The Evolution of Living Donor Liver Transplantation for Alcoholic Liver Cirrhosis in a High Volume Center: The Eastern Perspective

( Jeffrey Samuel Co ),( Chih-chi ),( Wang ),( Chih-che Lin ),( Chee-chien Yong ),( Chao Long Chen ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Alcoholic liver disease (ALD) has been a growing indication for living donor liver transplantation (LDLT) in Asia but certain medical, ethical and psycho-social issues question its appropriateness. Reports on long-term outcomes of LDLT for ALD are also scarce and so our aim was to report our center’s experience for the past 15 years and how LDLT has evolved to be the treatment of choice for end-stage liver disease secondary to ALD in a high volume center. Methods: A total of 1,384 consecutive LDLT was performed from January 2003 to August 2016 at Kaohsiung Chang Gung Memorial Hospital, and 87 patients had a pre-operative diagnosis of alcoholic liver disease (ALD) with or without hepatocellular carcinoma (HCC). This group was systematically matched with non-ALD (NALD) patients in a ratio of 1:2 using equiprobability method. Overall patient survival was compared using Kaplan-Meier analysis, and incidences of post-transplant De novo malignancy and alcohol relapse were described. Results: Patient demographics were comparable, as well as preoperative and intra-operative data. Of the 87 patients in the ALD group, 26 (30%) had concomitant HCC. Median follow- up for this study was 50 months. Overall patient survival at 1, 3 and 5 years for ALD were 98%, 97% and 92% respectively, while the NALD group had similar survival rates (P =0.282). The rate for De novo malignancy was 6% while that for recidivism was 7% despite only 76% of the patients meeting the 6 months abstinence rule. Conclusions: Results from our center show that LDLT for ALD has comparable short and longterm outcomes when compared to NALD, and the close relationship between donor and recipient seems to positively affect alcohol relapse rate and patient compliance to medication.

신공법에 의해 제조한 흑삼의 아토피 억제효과

김의검,이지현,신귀남,조수현,김룡국,이지숙,박용진,윤치영,송규용 충남대학교 약학대학 의약품개발연구소 2008 藥學論文集 Vol.23 No.-

The purpose of this study was to investigate the effects of crude saponin fraction of black ginseng(SFBG) on atopic dermatitis in vitro. The house dust mite(Dermatophagoides pteronissinus, DP) plays a key role in the pathogenesis of allergic diseases, including atopic dermatitis and asthma. Monocyte chemo-attractant protein 1(MCP-1/CCL2)/interleukin-6(IL-6)/IL-8(CXCL8) plays a pivotal role in mediating the infiltration of various cells into the skin of atopic dermatitis. We found that SFBG potently suppressed the elevated production of MCP-1, IL-6 and IL-8 induced by D. pteronissinus extract(DpE) treatment in the human monocytic cell line, THP-1 cells. Also, SFBG potently suppressed the elevated production of IL-6 and IL-8 induced by DpE treatment in the human eosinophilic cell line, EoL-1 cells. These results suggest that SFBG have an anti-atopic property, and that in future further development of main effective constituents in SFBG can provide a novel therapeutic agent for atopic dermatitis.

<i>Caulobacter daechungensis</i> sp. nov., a stalked bacterium isolated from a eutrophic reservoir

Jin, Long,Lee, Hyung-Gwan,Kim, Hee-Sik,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.7

