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Lysobacter panacihumi sp. nov., isolated from ginseng cultivated soil
HUO YUE,강종표,Joon Hurh,Yaxi Han,안종찬,MATHIYALAGANRAMYA,Chunhong Piao,양덕춘 한국미생물학회 2018 The journal of microbiology Vol.56 No.10
A Gram-negative, non-motile, aerobic, catalase-, and oxidasepositive bacterial strain, designated DCY117T, was isolated from ginseng cultivated soil in Gochang-gun, Republic of Korea, and was characterized taxonomically using a multifaceted approach. 16S rRNA gene sequence analysis revealed that strain DCY117T showed highest similarity to Lysobacter ruishenii CTN-1T (95.3%). Phylogenetic analysis revealed that closely related relatives of strain DCY117T were L. aestuarii S2-CT (95.1%), L. daejeonensis GH1-9T (95.0%), and L. caeni BUT-8T (94.9%). Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE) were the major polar lipids of strain DCY117T. The major isoprenoid quinone was Q-8. The major cellular fatty acids of strain DCY117T were iso-C15:0, iso-C16:0, and summed feature 9 (comprising iso-C17:1 ω9c and/or 10-methyl-C16:0). Genomic DNA G + C content was 61.8 mol%. On the basis of our findings, strain DCY117T is a novel species in the genus Lysobacter. We propose the name Lysobacter panacihumi sp. nov., and the type strain is DCY117T (= KCTC 62019T = JCM 32168T).
음나무 뿌리를 이용한 산화 아연 나노파티클 생합성 및 메틸렌 블루의 분해를 위한 광촉매 활성
Indra Batjikh,Esrat Jahan Rupa,Veronika Soshnikova,Gokulanatha Anandapadmanaban,김연주,Yaxi Han,Hashmoonah Ali,양덕춘 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : For the green approach of nanoparticles synthesizing, plant based technology has been considered as cost-effective and eco-friendly mass production. The oriental medicinal crop, Kalopanax septemlobus (Thunb.) Koidz. (Korean name: 음나무), the deciduous tree and a family of Araliaceae. Endemic tree of Asian countries, K. septemlobus being used for the treatment of various diseases. Phytochemicals of K. septemlobus such as polyphenols has highly probability of reducing agent for biosynthesizing nanoparticles. Methods and Results : In this study, we applied K. septemlobus ZnO nanoparticles (Ks-ZnO NPs) with procedures including green approach one-pot synthesis. For the characterization of nanoparticles, UV–Vis, FTIR, XRD, SEM and TEM were used. The formation of ZnO nanoparticles, the aurface plasmon resonance were observed at 372 ㎚ in UV-Vis spectroscopy. The presence of functional groups which as a capping agent and formation of ZnO nanoparticles were confirmed in FTIR result. The crystallization and morphology showed by XRD, TEM and SEM respectively. The photocatalytic activity of ZnO nanoparticles, was determined using Methylene blue (MB) dye degradation under UV irradiation (365 ㎚) which resulted rate constant is (−k) 0.1215 with 97.5% of degradation in 30 min. Conclusion : The result shows that phytochemicals in K. septemlobus extract have a potential as a reducing agent to form ZnO nanoparticles. The ZnO NPs are capable to degrade MB with in brief time.
