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Yangha Kim,Eun-Ha Choi,Miae Doo,Joo-Yeon Kim,Chul-Jin Kim,Chong-Tai Kim,In-Hwan Kim 한국영양학회 2010 Nutrition Research and Practice Vol.4 No.4
Catecholamines are among the first molecules that displayed a kind of response to prolonged or repeated stress. It is well established that long-term stress leads to the induction of catecholamine biosynthetic enzymes such as tyrosine hydroxylase (TH) and dopamine β-hydroxylase (DBH) in adrenal medulla. The aim of the present study was to evaluate the effects of ginseng on TH and DBH mRNA expression. Repeated (2 h daily, 14 days) immobilization stress resulted in a significant increase of TH and DBH mRNA levels in rat adrenal medulla. However, ginseng treatment reversed the stress-induced increase of TH and DBH mRNA expression in the immobilization-stressed rats. Nicotine as a ligand of the nicotinic acetylcholine receptor (nAChR) in adrenal medulla stimulates catecholamine secretion and activates TH and DBH gene expression. Nicotine treatment increased mRNA levels of TH and DBH by 3.3- and 3.1-fold in PC12 cells. The ginseng total saponin exhibited a significant reversal in the nicotine-induced increase of TH and DBH mRNA expression, decreasing the mRNA levels of TH and DBH by 57.2% and 48.9%, respectively in PC12 cells. In conclusion, immobilization stress induced catecholamine biosynthetic enzymes gene expression, while ginseng appeared to restore homeostasis via suppression of TH and DBH gene expression. In part, the regulatory activity in the TH and DBH gene expression of ginseng may account for the anti-stress action produced by ginseng.
Effect of Thyroid Hormone on Lipogenesis in Rat White and Brown Adipocytes Culture System
Yangha Kim Moon 한국식품영양과학회 1998 Preventive Nutrition and Food Science Vol.3 No.4
Thyroid hormone (T₃) stimulates hepatic lipogenesis by increasing expression of genes, including acetyl-CoA carboxylase and fatty acid synthase. S14 protein, which is thought to be involved in lipid metabolism, appears to respond in parallel. Effects of T₃ on lipogenesis in white and brown adipose tissue are less clear, and may be complicated by indirect effects of the hormone. We developed an adipocytes system where the indirect effects of thyroid hormone are abolished and direct effects of T₃ on lipogenesis could be tested. Fat accumulation was measured by Oil-Red O staining. Insulin clearly enhanced fat accumulation by 2-fold. Isobutylmethylxanthine (IBMX) appeared to inhibit insulin-stimulated fat accumulation. Dexamethasone increased insulin-stimulated fat accumulation about 1.3-fold. Confluent adipocytes were cultured in serum-free medium or medium containing 10% fetal calf serum or 10% fetal calf serum stripped of thyroid hormone and lipogenesis, assessed by the incorporation of ³H₂O, was measured. Medium without serum or supplemented with T₃-depleted serum did not amplify the stimulatory effect or T₃ on lipogenesis compared to medium containing 10% fetal calf serum. Dexamethasone alone led to a decrease in lipogenesis of about 50% in white adipocytes and 25% in brown adipocytes. However, dexamethasone amplified the lipogenic response to T₃ by about 30% in white adipocytes and 60% in brown adipocytes. T₃ (1 μM) stimulated lipogenesis and acetyl-CoA carboxylase and fatty acid synthase mRNA levels up to 2-fold in both types of adipocytes. It seems that these adipocyte systems are a useful model to study the effects of hormones on lipogenic gene expression as well as lipogenesis.
