Galectin-9 Acts as a Prognostic Factor with Antimetastatic Potential in Hepatocellular Carcinoma

Zhang, Zhao-Yang,Dong, Jia-Hong,Chen, Yong-Wei,Wang, Xian-Qiang,Li, Chong-Hui,Wang, Jian,Wang, Guo-Qiang,Li, Hai-Lin,Wang, Xue-Dong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Considerable research has been conducted concerning galectin-9 and carcinomas, but little information is available about any relation with the hepatocellular carcinoma. In this study, we employed a small interfering RNA (siRNA) targeting galectin-9 to down-regulate the expression in HepG2 cells. As a result, after galectin-9 expression was reduced, cell aggregation was suppressed, while other behaviour such as the proliferation, adhesion and invasion to ECM, cell-endothelial adhesion and transendothelial invasion of the cells were markedly enhanced. When tumors of 200 patients with hepatocellular carcinoma were tested for galectin-9 expression by immunohistochemistry, binding levels demonstrated intimate correlations with the histopathologic grade, lymph node metastasis, vascular invasion and intrahepatic metastasis (P<0.05). Moreover, survival analysis indicated that patients with galectin-9 expression had much longer survival time than those with negative lesions, and the Log-rank test indicated that this difference was statistical significant (P<0.0001). The Cox proportional hazards model suggested that negative galectin-9 expression in hepatocellular carcinoma represented a significant risk factor for patient survival. We propose that galectin-9 might be a new prognostic factor with antimetastatic potential in patients with hepatocellular carcinoma.

Cloning, Annotation and Expression Analysis of Mycoparasitism- Related Genes in Trichoderma harzianum 88

Lin Yao,Qian Yang,Jinzhu Song,Chong Tan,Changhong Guo,Li Wang,Lianhai Qu,Yun Wang 한국미생물학회 2013 The journal of microbiology Vol.51 No.2

Trichoderma harzianum 88, a filamentous soil fungus, is an effective biocontrol agent against several plant pathogens. High-throughput sequencing was used here to study the mycoparasitism mechanisms of T. harzianum 88. Plate confrontation tests of T. harzianum 88 against plant pathogens were conducted, and a cDNA library was constructed from T. harzianum 88 mycelia in the presence of plant pathogen cell walls. Randomly selected transcripts from the cDNA library were compared with eukaryotic plant and fungal genomes. Of the 1,386 transcripts sequenced, the most abundant Gene Ontology (GO) classification group was “physiological process”. Differential expression of 19 genes was confirmed by real-time RT-PCR at different mycoparasitism stages against plant pathogens. Gene expression analysis revealed the transcription of various genes involved in mycoparasitism of T. harzianum 88. Our study provides helpful insights into the mechanisms of T. harzianum 88-plant pathogen interactions.

Long-term Survivals, Toxicities and the Role of Chemotherapy in Early-Stage Nasopharyngeal Carcinoma Patients Treated with Intensity-modulated Radiation Therapy: A Retrospective Study with 15-year Follow-up

Lin Wang,Jingjing Miao,Huageng Huang,Boyu Chen,Xiao Xiao,Manyi Zhu,Yingshan Liang,Weiwei Xiao,Shaomin Huang,Yinglin Peng,Xiaowu Deng,Xing Lv,Weixiong Xia,Yanqun Xiang,Xiang Guo,Fei Han,Chong Zhao 대한암학회 2022 Cancer Research and Treatment Vol.54 No.1

Purpose This study was aimed to investigate long-term survivals and toxicities of early-stage nasopharyngeal carcinoma (NPC) in endemic area, evaluating the role of chemotherapy in stage II patients. Materials and Methods Totally 187 patients with newly diagnosed NPC and restaged American Joint Committee on Cancer/ International Union Against Cancer 8th T1-2N0-1M0 were retrospectively recruited. All received intensity-modulated radiotherapy (IMRT)±chemotherapy (CT) from 2001 to 2010. Results With 15.7-year median follow-up, 10-year locoregional recurrence-free survival, distant metastasis-free survival (DMFS), disease-specific survival (DSS), and overall survival (OS) were 93.3%, 93.5%, 92.9% and 88.2%, respectively. Multivariable analyses showed cervical lymph nodes positive and pre-treatment prognostic nutritional index ≥ 52.0 could independently predict DMFS (p=0.036 and p=0.011), DSS (p=0.014 and p=0.026), and OS (p=0.002 and p < 0.001); Charlson comorbidity index < 3 points could predict DSS (p=0.011); age > 45 years (p=0.002) and pre-treatment lactate dehydrogenase ≥ 240 U/L (p < 0.001) predicted OS. No grade 4 late toxicity happened; grade 3 late toxicities included subcutaneous fibrosis (4.3%), deafness or otitis (4.8%), skin dystrophy (2.1%), and xerostomia (1.1%). No differences on survivals were shown between IMRT+CT vs. IMRT alone in stage II patients, even in T2N1M0 (p > 0.05). Unsurprising, patients in IMRT+CT had more acute gastrointestinal reaction, myelosuppression, mucositis, late ear toxicity, and cranial nerve injury (all p < 0.05) than IMRT alone group. Conclusion Superior tumor control and satisfying long-term outcomes could be achieved with IMRT in early-stage NPC with mild late toxicities. As CT would bring more toxicities, it should be carefully performed to stage II patients.

