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Hwang, Ji-Hyeon,Shin, Yong-Keol,Park, So-Yeon,Kim, Jeesoo,Kim, Su-Mi,Kim, Byounghan,Park, Jong-Hyeon,Lee, Jong-Soo,Lee, Kwang-Nyeong American Society for Microbiology 2016 Journal of clinical microbiology Vol.54 No.1
<P>During an outbreak of foot-and-mouth disease (FMD), real-time reverse transcription-PCR (rRT-PCR) is the most commonly used diagnostic method to detect viral RNA. However, while this assay is often conducted during the outbreak period, there is an inevitable risk of carryover contamination. This study shows that the carryover contamination can be prevented by the use of target-specific restriction endonuclease in that assay.</P>
식물공장 내 광주기 및 광도가 흰민들레의 생육과 수량에 미치는 영향
황연현(Yeon Hyeon Hwang),박지은(Ji Eun Park),장영호(Young Ho Chang),안재욱(Jae Uk An),윤혜숙(Hae Suk Yoon),홍광표(Kwang Pyo Hong) (사)한국생물환경조절학회 2016 생물환경조절학회지 Vol.25 No.4
본 연구는 완전 인공광형 식물공장 내에서 RBW LED(red:blue:white = 2:1:1)의 광주기와 광도가 흰민들레생육에 미치는 영향을 구명하고자 수행하였다. 완전 인공광형 식물공장에서 각각 광주기 3수준과 광도 4수준으로 설정하였고, 분무수경으로 광주기 실험은 270일, 광도 실험은 120일 동안 재배하였다. 광주기별 전 재배기간 동안 8회 수확한 1주당 수확 총엽수는 16/8시간 처리구에서 주당 224매로 12/12시간 처리구의 220매와 비슷하게 많았고 8/16시간 처리구에서 151매로 가장 적어 대체로 명기가 길수록 수확엽수도 많아지는 경향이었다. 지상부 생체중은 16/8h 처리구에서 125g으로 가장 높았고 다음으로 12/12h 처리구 91g, 8/16h 처리구 56g 순이었다. 광도별전 재배기간 동안 총 4회 수확한 엽수는 150μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> 처리구에서 123매로 가장 많았으며 다음으로 107매인 200μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> 처리구와 95매인 100μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> 처리구이었고, 50μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> 처리구에서 56매로 가장 적었다. 1주당 지상부 총 생체중은 150μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> 처리구에서 43.6g으로 가장 높았고 다음으로 100μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP>과 200μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> 처리구로써 각각 34.6g과 32.2g이었으며 50μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> 처리구에서 18.2g으로 가장 낮았다. 이상의 결과 흰민들레 최적 생장을 위한 완전 인공광형 식물공장의 RBW LED(red:blue:white = 2:1:1) 조명의 광조건은 광주기 16/8h, 광도 150μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP>이었다. The objective of this study was to examine the effect of photoperiod and light intensity of RBW LED (red:blue:white = 2:1:1) on the growth of Taraxacum coreanum Nakai in a fully artificial light type plant factory. 3 photoperiods and 4 light intensity were used respectively in a fully artificial light type plant production system. Plants were cultured with three photoperiods and four light intensity regimes (conditions) for 270 and 120 days, respectively, using nutrient film technique (NFT) or aeroponics culture methods. For each photoperiod, the total leaves per plant harvested 8 times in all cultivation period was 224 in the 16/8(day/light) photoperiod that had no significant difference from 220 in the 12/12 photoperiod and the lowest number of leaves was 151 occurred in the 8/16 photoperiod, which means that the longer photoperiod, the more leaves harvest. Total fresh weight of above ground was the high in order of in 16/8 photoperiod as 125g, 12/12 photoperiod as 91g, 8/16 photoperiod as 56g. For each light intensity, the total leaves per plant harvested 4 times in all cultivation period was the great in order of 150μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> as 123, 200μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> as 107, 100μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> as 95, 50μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> as 56 which was the smallest number of total leaves harvest. Total fresh weight of above ground per plant was the high in order of 150μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> as 43.6g, 100μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> as 34.6g, 200μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> as 32.2g, 50μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> as 18.2g. From these results, it was concluded that photoperiod of 16/8 and light intensity at 150μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP> can be used as the light condition of RBW LED (red:blue:white = 2:1:1) for optimal growth of Taraxacum coreanum Nakai in a fully artificial light type plant factory.