significantly associated with Body Length (BL), Rump Length (RL), Chest Depth (CD) and Pin Bone Width (PBW). For the A2691T SNP marker, there are significant effects on the RL (p = 0.0001), CD (p = 0.0059) and PBW (p < 0.0001) in 679 individuals; with A3150G SNP marker, there are significant effects on the BL (p = 0.0047) and CD (p = 0.0454). Regarding association analysis of combination of the two SNPs, there are significant effects on the BL (p = 0.0215), CD (p = 0.0282) and PBW (p = 0.0329) in the total population. The results suggest that the UQCC gene is a candidate gene of body measurement traits in bovine reproduction and breeding, and provide data for establishing an animal model using cattle to study big animal body type.

Enterprise Computer Network Reliability Analysis

Yongfeng Cui,Wei Liu,Ya Li 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.1

This paper considers the multi-protocol label switching enterprise computer network routing optimization problem, in the network reliability constraints, by selecting the optimal work and alternate paths, and carries on the bandwidth allocation; network total construction cost is minimal. Due to the total construction cost and network reliability is related to choice of the length of the path, the optimal route network design problem can be decoupled into two sub-problems: cost and reliability related issues, among them, the cost of related sub problem study, Sohn et al. For reliability related problems, reduce the search space understanding, this chapter will work best and standby path search space from a hyper plane reduce to the hyper plane line; Then, reliability related algorithm is proposed to solve the problem. Because of the cost and reliability related sub-problem solutions are obtained based on the same search criterion, so the original problem of optimal work and backup path of solution set is by comparing the two sub problems solution to work out. Finally, related algorithm provides a numerical example to verify the reliability and the validity of the global network routing design method.

Cheese Manufacturing and Bioactive Substance Separation: Separation and Preliminary Purification of cAMP from Whey

Yongfeng Liu,Xiaowei Zhao,Manshun Liu,Jing Zhao 한국축산식품학회 2018 한국축산식품학회지 Vol.38 No.1

Cheese consumption has been gradually increased in China. However, both the manufacturing process of cheese and the utilization of its main by-product were not well developed. Based on the sensory evaluation, Box-Behnken Design (BBD) was performed in the present study to optimize the cheese processing, which was proved more suitable for Chinese. The optimal parameters were: rennet 0.052 g/L, start culture 0.025 g/L and CaCl2 0.1 g/L. The composition analysis of fresh bovine milk and whey showed that whey contained most of the soluble nutrients of milk, which indicated that whey was a potential resource of cyclic adenosine-3’, 5’-monophosphate (cAMP). Thus, the cAMP was isolated from whey, the results of high-performance liquid chromatography (HPLC) analysis showed that the macroporous adsorption resins (MAR) D290 could increase the concentration of cAMP from 0.058 μmol/mL to 0.095 μmol/mL. We firstly purified the cAMP from the whey, which could become a new source of cAMP.

Molecular Cloning, Expression and Characterization of Bovine UQCC and Its Association with Body Measurement Traits

Yongfeng Liu,Linsen Zan,Shuanping Zhao,Honggang Huang,Yong Li,Zhonglin Tang,Shulin Yang,Kui Li 한국분자세포생물학회 2010 Molecules and cells Vol.30 No.5

Ubiquinol-cytochrome c reductase complex chaperone (UQCC) involved in the development and maintenance of bone and cartilage is an important candidate gene for body measurement traits selection through marker-assisted selection (MAS). The expression of UQCC is upregulated in many human and animal models of height as well as other stature indexes. We have cloned the cDNA sequence coding UQCC gene in bovine. Genomic structural analysis indicated that bovine UQCC shares a high similarity with human UQCC. Furthermore, Real-Time PCR analysis showed that the expression of bovine UQCC is remarkably different in diverse tissues, including high level expression in the spleen, heart and windpipe, and relatively low expression in other tissues. We also analyzed allele frequencies in different cattle breeds and an association study on the selected SNPs. SNP DraI A2691T in intron 1 and SNP Bsh1236I A3150G in intron 8 are significantly associated with Body Length (BL), Rump Length (RL), Chest Depth (CD) and Pin Bone Width (PBW). For the A2691T SNP marker, there are significant effects on the RL (p = 0.0001), CD (p = 0.0059) and PBW (p < 0.0001) in 679 individuals; with A3150G SNP marker, there are significant effects on the BL (p = 0.0047) and CD (p = 0.0454). Regarding association analysis of combination of the two SNPs, there are significant effects on the BL (p = 0.0215), CD (p = 0.0282) and PBW (p = 0.0329) in the total population. The results suggest that the UQCC gene is a candidate gene of body measurement traits in bovine reproduction and breeding, and provide data for establishing an animal model using cattle to study big animal body type.

Application of Improved BP Neural Network with Correlation Rules in Network Intrusion Detection

Yongfeng Cui,Xiangqian Li Ma,Zhijie Liu 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.4

To detect various network attacks in real time, this paper developed a network intrusion detection system based on artificial neural network. This paper first introduced the recent development of neural network, BP algorithm and structure of a simple perceptron. Then, this paper developed an improved BP neural network algorithm to detect anomaly network traffic with adjusted correlation rules. Finally, the network intrusion system in this paper was tested in a real network situation; the improved BP algorithm neural network with adjusted correlation rules shows a reduction in total error and increment in alarm rate compared to the traditional basic BP algorithm model.

