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박용서(Yong Seo Park),김병운(Byoung Woon Kim),김태춘(Tae-Choon Kim),장홍기(Hong Gi Jang),천상욱(Sang Uk Chon),조자용(Ja Yong Cho),江??(Shen Hua Jiang),허북구(Buk Gu Heo) 한국원예학회 2008 원예과학기술지 Vol.26 No.4
참다래의 생리활성 물질에 대한 기초자료 확보측면에서 2007년 11월 5일에 참다래 4품종(‘비단’, ‘대흥’, ‘제시골드’ 및 ‘헤이워드’)을 수확하여 2℃에서 30일간 저장한 다음 과피와 과육의 메탄올 추출물에 대한 생리활성 효과를 조사하였다. 총 페놀함량은 추출물이 2,000㎎・L<SUP>-1</SUP>일 때 과피와 과육 모두 ‘대흥’에서 37.6 및 17.3㎎・L<SUP>-1</SUP>로 각각 많았다. 전자공여능은 추출물의 농도가 1,000㎎・L-1일 때 과피는 ‘대흥’에서 89.3%로, 과육은 ‘비단’에서 22.3%로 가장 높았다. 총 플라보노이드 함량은 추출물의 농도가 2,000㎎・L-1일 때 과피는 ‘대흥’에서 17.1㎎・L<SUP>-1</SUP>로, 과육은 비단에서 9.0㎎・L<SUP>-1</SUP>로 가장 높았다. 아질산염 소거는 추출물의 농도가 1,000㎎・L<SUP>-1</SUP>일 때 과피는 ‘대흥’에서 80.9%, 과육은 ‘제시골드’에서 78.3%로 가장 높았다. Tyrosinase 활성 저해 효과는 추출물의 농도가 1,000㎎・L<SUP>-1</SUP>일 때 ‘제시골드’의 과육이 14.4%로 가장 높게 나타났다. 따라서 참다래는 품종 및 부위에 따라 생리활성에 차이가 있었으며, 전반적으로 아질산염 소거능이 높은 것으로 나타났다. This study was conducted to determine the physiological activities of methanol extracts from the fruit skin and flesh of Korean kiwifruit cultivars ‘Bidan’, ‘Daeheung’, ‘Jecygold’ and ‘Hayward’ which were harvested and stored at 2℃ for 30days. At the extract concentration of 2,000 ㎎・L<SUP>-1</SUP>, total phenol content was the highest in fruit skin and flesh of cv. ‘Daeheung’ by 37.6 and 17.3 ㎎・L<SUP>-1</SUP>, respectively. DPPH radical scavenging activity in 1,000 ㎎・L<SUP>-1</SUP> were very high in the fruit skin of kiwifruit cv. ‘Daeheung’ by 89.0% and in the flesh of cv. ‘Bidan’ by 22.3%. Total flavonoid content in the fruit skin of cv. ‘Daeheung’ at 2,000 ㎎・L<SUP>-1</SUP> was 17.1 ㎎・L<SUP>-1</SUP> and in the flesh of cv. ‘Bidan’ 9.0 ㎎・L<SUP>-1</SUP>. Nitrite scavenging activity in 1,000 ㎎・L<SUP>-1</SUP> showed the highest value in the fruit skin of kiwifruit cv. ‘Daeheung’ by 80.9%, and followed by the flesh of cv. ‘Jecygold’ by 78.3%. Mushroom tyrosinase inhibition activity in 1,000 ㎎・L<SUP>-1</SUP> were highest in the flesh of kiwi fruit cv. ‘Jecygold’ by 14.4%. These results indicate that Korean kiwifruits have a high nitrite radical scavenging activity, though the physiological activity of kiwifruits was affected by variety and regional parts.
