Depletion of BubR1 promotes premature centrosomal localization of cyclin B1 and accelerates mitotic entry.

Park, Sun-Yi,Kim, Sujeong,Cho, Hyeseon,Kwon, Soon-Hwan,Chae, Sunyoung,Kang, Dongmin,Seong, Yeon-Sun,Cho, Hyeseong Landes Bioscience 2009 Cell cycle Vol.8 No.11

<P>The role of BubR1 has been established mainly in mitosis as an essential mitotic checkpoint protein although it is expressed throughout the cell cycle. To explore a possible role of BubR1 in regulating the G(2) phase of cell cycle, we have employed siRNA-mediated hBubR1 knockdown in HeLa cells. Here, we demonstrate that reducing BubR1 levels during the G(2) phase causes accelerated mitotic entry. As expected, BubR1 depletion leads to degradation of cyclin B(1) in the G(2) phase. Intriguingly, cyclin B(1) is prematurely targeted to centrosomes appearing at early G(2) phase in BubR1-depleted cells despite its low levels. This is in contrast to control cells where cyclin B(1) appears at the centrosomes in early prophase based on cell cycle-specific localization of CENP-F. Furthermore, cyclin B/Cdk1 kinase activity in early G(2) is aberrantly high in BubR1-depleted cells. Together, our results indicate that hBubR1 depletion triggers premature centrosomal localization of cyclin B(1) probably leading to premature mitotic entry. This study is the first to suggest a role of hBubR1 in controlling centrosome targeting of cyclin B(1) and timing of mitotic entry.</P>

IGCC 용 유동층 고온건식탈황공정에서 Zinc-Titanate 탈황제의 100 시간 연속운전 성능실험

이창근,조성호,권학선,김근범,채현규,진경태,손재익 한국화학공학회 2002 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.40 No.2

벤치규모의 고온건식탈황 유동층공정의 연속운전을 탈황제의 장기 반응성, 내마모성, 공정의 신뢰성을 확보하기 위하여 수행하였다. 분무건조법에 의하여 제조된 zinc-titanate 탈황제를 사용하였으며, 반응기 압력은 5기압, 황화반응온도 600℃, 재생반응온도 700℃로 유지하였고, 두 유동층 반응기 사이의 탈황제의 고체순환은 안정적이었다. 환원성 연료 가스중에 7,700 ppm의 H_2S가 황화반응기를 통과한 후 가스중의 황화합물 배출농도를 100시간동안 40ppm미만으로 연속적으로 유지하였다. 두 유동층 반응기에서의 반응과 비산유출 특성을 밝히기 위하여 입자의 밀도, 황성분, SEM, 수은기공분석, BET, 입도분포 등의 분석이 이루어졌다. Continuous operation was performed at high pressure and high temperature to see the long-term reactivity and the attrition resistance of the sorbent, the reliability of the process in a bench-scale KIER fluidized hot gas desulfurization process. A spray dried sorbent consisting of zinc-oxide and titanium oxide was used. The pressure was maintained at 5 atm, the temperatures were 600℃ in a desulfurizer and 700℃ in the the regenerator. Solids were circulated continuously and stably between two fluidized reactors at high pressure. The H_2S concentration at exit was consistently maintained for 100 hours below 40 ppmv for a simulated coal gas containing 7,700 ppm H_2S. The analysis of solid samples, such as densities, sulfur content, SEM, Mercury pore analysis, BET, size distribution, were contributed to uncover the characteristics of reactions and entrainment in two fluidized bed reactors

경기도지역 학교급식시설 실태분석

이보숙,양일선,한경수,채인숙 한국조리과학회 1997 한국식품조리과학회지 Vol.13 No.2

Conventional foodservice system has been the most prevalent system across the country. However, all the elementary students throughout the country is not being provided with school lunch program because of the restriction on equipping school foodservice facilities. So commissary foodservice system was introduced to school lunch program in 1991. This study was designed to evaluate condition of existing major equipment including volume, probable useful life, and frequency of use and determine equipment improvements on conventional and commissary school foodservice systems. A questionnaire was developed and mailed to 87 dietians of conventional and 46 dietians of commissary school foodservices in Kyunggi-do. Response rates were 89.7% and 91.3%, respectively. Approximately one third of commissary foodservices were converted from conventional to commissary. Only 52.6% of conventional and 53.5% of commissary foodservice facilities/equipments have been desirable in quality and quantity. Capacity, funcitional ability, using frequency, and relationship with efficient work management were analyzed according to school foodservice system. Content analysis was conducted regarding to dietitians opinions on foodservice facilities/equipment.