<P>A Gram-stain-negative, aerobic, non-spore-forming, curved, rod-shaped bacterium, H-E3-2<SUP>T</SUP>, was isolated from a water sample taken from Daechung Reservoir, Republic of Korea, during the late-blooming period of cyanobacteria. Strain H-E3-2<SUP>T</SUP> was motile with a single polar flagellum or non-motile (stalked cell). Comparative 16S rRNA gene sequence studies showed the isolate had a clear affiliation with the class <I>Alphaproteobacteria</I> and was most closely related to <I>Caulobacter fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>Caulobacter mirabilis</I> LMG 24261<SUP>T</SUP>, showing 97.6 and 97.3 % 16S rRNA gene sequence similarity, respectively, and 95.3–96.3 % similarity to all other species of the genus <I>Caulobacter</I>. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (C<SUB>18 : 1</SUB>ω6<I>c</I> and/or C<SUB>18 : 1</SUB>ω7<I>c</I>) and C<SUB>16 : 0</SUB>. The G+C content of the genomic DNA of strain H-E3-2<SUP>T</SUP> was 64.7 mol%. DNA–DNA hybridization values of strain H-E3-2<SUP>T</SUP> with <I>C. fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>C. mirabilis</I> LMG 24261<SUP>T</SUP> were 21.2 and 19.7 %, respectively. Thus, based on the results of polyphasic analysis, it is proposed that strain H-E3-2<SUP>T</SUP> represents a novel species of the genus <I>Caulobacter</I>, for which the name <I>Caulobacter daechungensis</I> sp. nov. is proposed. The type strain is H-E3-2<SUP>T</SUP> ( = KCTC 32211<SUP>T</SUP> = JCM 18689<SUP>T</SUP>).</P>

<i>Aquihabitans daechungensis</i> gen. nov., sp. nov., an actinobacterium isolated from reservoir water

Jin, Long,Huy, Hangsak,Kim, Kwang Kyu,Lee, Hyung-Gwan,Kim, Hee-Sik,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.8

<P>A novel Gram-reaction-positive bacterium, strain CH22-21<SUP>T</SUP>, was isolated from a water sample taken from Daechung Reservoir, Republic of Korea, during the late-blooming period of cyanobacteria. Cells of strain CH22-21<SUP>T</SUP> were non-motile, ciliated short rods that formed creamy-white colonies on half-strength modified R2A agar. Chemotaxonomic results showed menaquinone MK-9(H<SUB>6</SUB>) as the predominant respiratory menaquinone, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside as major polar lipids, 16 : 1ω5<I>c</I>, 16 : 0, 17 : 1ω8<I>c</I> and 18 : 1ω9<I>c</I> as major fatty acids, and a DNA G+C content of 71.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain formed a separate lineage within the order <I>Acidimicrobiales</I>, showing similarity values of <92.3 % with its closest phylogenetic neighbours. The combined genotypic and phenotypic data showed that strain CH22-21<SUP>T</SUP> could be distinguished from all genera within the order <I>Acidimicrobiales</I> and represented a novel species of a new genus in the family <I>Iamiaceae</I>, for which the name <I>Aquihabitans dachungensis</I> gen. nov., sp. nov. is proposed. The type strain of <I>Aquihabitans dachungensis</I> is CH22-21<SUP>T</SUP> ( = KCTC 19849<SUP>T</SUP> = JCM 17787<SUP>T</SUP>).</P>

<i>Flaviflexus salsibiostraticola</i> sp. nov., an actinobacterium isolated from a biofilm reactor

Jin, Long,Ko, So-Ra,Lee, Hyung-Gwan,Kim, Byung-Hyuk,Kim, Hee-Sik,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.9

<P>A Gram-stain-positive, aerobic, non-motile, non-spore-forming, cocci-shaped actinobacterium, designated strain EBR4-1-2<SUP>T</SUP>, was isolated from a biofilm reactor in Korea. Comparative 16S rRNA gene sequence studies showed the isolate was clearly affiliated with the class <I>Actinobacteria</I>, and was related most closely to <I>Flaviflexus huanghaiensis</I> H5<SUP>T</SUP>, showing 98.9 % similarity. Cells of strain EBR4-1-2<SUP>T</SUP> formed yellow colonies on R2A agar, contained MK-9(H<SUB>4</SUB>) as the predominant menaquinone, and included C<SUB>18 : 1</SUB>ω9<I>c</I>, C<SUB>16 : 0</SUB>, C<SUB>16 : 1</SUB>ω9<I>c</I> and C<SUB>14 : 0</SUB> as the major fatty acids. The cell-wall peptidoglycan type was A5α (<SMALL>l</SMALL>-Lys–<SMALL>l</SMALL>-Ala–<SMALL>l</SMALL>-Lys–<SMALL>d</SMALL>-Glu). The G+C content of the genomic DNA of strain EBR4-1-2<SUP>T</SUP> was 65.6 mol%. Thus, the combined genotypic and phenotypic data supported the conclusion that strain EBR4-1-2<SUP>T</SUP> represents a novel species of the genus <I>Flaviflexus</I>, for which the name <I>Flaviflexus</I> <I>salsibiostraticola</I> sp. nov. is proposed. The type strain is EBR4-1-2<SUP>T</SUP> ( = KCTC 33148<SUP>T</SUP> = JCM 19016<SUP>T</SUP>).</P>