박용서(Yong Seo Park),박윤점(Yun Jum Park),김현주(Hyun Ju Kim),임명희(Myung-Hee Im),이미경(Mi-Kyung Lee),김영민(Young Min Kim),조자용(Ja Yong Cho),허북구(Buk Gu Heo) 한국원예학회 2008 원예과학기술지 Vol.26 No.1
비파의 소비촉진 및 부가가치를 향상시키기 위하여 2007년6월 전남 완도군에서 재배중인 비파‘전중(田中)’을 수확하여 부위별(잎, 과육 및 씨) 에탄올 추출물의 생리활성 효과를 조사하였다. 총 페놀함량은 잎(60.6㎍·mL<SUP>-1</SUP>), 종자(22.9㎍·mL<SUP>-1</SUP>)그리고 과육(19.4㎍·mL<SUP>-1</SUP>)순으로 많았다. 추출물의 농도가 500㎎·mL<SUP>-1</SUP>일 때 부위별 전자공여능은 잎 추출물(91.8%), 종자 추출물(25.0%), 과육 추출물(14.2%)순이었다. 아질산염 소거는 추출물의 농도가 500㎎·mL<SUP>-1</SUP> 일 때 잎추출물은 73.0%, 과 육추출물은 66.9%, 종자 추출물은 64.9%를 나타내었다. Tyrosinase 활성 저해 효과는 과육, 잎,종자 추출물 순으로 높게 나타났는데,과육 추출물 1,000㎎·mL<SUP>-1</SUP>에서는 16.9%, 4,000㎎·mL<SUP>-1</SUP>에서는 33.3%의 저해율을 나타내었다. 항균활성은 잎 추출물에서 높게 나타났는데, 그 중 Streptococcus mutans 균에서 저해환의 직경이 12.1(500㎎·mL<SUP>-1</SUP>)∼15.4㎜(2,000㎎·mL<SUP>-1</SUP>)으로 크게 나타났다. This study was conducted to promote the consumption and to improve the values of the loquat (Eriobotrya japonica Lindl., cv. “Jeonjung”). We also harvested loquat grown in Wando-gun, Jeonnam Province, on June 10, 2007, and examined its physiological activities with the ethanol extracts from different plant parts. Total phenol content was high in the order of leaves (60.6 ㎍·mL<SUP>-1</SUP>), seeds (22.9 ㎍·mL<SUP>-1</SUP>) and fruits (19.4 ㎍·mL<SUP>-1</SUP>). When the concentration of extracts was 500 ㎎·mL<SUP>-1</SUP>, leaves showed highest DPPH (α,α-diphenyl-β-picryl-hydrazyl) radical scavenging activity (91.8%) and followed by seeds (25.0%) and fruits (14.2%). And nitrite scavenging activity was high in the order of leaves (73.0%), fruits (66.9%) and seeds (64.9%). Fruit extracts showed highest mushroom tyrosinase inhibition activity, especially the activity at 4,000 ㎎·mL<SUP>-1</SUP> extracts was 33.3%. The highest anti-microbial activity exhibited in the leaf extracts, especially showing the largest inhibition diameter (12.14 ㎜) against Streptococcus mutans at 500 ㎎·mL<SUP>-1</SUP> extracts.
실험적 뇌경색의 형태학적 관찰에서 Neutral Red와 Tetrazolium Salt 교차 염색의 의의
서정호,박윤관,김명현,정용구,정흥섭,서중근,이훈갑,주정화,이기찬 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.8
Serial changes in the size of infarcted area induced by MCA occlusion(MCAO) were compared with Neutral Red(NR) and 2,3,5-Triphenyl tetrazolium chloride(TTC) stains. The differences in size of the infarcted area as shown by the 2 stains and its significance were also evaluated. The experimental animals were divided into 7 groups, with each group consisting of rats; these groups were stained at 2,4,6,8,12,24 and 48 hours after MCAO. After MCAO, NR was infused into the femoral vein, after which the brain was removed, the frontal pole of the brain cut into 1.5㎜ sections, and each section photographed. Then, the NR-stained sections were immersed in TTC solution for 45 minutes and photographed. Results showed that the infarcted area progressively increased according to time duration after MCAO(one-way ANOVA, p<0.01). Between 4 and 6 hour groups, the difference of the infarcted area was greater than at any other timed groups, this being statisfically significant(unpaired t-test, p<0.05). After 6 hours, the infarcted area with NR stain became relatively stable. In contrast, however, the infracted area with TTC stain did not stabilize, but continued to increase up to 24 hours. Overall, the infarcted area with NR stain was greater than with TTC stain in all the timed groups(paired t-test, p<0.05). As time progressed, the differences tended to decrease 48 hours post occlusion. In our study, serial changes of the ischemic penumbra area were evaluated by staining the ischemic area simultaneously with Neutral red and TTC stain. The results suggest that the ischemic penumbra area may still persist even after 48 hours post-MCAO.
Seo, Kyu Won,Choi, Yong-Su,Gu, Man Bock,Kwon, Eilhann E.,Tsang, Yiu Fai,Rinklebe, Jö,rg,Park, Chanhyuk Elsevier 2017 CHEMOSPHERE - Vol.186 No.-
<P><B>Abstract</B></P> <P>A pilot-scale investigation of membrane-based aerobic digestion system dominated by endospore-forming bacteria was evaluated as one of the potential sludge treatment processes (STP). Most of the organic matter in the sludge was removed (90.1%) by the particular bacteria in the STP, which consisted of mixed liquor suspended solid (MLSS) contact reactor (MCR), MLSS oxidation reactor (MOR), and membrane bioreactor (MBR). The sludge was accumulated in the MBR without wasting, and then the effluent in STP was fed into the first step in water resource recovery facility (WRRF). According to the analysis of microbial communities in all reactors, various <I>Bacillus</I> species were present in the STP, mainly due to their intrinsic resistance to the extreme conditions. As the surviving <I>Bacillus</I> species might consume degraded microorganisms for their growth, these endospore-forming bacteria-based STP could be suitable for the sludge reduction when they operated for a long time.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A pilot-scale sludge treatment process was developed and investigated. </LI> <LI> Membrane-based aerobic digestion system could accumulate the sludge. </LI> <LI> 90% of sludge reduction was achieved in sludge treatment process. </LI> <LI> <I>Bacillus</I> species was dominant and influenced on the sludge reduction. </LI> <LI> Endospore-forming bacteria-based process could be suitable for sludge reduction. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>