생약재 첨가 사료를 투여한 넙치(Paralichthys olivaceus)의 비특이적 면역반응, 혈액성분 및 항병력 효과

정승희,이주석,한형균,전창영,이해영 한국어병학회 2002 한국어병학회지 Vol.15 No.1

본 연구는 식물성 생약재의 열수추출이 농도별로 첨가된 사료를 넙치(Paralichthys olivaceus)에 경구 투여한 다음 비특이적 면역반응 및 질병 저항성의 향상여부를 알아보고자 하였다. 실험에는 약쑥과 삼지구엽초를 7:3(w/w)의 비율로 열추출한 후, 0%, 0.1%, 0.5% 및 1%의 농도로 첨가하여 만든 2종류 혼합생약재(2-혼합구)와 약쑥, 삼지구엽초, 연교, 구절초, 박하, 지유, 삼백초, 상백피, 팔각의 9종류 생약재가 동일한 농도로 열수추출한 후 제작한 9종류 혼합생약재(9-혼합구)를 사용하였다. 이들 농도별 첨가사료를 넙치(평균 10.3±2.5g)에 12주 동안 경구 투여하면서 혈청내 라이소자임 활성, 혈청의 Escherichia coli에 ??살균능력 및 혈액학적 변화를 조사하였다. 그리고 최종 투여 12주 후에 넙치 에드와드병의 원인균 Edwardsiella tarda의 생균 공격실험을 실시하여 상대생존율(RPS)로써 저항성 향상효과를 살펴보았다. 2-혼합구 0.1%는 비특이적 면역활성을 크게 증가시켰으며, 상대생존율이 67%로 높게 나타나 가장 효과적인 질병 저항성 향상효과를 보였다. 따라서 2-혼합구 0.1%는 넙치 체내 비특이적인 면역기구를 자극함으로써 어체의 자연저향성을 증강시켜 세균성 질병의 감염에 대한 방어력을 상당히 향상시킬 수 있을 것으로 사료된다. 9-혼합구 0.1% 및 0.5%는 비록 비특이적 면역활성은 증가시키지 못하였으나, 상대생존율은 각각 53%를 나타내어 질병 저항성을 일부 향상시키는 효과가 있었다. Effects of medicinal herb extract on nonspecific immune responses, hematology and disease resistance against Edwardsiella tarda in olive flounder, Paralichthys olivaceus were evaluated. Wormwood, Artemisia asiatica NAKAI and barrenwort, Epimedium koreanum NAKAI were mixed at a ratio of 7 : 3 (w/w) for 2-herbs extract and wormwood, barrenwort, Korean forsythia, Forsythia koreana NAKAI, chrysanthemum, Chrysanthemum zawadskii var. latilobum KITAMURA, peppermint, Mentha arvensis L. var, piperascens MALINV., great burnet, Snaguisorba afficinalis L., Lizard tail. Saururus chinensis BAILL., mulberry, Morus alba L., and star anise, Illicium varum HOOK, f, at the same weight for 9-herbs extract. Two-herbs of 9-herbs extract were prepared by heating after adding 10㎖ of distilled water per g of the herb mixtures. Fish (10.3±2.5g) were fed the experimental diets supplemented with the 2-herbs or 9-herbs extract at the different concentrations of 0%, 0.1%, 0.5% and 1% per kg diet for 12 weeks. Lysozyme and bactericidal activities of serum, and hematological characteristics were examined during experimental period. After feeding test period, all experimental groups were challenged with E. tarda. Lysozyme activity from the fish fed the diet supplemented with 0.1% or 0.5% of 2-herbs extract was significantly higher than the control. But there was no difference both in bactericidal activity and hematology among each group. Sixty seven % of relative percent survival values (RPS) in the group fed the diet supplemented with 0.1% of 2-herbs was higher than the other group and the control. These results suggest that supplenmentation of 0.1% of 2-herbs extract to a commercial diet may enhance disease resistance in olive flounder. Although both 0.1% and 0.5% 9-herbs extract did not improve non-specific immune reponses, they could enhance disease resistance of 53% RPS, respectively.

폐농양 및 복부농양을 일으킨 Eikenella corrodens 감염 2예 : Two Case Reports and Review

정용희,류미숙,김영식,이우인,이희주,서환조 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.1

Eikenella corrodens is a slowly growing, facultative anaerobic gram-negative bacillus. It is a normal inhabitant of the human upper respiratory tract, and it is recognized as an infrequent cause of invasive disease. To date, a few cases of E. corrodens infection were noted from all other organ. We report herein two cases of E. corrodens infection. In one case E. corrodens was isolated in the lung abscess and in the other case it was in the appendiceal abscess. The patients were treated empirically with penicillin G. The results of the bacterial culture and sensitivity test were available with it. The patients were discharged 15 days later with marked improvement after treatment. Our report clearly demonstrates that E. corrodens can be the pathogen of lung abscess and intraabdominal abscess. We reviewed descriptions from the literature of infections caused by E. corrodens.