Hyaline Cartilage Regeneration Using Mixed Human Chondrocytes and Transforming Growth Factor-β₁- Producing Chondrocytes

Song, Sun U.,Cha, Young-Deog,Han, Jeoung-Uk,Oh, In-Suk,Choi, Kyoung Baek,Yi, Youngsuk,Hyun, Jong-Pil,Lee, Hyeon-Youl,Chi, Guang Fan,Lim, Chae-Lyul,Ganjei, J. Kelly,Noh, Moon-Jong,Kim, Seong-Jin,Lee, D Mary Ann Liebert, Inc 2005 Tissue engineering Vol.11 No.9-10

<P>The purpose of this study was to investigate the efficacy of cartilage regeneration when using a mixture of transforming growth factor-beta1 (TGF-beta1)-producing human chondrocytes (hChon-TGF-beta1) and primary human chondrocytes (hChon) ('mixed cells'), compared with either hChon-TGF-beta1 or hChon cells alone. Specifically, mixed cells or hChon cells were first injected intradermally into the backs of immune-deficient nude mice to test the feasibility of cartilage formation in vivo. Both the mixed cells and the hChon-TGF-beta1 cells alone induced cartilage formation in nude mice, whereas hChon cells alone did not. To further test the efficacy of the cells in generating cartilage, an artificially induced partial thickness defect of the femoral condyle of a rabbit knee joint was loaded with hChon-TGF-beta1 cells with or without mixing additional untransduced hChon cells, and hyaline cartilage regeneration was observed at 4 or 6 weeks. The efficiency of complete filling of the defect and the quality of tissue generated after implanting were evaluated on the basis of a histological grading system modified from O'Driscoll et al. (J. Bone Joint Surg. 70A, 595, 1988). Significantly, mixed cells (14.2 +/- 0.9) produced significantly better results than hChon-TGF-beta1 (9.0 +/- 1.7) or hChon (8.0 +/- 1.8) cells alone. Histological and immunohistochemical staining of the newly repaired tissues produced after treatment with either mixed cells or hChon-TGF-beta1 cells alone showed hyaline cartilage- like characteristics. These results suggest that the implantation of mixed cells may be a clinically efficient method of regenerating hyaline articular cartilage.</P>

오염된 인삼으로부터 분리된 Citrobacter sp.에 대한 인삼사포닌의 생육억제 효과

박채규(Chae-Kyu Park),곽이성(Yi-Seong Kwak),홍순기(Soon-Gi Hong),이훈상(Hoon-Sang Lee),황미선(Mi-Sun Hwang),이만휘(Man-Hee Rhee),원준연(Jun-Yeon Won),한경호(Gyeong-Ho Han) 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.3

