Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

Days to Germination and Effect of Growth Regulator on Rhizome Growth in Cymbidium goeringii Hybrid

Kang,Tae-Jin,Yang,Deok-Chun 한국자원식물학회 2003 Plant Resources Vol.6 No.2

Germination efficiency, such as days to germination and conditions for the hybrid seeds, was tested after hybrid seeds were sown on the medium. Seeds were germinated from 67 to 126 days after seeding in all cross combinations, and germination condition was different among hybrid combinations. There was big difference on days to germination based on the different media, that is, days to germination in the hyponex medium was shorter than that in the Knudson C medium. Mutants such as MMS and NaN₃ were used to cause mutation. Germination occurred with 0.1 % NaN₃ and MMS. However, even though germination occurred in other treatments (0.01 % and 0.2% of NaN₃ and MMS), brownish phenomenon was intense, or did not proceed and got worse after 4-5 months of seeding. In addition, it was performed to choose appropriate medium for the growth of Cymbidium rhizome whose media adaptability is more different than other orchids. Different concentration of NAA and Kinetin was used. As a result, C. goeringii hybrid showed difference for the concentration of NAA and Kinetin combination.

The Effect of Cultural Factors on Anther Culture in Spring Wheat (Triticum aestivum)

Kang,Tae-Jin,Yang,Deok-Chun 한국자원식물학회 2003 Plant Resources Vol.6 No.2

Effects of shaking, medium consistency and anther density on polyhaploid production in two wheat cultivars, Pavon and Chris, were studied using a modified 85D12 medium. Pavon produced more calli in shaking and more albino plants tban Chris. However, Chris produced threefold more green plants than Pavon in non-shaking treatment. More calli and green plants were derived from non-shaking treatment than those from shaking treatment. Anthers were cultured on both liquid and semi-solid 85D12 media, using two anther densities, 48 and 96 anthers per plate. Although Pavon generally produced more calli and albino plants than Chris, Chris produced more green plants than Pavon. More green plants were derived from semi-solid medium than those from liquid medium. A factor that may affect plant regeneration from anthers is the length of time on initiation medium. Most of the calli for both genotypes were transferred during the first two time periods. Fertility, as measured by seed set, was determined for all surviving regenerated plants. About 24% of Chris and Pavon anther-derived green plants in the experiment of medium consistency and anther density produced seed.

초·중학교 육상선수의 참여형태에 따른 참여동기와 만족도

강양구,오수일 江原大學校附設體育科學硏究所 2004 江原大學校附設體育科學硏究所論文集 Vol.- No.27

The purpose of this study was to provide hilpful basic data for P.E. teachers making efforts to find and cultivate players and personnel concerned contributing to operating a track&field contest and developing programs by examining. Participation motive and satisfaction by track players' Participation type in elementary and middel school. A questionnaire survey was conducted with subjects of 444 persons in total 17 groups. 5 point Likert scale was used for the survey. Collected materials was calculated with standard deviation and average by sex, school grade and Participation type. Also t-test was applied to analyse the result. The results of the study are as follow: First of all, women were higer than men in Participation motive by whole sex based on questions related to health and sociality. Second, respondents of middle school showed higher Participation motive than those of elementary school by school grade based on questions related to relationships and performance ability. Third, long-term continuous Participants were higher than short-term Participants in Participation motive by Participation type based on questions related to health, achievement feelings and positive consideration, but that was contrary in questions related to performance ability. Fourth, men were higher than women in the degree of satisfaction based on questions concerned with interest, pride, health, professional intellect, facilities and place and teaching skill by whole sex. Fifth, middle school was higher than elementary school in the degree of satisfaction by school grade based on questions concerned with health, facilities and place. Lastly, long-term continuous Participants were higher than short-term Participants in the degree of satisfaction by Participation type based on questions concerned with health, interest, pride and specialized knowledge.

압궤손상을 입은 혈관에서 Ginkgo Biloba 추출물(EGb 761)이 혈관문합술후 개통율에 미치는 영향

강소라,이동진,차진한,김양우 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

Replantation after crush amputation has a relatively low success rate. Thrombus formation due to vessel and tissue trauma is considered as the principal cause of failure. In the laboratory and clinically, we have been tried to improve the post-anastomosis patency rate in crushed microvasculature. To accomplish this, we have usually used several anticoagulant drugs. Extracts from the leaves of Ginkgo biloba (EGb) have been used therapeutically for centuries. EGb exerts a number of pharmacologic actions. Eighty rats were control group and another 80 rats were treated with EGb 761. The femoral arteries underwent crush injury with an energy of 0.4J, and the vessles in each group were divided and anastomosed by a standard microsurgical technique. Each group was comprised as follows: ① control group(A1,A2): group A1(n=40); intraluminal saline irrigation, group A2(n=40); intraluminal saline irrigation+heparin 40 u/ml(IV) ② EGb 761 treated group(B1, B2): groupB1(n=40); intraluminal saline irrigation, group B2(n=40); intraluminal saline irrigation + heparin 40 u/ml(IV). At postoperative 14 days, the patency rates were; group A1 20%, group A2 77.5%, group B1 47.5%, group B2 92.5%. These results were interpreted as follows: the patency rate was significantly increased in the EGb 761-only treated group(p<0.01), the heparin-only treated group(p<0.01), and the EGb 761 and heparin-combined at crushed microvessel surgery. However the patency rate of the EGb-only treated group was significantly lower than that of the heparin-only treated group(p<0.01). And in the EGb 761 and heparin-combined treated group compared to the heparin-only treated group, there was some patency rate increase in the combined treated group, but there was no significant difference between them(p=0.060).

