Genetically predicted physical activity is associated with lower serum urate concentrations

Guan Ying,Wei Jiahe,Meng Lifeng,Li Yasong,Wang Tianle,Chen Dingwan,Qian Qilin 한국유전학회 2022 Genes & Genomics Vol.44 No.7

Background: Physical activity (PA) is considered to play an important role in the reduced gout risk. However, the epidemiology results are inconsistent and causality remains unclear. Objective: To investigate the causal relationship of PA with serum urate concentrations and gout risk by a bidirectional Mendelian randomization (MR) approach. Method: Two genome-wide association studies (GWASs) from UK Biobank were used to identify instrumental variables for self-reported moderate-to-vigorous PA (including 377,234 European individuals), accelerometer-measured 'average acceleration' PA (including 91,084 European individuals) and accelerometer-measured overall PA (including 91,105 European individuals). The summary data for serum urate (including 110,347 European individuals) and gout (including 2,115 cases and 67,259 controls) were derived from GWAS of Global Urate Genetics Consortium. Moreover, reverse direction Mendelian randomization study was conducted. The inverse-variance weighted, weighted median, Mendelian randomization Egger regression, simple mode and weighted mode and Mendelian Randomization Pleiotropy RESidual Sum and Outlier were methods we performed. Result: Genetic predisposition to accelerometer-measured 'average acceleration' PA [beta = -0.038; 95% confidence interval (CI) = -0.060,-0.015; P = 0.001] and accelerometer-measured overall PA (beta = -0.339; 95% CI = -0.522,-0.156; P = 2.8E-4) were significantly associated with decreased serum urate concentrations. Besides, there was no evidence supporting the causal association between PA and gout risk. In the reverse direction analysis, genetic predisposition to both urate and gout were not associated with PA being investigated. Conclusions: In MR study, we found that PA may reduce serum urate concentrations but not the risk of gout. Moreover, serum urate concentrations and gout were not associated with PA.

Sentinel lymph Node mapping versus systematic pelvic lymphadenectomy on the prognosis for patients with intermediate-high-risk Endometrial Cancer confined to the uterus before surgery: trial protocol for a non-inferiority randomized controlled trial (SNE

Jun Guan,Yu Xue,Rong-yu Zang,Ji-hong Liu,Jian-qing Zhu,Ying Zheng,Bo Wang,Hua-ying Wang,Xiao-jun Chen 대한부인종양학회 2021 Journal of Gynecologic Oncology Vol.32 No.4

Background: Sentinel lymph node (SLN) mapping has been recommended as an alternative staging approach to lymphadenectomy for apparent uterine-confined endometrial cancer (EC). However, the prognostic value of SLN mapping alone instead of systematic lymphadenectomy on EC patients remains unclear. Methods: A multi-center, open label, non-inferiority randomized controlled trial has been designed to identify if SLN mapping alone is not inferior to pelvic lymphadenectomy on prognosis of patients with intermediate-high-risk EC clinically confined to uterus. Eligible patients will be 1:1 randomly assigned to accept SLN mapping or pelvic lymphadenectomy. The primary endpoint is the 2-year progression-free survival (PFS). The second points are the 5-year PFS, 5-year overall survival, surgery-related adverse events and life quality. A total of 780 patients will be enrolled from 6 hospitals in China within 3-year period and followed up for 5 years. Trial Registration: ClinicalTrials.gov Identifier: NCT04276532

Secondary teachers’ conceptions of integrated writing skills: Are teachers’ conceptions aligned with the curriculum objectives

Li Guan Ying,Zhu Xinhua,Cheong Choo Mui 서울대학교 교육연구소 2020 Asia Pacific Education Review Vol.21 No.3

Integrated writing skills that emphasize integrated use of language skills and multiple source materials have attracted increasing attention in language education globally and locally in Hong Kong. This study examines teachers’ conceptions of integrated writing skills and interviewed twenty-five Chinese language teachers. Three conceptions emerged from the data, representing writing as a composite of disconnected parts (Category1), a logical inquiry (Category 2), and a developmental process (Category 3). As the categories move up, the alignment between teachers’ conceptions and the curriculum objectives increases accordingly, with the purpose of writing instruction ranging from fulfilling examination requirements, enhancing reasoning skills, to developing integrated use of language skills. The findings also reveal that although the development of integrated writing skills has been a critical component of the Chinese language curriculum since the first public examination in 2007, teachers’ receptivity toward it still varied greatly. Insufficient professional training, the legitimacy of integrated writing as a curriculum component, and the fossilization of the public examination were the factors that accounted for the differing attitudes among the teachers. The discourse of integrated writing in the Hong Kong context has been centered around high-stakes testing. The unbalanced discourse resulted in an oversimplified view that conflated the teaching and learning of integrated writing with integrated writing assessment. The study contributes to the conversation between integrated writing curriculum, instruction, and assessment. Implications for teacher professional development are discussed.

