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A Duplex PCR Assay for Rapid Detection of Phytophthora nicotianae and Thielaviopsis basicola
Liu, Na,Jiang, Shijun,Feng, Songli,Shang, Wenyan,Xing, Guozhen,Qiu, Rui,Li, Chengjun,Li, Shujun,Zheng, Wenming The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.2
A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens Phytophthora nicotianae and Thielaviopsis basicola. The specific primers for P. nicotianae were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, ras gene and hgd gene, while the specific primers for T. basicola were designed based on its ITS regions and ${\beta}$-tubulin gene. The specificity of the primers was determined using isolates of P. nicotianae, T. basicola and control samples. The results showed that the target pathogens could be detected from diseased tobacco plants by a combination of the specific primers. The sensitivity limitation was $100fg/{\mu}l$ of pure genomic DNA of the pathogens. This new assay can be applied to screen out target pathogens rapidly and reliably in one PCR and will be an important tool for the identification and precise early prediction of these two destructive diseases of tobacco.
A Duplex PCR Assay for Rapid Detection of Phytophthora nicotianae and Thielaviopsis basicola
Na Liu,Shijun Jiang,Songli Feng,Wenyan Shang,Guozhen Xing,Rui Qiu,Chengjun Li,Shujun Li,Wenming Zheng 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.2
A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens Phytophthora nicotianae and Thielaviopsis basicola. The specific primers for P. nicotianae were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, ras gene and hgd gene, while the specific primers for T. basicola were designed based on its ITS regions and β-tubulin gene. The specificity of the primers was determined using isolates of P. nicotianae, T. basicola and control samples. The results showed that the target pathogens could be detected from diseased tobacco plants by a combination of the specific primers. The sensitivity limitation was 100 fg/μl of pure genomic DNA of the pathogens. This new assay can be applied to screen out target pathogens rapidly and reliably in one PCR and will be an important tool for the identification and precise early prediction of these two destructive diseases of tobacco.
Robust Secure Transmit Design with Artificial Noise in the Presence of Multiple Eavesdroppers
( Xiaochen Liu ),( Yuanyuan Gao ),( Nan Sha ),( Guozhen Zang ),( Shijie. Wang ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.6
This paper studies secure wireless transmission from a multi-antenna transmitter to a single-antenna intended receiver overheard by multiple eavesdroppers with considering the imperfect channel state information (CSI) of wiretap channel. To enhance security of communication link, the artificial noise (AN) is generated at transmitter. We first design the robust joint optimal beamforming of secret signal and AN to minimize transmit power with constraints of security quality of service (QoS), i.e., minimum allowable signal-to-interference-and-noise ratio (SINR) at receiver and maximum tolerable SINR at eavesdroppers. The formulated design problem is shown to be nonconvex and we transfer it into linear matrix inequalities (LMIs). The semidefinite relaxation (SDR) technique is used and the approximated method is proved to solve the original problem exactly. To verify the robustness and tightness of proposed beamforming, we also provide a method to calculate the worst-case SINR at eavesdroppers for a designed transmit scheme using semidefinite programming (SDP). Additionally, the secrecy rate maximization is explored for fixed total transmit power. To tackle the nonconvexity of original formulation, we develop an iterative approach employing sequential parametric convex approximation (SPCA). The simulation results illustrate that the proposed robust transmit schemes can effectively improve the transmit performance.
Yining Mu,Tuo Zhang,Tianqi Chen,Fanqi Tang,Jikai Yang,Chunyang Liu,Zhangxiaoxiong Chen,Yiming Zhao,Peng Du,Haibo Fan,Yan Zhu,Guozhen Liu,Ping Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2020 NANO Vol.15 No.03
In recent years, all inorganic bismuth lead-halide perovskite nanocrystals [CsPbX3 (X=Cl, Br, I)] have received extensive attention due to their high performance in fluorescence quantum yield, narrow emission spectrum, and adjustable emission range. However, the disadvantages of high cost and poor stability have greatly limited the development prospects of the material. Here, in order to develop a perovskite quantum dot lasing cavity with high chemical stability, high quality factor and low fabrication cost, we have successfully fabricated a 3D random cavity device based on porous silicon/TiO2 nanowires. A TiO2 nanowire is grown on the porous silicon to form a 3D resonant cavity, and a perovskite quantum dot is spin-coated on the surface of the 3D resonant cavity to form a novel 3D complex film. The novel structure enhances the chemical stability and lasing quality factor of the resonant cavity while the fluorescence generated by the large quantum dots in the spatial interference structure constitutes the feedback loop, which will provide favorable support for the development of information optics.
Zhiqian Pang,Zhuangzhi Zhou,Dedong Yin,Qiming Lv,Lixiang Wang,Xiao Xu,Jing Wang,Xiaobing Li,Xianfeng Zhao,Guanghuai Jiang,Jinping Lan,Lihuang Zhu,Songnian Hu,Guozhen Liu 한국식물학회 2013 Journal of Plant Biology Vol.56 No.6
Plant Bowman-Birk type bran trypsin inhibitors(BBTI) belong to a family of serine protease inhibitors thatinhibit trypsin activity and play roles in plant developmentand defense responses to both biotic and abiotic stresses. Inthis study, transgenic rice plants overexpressing BBTI4 (OXBBTI4)were generated. Reverse-transcription polymerasechain reaction and western blot (WB) analysis demonstratedthat the BBTI4 mRNA and protein levels were significantlyincreased in OX-BBTI4. Notably, two BBTI4 protein formswith different molecular weight (18 kD and 28 kD) wererevealed by WB analysis. In non-transgenic plants, BBTI4-28kD and BBTI4-18kD were mainly expressed in roots andleaves, respectively, while in transgenic OX-BBTI4 plants,both protein forms were expressed constitutively. Subcellularanalysis revealed that BBTI4 is localized in the cytosol. Moreover, Xanthomonas oryzae pv. oryzae (Xoo) inoculationexperiments demonstrated that transgenic OX-BBTI4 riceplants conferred partial but broad-spectrum Xoo resistance. InOX-BBTI4 transgenic rice plants, the expression of OsPR3 andOsPR10a proteins was induced and gradually increased afterXoo infection, while the expression of OsPR1a, OsPR1b andOsPR-pha remained unchanged. Taken together, these resultssuggest that BBTI4 may play a role in rice resistance to Xoo,and OsPR3 and OsPR10a may be involved in the OX-BBTI4-dependent partial Xoo resistance response.