오염된 인삼분말로부터 1종의 세균을 순수분리하여 API kit 및 전자현미경을 이용하여 형태적, 생리적 특성을 조사하였다. 분리균은 직경 약 1.0 ㎛이었고 길이는 2.0~6.0 ㎛ 로 세포표면에 규칙적으로 분포되어 있는 편모로 이동하는 간균이었다. 분리균은 β-galactosdase, arginine dihydrolase, ornithin decarboxylase를 가지고 있으며 탄소원으로 citrate 를 이용할 수 없었고, H2S는 생성하지 못하였다. 그러나 glucose, manitol, sorbitol, rhamnose, arabinose 등의 당 및 amygdalin을 탄소원으로 이용할 수 있었다. 이상의 API kit를 이용한 생리적 특성분석 및 전자현미경을 이용한 미세형태적 특성 관찰결과에 의해 본 균주는 장내세균과 (Enterobacteriaceae)에 속하는 Citrobacter sp.로 동정되었다. 한편 분리균주 Citrobacter sp.의 생육에 미치는 인삼사포닌의 영향을 조사한 결과 사포닌은 농도의존적으로 균의 생육을 억제하는 경향을 나타내었다. 즉, 사포닌을 0.05, 0.5, 2.0, 4.0% 첨가하고 38oC에서 3 일 동안 배양한 후 사포닌 비첨가군과 비교한 결과 균의 상대적 생육저해율은 각각 28.6, 66.7, 92.4, 97.7%로 나타났다. 사포닌의 미생물 생육 억제작용은 비교적 고농도인 4.0% 사포닌 첨가시에도 초기의 6.2×10?에서 1.0×10? CFU/g으로 완만한 증가경향을 나타내어 사멸작용보다는 정균작용일 것으로 추측된다. A bacterium isolated from contaminated white ginseng was identified using API kit and electron microscope. This isolate was determined as rod shaped bacterium having about 1.0 μm in diameter and 2.0 to 6.0 ㎛ in length. It had motility by peritrichous flagellum. The isolate had β-galactosidase, arginine dihydrolase and ornithin decarboxylase. It did not have ability not only to use citrate as sole carbon source and but also to produce H2S. However, it could ferment glucose, manitol, sorbitol, rhamnose, arabinose and amygdalin. From these obserbations, the isolate was identified as Citrobacter sp. Ginseng saponin was added to culture of Citrobacter sp. in order to investigate saponin's influence on its growth. The strain was incubated at 38℃ for 3 days after addition of 0.05, 0.5, 2.0 and 4.0% (w/v) of saponin, respectively and the growth rates was investigated. The relative bacterial growth inhibition rates showed 28.6, 66.7, 92.4 and 97.7%, respectively, when compared with saponin non-treated group. These results suggest that the growth of Citrobacter sp. is inhibited by saponin in a concentration-dependent manner.

건강기능식품에서 인삼제품 현황

박채규(Chae-Kyu Park),곽이성(Yi-Seong Kwak),황미선(Mi-Sun Hwang),김석창(Seok-Chang Kim),도재호(Jae-Ho Do) 한국식품과학회 2007 식품과학과 산업 Vol.40 No.2

TCDD (2,3,7,8-tetrachlorodibenzodioxin)로 급성독성을 유도한 자성 기니픽의 대퇴골 무게감소에 대한 홍삼사포닌의 방어효과

곽이성(Yi-Seong Kwak),경종수(Jong-Soo Kyung),송용범(Yong-Bum Song),위재준(Jae-Joon Wee),박종대(Jong-Dae Park),김시관(Si-Kwan Kim),황미선(Mi-Sun Hwang),김석창(Seok-Chang Kim),박채규(Chae-Kyu Park),도재호(Jae-Ho Do) 고려인삼학회 2006 Journal of Ginseng Research Vol.30 No.3

