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식물(植物) 병원성(病源性) 미생물(微生物)에 미치는 Pseudomonas fluorescens, KR-164의 영향(影響)
이영환,김영일,이재평,김용웅,김용재,이재와,Rhee, Young-Hwan,Kim, Yeong-Yil,Lee, Jae-Pyeong,Kim, Yong-Wong,Kim, Yong-Jae,Lee, Jae-Wha 한국토양비료학회 1990 한국토양비료학회지 Vol.23 No.1
The antagonistic fluorescent pseudomonas, which was isolated from continuous cropping rhizosphere of pepper and cucumber, was identified as Pseudomonas fluorescens (P.f.). For further study, transformant was derived from the isolated P.f. after spontaneous mutation to give antibiotic resistance to nalidixic acid and rifampicin as marked strain. Both P.f. and transformant strains were used for this study and the results obtained were summarized as follows. 1. One of the most effective antagonistic strain, KR164, was selected against F. solani, F. oxysporum, R. solani and this strain was identified and classified as Pseudomonas fluorescens biotype IV. 2. Transformant, KR1641, was derived from strain KR164 and both strains had the same biological and biochemical characteristics. 3, Mycelial lysis and abnormal mycelia of plant pathogenic fungi were microscopically observed after simultaneous culture of fungus and given bacterial strain. 4. The length of chinese cabbage to the autolyzed became longer with given bacterial strain in dark culture. 5. Percentage of germination, number of leaves, length of height, and length of root in chinese cabbage in pot experiment were improved by inoculation of given bacterial strain. 6. The number of given bacterial strain kept generally stable until 34 days after inoculation of itself in pot experiment. Inoculation of given bacterial strain did affect the number of plant disease fungi to be decreased but did not affect the number of other bacteria, Bacillus, in pot experiment. 고추, 오이 등의 시설원예(施設園藝) 연작토양(連作土壤)에서 토양전염성(土壤傳染性) 식물병원균(植物病院菌)에 길항력(拮抗力)을 갖는 Pseudomonas fluorescens를 분리(分離), 동정(同定)하고 이 균주(菌株)를 nalidixic acid와 rifampicin에 내성을 갖는 spontaneus multant를 유도(誘導)하여 표식균주(標識菌株)를 만들었다. 이들 표식균주(標識菌株)와 모균주(母菌株)의 생화학적 특성(特性), 병원성 사장균(絲狀菌)에 대한 길항력(拮抗力), 암소배양(暗所培養), 작물(作物) pot 실험(實驗) 결과(結果)를 요약(要約)하면 다음과 같다. 1. 고추, 오이 등의 시설원예(施設園藝) 연작지(連作地) 토양(土壤)에서 형광성(螢光性) Pseudomonas를 분리(分離)하여 Rhizoctonia solani, Fusrium oxysporum, Fusarium solani에 생육(生育) 저해력(沮害力)이 우수한 KR-164를 선발(選拔)하고 이들의 분류학적(分類學的) 성질(性質)을 검토(檢討)한 결과(結果) Pseudomonas fluorescens biotype IV로 분류(分類), 동정(同定)하였다. 2. KR-164에서 표식균주(標識菌株)인 KR-1641 변이주(變異株)를 유도하였고, 이들 표식균주(標識菌株)와 모균주(母菌株)의 생화학적(生化學的) 성질(性質)은 동일(同一)하였다. 3. 분리균주(分離菌株)와 식물(植物) 병원성 사장균(絲狀菌)을 대치배양(對置培養)하여 검경한 결과(結果) 여러 형태(形態)의 균사이장(異狀)이 관찰(觀察)되었다. 4. 분리균주(分離菌株)를 배추 암소배양(暗所培養)에 처리(處理)한 결과(結果) 무처리구(無處理區)에 비해 생육기간(生育其間)이 현저하게 연장(延長) 되었다. 5. 분리균주(分離菌株)의 접종(接種)이 대조구(對照區)와 사장균(絲狀菌) 접종구(接種區)에 비해 배추의 발아율(發芽率) 및 엽수, 초장, 근장 등의 생육(生育)을 향상(向上)시켰다. 6. 토양(土壤)에 접종(接種)된 표식균주(標識菌株)는 접종(接種) 35일까지 안정(安定)하였으나 병원성 사장균(絲狀菌)은 현저하게 감소(減少)하였고, 표식균주(標識菌株)는 근권(根圈) 유용미생물(有用微生物)의 생육(生育)에 뚜렷한 영향(影響)을 미치지 않았다.
