Assessment of nephrotoxicity of herbal medicine containing aristolochic acid in mice

Yi Quan,Long Jin,Kang Luo,Jian Jin,Sun Woo Lim,신유진,Eun Jeong Ko,Byung Ha Chung,Chul Woo Yang 대한내과학회 2020 The Korean Journal of Internal Medicine Vol.35 No.2

Background/Aims: It is undetermined if herbal medicines (HM) containing aristolochic acid (AA)-containing have similar nephrotoxicity to AA itself. Methods: We administered HM containing a high concentration of AA for 5 days (short-term study) or a low concentration of AA for 30 days (long-term study) to C57BL/6 mice; for comparison, same dose of AA compound was used as controls. Results: The nephrotoxicity in the HM- and AA-treated mice was compared in terms of renal function, histopathology, oxidative stress, apoptotic cell death, and mitochondrial damage. Short-term HM treatment resulted in acute kidney injury (marked renal dysfunction, acute tubular necrosis, and neutrophil gelatinase-associated lipocalin [NGAL] expression) in which the severity of renal dysfunction and histopathology was comparable with that induced by the administration of AA alone. Long-term HM treatment resulted in features of chronic kidney disease (CKD, mild renal dysfunction and tubular atrophy and dilatation). No significant differences in these parameters were observed between the HM- and AA-treated mice. HM-induced oxidative stress (8-hydroxy-2’-deoxyguanosine and manganese- dependent superoxide dismutase expression) and apoptotic cell death (terminal deoxynucleotidyl transferase dUTP nick end labelling [TUNEL]-positive cells and active caspase-3 expression) were similar in HM- and AA-treated mice in the short-term and long-term studies. Mitochondrial injury, evaluated by electron microscopy, was also similar in HM- and AA-treated mice in the short-term and long-term studies. Conclusions: The nephrotoxic potential of HM containing AA was similar to that of AA itself.

Ginseng extract reduces tacrolimus-induced oxidative stress by modulating autophagy in pancreatic beta cells

Lim, Sun Woo,Jin, Long,Luo, Kang,Jin, Jian,Yang, Chul Woo Nature Publishing Group 2017 Laboratory investigation Vol.97 No.11

<P>We previously reported that long-term treatment with a calcineurin inhibitor impairs autophagy process in pancreatic beta cells. This study investigated the effect of Korean red ginseng extract (KRGE) on autophagy modulated by oxidative stress. In mice with tacrolimus (Tac)-induced diabetes mellitus, KRGE alleviated islet dysfunction and decreased oxidative stress and autophagic vacuoles. In vitro, KRGE decreased autophagosome formation and attenuated lysosomal degradation, accompanied by improved beta cell viability and insulin secretion. Addition of 3-methyladenine (3-MA), an inhibitor of autophagosomes, to KRGE further improved cell viability and insulin secretion, and bafilomycin A (BA), an inhibitor of lysosomal function, reduced the effects of KRGE. At the subcellular level, Tac caused mitochondrial dysfunction (impaired mitochondrial oxygen consumption, ATP production, and increased reactive oxygen species production). But KRGE improved these parameters. The effect of KRGE on mitochondrial function enhanced by 3-MA but decreased by BA, suggesting a causal relationship between KRGE effect and autophagy modulation in Tac-induced mitochondrial dysfunction. These findings indicate that KRGE modulates autophagy favorably by reducing Tac-induced oxidative stress, and this effect is closely associated with improvement of mitochondrial function.</P>

RPK118, a PX Domain-containing Protein, Interacts with Peroxiredoxin-3 through Pseudo-Kinase Domains

Long Yu,Lingling Liu,Chenyi Yang,Jian Yuan,Xiujuan Chen,Jianing Xu,Youheng Wei,Jingchun Yang,Gang Lin 한국분자세포생물학회 2005 Molecules and cells Vol.19 No.1

RPK118 is a sphingosine kinase-1-binding protein that has been implicated in sphingosine 1 phosphate-mediated signaling. It contains a PX (phox homology) domain and two pseudo-kinase domains, and co-localizes with sphingosine kinase-1 on early endosomes. In this study we identified a novel RPK118-binding protein, PRDX3 (peroxiredoxin-3), by yeast two-hybrid screening. The interaction between these proteins was confirmed by pull-down assays and co-immunoprecipitation experi-ments. Deletion studies showed that RPK118 inter-acted with PRDX3 through its pseudokinase domains, and with early endosomes through its PX domain. Dou-ble immunofluorescence experiments demonstrated that PRDX3 co-localized with RPK118 on early endosomes in COS7 cells. PRDX3 is a member of the antioxidant family of proteins synthesized in the cytoplasm and functioning in mitochondria. Our findings indicate that RPK118 is a PRDX3-binding protein that may be involved in transporting PRDX3 from the cytoplasm to its mitochondrial site of function or to other mem-brane structures via endosome trafficking

