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Jeong, Jiyun,Lee, Yeolin,Yoo, Yeongeun,Lee, Myung Kyu Elsevier 2018 Colloids and surfaces. B, Biointerfaces Vol.162 No.-
<P><B>Abstract</B></P> <P>Agarose gel can be used for three dimensional (3D) cell culture because it prevents cell attachment. The dried agarose film coated on a culture plate also protected cell attachment and allowed 3D growth of cancer cells. We developed an efficient method for agarose film coating on an oxygen-plasma treated micropost polystyrene chip prepared by an injection molding process. The agarose film was modified to maleimide or Ni-NTA groups for covalent or cleavable attachment of photoactivatable Fc-specific antibody binding proteins (PFcBPs) via their N-terminal cysteine residues or 6xHis tag, respectively. The antibodies photocrosslinked onto the PFcBP-modified chips specifically captured the target cells without nonspecific binding, and the captured cells grew 3D modes on the chips. The captured cells on the cleavable antibody-modified chips were easily recovered by treatment of commercial trypsin-EDTA solution. Under fluidic conditions using an antibody-modified micropost chip, the cells were mainly captured on the micropost walls of the chip rather than on the bottom of it. The presented method will also be applicable for immobilization of oriented antibodies on various microfluidic chips with different structures.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We developed a method for agarose film coating on a 3D micropost chip. </LI> <LI> Covalent or cleavable antibody-modified chip was fabricated by film modification. </LI> <LI> Cells were specifically and efficiently captured on the antibody-modified chips. </LI> <LI> Cells captured on the cleavable chips were recovered by trypsin-EDTA treatment. </LI> <LI> Cells were predominantly captured on the micropost walls under fluidic conditions. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Seo, Jongsu,Tsvetkov, Nikolai,Jeong, Seung Jin,Yoo, Yeongeun,Ji, Sanghoon,Kim, Jeong Hwan,Kang, Jeung Ku,Jung, WooChul American Chemical Society 2020 ACS APPLIED MATERIALS & INTERFACES Vol.12 No.4
<P>Solid oxide fuel cells produce electricity directly by oxidizing methane, which is the most attractive natural gas fuel, and metal nanocatalysts are a promising means of overcoming the poor catalytic activity of conventional ceramic electrodes. However, the lack of thermal and chemical stability of nanocatalysts is a major bottleneck in the effort to ensure the lifetime of metal-decorated electrodes for methane oxidation. Here, for the first time, this issue is addressed by encapsulating metal nanoparticles with gas-permeable inorganic shells. Pt particles approximately 10 nm in size are dispersed on the surface of a porous La<SUB>0.75</SUB>Sr<SUB>0.25</SUB>Cr<SUB>0.5</SUB>Mn<SUB>0.5</SUB>O<SUB>3</SUB> (LSCM) electrode via wet infiltration and are then coated with an ultrathin Al<SUB>2</SUB>O<SUB>3</SUB> layer via atomic layer deposition. The Al<SUB>2</SUB>O<SUB>3</SUB> overcoat, despite being an insulator, significantly enhances the immunity to carbon coking and provides high activity for the electrochemical oxidation of methane, thereby reducing the reaction impedance of the Pt-decorated electrode by more than 2 orders of magnitude and making the electrode activity of the Pt-decorated sample at 650 °C comparable with those reported at 800 °C for pristine LSCM electrodes. These observations provide a new perspective on strategies to lower the operation temperature, which has long been a challenge related to hydrocarbon-fueled solid oxide fuel cells.</P> [FIG OMISSION]</BR>
Engineering <i>Pseudomonas putida</i> KT2440 to convert 2,3-butanediol to mevalonate
Yang, Jeongmo,Im, Yeongeun,Kim, Tae Hwan,Lee, Myeong Jun,Cho, Sukhyeong,Na, Jeong-geol,Lee, Jinwon,Oh, Byung-keun Elsevier 2020 Enzyme and microbial technology Vol.132 No.-
<P><B>Abstract</B></P> <P>Biological production of 2,3-butanediol (2,3-BDO), a C4 platform chemical, has been studied recently, but the high cost of separation and purification before chemical conversion is substantial. To overcome this obstacle, we have conducted a study to convert 2,3-BDO to mevalonate, a terpenoid intermediate, using recombinant <I>Pseudomonas putida</I> and this biological process won’t need the separation and purification process of 2,3-BDO. The production of mevalonate when 2,3-BDO was used as a substrate was 6.61 and 8.44 times higher than when glucose and glycerol were used as substrates under the same conditions, respectively. Lower aeration contributed to higher yields of mevalonate in otherwise identical conditions. The maximum mevalonate production on the shaking flask scale was about 2.21 g/L, in this study (product yield was 0.295, 27% of theoretical yield (1.10)). This study was the first successful attempt for mevalonate production by <I>P. putida</I> using 2,3-BDO as the sole carbon source and presented a new metabolic engineering tool and biological process for mevalonate synthesis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Pseudomonas putida</I> KT2440 can metabolize 2,3-butanediol as a sole carbon source. </LI> <LI> 2,3-butandiol was converted to mevalonate by engineered <I>P. putida</I> KT2440 successfully. </LI> <LI> <I>atoB</I> gene expression and aeration optimization enhanced the mevalonate production. </LI> </UL> </P>