젓갈에서 분리한 Lactobacillus spp.로 제조한 요구르트의 이화학적 특성
이영환,나한주,이용규,신승이,김종현,Rhee, Young-Hwan,Na, Han-Ju,Lee, Yong-Kyu,Shin, Seung-Yee,Kim, Jong-Hyun 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.43 No.1
젓갈류로 부터 Lactobacillus cases 2주와 L. pentosus 1주를 분리, 동정한 후 이들 유산균의 요구르트 제조 Starter로서의 이용 가능성을 고찰하고자, 이들 균주를 Starter로 이용하여 요구르트를 제조한 결과 요구르트 고유의 pH값은 $4.03{\sim}4.26$, 산도는 $1.049{\sim}1.217%$, 점도는 $1,772{\sim}2,232\;cps$, 그리고 생균수는 $1.4{\times}10^9{\sim}1.6{\times}10^9\;cfu/ml$였다. 요구르트의 완충능은 (요구르트 100 ml 기준) 1.0N HCl의 경우 12.50 ml 부터 14.06 ml 소모 되었고 1.0N NaOH는 9.46 ml 부터 13.06 ml 소모 되었다. 한편, 요구르트 제조시 균주 첨가 후 24시간 부터 72시간 동안 경시적으로 ${\beta}-galactosidase$ 활성도를 조사한 결과 48시간에 최고의 효소활성을 보였으며 이후에는 감소하였다. 또한 산성조건에서 2시간 동안 경시적으로 ${\beta}-galactosidase$ 활성도와 유산균 생존율을 측정한 결과 pH 3.5에서 2시간 후에는 $20{\sim}60%$ 정도 효소활성이 감소하였으며 pH 2.5와 pH 1.5에서 효소활성은 거의 없었다. 한편, 유산균의 생존율은 pH 3.5에서 2시간 후 거의 변화가 없었으나 pH 2.5와 pH 1.5에서는 각각 $1.9{\times}10^6{\sim}1.8{\times}10^8,\;1.0{\times}10^3{\sim}2.4{\times}10^5\;cfu/ml$ 수준이었다. Three strains of lactic acid bacteria were isolated from fish and shrimp pickles. Two strains were identified as Lactobacillus casei, and one strain as L. Pentosus, respectively. All three strains were used as starters in producing yogurts. The physico-chemical characteristics of yogurts were examined. The original pH, titratable acidity, visicosity and viable cell counts of the yogurts were $4.03{\sim}4.26,\;1.049{\sim}l.217%,\;1,772{\sim}2,232\;cps\;and\;1.4{\times}10^9{\sim}1.6{\times}10^9\;cfu/ml$, respectively. In evaluating buffer capacity, $12.50{\sim}14.06\;ml$ 1.0N HCl was consumed to titrate 100 ml of yogurt to pH value 2 units below the original pH value and $9.46{\sim}13.06\;ml$ of 1.0N NaOH was consumed to pH value 4 units above the original pH value. The ${\beta}-galactosidase$ activity reached maximum at 48 hrs, and reduced gradually during fermantation. After 2 hr incubation of yogurts at $37^{\circ}C$ under different pH conditions, ${\beta}-galactosidase$ activities of three strains were reduced to 50% at pH 3.5, but there were no remaining activities neither at pH 2.5 nor at pH 1.5. Under the same pH conditions the number of viable cells decreased to $1.9{\times}10^6{\sim}1.8{\times}10^8\;cfu/ml$ at pH 2.5 and $1.0{\times}10^3{\sim}2.4{\times}10^5$ at pH 1.5, respectively. However, no significant difference was found at pH 3.5.