<i>Hymenobacter ruber</i> sp. nov., isolated from grass soil

Jin, Long,Lee, Hyung-Gwan,Kim, Song-Gun,Lee, Keun Chul,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.3

<P>A taxonomic study using a polyphasic approach was performed on a Gram-stain-negative, aerobic, non-motile, non-spore-forming, rod-shaped bacterium, designated strain PB156<SUP>T</SUP>, isolated from grass soil. Comparative 16S rRNA gene sequence studies showed that the isolate was clearly affiliated with the phylum <I>Bacteroidetes</I>, and most closely related to <I>Hymenobacter soli</I> PB17<SUP>T</SUP>, <I>Hymenobacter antarcticus</I> VUG-A42aa<SUP>T</SUP> and <I>Hymenobacter glaciei</I> VUG-A130<SUP>T</SUP>, showing 96.4, 96.2 and 95.9 % 16S rRNA gene sequence similarity, respectively, while all other species of the genus shared only 89.3–95.2 % similarity. The main polyamine present was <I>sym</I>-homospermidine. The predominant menaquinone was MK-7. The major fatty acids were C<SUB>15 : 0</SUB> iso, summed feature 3 (C<SUB>16 : 1</SUB>ω6<I>c</I> and/or C<SUB>16 : 1</SUB>ω7<I>c</I>/<I>t</I>), C<SUB>16 : 1</SUB>ω5<I>c</I> and C<SUB>15 : 0</SUB> anteiso. The G+C content of the genomic DNA of strain PB156<SUP>T</SUP> was 61.7 mol%. The combined genotypic and phenotypic data supported the conclusion that strain PB156<SUP>T</SUP> represents a novel species of the genus <I>Hymenobacter</I>, for which the name <I>Hymenobacter ruber</I> sp. nov. is proposed. The type strain is PB156<SUP>T</SUP> ( = KCTC 32477<SUP>T</SUP> = JCM 19433<SUP>T</SUP>).</P>

Ferruginibacter profundus sp. nov., a novel member of the family Chitinophagaceae, isolated from freshwater sediment of a reservoir

Jin, Long,Lee, Hyung-Gwan,La, Hyun-Joon,Ko, So-Ra,Ahn, Chi-Yong,Oh, Hee-Mock Springer-Verlag 2014 Antonie van Leeuwenhoek Vol.106 No.2

<i>Caulobacter</i> <i>profunda</i> sp. nov., isolated from deep freshwater sediment

Jin, Long,La, Hyun-Joon,Lee, Hyung-Gwan,Lee, Jay Jung,Lee, Sanghyup,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.3