Li, Jin Feng,Rupa, Esrat Jahan,Hurh, Joon,Huo, Yue,Chen, Ling,Han, Yaxi,Ahn, Jong chan,Park, Jin Kyu,Lee, Hyun A.,Mathiyalagan, Ramya,Yang, Deok-Chun Elsevier 2019 Optik Vol.183 No.-
<P><B>Abstract</B></P> <P>Zinc Oxide nanoparticles were synthesized via co precipitation methods from <I>Cordyceps militaris</I> fungus. In this method zinc nitrate hex- hydrate were used as a precursor. The Synthesized nanoparticles were characterized through different analytical method for identification of morphology, size, crystallinity and also purity. The nanoparticles gave highest peak at 350 in Uv–vis spectroscopy which confirmed the formation of <I>Cordyceps</I> zinc oxide nanoparticles. The Field emission transmission electron microscopy (FE-TEM) analyzed the morphology, purity and elemental distribution of the nanoparticles. The X-ray powder diffraction (XRD) study explored the size is about 10.15 nm. Finally, the functional group of plant and nanoparticles were identified using FT-IR analyzer. The <I>Cordyceps militaris</I> zinc oxide nanoparticles have ability to degrade hazardous dye Methylene blue about 97% in the presence of Uv-radiation. So this study emphasizes that the <I>Cordyceps militaris</I> zinc oxide nanoparticles can be the best photo catalyst for the safety of our environment from dangerous water pollution.</P>
Shigui Huang,Shunchuan Wu,Huajin Zhang,Jiaxin Wang,Xiong Song,Yaxi Shen,Longqiang Han 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.12
In order to make up for the shortcomings of traditional discontinuity occurrence clustering algorithms, such as narrow application scope, difficulty in effectively identifying noise points, and susceptibility to initial conditions and hyperparameters. In this work, the dominant partitioning of rock masses discontinuities based on information entropy selective heterogeneous ensemble (DRIE) model is proposed. Firstly, the base cluster set is generated by four base-clustering algorithms. Secondly, the comprehensive quality evaluation index is obtained by evaluating the quality and difference of the base cluster, and the information entropy weighting matrix is integrated. Finally, the number of integrated base clusters and the optimal number of groups are determined, and the noise points are eliminated. In addition, this work lists four base-clustering algorithm models, establishes nine comparison models, and applies all models to the dominant partitioning of an adit discontinuity of Songta Hydropower Station in Tibet, China. The results show that the DRIE model and the above 13 models are evaluated by combining the silhouette index and the ability to identify and eliminate noise. Compared with other models, the DRIE model has wide applicability and objective grouping results. It provides a new idea for the study of the dominant occurrence grouping of discontinuity.
Kim, Chun Gon,Castro-Aceituno, Veró,nica,Abbai, Ragavendran,Lee, Hyun A.,Simu, Shakina Yesmin,Han, Yaxi,Hurh, Joon,Kim, Yeon-Ju,Yang, Deok Chun Elsevier 2018 BIOMEDICINE AND PHARMACOTHERAPY Vol.99 No.-
<P><B>Abstract</B></P> <P>Siberian ginseng (<I>Eleutherococcus senticosus</I>) was used for the synthesis of an ecofriendly silver nanoparticle (Sg-AgNP), which has exhibited antibacterial, antioxidant effect and lower cytotoxicity to normal cells in comparison to human cancer cells. Although, the potential anticancer activity of Sg-AgNP has not been determined. In this study, two cancer cell lines were used to evaluate the cytotoxicity and apoptotic effect of Sg-AgNP along with the determination of the role of the Caspase-3 / p38 MAPK pathways. Results shown that Sg-AgNP reduced the cell viability of colon cancer cells HT29 and lung cancer cells A549. The cytotoxic effect was higher than the effect exhibited by a commercial silver nanoparticle and Cisplatin. Reactive oxygen species were observed to be superior in both cell lines in the presence of Sg-AgNPs than c-AgNPs and Cisplatin. It was observed an activation of <I>MAPK14</I> gene and phosphorylation of p38 MAPK protein in both cell lines induced by Sg-AgNPs treatment. Furthermore, induction of morphological changes in the nucleus was done by Sg-AgNPs at 10 μg/mL in both cell lines. On the other hands, the activation of <I>CASP3</I> gene and Caspase-3 protein was observed in HT29 cells but only at protein level in A549 cells. These results, suggest that Sg-AgNPs anticancer potential activity might be linked to the induction of apoptosis though the generation of ROS by activation of the Caspase-3/p38 MAPK pathway.</P>