Cellular Mechanisms of a New Pyrazinone Compound that Induces Apoptosis in SKOV-3 Cells

Wang, Guan,Jiang, Meng-Ying,Meng, Ying,Song, Hong-Rui,Shi, Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

We screened a small molecular library that was designed and independently synthesized in vitro and found a new drug (MY-03-01) that is active against ovarian cancer. We established that MY-03-01 effectively inhibited SKOV-3 cell survival in a dose-dependent manner, based on cell viability rates, and that it not only induced SKOV-3 apoptosis by itself, but also did so synergistically with paclitaxel. Secondly, when MY-03-01 was applied at $40{\mu}M$, its hemolytic activity was less than 10%, compared with the control, and there was almost no damage to nor mal cells at this concentration. In addition, we used DAPI staining and flow cytometry to show that MY-03-01 could significantly induce apoptosis of SKOV-3 cells. Finally, we found that MY-03-01 likely induced SKOV-3 apoptosis by activating caspase3 and caspase9 through the mitochondrial pathway.

The subcellular distribution of MnSOD alters during sodium selenite-induced apoptosis

( Li Ying Guan ),( Qi An Jiang ),( Zhu Shi Li ),( Fang Huang ),( Yun Ren ),( Yang Yang ),( Cai Min Xu ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.6

P53 transcription-independent activity mediates selenite-induced acute promyelocytic Leukemia NB4 cell apoptosis

( Li Ying Guan ),( Fang Huang ),( Zhu Shi Li ),( Bing She Han ),( Qian Jiang ),( Yun Ren ),( Yang Yang ),( Cai Min Xu ) 생화학분자생물학회 2008 BMB Reports Vol.41 No.10

PB 상품의 재구매 의도에 영향을 미치는 요인: 지각된 심리적 위험의 조절 역할

꾸안잉 ( Guan Ying ),김효은 ( Kim Hyoeun ),김병수 ( Kim Byoungsoo ) 한국고객만족경영학회 2017 고객만족경영연구 Vol.19 No.2

최근 트레이더 조, 홀푸드 마켓, 이마트 `피코크` 등 유통업체는 품질이 우수하고 합리적 수준의 가격으로 PB 상품을 출시하고 있다. 본 연구는 소비자들의 PB 상품 재구매 의도에 미치는 요인을 살펴 보는 것을 목적으로 한다. PB 상품에 대한 고객 만족과 재구매 의도 형성의 주요 요인으로 지각된 품 질, 지각된 가치, 브랜드 신뢰를 고려하였다. 그리고 고객들이 PB 상품에 대해 지각된 심리적 위험의 조절 역할에 대해서도 살펴보았다. 본 연구는 경북 지역에 거주하는 PB 상품 구매 경험이 있는 200명의 고객 데이터를 기반으로 PLS를 사용하여 데이터를 분석하였다. 연구 모형 분석 결과 지각된 품질, 지각된 가치, 브랜드 신뢰는 고객 만족을 통해 재구매 의도에 영향을 미침을 밝혀내었다. PB 상품에 대해 위험을 낮게 느끼는 고객 집단은 지각된 가치, 지각된 품질, 브랜드 신뢰가 고객 만족을 통해 재구매 의도에 영향을 미쳤다. 하지만 PB 상품에 대해 심리적 위험을 높게 느끼는 고객 집단의 경우 지각된 가치와 지각된 품질이 브랜드 신뢰를 통해 재구매 의도에 영향을 미침을 확인하였다. 본 연구 결 과를 통해 PB 상품의 브랜드 신뢰는 재구매 의도 형성에 핵심적 역할을 담당함을 알 수 있었으며 지각된 심리적 위험의 정도가 높을 때 브랜드 신뢰의 영향이 점차 중요해짐을 확인할 수 있었다. This study explores the key antecedents of consumers` repurchase intentions about PB (private brand) products. We posited perceived quality, perceived value, and brand trust as the key components to enhance consumer satisfaction and repurchase intention. Moreover, this study investigated the moderating role of perceived psychological risk in consumers` repurchase behavior about PB products. PLS was used to analysis the hypotheses based on 200 customers. Perceived quality, perceived value, and brand trust significantly influence repurchase intention through consumer satisfaction. For customer groups with the low psychological risk, perceived quality, perceived value, and brand trust significantly affect customer satisfaction, which ultimately enhances repurchase intention. However, for customer groups with the high psychological risk, perceived quality and perceived value significantly influence brand trust, which lead to increase repurchase intention. The analysis results demonstrated the moderating role of perceived psychological risk in customer`s repurchase decisions about PB products.