일반논문 : 중국의 도시화 과정과 도시문화연구

양지엔롱 ( Jian Long Yang ) 서울시립대학교 도시인문학연구소 2013 도시인문학연구 Vol.5 No.2

모두가 목도하고 있듯, 중국의 도시화 과정은 빠르게 진행되고 있다. 이에 도시문화연구는 중국 도시화 과정에 있어 중요한 임무가 되었다. 해외의 도시문화연구 성과를 번역, 소개하는 일은 국내 도시문화연구에 있어 매우 중요한 과제이며, 도시문화연구의 시야를 넓히고 이론을 확립하는 데에 새로운 의의를 갖는다. 대학 기관과 사회과학원으로 구성된 연구 기관은 도시문화연구와 발전에 중요한 역할을 하고 있다. 그러나 최근 들어 도시문화연구 과정에서 여전히 부족함이 드러나고 있다. 우선, 이론에 있어 체계성이 떨어지고 이론 체계를 수립하고자 하는 시야가 부족하다. 또한, 거시적 연구에 주목하면서 구체적인 문제를 연구하는 세밀함이 부족하다. 마지막으로 주로 정치학, 경제학 등의 방법에 주목할 뿐, 필드워크를 통한 깊이 있는 조사가 부족하다. The rapid pace of urbanization in China is obviously, research on urban culture has become an important task in the urbanization process in China. Translation of foreign urban culture research is important task of the cultural research, cultural studies, as the threshold for the expansion of the city, the construction of urban culture theory, a pioneering significance. Institutions of higher learning and research institutions set up by the Academy of Social Sciences, has played a very important role in the research and development of urban culture. In the study of urban culture in recent years, we still see some shortcomings and deficiencies. First of all, in theory, the lack of systematic, lack of achievements theory system as the threshold. Secondly, the overall focus on the macro, the lack of in-depth research on specific detailed. Third, the study is often by political science, economics, the lack of specific in-depth fieldwork.

miR-9 Modulates Osteosarcoma Cell Growth by Targeting the GCIP Tumor Suppressor

Zhu, Shao-Wen,Li, Jian-Peng,Ma, Xin-Long,Ma, Jian-Xiong,Yang, Yang,Chen, Yang,Liu, Wei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

Osteosarcoma is the most common primary bone tumor in humans, especially in childhood. However, the genetic etiology for its pathogenesis remains elusive. It is known that microRNAs (miRNAs) are involved in the development of tumor progression. Here we show that microRNA-9 (miR-9) is a potential oncogene upregulated in osteosarcoma cells. Knockdown of miR-9 in osteosarcoma resulted in suppressed colony formation and cell proliferation. Further study identified GCIP, a Grap2 and cyclin D interacting protein, as a direct target of miR-9. In addition, GCIP overexpression activated retinoblastoma 1 (Rb) and suppressed E2F transcriptional target expression in osteosarcoma cells. Moreover, GCIP depletion reversed miR-9 knockdown induced colony formation and cell proliferation suppression. In sum, these results highlight the importance of miR-9 as an oncogene in regulating the proliferation of osteosarcoma by directly targeting GCIP and may provide new insights into the pathogenesis of osteosarcoma.

Sliding Mode Control of Three-Phase Four-Leg Inverters via State Feedback

Long-Yue Yang,Jian-Hua Liu,Chong-Lin Wang,Gui-Fu Du 전력전자학회 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.5

To optimize controller design and improve static and dynamic performances of three-phase four-leg inverter systems, a compound control method that combines state feedback and quasi-sliding mode variable structure control is proposed. The linear coordinate change matrix and the state variable feedback equations are derived based on the mathematical model of three-phase four-leg inverters. Based on system relative degrees, sliding surfaces and quasi-sliding mode controllers are designed for converted linear systems. This control method exhibits the advantages of both state feedback and sliding mode control. The proposed controllers provide flexible dynamic control response and excellent stable control performance with chattering suppression. The feasibility of the proposed strategy is verified by conducting simulations and experiments.