<P>The Gram-stain-negative, aerobic, non-spore-forming, motile, with a single polar flagellum, or non-motile (stalked) and rod-shaped bacteria, DS48-5-2<SUP>T</SUP> and DS48-6-3, were isolated from a sediment sample collected from a depth of 48 m taken from Daechung Reservoir, Republic of Korea. Comparative 16S rRNA gene sequence studies showed that the two isolates had clear affiliation with <I>Alphaproteobacteria</I> and the closest relatedness to <I>Caulobacter mirabilis</I> FWC 38<SUP>T</SUP>, <I>Caulobacter fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>Caulobacter daechungensis</I> H-E3-2<SUP>T</SUP> showing 98.5 %, 97.3 % and 97.3 % 16S rRNA gene sequence similarity, respectively, and 96.1–96.7 % similarity to all other species of the genus <I>Caulobacter</I>. The two isolates shared 100 % 16S rRNA gene sequence similarity. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (C<SUB>18 : 1</SUB>ω6<I>c</I> and/or C<SUB>18 : 1</SUB>ω7<I>c</I>), C<SUB>16 : 0</SUB>, C<SUB>18 : 0</SUB>ω7<I>c</I> 11-methyl and summed feature 3 (C<SUB>16 : 1</SUB>ω6<I>c</I> and/or C<SUB>16 : 1</SUB>ω7<I>c</I>). The G+C contents of the genomic DNA of strains DS48-5-2<SUP>T</SUP> and DS48-6-3 were 66.7 mol% and 66.2 mol%, respectively. DNA–DNA hybridization values of strains DS48-5-2<SUP>T</SUP> and DS48-6-3 with <I>C. mirabilis</I> FWC 38<SUP>T</SUP>, <I>C. fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>C. daechungensis</I> H-E3-2<SUP>T</SUP> were 19.3 %–24.4 %. Thus, based on the evidence from polyphasic studies, it is proposed that strains DS48-5-2<SUP>T</SUP> and DS48-6-3 are representatives of a novel species in the genus <I>Caulobacter</I>, for which the name <I>Caulobacter</I> <I>profunda</I> sp. nov. is proposed. The type strain is DS48-5-2<SUP>T</SUP> ( = KCTC 32480<SUP>T</SUP> = JCM 19440<SUP>T</SUP>).</P>

Asprobacter aquaticus gen. nov., sp. nov., a prosthecate alphaproteobacterium isolated from fresh water

Jin, Long,Ko, So-Ra,Lee, Chang Soo,Ahn, Chi-Yong,Oh, Hee-Mock,Lee, Hyung-Gwan Microbiology Society 2017 International journal of systematic and evolutiona Vol.67 No.11

<i>Belnapia soli</i> sp. nov., a proteobacterium isolated from grass soil

Jin, Long,Lee, Hyung-Gwan,No, Kui-Jung,Ko, So-Ra,Kim, Hee-Sik,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.6

<P>A Gram-negative, aerobic, non-motile, non-spore-forming, cocci-shaped, red-pigmented bacterium, designated strain PB-K8<SUP>T</SUP>, was isolated from grass soil sampled in Daejeon, Republic of Korea. Comparative 16S rRNA gene sequence studies showed that the isolate was clearly affiliated with the class <I>Alphaproteobacteria</I><I>,</I> and was most closely related to <I>Belnapia moabensis</I> DSM 16746<SUP>T</SUP> and <I>Belnapia rosea</I> DSM 23312<SUP>T</SUP>, showing a 16S rRNA gene sequence similarity to the type strains of each species of 98.4 and 97.2 %, respectively. The cells of strain PB-K8<SUP>T</SUP> formed red colonies on R2A agar, contained Q-9 as the predominant ubiquinone and included summed feature 3 (C<SUB>16 : 1</SUB>ω7<I>c</I> C<SUB>16 : 1</SUB>ω6<I>c</I>), C<SUB>16 : 0</SUB>, summed feature 8 (C<SUB>18 : 1</SUB>ω7<I>c</I>/C<SUB>18 : 1</SUB>ω6<I>c</I>), C<SUB>18 : 1</SUB> 2-OH and C<SUB>19 : 0</SUB> cyclo ω8<I>c</I> as the major fatty acids. The G+C content of the genomic DNA of strain PB-K8<SUP>T</SUP> was 72.1 mol%. Thus, the combined genotypic and phenotypic data supported the conclusion that strain PB-K8<SUP>T</SUP> represents a novel species of the genus <I>Belnapia</I>, for which the name <I>Belnapia soli</I> sp. nov. is proposed. The type strain is PB-K8<SUP>T</SUP> ( = KCTC 23765<SUP>T</SUP> = JCM 18033<SUP>T</SUP>).</P>