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RISS 인기검색어
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- 저자 : Yin Qiming (검색결과 3 건)
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RPTOR methylation in the peripheral blood and breast cancer in the Chinese population
Yin Yifei,Lei Shuifang,Li Lixi,Yang Xiaoqin,Yin Qiming,Xu Tian,Zhou Wenjie,Li Hong,Gu Wanjian,Ma Fei,Yang Rongxi,Zhang Zhengdong 한국유전학회 2022 Genes & Genomics Vol.44 No.4
Background: Altered regulatory-associated protein of mTOR, complex 1 (RPTOR) methylation levels in peripheral blood was originally discovered as breast cancer (BC)-associated risk factor in Caucasians. Objective: To explore the relationship between RPTOR methylation and BC in the Chinese population, we conducted two independent case-control studies. Methods: Peripheral blood samples were collected from a total of 333 sporadic BC cases and 378 healthy female controls for the DNA extraction and bisulfite-specific PCR amplification. Mass spectrometry was applied to quantitatively measure the levels of methylation. The logistic regression, Spearman's rank correlation, and Non-parametric tests were used for the statistical analyses. Results: In our study, we found an association between BC and RPTOR_CpG_4 hypomethylation in the general population (per-10% of methylation, OR 1.29, P = 0.012), and a weak association between BC and RPTOR_CpG_8 hypomethylation in the women with older age (per-10% of methylation, OR 2.34, P = 0.006). We also identified age as a confounder for the change of RPTOR methylation patterns, especially at RPTOR_CpG_4, which represented differential methylation comparing age groups especially in the BC cases (age < 50 years vs age ≥ 50 years by Mann-Whitney U test, P < 0.0001 for BC cases and P = 0.079 for controls). Conclusion: Our study validated the association between hypomethylation of RPTOR and BC risk in the Chinese population also with weak effect and mostly for postmenopausal women. In addition, our findings provided novel insight for the regulation of DNA methylation upon aging or the change of hormone levels.
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Xiaoli Hu,Pinghe Yin,Ling Zhao,Qiming Yu 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1
The bloom of Phaeocystis globosa has occurredfrequently in the southern coastal areas of China in recentyears, which has led to substantial economic losses. Thisstudy investigated the effects of culture broth of strains Y1and Y4 isolated from algal blooms in Zhuhai, China onphysiological characteristics and cell viability of P. globosa. The increase in the levels of reactive oxygen species (ROS)in P. globosa cells exposed to strains Y1 and Y4 culturebroth were detected, indicating that the algal cells sufferedfrom oxidative damage. The surplus ROS induced theincrease of malondialdehyde (MDA) contents and theactivities of antioxidant enzymes, including superoxidedismutase (SOD) and catalase (CAT). The decrease inprotein content indicated that strains Y1 and Y4 culturebroth inhibited cell growth. The contents of pigmentsdecreased after 96 h treatment, indicated that oxidative stressdestroyed pigment synthesis. Furthermore, flow cytometrycoupled with the propidium iodide stain and chlorophyllauto-fluorescence was used to investigate cell viability. Results showed that chlorophyll fluorescence intensitiesand cell integrity decreased with time of exposure, whichdemonstrated that strains Y1 and Y4 culture broth couldchange membrane permeability and resulted in the loss ofthese photosynthetic pigments. The isolated strains wereidentified as Bacillus sp. by culture morphology, biochemicalreactions, and homology research based on 16S rDNA. Overall, these findings suggested that oxidative stress causedfrom Bacillus sp. potentially destroyed pigment synthesisand cell membrane integrity, and ultimately led to the lysisof the algal cells.
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Zhiqian Pang,Zhuangzhi Zhou,Dedong Yin,Qiming Lv,Lixiang Wang,Xiao Xu,Jing Wang,Xiaobing Li,Xianfeng Zhao,Guanghuai Jiang,Jinping Lan,Lihuang Zhu,Songnian Hu,Guozhen Liu 한국식물학회 2013 Journal of Plant Biology Vol.56 No.6
Plant Bowman-Birk type bran trypsin inhibitors(BBTI) belong to a family of serine protease inhibitors thatinhibit trypsin activity and play roles in plant developmentand defense responses to both biotic and abiotic stresses. Inthis study, transgenic rice plants overexpressing BBTI4 (OXBBTI4)were generated. Reverse-transcription polymerasechain reaction and western blot (WB) analysis demonstratedthat the BBTI4 mRNA and protein levels were significantlyincreased in OX-BBTI4. Notably, two BBTI4 protein formswith different molecular weight (18 kD and 28 kD) wererevealed by WB analysis. In non-transgenic plants, BBTI4-28kD and BBTI4-18kD were mainly expressed in roots andleaves, respectively, while in transgenic OX-BBTI4 plants,both protein forms were expressed constitutively. Subcellularanalysis revealed that BBTI4 is localized in the cytosol. Moreover, Xanthomonas oryzae pv. oryzae (Xoo) inoculationexperiments demonstrated that transgenic OX-BBTI4 riceplants conferred partial but broad-spectrum Xoo resistance. InOX-BBTI4 transgenic rice plants, the expression of OsPR3 andOsPR10a proteins was induced and gradually increased afterXoo infection, while the expression of OsPR1a, OsPR1b andOsPR-pha remained unchanged. Taken together, these resultssuggest that BBTI4 may play a role in rice resistance to Xoo,and OsPR3 and OsPR10a may be involved in the OX-BBTI4-dependent partial Xoo resistance response.
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