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Guan Yi-Xin,Pan Hai-Xue,Gao Yong-Gui,Yao Shan-Jing,Cho Man-Gi The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2
A size exclusion chromatography (SEC) process, in the presence of denaturant in the refolding buffer was developed to refold recombinant human $interferon-\gamma$ ($rhIFN-\gamma$) at a high concentration. The $rhlFN-\gamma$ was overexpressed in E. coli resulting in the formation of inactive inclusion bodies (IBs). The IBs were first solubilized in 8 M urea as the denaturant, and then the refolding process performed by decreasing the urea concentration on the SEC column to suppress protein aggregation. The effects of the urea concentration, protein loading mode and column height during the refolding step were investigated. The combination of the buffer-exchange effect of SEC and a moderate urea concentration in the refolding buffer resulted in an efficient route for producing correctly folded $rhIFN-\gamma$, with protein recovery of $67.1\%$ and specific activity up to $1.2\times10^7\;IU/mg$.
Carbon coated porous nickel phosphides nanoplates for highly efficient oxygen evolution reaction
Yu, Xin-Yao,Feng, Yi,Guan, Buyuan,(David) Lou, Xiong Wen,Paik, Ungyu The Royal Society of Chemistry 2016 Energy & environmental science Vol.9 No.4
<P>Electrochemical splitting of water provides an attractive way to produce hydrogen fuel. Unfortunately, the efficient and large-scale H-2 production is still hindered by the sluggish kinetics of the oxygen evolution reaction (OER) at the anode side of a water electrolyzer. Starting from metal-organic frameworks (MOFs), we demonstrate a template-engaged strategy to transformNi-Ni Prussian blue analogue (PBA) nanoplates into porous carbon coated nickel phosphides nanoplates with mixed phases of Ni5P4 and Ni2P. For comparison, NiO and Ni(OH)(2) porous nanoplates with the similar morphology have also been synthesized from the same precursor. Benefitting from their structural merits and the in situ formed catalytically active oxidized nickel species, the as-derived nickel phosphides manifest excellent electrocatalytic activity for OER superior to NiO and Ni(OH)(2).</P>
Zhou Liu,Yi Gu,Xin Li,Yanyan Liu,Ying Ye,Shihe Guan,Jiabin Li 대한진단검사의학회 2019 Annals of Laboratory Medicine Vol.39 No.2
Background: Carbapenem-resistant hypervirulent (hypermucoviscous) Klebsiella pneumoniae (CR-HMKP) poses a significant public health challenge. We investigated its epidemiology and molecular characteristics in a tertiary care hospital in eastern China. Methods: CR-HMKP were identified among 106 non-duplicated carbapenem-resistant K. pneumoniae isolates (from June 2013 to September 2017) using the string test. The pulsotype (PT) and sequence type (ST) of CR-HMKP isolates were determined using pulsed-field gel electrophoresis and multilocus sequence typing. Resistance determinants, capsular serotypes, and virulence genes were detected by PCR and sequencing. Representative isolates from each PT were selected, and their virulence phenotypes were established using the serum killing and Galleria mellonella lethality assays. Results: Of the 106 isolates, 13 (12.3%) were CR-HMKP. Seven were positive for blaNDM-1 and shared the same genotype (PT5/ST1764); the others were positive for blaKPC-2, belonged to ST11, and were divided into four different PTs. The serotype of all blaNDM-1-positive isolates was K64, while that of blaKPC-2-positive isolates were K47 (N=4) and K64 (N=2). The NDM-1-producing HMKP isolates were positive for aerobactin, exhibited high serum resistance, and elicited significantly increased larval mortality compared with the other isolates. All patients had received invasive treatment prior to infection by NDM-1-producing HMKP. The infections occurred between July and August 2016 and were hospital-acquired. Conclusions: NDM-1-producing HMKP ST1764 isolates were identified; this is the first report worldwide on an outbreak of nosocomial infection caused by these isolates. Effective surveillance and strict infection control strategies should be implemented to prevent CR-HMKP dissemination.
Adsorption Performance of Proteins to CM Sepharose FF and DEAE Sepharose FF Adsorbents
Yao, Shan-Jing,Guan, Yi-Xin,Yu, Li-Hua 한국화학공학회 2003 Korean Journal of Chemical Engineering Vol.20 No.1
Adsorption equilibrium and kinetic were studied for the binding of proteins to CM Sepharose FF and DEAE Sepharose FF. The influence of temperature pH, viscosity, initial concentration and the volume of adsorbents on the adsorption characteristics was investigated in detail. The results showed that the isotherms of lysozyme to CM Sepharose FF were well described by a Langmuir-type correlation. The two phase resistance model describing the dynamic adsorption process of lysozyme, papain, BSA to CM Sepharose FF was presented, and the pore diffusion coefficients were determined by using this model and the dynamic adsorption data.