Stimuli-Responsive Polymers: Fundamental Considerations and Applications

Yongfeng Gao,Menglian Wei,Xue Li,Wenwen Xu,Andrews Ahiabu,Juliana Perdiz,Zining Liu,Michael J. Serpe 한국고분자학회 2017 Macromolecular Research Vol.25 No.6

Stimuli-responsive polymers are capable of changing their chemical and/ or physical properties in response to environmental stimuli. This unique feature has allowed stimuli-responsive polymers to be used in a variety of applications. In this review, we present a basic introduction to the theories that have been developed to describe polymer chains, brushes, and networks. We then detail numerous examples of how stimuli-responsive polymers can be used for sensing and biosensing, drug delivery, and as artificial muscles. While we focus the review on these particular areas, there are numerous other demonstrations of the applications of these fascinating materials, and we are certain that many applications have yet to be discovered.

STATUS QUO AND PROBLEM ON LEACHATE MANAGEMENT OF MSW LANDFILL IN CHINA

( Nie Yongfeng ),( Liu Jianguo ) 한국폐기물자원순환학회 2002 APLAS Vol.2002 No.1

중국의 도시고형폐기물매립지의 침출수 관리 현황 및 문제점

( Nie Yongfeng ),( Liu Jianguo ) 한국폐기물자원순환학회 2002 APLAS Vol.2002 No.2

경제발달, 도시화와 생활수준 향상에 따라 중국의 일반폐기물이 계속적으로 증가하고 있다. 일반 폐기물은 1980년에 3,132만톤에서 1996년 10,825만톤으로 증가하였고, 2000년이 되어서는 12~14 억톤으로 연평균 8.1%의 증가율을 보였다. 일반폐기물의 낮은 재활용율과 발열량으로 인해서, 매립이 중국의 상황에 있어 가장 적절한 처리기술이다. 최근 10년간, 많은 일반폐기물 매립지가 건설되었다. 이로서 일반폐기물의 불법투기와 단순매립은 점차적으로 사라지고 있다. 매립지 설계, 건설, 관리는 위생매립지 수준을 만족시키기 위해서 끊임 없이 개선되었다. 침출수 발생에 의한 환경오염이 매립지에 있어서 주요한 환경문제로, 침출수 발생 제어가 매립지의 설계, 건설, 운영에 있어서 우선적으로 고려되어야 한다. 일반폐기물매립지 침출수는 많은 물질을 함유한 일종의 고농도의 폐수이다. 침출수 발생과 그 성분의 강한 변동은 매립지 침출수 처리를 여전 히 풀지 못한 숙제로 남게 하였다. 심지어 침줄수가 생물학적 호기-혐기 기술에 의해서 처리된다고 하여도 방류기준을 충족시키지 못한다. 수질에 관심을 가지는 한, 경제적이고 실행 가능한 기술인 침 출수 재순환도 작은 양의 침출수가 발생하는 매립지에 있어서만 효과적이다. 다량의 침출수 발생은 중국에서 매립지 설계, 건설, 운영에 있어서 고려되어야만 하는 중요한 문 제이다. 침출수 처리시설의 건설, 운영에 필요한 재정의 부족으로 많은 매립지는 적절한 침출수 처리 시스템을 갖추지 못하고 있다. 게다가 고농도의 침출수는 처리하기 어렵기 때문에, 처리시설을 거친 침출수 역시 국가 혹은 지역의 기준을 거의 충족시키지 못한다. 효과적인 배수시스템을 갖추지 못한 몇몇 매립지는, 단지 작은 양의 침출수만을 수집한다. 따라서 많은 양의 침출수가 토양에 침투된다. 기술적, 경제적, 환경적인 면을 고려한다면, 침출수 제어는 오염원 감량 측면에서 이루어져야 한다. 이들을 만족시키기 위한 경제적이고 효과적인 기술들, 예로 침출수 재순환에 대해서 연구하였다.

Experimental Study on the Longitudinal Mechanical Behavior of Shield Tunnel in Soft-Hard Uneven Strata and the Reinforcement Effect of Longitudinal Channel Steel

Tingjin Liu,Shuyi Zhang,Yongfeng Tang,Zhenwei Ye 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.1

This paper investigates the longitudinal behavior and failure pattern of shield tunnel, as well as the reinforcement mechanism of longitudinal channel steel, using reduced-scale tunnel models assembled with 24 lining rings. The longitudinal behavior of the shield tunnel and the reinforcement effect are discussed in terms of vertical deformation, joint deformation, and concrete cracking. The results indicate that the shield tunnel without reinforcement longitudinallydeforms in a “bending and dislocation” mode, with the failure pattern dominated by bending deformation. In contrast, the failure pattern of the shield tunnel reinforced by channel steels is characterized by brittle shearing dislocation fracture of the circumferential joint. The load at which damage occurs in the reinforced tunnel model is 1.74 times and 2.04 times that of the non-reinforced staggered and straight jointed tunnels. The reinforcement reduces the horizontal convergence discrepancy of lining rings above the soft foundation spring and the adjacent lining rings, which improves the overall integrity of the tunnel structure.