본 연구는 TCDD로 급성독성을 유도한 자성 기니픽의 대퇴골 무게감소에 대한 홍삼사포닌의 효과를 조사하기 위해 수행하였다. 48 마리의 자성기니픽 (820±25 g)을 6 군으로 나누어 정상군 (NC), TCDD 단독투여군 (TT), 사포닌 전투여군 (PE10, 20), 사포닌후투여군 (CE10, 20)으로 나누었다. NC군은 vehicle과 saline 만을 투여하였고, TT군은 TCDD를 단회투여 (5.0 ㎍/㎏, i.p) 하였다. PE10 및 20군은 TCDD 투여 1 주일 전부터 총 3 주동안 사포닌을 투여 (복강) 하였다. 반면 CE10 및 20군은 TCDD 투여한 직후부터 총 3 주동안 사포닌을 투여 (복강) 하였다. 사포닌 투여군의 체중변화를 살펴보면 TT군은 TCDD 투여후 유의적인 체중감소 현상이 관찰되었으나 PE10, 20 및 CE10, 20 등 사포닌 투여군에서는 TT군에 비해 체중이 모두 증가하는 경향을 보였다. TCDD 투여는 자성기니픽의 대퇴골 무게를 유의적으로 감소시키는 반면 홍삼사포닌의 투여는 대퇴골 무게감소를 억제하는 경향을 보였다. 대퇴골 무게감소 억제현상은 PE20군에서 가장 높게 나타났다. 대퇴골의 Ca²? 도 TT군은 NC군 대비 약 20.4% 감소하는데 반해 사포닌 투여군은 TT군 대비 Ca²? 함량이 증가되는 경향을 나타내었다. 이러한 결과로부터 홍삼사포닌은 TCDD에 의해 억제된 기니픽의 대퇴골내 Ca²? 함량을 증가시킴으로써 대퇴골의 무게감소를 회복시키는 것으로 추론할 수 있겠다. To investigate the protective effect of saponin from red ginseng extract, 2,3,7,8-tetrachlorodibenzo--dioxin (TCDD) was exposed to female guinea pigs and then femur weights was measured. Forty eight female guinea pigs (820±25 g) were divided into 6 groups. Normal control group (NC) received vehicle and saline; only TCDD-treated group (TT) received TCDD (5.0 ㎍/㎏, single dose) intraperitoneally; pretreated group of saponin 10 (PE 10) received 10 ㎎/㎏ of saponin i.p. for 4 weeks from 1 week before TCDD-exposure; pretreated group of saponin 20 (PE 20) also received 20 ㎎/㎏ of saponin i.p. for 4 weeks from 1 week before TCDD-exposure. While, post-treated group of saponin 10 (CE 10) received 10 ㎎/㎏ of saponin i.p for 3 weeks after TCDD-exposure. Post-treated group of saponin 20 (CE 20) received 20 ㎎/㎏ saponin i.p for 3 weeks after TCDD-exposure. Body weight of TT group was significantly decreased after TCDD-exposure. However, body weight in all saponin-treated gropus increased throughout the experimental period, although the increasing rate was slower than that of NC group. Body weights of PE 10 and 20 groups showed more higher increase than those of CE groups during the experimental period. Decrease of femur weights in female guinea pigs by TCDD intoxification was significantly recovered by the saponin treatment. Decrease of Ca²? level of femurs in female guinea pigs exposed TCDD also recovered by the treatment of saponin from red ginseng extract. Especially, PE20 group showed the highest increase of the Ca²? level in femur among the saponin treated groups. These results suggest that ginseng saponin might be a useful protective agent against femur damage caused to decrease of Ca²? by TCDD.

4-[(N-Imidazol-2-ylmethyl)anilino]pyranopyridine Analogs as Novel Anti-Angiogenic Agents

Lee, Sun-Kyung,Chae, Sun-Mi,Yi, Kyu-Yang,Kim, Nak-Jeong,Oh, Chang-Ho Korean Chemical Society 2005 Bulletin of the Korean Chemical Society Vol.26 No.4

We attempted to replace a benzopyran ring of 4-[(N-imidazol-2-ylmethyl)-4-chloroanilino]benzopyran, previously discovered as anti-angiogenic agent with antitumor activity, with pyranopyridines. The [3,2-c]-, [3,2-b]-, [2,3-c]-, and [2,3-b]-pyranopyridines with -(imidazol-2-ylmethyl)aniline moiety at the 4-position, were synthesized respectively, and evaluated for primary anti-angiogenic properties through primary cultured HUVEC tube formation assay. From this study, we found that the pyranopyridine ring, especially [3,2-b]- and [2,3-c]-isomer, can replace the benzopyran ring of the compound 1 and can be optimized through the introduction of substituents both on the pyranopyridine ring and the aniline moiety for the identification of a novel anti-angiogenic agent.