Magnolol-Induced H460 Cells Death via Autophagy but Not Apoptosis
Hai-bo Li,Xin Yi,Jian-mei Gao,Xi-xiang Ying,Hong-quan Guan,Jian-chun Li 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.12
We have reported that the protective effect of Magnolol on TBHP-induced injury in human nonsmall lung cancer H460 cells is partially via a p53 dependent mechanism. In this study, we found that Magnolol displayed a stimulatory effect at low concentrations (≤20 µM) whilst inhibitory effect at high concentrations (≥40 µM) in H460 cells. To investigate the mechanism of inducing the biphasic effect in H460 cells with Magnolol, we showed that Magnolol stimulated DNA synthesis at low concentrations and displayed an inhibition effect at high concentrations in H460 cells. More importantly, the inhibition of DNA synthesis was accompanied by the S phase cell cycle arrest and the appearance of intense intracytoplasmic vacuoles. These vacuoles can be labeled by autophagic marker monodansylcadaverin (MDC), 3-methyladenine (3- MA), an inhibitor of autophagy, was able to inhibit the occurrence of autophagy. The results of the LDH activity assay and TUNEL assay also showed that Magnolol at high concentrations inhibiting H460 cell growth was not via apoptotic pathway. Furthermore, accompanied by the occurrence of autophagy, the expression of phospho-Akt was down-regulated but PTEN significantly was up-regulated. In conclusion, Magnolol induces H460 cells death by autophagy but not apoptotic pathway. Blockade of PI3K/PTEN/Akt pathway is maybe related to Magnololinduced autophagy. Autophagic cells death induction by Magnolol underlines the potential utility of its induction as a new cancer treatment modality.
Na Chen,Dan Li,Xin‑Yi Wang,Zhen‑Jie Guan,Jian‑Tang Jiang,Kang‑Jun Wang 대한금속·재료학회 2022 ELECTRONIC MATERIALS LETTERS Vol.18 No.4
Magnetic composites have received increasing attention for electromagnetic wave absorption (EMA) applications. However,the practical EMA performance of the materials is severely hampered by mismatching impedance characteristics and finiteelectromagnetic attenuation capacity. Controlling the components and building the architecture fabrication is necessary tosolve these issues. Herein, a series of Fe3O4,Fe3O4&Fe and Fe microspheres with flower-like hierarchical structures wereconstructed through a solvothermal method followed by an annealed process. This hierarchical structure and the synergyeffect of dielectric dissipation and magnetic loss capacity offer Fe3O4a perfect impedance matching, providing an excellentEMA performance of an effective absorption bandwidth (EAB) of 4.0 GHz and a reflection loss (RL) of 67.9 dB. Meanwhile,the coordination of the hierarchical structures and the multiple components endow Fe3O4&Fe composites with an EAB aswide as 5.7 GHz (9.0–14.7 GHz) and a RL as strong as 78.7 dB at 1.88 mm, which covers 75% X and 45% Ku bands. Sucha remarkable lightweight and broad properties is due to the decent X band impedance matching and appropriate attenuationcapacity. Therefore, this work highlights the significant of regulating the hierarchical structure and components to enhancethe EMA performances.
Magnolol-Induced H460 Cells Death via Autophagy but Not Apoptosis
Li, Hai-Bo,Yi, Xin,Gao, Jian-Mei,Ying, Xi-Xiang,Guan, Hong-Quan,Li, Jian-Chun 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.12
We have reported that the protective effect of Magnolol on TBHP-induced injury in human non-small lung cancer H460 cells is partially via a p53 dependent mechanism. In this study, we found that Magnolol displayed a stimulatory effect at low concentrations $(\leq20{\mu}M)$ whilst inhibitory effect at high concentrations $(\geq20{\mu}M)$ in H460 cells. To investigate the mechanism of inducing the biphasic effect in H460 cells with Magnolol, we showed that Magnolol stimulated DNA synthesis at low concentrations and displayed an inhibition effect at high concentrations in H460 cells. More importantly, the inhibition of DNA synthesis was accompanied by the S phase cell cycle arrest and the appearance of intense intracytoplasmic vacuoles. These vacuoles can be labeled by autophagic marker monodansylcadaverin (MDC), 3-methyladenine (3-MA), an inhibitor of autophagy, was able to inhibit the occurrence of autophagy. The results of the LDH activity assay and TUNEL assay also showed that Magnolol at high concentrations inhibiting H460 cell growth was not via apoptotic pathway. Furthermore, accompanied by the occurrence of autophagy, the expression of phospho-Akt was down-regulated but PTEN significantly was up-regulated. In conclusion, Magnolol induces H460 cells death by autophagy but not apoptotic pathway. Blockade of PI3K/PTEN/Akt pathway is maybe related to Magnolol-induced autophagy. Autophagic cells death induction by Magnolol underlines the potential utility of its induction as a new cancer treatment modality.
Shan-Jing Yao,Yi-Xin Guan,Hai-Xue Pan,Yong-Gui Gao,Man-Gi Cho 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2
A size exclusion chromatography (SEC) process, in the presence of denaturant in the refolding buffer was developed to refold recombinant human interferon- (rhIFN-) at a high concentration. The rhIFN- was overexpressed in E. coli, resulting in the formation of inactive inclusion bodies (IBs). The IBs were first solubilized in 8 M urea as the denaturant, and then the refolding process performed by decreasing the urea concentration on the SEC column to suppress protein aggregation. The effects of the urea concentration, protein loading mode and column height during the refolding step were investigated. The combination of the buffer-exchange effect of SEC and a moderate urea concentration in the refolding buffer resulted in an efficient route for producing correctly folded rhIFN-, with protein recovery of 67.1% and specific activity up to 1.2 107 IU/mg.