Adipocyte PHLPP2 inhibition prevents obesity-induced fatty liver

Yelin Jeong,Ah-Reum Oh,Young Hoon Jung,Sang Bae Lee,KyeongJin Kim 한국실험동물학회 2022 한국실험동물학회 학술발표대회 논문집 Vol.2022 No.7

Targeting E3 ubiquitin ligases and their adaptors as a therapeutic strategy for metabolic diseases

Jeong Yelin,Oh Ah-Reum,Jung Young Hoon,Gi HyunJoon,Kim Young Un,Kim KyeongJin 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Posttranslational modification of proteins via ubiquitination determines their activation, translocation, dysregulation, or degradation. This process targets a large number of cellular proteins, affecting all biological pathways involved in the cell cycle, development, growth, and differentiation. Thus, aberrant regulation of ubiquitination is likely associated with several diseases, including various types of metabolic diseases. Among the ubiquitin enzymes, E3 ubiquitin ligases are regarded as the most influential ubiquitin enzymes due to their ability to selectively bind and recruit target substrates for ubiquitination. Continued research on the regulatory mechanisms of E3 ligases and their adaptors in metabolic diseases will further stimulate the discovery of new targets and accelerate the development of therapeutic options for metabolic diseases. In this review, based on recent discoveries, we summarize new insights into the roles of E3 ubiquitin ligases and their adaptors in the pathogenesis of metabolic diseases by highlighting recent evidence obtained in both human and animal model studies.

Tight Junction Assembly Ensures Maintenance of Pregnancy during Embryogenesis in a Mouse Model

Yelin Jeong,Inchul Choi 한국동물생명공학회(구 한국동물번식학회) 2019 Journal of Animal Reproduction and Biotechnology Vol.34 No.4

Recent studies showed that tight junctions (TJs) integrity and assembly are required for blastocyst development in mouse and pig models. However, the biological functions of TJs associated with embryo implantation and maintenance of pregnancy were not investigated yet. To examine whether disrupted TJs affect further embryo development, we employed RNAi approach and inhibitor treatment. The embryos were injected with Cxadr (Coxsackievirus and adenovirus receptor) siRNA for knock down (KD) and treated with Adam10 (A Disintegrin and Metalloproteinase specific inhibitor 10; GI254023X; SI). We compared blastocyst development and paracellular sealing assay using FITC dextran uptake between control and KD or SI embryos. Finally, we transferred control and Cxadr KD or Adam 10 SI treated blastocyst to uteri of recipients. Cxadr KD and Adam 10 SI showed lower blastocyst development and more permeable to FITC-dextran. Moreover, we observed that half of KD and inhibited embryos failed to maintain pregnancies after the second trimester. Our findings suggested that TJs integrity is required for the maintenance of pregnancy and can be used as a selective marker for the successful application of assisted reproduction technologies.

Tight Junction Integrity Is Required for Successful Pregnancy Maintenance

Yelin Jeong,Inchul Choi 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06

Understanding preimplantation embryo development is important because of early embryonic mortality during pre-and post-implantation in assisted reproductive technology (ART). Thus, assessment of in vitro produced embryo prior to ET is the most final in vitro step to monior embryo development and select the best embryo. However, diagnosis of early embryo developmental potential in ART is still limited. Tight junction (TJ) is a crtitical component for blastocyst formation including a fluid-filled cavity, but the functional role was not extensively elucidated after cavitation. To elucidated the importance, we employed loss of function studies and chemical treatment for disruption on interaction between TJs. In the knock-down (KD) or inhibition treated embryos (IHB), half of embryos were arrested between morula to blastocyst transition period. Moreover, among KD and IHB blastocyst transferred to uterus of recipients, half of them failed to maintain pregnancies after 2nd trimester. Collectively, TJ complex and its integrity provide insights into implication of translational research for prediction and selection of developmental competency in human ART.