Biogas Production from Agricultural Wastes and Residues in Tropical Region

Joo, Yeong-Hee,Jeon, Yong-Woon,Calilung, Edwin J.,Elepano, Arnold R. 한국토양비료학회 1985 한국토양비료학회지 Vol.18 No.4

농산폐유기물(農産廢有機物)을 원료(原料)로한 메탄가스시설(施設)을 Philippine소재(所在) 국제미작연구소(國際米作硏究所)에 설치(設置)하여 가스발생량(發生量 ) 시험(試驗)을 한 결과(結果)는 다음과 같다. 1. 원료(原料) 자원별(資源別) 메탄가스발생량조사(發生量調査) 가. 볏짚, 왕겨, 옥수수줄기 및 야자수열매껍질은 분요혼합(糞尿混合)으로 가스발생량(發生量)이 증대되었음. 나. 야자수열매껍질을 1주일(週日) 호기발효(好氣醱酵)시킨후(後) 메탄발효원(醱酵源)으로 활용(活用)한 결과(結果)는 무처리(無處理)에 비(比)하여 가스발생량(發生量)이 증대되었음. 다. 볏짚을 메탄발효원료(醱酵原料)로 활용(活用)한 결과(結果) 전처리(前處理)한 볏짚에 비(比)해 가스발생량(發生量)이 증대되었으며 발생기간(發生期間)도 연장(延長)되었음. 라. 질소함량(窒素含量)이 높은 Azolla는 분뇨대신(糞尿代身) 메탄발효원(醱酵源)으로 활용(活用)이 가능(可能)하였음. 마. 메탄발효액(醱酵液)의 pH 6이하(以下)에서는 가스발생(發生)이 어려웠음. 2. 취사용(炊事用) 매탄가스시설(施設) 가스발생기간(發生期間) 연장시험(延長試驗) 가. 볏짚괴(塊)(Compact straw)분뇨혼합처리(糞尿混合處理)는 볏짚 (Loose straw)분뇨혼합처리(糞尿混合處理)에 비(比)하여 원료(原料) 투입(投入) 및 제거작업(除去作業)이 용이(容易)하였으나 가스발생량(發生量)이 다소(多少)낮았음. 나. 볏짚분뇨(糞尿)를 혼합(混合)하여 메탄발효(醱酵)시킨 결과(結果), 6인가족(人家族) 취사이용(炊事利用)이 가능(可能)한 가스는 70일(日) 발생(發生)되었음. 다. 발효조(醱酵槽)를 지하(地下)에 매설(埋設)하고 그늘시설(施設)을 한 결과(結果) 발효액온(醱酵液溫)이 일정(一定)하게 유지(維持)되어 가스발생량(發生量)이 증대되고 발전기간(發全期間)도 연장(延長)되었음. 3 경제성분석(經濟性分析) 가. 3,600Kcal로 환산한 메탄가스 생산비용(生産費用)은 0.14불(弗)/$m^3$로 같은 열량(熱量)인 LPG 0.54불(弗)/kg, 석유(石油) 0.32불(弗)/kg보다 낮았음. 나. 메탄발효(醱酵)로 유기질비료생산(有機質肥料生産), 공해방지(公害防止) 및 가스사용(使用)이 편리(便利)한 점(點)을 감안한다면 메탄가스는 타연료(他燃料)에 비(比)해 가장 유효한 대체(代替)에너지임. Biogas production from agricultural wastes were summarized as follows: 1. Biogas Generation Characteristics of Various Manures and Residues a. Gas yield from crop residues like rice straw, rice hull, corn stalk and coconut husk can be improved by addition of animal manures. b. Gas yield from coconut husk can be improved through aerobic fermentation for at least one week before loading in the digester. c. Gas yield from fresh rice straw is better than from pre-fermented one, whether alone or in combination with animal manures. d. Initial study has shown that fresh azolla can be substituted for animal manures in manurerice straw combinations and gas yield derived based on unit volatile solids loaded is actually better than for manure-residue combinations. e. Gas production is highly sensitive to substrate pH and becomes almost nil at a pH of below 6. 2. Effect of ambient conditions and other factors on biogas production in a house hold-size digester. a. Results showed that compaction of rice straw in straw-manure combination can reduce gas yield compared with loosely mixed straw. b. The effective gas production period extended to 70 days using freshly threshed rice straw and fresh cattle manure as feed material. c. Underground and above ground digesters with shade have relatively more stable substrate temperature than aboveground exposed digesters. This relative temperature instability may likely be the reason for lower gas yield for the exposed aboveground digester loaded with loose straw-cattle manure substrate, compared with the underground digester with the same substrate. 3. Economic Analysis a. Based on prevailing costs of fuel, materials, and labor in the Philippines, biogas produced from the household size system is cheaper than either LPG or kerosene. b. If other benefits like organic fertilizer, pollution control and convenience are considered, biogas will surely be the best alternative fuel source.

Development of Swine Waste-water Treatment System for A Small Farm

Yeong-Hee Joo(朱永熙),Ryo Tatsukawa(立川 凉) 한국토양비료학회 1993 한국토양비료학회지 Vol.26 No.2

美國의 農業硏究動向 分析에 關한 硏究

Yeong-Hee Joo,Yang-Hee Cho,Yoo-Seob Kim 한국국제농업개발학회 1994 韓國國際農業開發學會誌 Vol.6 No.2

日本의 國際農業技術協力動向

Yeong-Hee Joo,Joon-Kook Kwon,An-Cheol Chang 한국국제농업개발학회 1996 韓國國際農業開發學會誌 Vol.8 No.3

總獨過의 農薰硏究 • 指導體樞 統合

Yeong-Hee Joo,Young-Sang Kim 한국국제농업개발학회 1996 韓國國際農業開發學會誌 Vol.8 No.2

이스라엘의 農業擔觀과 硏究動向

Yeong-Hee Joo,Yang-Hee Cho,Joon-Kook Kwon 한국국제농업개발학회 1993 韓國國際農業開發學會誌 Vol.5 No.1

和開의 農業構造와 硏究動向

Yeong-Hee Joo,Yang-Hee Cho,Joon-Kook Kwon 한국국제농업개발학회 1992 韓國國際農業開發學會誌 Vol.4 No.2

氣候變化가 作物生産에 미치는 影響

Yeong-Hee Joo 한국국제농업개발학회 1994 韓國國際農業開發學會誌 Vol.6 No.3