인공둥지(Artificial Wood Box)의 입구크기에 따른 번식조류의 경쟁구조 분석

노선호 ( Sun-ho No ),고준서 ( Jun-seo Go ),신세영 ( Se-young Shin ),이정은 ( Jeong-eun Lee ),최한이 ( Han-yi Choi ),채주리 ( Ju-ri Chae ),신규철 ( Gyu-cheol Shin ),유영한 ( Young-han You ) 한국환경생태학회 2016 한국환경생태학회 학술대회지 Vol.2016 No.2

둥지의 입구크기에 따라 번식조류의 경쟁구조를 확인하고자 2015년에서 2016년까지 조류의 번식기간(4-7월) 동안에 주 2회 인공둥지를 모니터링 하였다. 인공둥지는 2015년과 2016년 2월에 충청남도 공주시, 금산시, 부여군, 충청북도 영동군, 옥천군 일대에 총 60개를 설치하였으며, 각각입구지름 40㎜(가로150×세로150×높이250㎜) 12개, 70㎜(가로250×세로250×높이350㎜) 12개, 80㎜(가로250×세로250×높이350㎜) 12개, 90㎜(가로250×세로250×높이350㎜) 12개, 입구지름 100㎜(300×300×400㎜) 12개를 설치하였다. 연구기간 동안 인공둥지에서 번식한 조류는 참새(Passer montanus), 박새(Parus major), 곤줄박이(Parus varius), 딱새(Phoenicurus auroreus auroreus), 파랑새(Eurystomus orientalis), 소쩍새(Otus sunia), 원앙(Aix galericulata)총 7종이였다. 입구의 크기 별로 살펴보면 지름40㎜에서 참새, 박새, 곤줄박이, 70㎜에서 참새, 박새, 곤줄박이, 파랑새, 소쩍새, 80㎜에서 박새, 곤줄박이, 딱새, 90m에서 곤줄박이, 딱새, 100㎜에서 박새, 곤줄박이, 소쩍새, 원앙이 각각 번식하였다. 번식실패요인은 다양하게 확인되었다. 주요 번식실패요인으로, 박새는 곤줄박이와 원앙, 소쩍새에 의한 둥지강탈, 곤줄박이는 원앙에 의한 둥지강탈, 소쩍새는 미부화, 원앙은 둥지포기와 미부화가 있었다. 결과적으로, 인공둥지에서 번식하는 조류의 생태적 지위는 원앙이 가장 높았으며, 크기가 상대적으로 작은 조류는 입구가 큰 둥지를 선택하였을 때 상대적으로 큰 조류에게 둥지를 강탈당했다. 따라서 나무구멍에서 번식하는 조류에 있어서 입구의 크기를 달리 제작한 인공둥지를 설치함으로 조류의 종간 경쟁을 최소화 하며, 번식성공률을 높일 수 있는 대체 산란지를 제공할 수 있을 것으로 판단된다.

Textural Properties of Processed Foods Produced from Newly Developed Non-Glutinous Rice Cultivars

Mi-Sun Ha,Yi-Woo Roh,Kwon-Pyo Hong,Yoon-Suk Kang,Dong-Chae Jung,Kwang-Ho Kim,Sang-Kyu Park,Sang-Do Ha,Dong-Ho Bae 한국식품과학회 2007 Food Science and Biotechnology Vol.16 No.5

This study was undertaken to investigate the producibility of processed foods utilizing 6 newly developed nonglutinous rice cultivars. First, cooked rice, cake, cookies, bread, and slender rice cake sticks were prepared with the newly developed cultivars; then their physicochemical and textural properties were evaluated. The rice samples had similar pasting temperatures and peak times, but different viscosities and other pasting properties. The textural analysis results suggested that ‘Chucheong’ was appropriate for cooked rice due to its low amylose content, hardness, and springiness; ‘Ilphumbyeo’ for rice cakes due to its high amylose content, moderate cohesiveness and adhesiveness, and low hardness; ‘Ilphumbyeo’ for cookies due to its high amount of protein, and low cohesiveness and adhesiveness; ‘Ilphumbyeo’ for bread due to its high amylose content, moderate hardness, and low consistency; and ‘Ilphumbyeo’ for the slender rice cake sticks due to its low hardness, moderate breakdown, paste viscosity, and setback.