성격 유형에 따른 한국어 학습자의 학습 유형 연구

정예린 ( Yelin Jeong ),원미진 ( Mijin Won ) 한국심리유형학회 2021 심리유형과 인간발달 Vol.22 No.1

본 연구는 한국어 학습자의 성격 유형 및 학습 유형의 분포와 양상을 살피고 성격 유형과 학습 유형의 관계를 분석하는 데에 목적이 있다. 이를 위해 한국의 대학교 언어 교육 기관 학생들과 국내의 대학이나 대학원에 재학 중인 한국어 학습자 275명을 대상으로 설문조사를 실시하였다. 학습유형 선호도를 알기 위한 설문조사는 Willing(1988)의 ‘How do you learn best?’라는 학습 유형진단 도구를 사용하였으며, 통계 분석 결과는 의사소통적 학습 유형> 구체적 학습 유형 > 권위적 학습 유형> 분석적 학습 유형 순으로 나타났다. 성격 유형은 MBTI(Myers-Briggs Type Indicator)를 이용하여 분류한 뒤 4쌍의 지표별로 특정한 학습 유형을 선호하는 것이 유의한 차이가 있는지 연구하였다. 또한, 성격 이외에 개별 변인(성별, 연령, 한국어 학습 기간, 한국 거주 기간, 학습자 숙달도, 문화권)이 학습 유형에 영향을 미치는지 살펴보았다. 성격 유형에 따른 학습 유형에 대한 차이는 두 가지 하위 차원에서 유의한 결과를 나타내어, 직관형 학습자가 감각형 학습자보다 구체적 학습 유형을 더 선호하는 것을, 판단형 학습자가 인식형 학습자보다 분석적 학습 유형을 더 선호하는 것을 확인할 수 있었다. 특히 분석적 학습 유형은 성격, 거주 기간, 문화권 모두에서 유의한 차이를 나타내어 학습자 변인의 영향을 많이 받는 학습 유형임을 알게 한다. 또한, 동·서양 문화권에 따라 학습자를 분류하여 통계 분석하였을 때, 판단형의 학습자가 압도적으로 많은 동양권에서 분석적 학습 유형을 더 선호한다는 유의한 차이가 발견되어, 앞서 판단형(J)-인식형(P) 지표와 분석적 학습 유형 간 유의미한 결과를 지지하였고 학습 유형과 성격 유형 간의 연관성을 좀 더 뚜렷하게 밝힐 수 있었다. 본 연구는 MBTI 성격 유형 분류를 통해 한국어 학습자들이 선호하는 학습유형과 효과적인 교수 방법까지 예측 가능하다는 결과를 이끌어 낼 수 있다는 의의를 가지며 이를 통해 다양한 배경의 학습자를 가진 한국어 교실에서 학습자의 성격 유형과 학습 유형을 활용하여 효과적인 교육 방안을 계획할 수 있을 것으로 기대한다. This study aims to examine the distribution and aspects of personality types and learning types of Korean learners and to analyze the relationship between personality types and learning types. To this end, students from university language institutions in Korea and 275 Korean language learners attending universities or graduate schools in Korea were surveyed, and the SPSS/win Version 25.0 program was used to analyze the collected data. The survey to know learning type preferences used Willing (1988)'s Learning Type survey called 'How do you learn best?' and statistical analysis results were presented in order of Communicative > Specific > Authoritative > Analytical Learning Type. We study whether there is a significant difference in the preference of a specific type of learning for each of the four indicators after classifying personality types using the Myers-Briggs Type Indicator (MBTI). In addition to personality, individual variables (gender, age, learning period in Korean, period of residence in Korea, learner proficiency, and culture) were analyzed to affect the type of learning. Differences in learning types by personality type showed significant results on two sub-dimensions, indicating that intuitive learners prefer specific learning types to sensory learners, and judgmental learners prefer analytical learning types to perceived learners. In particular, analytical learning types represent significant differences in both personality, duration of residence, and culture, making us aware that they are highly influenced by learner variables. In addition, when classifying learners according to Eastern and Western cultures, significant differences were found that judgmental learners preferred analytical learning types in an overwhelming number of Eastern regions, which earlier supported meaningful results between judgmental (J) and analytical learning types. This study means that MBTI personality type classification can lead to predictable learning types and effective teaching methods preferred by Korean learners, and expects to use them in Korean classrooms with diverse backgrounds to plan effective education methods.