The association of urinary uric acid excretion with ambulatory blood pressure values in patients with chronic kidney disease

Ying Xu,Xun Zhou,Yuqi Zheng,Haochen Guan,Chensheng Fu,Jing Xiao,Zhibin Ye 대한고혈압학회 2020 Clinical Hypertension Vol.26 No.2

Background: To analyze the association between hypertension and urinary uric acid excretion in patients with chronic kidney disease (CKD). Methods: We screened 87 patients who had been admitted at the Dept of Nephrology, Huadong hospital between April 2017 to April 2019 who had completed 24-h ambulatory blood pressure monitoring and retained 24-h urine biochemical test specimens, thirty adult patients (age ≤ 65 years) with CKD 1–2 stages were recruited in the study. Pearson’s correlation analysis and multiple linear regression analysis were used to study the correlation of urinary uric acid excretion with ambulatory blood pressure values and the association of morning mean diastolic pressure (mMDP), night mean diastolic pressure (nMDP) and CV of dMSP (coefficient of variation of day mean systolic pressure) with fractional excretion of uric acid (FEua) and uric acid clearance rate (Cur). Independent T test was used to compare the differences of blood pressure values in FEua1 (FEua< 6.0%) and FEua2 (FEua≥6.0%) or Cur1 (Cur < 6.2 ml/min/1.73 m2 ) and Cur2 (Cur ≥ 6.2 ml/min/1.73m2 ) groups according to the median of FEua or Cur, respectively. Results: After adjusting for confounding factors, multiple linear regression analysis showed that FEua was positively associated with the mMDP and nMDP, Cur was positively associated with CV of dMSP. Levels of mMDP and nMDP in FEua1 group was lower than that in FEua2 group (both P < 0.05), level of CV of dMSP in Cur2 group were higher than that in Cur1 group (P < 0.01). Conclusions: We demonstrated that there is a positive correlation of FEua with morning and night mean diastolic pressure separately and Cur is positively related to CV of dMSP in CKD population. Monitoring the trend of urinary uric acid, may have a role in the early detection for hypertension or relative risks in the population of CKD.

Clinical Significance of Axin and β-catenin Protein Expression in Primary Hepatocellular Carcinomas

Guan, Cheng-Nong,Chen, Xin-Ming,Lou, Hai-Qing,Liao, Xiang-Hui,Chen, Bao-Ying,Zhang, Pei-Weng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

The aim of the present research was to investigate clinicopathologic correlations of immunohistochemically-demonstrated axin (axis inhibition) and ${\beta}$-catenin expression in primary hepatocellular carcinomas (HCCs), in comparison with paraneoplastic, cirrhotic and normal liver tissues. Variation in Axin expression across groups were significant (P < 0.01), correlating with alpha fetoprotein (AFP), HBsAg, cancer plugs in the portal vein, and clinical stage of HCCs(P < 0.05); however, there were no links with sex, age, and tumour size (P > 0.05). Differences in cell membrane ${\beta}$-catenin expression were also statistically significant (P < 0.01), again correlated with AFP, HBsAg, cancer plugs in the portal vein, and clinical stage in HCCs (P < 0.05) but not with sex, age, and tumour size (P > 0.05). Axin expression levels in tissues with reduced membrane ${\beta}$-catenin were low (P < 0.05), also being low with nuclear ${\beta}$-catenin expression (P < 0.05). Axin and ${\beta}$-catenin may play an important role in the genesis and progression of HCC via the Wnt signal transmission pathway. Simultaneous determination of axin, ${\beta}$-catenin, AFP, and HBsAg may be useful for early diagnosis, and metastatic and clinical staging of HCCs.

DLC-1 Expression Levels in Breast Cancer Assessed by qRT-PCR are Negatively Associated with Malignancy

Guan, Cheng-Nong,Zhang, Pei-Wen,Lou, Hai-Qing,Liao, Xiang-Hui,Chen, Bao-Ying Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: The aim of this study was to explore the expression of DLC-l in breast carcinoma and any association with tumor metastasis. Methods: 51 surgical specimens of human breast carcinoma, divided into high invasive and low invasive groups according to their clinicopathological features, 30 cases of adjacent normal tissue and 28 benign breast lesions were examined by qRT-PCR for expression of DLC-1. Results: Expression level of DLC-1 in adjacent normal tissue and benign breast lesion specimens was higher than that in breast carcinoma (P<0.0001); the values in the high invasive group with synchronous metastases were also lower than in the low invasive group (P=0.0275). The correlation between DLC-1 expression level and tumor progression and metastasis of breast cancer was negative. Conclusion: As an anti-oncogene, DLC-1 could play an important part in breast carcinoma occurrence, progression, invasiveness and metastasis. Detecting the changes of the expression of DLC-1 in the breast carcinoma may contribute to earlier auxiliary diagnosis of invasiveness, metastasis and recrudescence.