經濟人口學的ㆍ社會的 要因에 基礎한 特定地域人口 豫測模型

姜良錫 弘益大學校 1989 弘大論叢 Vol.21 No.2

. In setting city and regional development planning, population indicator is not only a basis for estimating the physical scale of the work, but also a helpful measure to envisage the economic and social changes induced by the development. Population growth in a region is distinguished as natural growth and social growth, and the latter is the one which varies conspicuously across time and place in a country which develops rapidly like Korea. Despite the foregoing fact, in estimating planned population in an actual planning work, the method of past growth trend is still made use of, because of the defficulties concerning the complicated factors surrounding the social growth. Whereas the method by past growth trend requires a small amount of data and a simple formula, a considerably big error can be made and, as a result, brings many obstacles to the planning and the carrying-out. This study proposes a future population estimation model base on economic demography. The model takes system dynamics as the basic tool and contains regional economy, mining and manufacturing industries, the tertiary industries, income and consumption, and population as the sub-systems. The framework of model is interpreted by using causal-diagram and equations.

정신분열병 환자에서 Olanzapine이 인지기능에 미치는 영향

강병조,김양태,김문두,이승재,박영우 대한생물치료정신의학회 2001 생물치료정신의학 Vol.7 No.1

Objects : The purpose of this study was to test the effects of olanzapine on cognitive functions in schizophrenic patients. Methods : The subjects were 16 haloperidol-medicated schizophrenic patients and 15 risperidone-medicated schizophrenic patients, who participated in an eight-week crossover study from haloperidol and risperidone to olanzapine. Results : The patients in the group of crossover from haloperidol to olanzapine showed significant improvement in Parkinson scale of ESRS and other cognitive functions except for verbal fluency. Negative scale of PANSS and cognitive functions such as verbal memory, verbal fluency, visual memory and executive function were significantly improved in patients In the group of crossover from risperidone to olanzapine. Conclusions : These data suggest that olanzapine has some superior cognitive benefits relative to haloperidol and risperidone, but a larger sample replication study is necessary.

방송산업과 저작권

강태영,최양수,김영석 연세대학교 언론연구소 1992 언론 사회 문화 Vol.- No.2

만성 부비동염에서 섬모운동과 전산화 단층촬영에서의 상악동 점막비후의 상관관계

민양기,전상준,이재서,이철회,이강수,윤자복,이원진,박광석 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

우루과이 벼농사와 쌀 수출산업 분석 : 1. 우루과이 벼농사 특징 Ⅰ. Rice Farming in Uruguay

강양순,신명철 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.1

우루과이 정부로부터 우루과이 국립농업연구소(INIA : Instituto Nacional de Investigacion Agropecuario Uruguay) 연구능력 강화를 위한 협력사업 요청을 받고 2003년 9월 20일부터 31일까지 우리 정부에 의해서 협력타당성 분석을 위한 우루과이 현지 조사와 자료분석이 이루어졌다. 우루과이 벼농사의 특징과 벼 재배환경 및 표준재배기술을 요약하면 다음과 같다. 1. 우루과이 벼농사의 특징은 우리와 반대계절에 재배되고 논이 따로 없이 목장에서 목초와 벼를 윤작체계로 하는 대규모 수출상업농이다. 2. 벼 재배환경은 기온이 비교적 낮고 토양 중인산과 규산함량이 극히 낮아서 냉해발생 빈도가 잦은 편이나 광활한 대평원과 풍부한 수자원으로 벼 생산에 아주 유리하여 제 3의 수출상품으로 발전시켰다. 3. 벼는 건답상태로 직파한 후에 담수재배하며 벼-목초-가축의 윤작체계로 지력유지와 잡초방제를 기하고 제초제와 비료사용을 최소화하여 환경 친화적으로 재배하며 특히 토양, 관개수, 쌀의 농약잔류분석을 체계적으로 실시하여 환경농업을 철저히 실천하는 표준재배를 하고 있다. Korean government received the requirement of cooperative project for strengthening of rice research ability to INIA (Instituto Nacional de Investigacion Agropecuario, Uruguay). A field investigation was undertaken in Uruguay and analysis of research papers were carried out from September 20-30, 2003 to determine the cooperative appropriateness by Korean government. The characters of rice farming in Uruguay that has the rotation cropping system based on rice-forage crops such as rye grass, lotus and red clover in pasture is a very important exporting industry. It is developing as the 3rd industry goods in Uruguay by having the favorable cultivation environments with vast plain and a plenty of water for rice production, although low temperature and low contents of phosphate and silicic acid in soil related to cool injury of rice occurred during rice growing period. The rice farming is managed by friendly-environment theregy reducing the cost, maintaining of soil fertility, control of weeds and minimizing use of fertilizer by the rice-forage crop-cattle complex farming system. Rice is seeded in dry condition and then irrigated by 3㎝ depth of water. Especially, the rice cropping is practicing the sustainable agriculture system by keeping off the standard culture that has applied 70㎏ per ha of nitrogen and no potassium, and by the analytical operating of the residual agricultural chemicals to soil, irrigation water and rice grains.