국외 교원양성기관의 진로교육 교육과정 운영 사례와 함의

정진철(Jinchul Jeong),류지은(Ji-eun Ryu),서예린(Yelin Suh) 한국진로교육학회 2019 진로교육연구 Vol.32 No.1

학교 현장에서 진로교육이 강조되고 있는 현재, 진로교육의 활성화를 위해서는 진로전담교사를 포함한 모든 교사들이 진로교육의 필요성을 인식하고, 진로교육을 실시할 수 있는 역량을 갖출 필요가 있다. 교사들의 진로교육 관련 전문성 확보를 위한 방안으로는 현직 교사 대상의 진로교육 역량 강화를 위한 연수와 함께 교원양성과정에서의 예비교원 대상의 진로교육을 고려해 볼 수 있을 것이다. 교원양성과정에서 진로교육 비중을 확대할 경우, 장기적 관점에서 모든 교사가 진로교육을 이해하고 관련 기초지식과 기초역량을 갖추는 데 기여할 수 있기 때문이다. 이를 위해 이 연구에서는 일본, 프랑스, 핀란드, 오스트리아 등 4개 국가의 국외 교원양성기관의 진로교육 교육과정 운영 사례를 분석하고, 이를 종합하여 국내 교원양성기관의 진로교육 운영 강화에 필요한 시사점을 제시하였다. In order to activate career education, it is necessary for all teachers including career dedicated teachers to recognize the necessity of career education and to have the capacity to carry out career education. In order to secure the professionalism related to career education of teachers, it is possible to consider career education for preliminary teachers in the process of teacher training as well as training for strengthening career education capacity for current teachers. If the proportion of career education is expanded in the course of teacher training, all teachers can contribute to understanding career education, basic knowledge and basic competency in the long term. For this purpose, this study analyzed cases of career education curriculum of foreign teacher education institutes in four countries such as Japan, France, Finland and Austria, and suggested implications for strengthening career education of domestic teacher education institutions.

서울시 교육주체의 미래농업에 대한 교육수요 분석

정진철(Jinchul Jeong),서예린(YeLin Suh),강민지(MinJi Kang),조홍용(HongYong Jo) 학습자중심교과교육학회 2022 학습자중심교과교육연구 Vol.22 No.15