Characterization of a Thermophilic Hemoglobin-degrading Protease from Streptomyces rutgersensis SCSIO 11720 and Its Application in Antibacterial Peptides Production

Jian Yang,Jie Li,Yunfeng Hu,Lizhen Li,Lijuan Long,Fazuo Wang,Si Zhang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1

High value utilization of byproduct of slaughterhousesis regarded as beneficial to both economy andenvironment. A hemoglobin degrading protease PA720was purified from fermentation broth of a Porites luteaassociated strain Streptomyces rutgersensis SCSIO 11720(SCSIO 11720) and exhibited maximal activity at 70°C,pH 10.5. The isolated strain was identified by genotypicmethod and 16 s RNA gene sequence was deposited inGenBank under accession No. KC414842. The enzymeexhibited excellent thermostability since it was stable at 70°Cwith t1/2 value of 1,386.3 min. Kinetic and thermodynamicstudy showed the activation energy (Ea), ΔG* (free energy),ΔH* (enthalpy) and ΔS* (entropy) for protease deactivationwere 62.71 kJ/mol, 74.26 kJ/mol, 59.86 kJ/mol, and-41.97 J/mol/K, respectively. Highest specific activity ofprotease PA720 was observed when using casein (9,953 U/mg)and hemoglobin (9,854 U/mg) as substrates. Hemoglobinhydrolysate prepared by protease PA720 showed significantantibacterial activity towards Escherichia coli, Staphylococcusaureus and Bacillus subtilis, indicating this protease couldbe used as an instrumental enzyme for production ofhemoglobin-based antibacterial peptides. Based on partialamino acid sequences of the enzyme PA720, the full geneencoding this protease was obtained by degenerate primerPCR and has been deposited in GenBank under accessionNo. KC414842. The deduced amino acid sequence exhibitshomology with other microbial thermophilic proteases insubtilisin family.

Distributed Functional Observer-based Event-triggered Containment Control of Multi-agent Systems

Long Jian,Jiangping Hu,Jun Wang,Kaibo Shi,Zhinan Peng,Yaoru Yang,Jiuke Huang 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.5

This paper studies the containment control problem of linear multi-agent systems (MASs) with a directed graph, where the states of the followers will asymptotically converge to the convex hull formed by those of the leaders. By introducing the functional observer, a novel distributed event-triggered control strategy is presented to schedule communications between agents, which depends on the relative input and output measurements with its neighbors. Furthermore, the minimum inter-event time is guaranteed to be strictly positive. An example is presented to illustrate the feasibility and efficiency of the theoretic results.

A Novel Phase Locked Loop for Grid-Connected Converters under Non-Ideal Grid Conditions

Yang, Long-Yue,Wang, Chong-Lin,Liu, Jian-Hua,Jia, Chen-Xi The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.1

Grid synchronization is one of the key techniques for the grid-connected power converters used in distributed power generation systems. In order to achieve fast and accurate grid synchronization, a new phase locked loop (PLL) is proposed on the basis of the complex filter matrixes (CFM) orthogonal signal generator (OSG) crossing-decoupling method. By combining first-order complex filters with relation matrixes of positive and negative sequence voltage components, the OSG is designed to extract specific frequency orthogonal signals. Then, the OSG mathematical model is built in the frequency-domain and time-domain to analyze the spectral characteristics. Moreover, a crossing-decoupling method is suggested to decouple the fundamental voltage. From the eigenvalue analysis point of view, the stability and dynamic performance of the new PLL method is evaluated. Meanwhile, the digital implementation method is also provided. Finally, the effectiveness of the proposed method is verified by experiments under unbalanced and distorted grid voltage conditions.

Sliding Mode Control of Three-Phase Four-Leg Inverters via State Feedback

Yang, Long-Yue,Liu, Jian-Hua,Wang, Chong-Lin,Du, Gui-Fu The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.5

To optimize controller design and improve static and dynamic performances of three-phase four-leg inverter systems, a compound control method that combines state feedback and quasi-sliding mode variable structure control is proposed. The linear coordinate change matrix and the state variable feedback equations are derived based on the mathematical model of three-phase four-leg inverters. Based on system relative degrees, sliding surfaces and quasi-sliding mode controllers are designed for converted linear systems. This control method exhibits the advantages of both state feedback and sliding mode control. The proposed controllers provide flexible dynamic control response and excellent stable control performance with chattering suppression. The feasibility of the proposed strategy is verified by conducting simulations and experiments.