이영환 ( Lee Yeong Hwan ),김용범 ( Kim Yong Beom ),정도영 ( Jeong Do Yeong ),이유경 ( Lee Yu Gyeong ),김건영 ( Kim Geon Yeong ),이준승 ( Lee Jun Seung ),이중건 ( Lee Jung Geon ),최덕례 ( Choe Deog Lye ),박미자 ( Park Mi Ja ),이영 대한신장학회 2001 Kidney Research and Clinical Practice Vol.20 No.6
The incidence of Tuberculosis among the patients with end-stage renal disease(ESRD) has increased up to 16 times of that in the general population. The impairment of the cellular immunity in the ESRD patients may have a role in the pathogenesis. Extrapulmonary tuberculous manifestations such as lymph node, peritoneum, and pleura involvement are more frequent in the ESRD patients than in the general population. However, there has been no case of upper gastrointestinal(UGI) bleeding as a result of a gastric tuberculosis in the ESRD patient on hemodialysis. Here we report an unusual case of a hemodialysis patient with UGI bleeding secondary to a tuberculous gastric ulcer. A 31-year-old female on hemodialysis was admitted with melena. Endoscopy revealed a benign gastric ulcer with a visible bleeding vessel at the base, located in the anterior wall of the antrum. An exploratory laparotomy showed multiple, round, small and yellow nodules on the visceral peritoneum as well as a 1-cm sized gastric ulcer. After gastric resection, a histological examination including peritoneal nodules demonstrated chronic granulomatous inflamation with caseous necrosis and giant cells. The patient has been on antituberculosis medication and followed up in the outpatient department without any event for 8 months.
성인둔상환자에서 평균동맥압과 위해사건발생의 관련성: 단면 조사 연구
차승용 ( Seung Yong Cha ),김용환 ( Yong Hwan Kim ),홍종근 ( Chong Kun Hong ),이준호 ( Jun Ho Lee ),조광원 ( Kwang Won Cho ),황성연 ( Seong Youn Hwang ),이경렬 ( Kyoung Yul Lee ),이영환 ( Young Hwan Lee ),최성희 ( Seong Hee Choi 대한외상학회 2013 大韓外傷學會誌 Vol.26 No.2
Purpose: Non-invasive blood pressure measurement is widely used as a pre-hospital triage tool for blunt trauma patients. However, scant data exits for using the mean arterial pressure (MAP), compared to the systolic blood pressure, as a guiding index. The aim of this study was to determine the association between adverse outcomes and mean arterial pressure (MAP) and to exhibit the therapeutic range of the MAP in adult blunt trauma patients. Methods: The electronic medical records for all trauma patients in a single hospital from January 2010 to September 2012 were retrospectively reviewed. Patients below 17 years of age, patients with penetrating injuries, and patients with serious head trauma (injuries containing any skull fractures or any intracranial hemorrhages) were excluded. Adverse outcomes were defined as one of the following: death in the Emergency Department (ED), admission via operating theater, admission to the intensive care unit, transfer to another hospital for emergency surgery, or discharge as hopeless. Results: There were 14,537 patients who met entry criteria. Adverse outcomes occurred for MAPs in range from 90 to 120 mmHg. Adverse outcomes were found, after adjusting for confounding variables, to occur increasingly as the MAP declined below 90 mmHg or rose above 120 mmHg. Conclusion: Not only lower but also higher mean arterial pressure is associated with increased adverse outcomes in adult blunt trauma patients. Thus, patients with a MAP above 120 mmHg should be considered as a special group requiring higher medical attention, just as those with a MAP below 90 mmHg are.
데리다적 해체주의를 통한 플라톤의 체육사상 제고 (Ⅱ)
이영환(Lee, young-hwan),전경아(Jeon, kyung-a),안용규(Ahn, yong-kyu) 한국체육철학회 1998 움직임의철학 : 한국체육철학회지 Vol.6 No.2
Among lots of phiolsophers, Platon has been known as a philosopher, who showde big interests and opinions for Physical Education. There has been two conflicting views in Plato`s Physical Education ideas: First, Plato insists that existence about everything is idea, and this is truth of eternity. That is to say, he insist that only mental world is absolute, and it can manifest perfect existence. Accordingly, the ideology of Plato has been interpreted as the stand of dualic position of mind and body, which regarded mind and body as independent and different. So, that is taken as the most understandable approaches about Plato. Second, but if we ignore the stands of psychosomatic unit, we can`t say that we have correct stand. The deconstruction of Derrida is not consist a new flame on the existing from. That means it takes the supporting value on an unstable condition with taking around about way of the existing form. The thought of deconstruction in Derrida`s ideology doesn`t mean deconstructure. It means that we scrutinize the pedigree of an general concept which already existing. Therefore, the study about the following Plato`s idea which are discussed in terms of Derrida`s deconstruction, will open new phase of important methodical place on Physical Education philosophy