목적 이 연구의 목적은 미래농업 유망분야를 도출하여 중등단계에서 교육의 필요성이 인정되는지 확인하고, 서울시 소재 중학교에 소속된 학생 및 학부모, 교원을 대상으로 도출된 미래농업 유망분야에 대한 교육수요를 확인하고자 함에 있다. 방법 이 연구의 목적을 달성하기 위해 먼저, 관련 선행연구 고찰과 함께 1차 전문가자문을 실시하여 미래농업 유망분야를 도출하였으며, 이후 도출된 분야에 대해 2차 전문가 자문을 실시하여 미래 농업에서의 중요성, 인력의 충분성, 고등학교 단계에서의 인력양성의 필요성 지표를 통해 중등단계 교육 필요성을 검토하였다. 다음으로 중학생 및 학부모, 교원을 대상으로 미래농업 유망분야에 대한 친숙도와 선호도를 바탕으로 수정된 IPA 분석을 실시하여 미래농업과 관련된 교육수요를 확인하였다. 결과 전문가 자문실시 결과 16개의 미래농업 유망분야가 도출되었으며, 신품종 개발과 같이 높은 수준의 지식과 기술을 요구하는 것을 제외하고, 고등학교 단계에서의 인력양성 필요성이 인정되었다. 도출된 16개의 미래농업 유망분야에 대한 서울시 교육주체의 교육수요를 분석한 결과, 농산물이력정보, 반려동물, 도시농업 등의 미래농업 유망분야에 대해 교육수요를 가지는 것으로 나타났다. 결론 이러한 연구결과는 중등단계에서부터 학생들이 미래농업 유망분야로 진출할 수 있도록 교양 수준의 교육과 진로교육, 나아가 전문적인 직업교육 등이 이루어질 필요가 있으며, 특히 미래농업 유망분야 인력양성을 위한 농업계열 중등교육기관이 존재하지 않는 지역인 서울시에서도 향후 미래농업 종사인력을 양성할 수 있는 교육과정 혹은 교육기관을 마련할 필요가 있음을 시사한다. Objectives The purpose of this study is to derive promising fields for future agriculture and to confirm whether the necessity of education is recognized at the secondary level in promising fields of future agriculture by experts and to confirm the demand for education in promising fields of future agriculture for students, parents, and teachers of middle schools located in Seoul. Methods To achieve the purpose first, a promising field for future agriculture was derived by conducting a literature review and expert advice. And then experts have confirmed the necessity of secondary education by using the importance of future agriculture, the sufficient of workforce, and the necessity of workforce development at the high school level. Results As a result, 16 promising fields of future agriculture were derived, and except for those requiring high-level knowledge and skills, such as the development of new varieties, the necessity workforce development at the high school level was recognized. Second, a revised IPA analysis was conducted using familiarity and preference for promising fields in future agriculture for educational participants to confirm the education demand related to future agriculture. As a result, it was found that there was a demand for education in promising fields of future agriculture such as agricultural product history information, companion animals, and urban agriculture. Conclusions This study's results contribute to the necessary to provide liberal education and career education, as well as vocational education, to help students choose a career path from secondary school to a promising agricultural field. In particular, the Seoul, which does not have agricultural secondary education institutions to foster promising fields of future agriculture, suggests that it is necessary to prepare a curriculum or educational institution to foster future agricultural workers in the future.

미국의 폐기물 매립분야 온실가스 배출량 산정 방법 활용 현황 분석

전채원 ( Chaewon Jeon ),김예린 ( Yelin Kim ),고가영 ( Gayoung Ko ),정상재 ( Sangjae Jeong ) 한국폐기물자원순환학회 2022 한국폐기물자원순환학회 심포지움 Vol.2022 No.1

미국의 폐기물 매립분야 온실가스 배출량 산정 방법 활용 현황 분석

전채원 ( Chaewon Jeon ),김예린 ( Yelin Kim ),고가영 ( Gayoung Ko ),정상재 ( Sangjae Jeong ) 한국폐기물자원순환학회 2022 한국폐기물자원순환학회 춘계학술발표논문집 Vol.2022 No.-

Success of Embryo Transfer Depends on Tight Junction Biogenesis and Assembly of Blastocyst

Inchul Choi,Jiyeon Jeong,Yelin Jeong,Jeeahn Lee 한국수정란이식학회 2017 한국수정란이식학회 학술대회 Vol.2017 No.05

Establishment of the Adherens junction (AJ) and Tight junction (TJ) are important steps in terms of morphological formation during preimplantation develoment. Particularly, TJ complex is crucial for cavitation in blastocyst. So far, many TJ protein/genes are revealed. However, the biological function and regulation of TJ were not elucidated during post implantation. We depleted several TJ and TJ associated genes using RNA interference, and examined preimplantation development with TJ. We tested functionality of paracellular sealing to determine integrity of TJ formation and examined TE differentiation indirectly using outgrowth assay in vitro. We observed defect of paracellular permeability in the TJ related genes knockdown(KD) blastocyst and abnormal outgrowth. Particularly, trophoblast cells were not stretched out in the KD groups. Finally, we did embryo transfer using the TJ genes KD and control blastocysts into surrogate mothers. We found lower of the implantation rates/ maintenance of pregnancy in the TJ KD groups (less than 40%) than in the controls (about 80%). In conclusion, TJ integrity is can be used as a selective marker for developmentally competent embryos and successful pregnancy.