Effect of Thyroid hormone on Lipogenesis in Rat White and Brown Adipocytes Culture System
Kim, Yangha -Moon The Korean Society of Food Science and Nutrition 1998 Preventive Nutrition and Food Science Vol.3 No.4
Thyroid hormone(T3) stimulates hepatic lipogenesis by increasing expression of genes, indluding acetyl-CoA carboxylase and fatty acid synthase. S14 protein, which is thougth to be involved in lipid metabolism , appears to respond in parallel . Effect of T3 on lipogenesis in white and brown adipose tissue are less clear, and may be complicated by indirect effects of the hormone. We developed an adipocytes system where the indirect effects of thyroid hormone are abolished and direct effects of T3 on lipogenesis could be tested. Fat accumulation was mesured by Oil-Red O staining. Insulin clearly enhanced fat accumulation by 2-fold . Isobutylemethylxanthie(IBMX) apeared to inhibit insulin -stimulated fat accumulation. Dexamethasone increased insulin-stimulatedfat accumulation about 1.3-fold. confluent adipocytes were cultured in serum-free medium or medium containing 10% fetal calf serum or 10% fetal calf serum stripped of thyroid hormone and lipogenesis, assessed by the incorporation of 3H2O , was measured. Medium without serum or supplemented with T3-depleted serum did not amplify the stimulatory effect of T3 on lipogenesis compared to medium containing 10% fetal calf seru. Dexamethasone alone led to a decrease inlopogenesis of about 50 % in white adipocytes and 25% in brown adipocytes. However, dexamethasone amplified the lipogenic respnse to T3 by about 30% in whit eadipocytes and 60% in brown adipocytes. T3(1$\mu$M) stimulated lipogenesis and acetyl-CoA carboxylase and fatty acid syntase mRNA levels up to 2 -fold in both types of adipocytes. It seems that these adipocytes systems are as useful model to study the effects of hormones on lipogenic gene expression as well as lipogenesis.
유양하(Yangha-Yu),이낙영(Nakyoung-Lee) 한국철도학회 2012 한국철도학회 학술발표대회논문집 Vol.2012 No.5
고속철도 KTX에 RAMS가 적용되어 도입된 이후 우리나라에서 제작되는 모든 철도차량은 차량의 종류마다 약간의 차이는 있지만 RAMS가 적용되고 있다. 새로 제작된 차량은 하자보증기간동안 신뢰성 검증을 하게 되며, 유지보수과정에서는 자체적으로 RCM을 적용하고 있다. RCM은 KTX 운행초기부터 적용을 시작하였으나, 정착하기 까지는 많은 시간이 소요되고 있고, 고속차량 운영 8년이 지난 지금까지도 신뢰성 확보를 위한 노력이 계속되고 있다. KTX 기술이전을 바탕으로 우리기술로 제작한 'KTX-산천'은 운행초기 잦은 고장으로 차량제작사인 로템과 운영사인 코레일이 많은 어려움을 겪었다. 본 논문에서는 철도차량의 특성 및 운영환경에 적합한 유지보수 체계인 RCRM(Reliability Centered Rolling stocks Maintenance)에 대해 제시하고자 한다. RAMS has been applied to all new manufactured rolling stock in Korea considering a little differences by rolling stock after adopting RAMS in KTX. All new manufactured rolling stocks has reliability verification, and it has applied its own RCM for maintenance procedure. RCM has applied from the start of KTX operation, but in takes many times to settle down and it has been trying efforts to secure reliability for 8 years since the operation of KTX train. KTX-Sancheon manufactured by our own technology has many problems by frequent failures in early operation period, and manufacturer Rotem and operator KORAIL has experienced great difficulties. This paper want to introduce RCRM (Reliability Centered Rolling stocks Maintenance) maintenance system suitable for the characteristics of rolling stock and operation circumstances.
[산ㆍ학ㆍ연 논단] 당뇨병연구를 위한 유전학적 접근 : 형질전환 마우스 모델
김양하(Yangha Kim) 한국식품영양과학회 2000 식품산업과 영양 Vol.4 No.3
Non-insulin-dependent diabetes mellitus (NIDDM) is characterized by insulin resistance and impaired insulin secretion. The transgenic technology, in which a specific gene can be introduced or deleted to study its function, has been established. A number of transgenic mice, altered the expression of genes potentially involved in insulin action or pancreatic β-cell function, have recently been developed to address questions concerning NIDDM. Transgenic mice model may help understanding the molecular basis of complex patho-physiologies of NIDDM. This review outlines the new insights obtained from the studies of transgenic mice that overexpress or show decreased expression of putative key genes involved in the regulation of insulin resistance and pancreatic β-cell function, therefore in the control of glucose homeostasis.