Fabrication of Water-Soluble CuInS2 Quantum Dots by Hot-injection Method and Phase Transfer Strategy

Chong Deng,Bowen Fu,Yanlai Wang,Lin Yang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.10

Here we report an optimized hot-injection method and a phase transfer strategy for the synthesis of water-soluble CuInS2 QDs with desired properties. The structure and morphology studies demonstrate that the resulting QDs are CuInS2 tetragonal phase with well-defined facets. It is also found that the crystal size gradually increases with the increase of reaction temperature, while the surface of QDs with pre- and post-phase transfer is functionalized with hydrophobic and hydrophilic ligands, respectively. Spectroscopy measurements reveal the size-dependent optical properties of CuInS2 QDs, demonstrating the quantum confinement effect in this system.

Up-regulation of NHE8 by somatostatin ameliorates the diarrhea symptom in infectious colitis mice model

Xuelian Lei,Lin Cai,Xiao Li,Hua Xu,Chong Geng,Chunhui Wang 대한약리학회 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.3

Na+/H+ exchangers (NHEs) have been shown to be involved in regulating cell volume and maintaining fluid and electrolyte homeostasis. Pooled evidences have suggested that loss of Na+/H+ exchanger isoform 8 (NHE8) impairs intestinal mucosa. Whether NHE8 participates in the pathology of infectious colitis is still unknown. Our previous study demonstrated that somatostatin (SST) could stimulate the expression of intestinal NHE8 so as to facilitate Na+ absorption under normal condition. This study further explored whether NHE8 participates in the pathological processes of infectious colitis and the effects of SST on intestinal NHE8 expression in the setting of infectious colitis. Our data showed that NHE8 expression was reduced in Citrobacter rodentium (CR) infected mice. Up-regulation of NHE8 improved diarrhea symptom and mucosal damage induced by CR. In vitro, a similar observation was also seen in Enteropathogenic E. coli (EPEC) infected Caco-2 cells. Seglitide, a SST receptor (SSTR) 2 agonist, partly reversed the inhibiting action of EPEC on NHE8 expression, but SSTR5 agonist (L-817,818) had no effect on the expression of NHE8. Moreover, SST blocked the phosphorylation of p38 in EPEC-infected Caco-2 cells. Taken together, these results suggest that enhancement of intestinal NHE8 expression by SST could ameliorate the symptoms of mice with infectious colitis.

A Novel Phase Locked Loop for Grid-Connected Converters under Non-Ideal Grid Conditions

Long-Yue Yang,Chong-Lin Wang,Jian-Hua Liu,Chen-Xi Jia 전력전자학회 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.1

Grid synchronization is one of the key techniques for the grid-connected power converters used in distributed power generation systems. In order to achieve fast and accurate grid synchronization, a new phase locked loop (PLL) is proposed on the basis of the complex filter matrixes (CFM) orthogonal signal generator (OSG) crossing-decoupling method. By combining first-order complex filters with relation matrixes of positive and negative sequence voltage components, the OSG is designed to extract specific frequency orthogonal signals. Then, the OSG mathematical model is built in the frequency-domain and time-domain to analyze the spectral characteristics. Moreover, a crossing-decoupling method is suggested to decouple the fundamental voltage. From the eigenvalue analysis point of view, the stability and dynamic performance of the new PLL method is evaluated. Meanwhile, the digital implementation method is also provided. Finally, the effectiveness of the proposed method is verified by experiments under unbalanced and distorted grid voltage conditions.

Matrix Degrading enzymes of u-PA , PAI-1 and nm-23 Gene expression administering complex prescription of Huoxue Huayu

Gongshu Li,Hongsheng Lin,Leyu Wang,Zhenchun Bi,Chong Kwan Cho,Bingkui Piao 경희대학교 한의학연구소 1997 JOURNAL OF ORIENTAL MEDICINE Vol.2 No.1

Up-regulation of NHE8 by somatostatin ameliorates the diarrhea symptom in infectious colitis mice model

Lei, Xuelian,Cai, Lin,Li, Xiao,Xu, Hua,Geng, Chong,Wang, Chunhui The Korean Society of Pharmacology 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.3

$Na^+/H^+$ exchangers (NHEs) have been shown to be involved in regulating cell volume and maintaining fluid and electrolyte homeostasis. Pooled evidences have suggested that loss of $Na^+/H^+$ exchanger isoform 8 (NHE8) impairs intestinal mucosa. Whether NHE8 participates in the pathology of infectious colitis is still unknown. Our previous study demonstrated that somatostatin (SST) could stimulate the expression of intestinal NHE8 so as to facilitate $Na^+$ absorption under normal condition. This study further explored whether NHE8 participates in the pathological processes of infectious colitis and the effects of SST on intestinal NHE8 expression in the setting of infectious colitis. Our data showed that NHE8 expression was reduced in Citrobacter rodentium (CR) infected mice. Up-regulation of NHE8 improved diarrhea symptom and mucosal damage induced by CR. In vitro, a similar observation was also seen in Enteropathogenic E. coli (EPEC) infected Caco-2 cells. Seglitide, a SST receptor (SSTR) 2 agonist, partly reversed the inhibiting action of EPEC on NHE8 expression, but SSTR5 agonist (L-817,818) had no effect on the expression of NHE8. Moreover, SST blocked the phosphorylation of p38 in EPEC-infected Caco-2 cells. Taken together, these results suggest that enhancement of intestinal NHE8 expression by SST could ameliorate the symptoms of mice with infectious colitis.

A Novel Phase Locked Loop for Grid-Connected Converters under Non-Ideal Grid Conditions

Yang, Long-Yue,Wang, Chong-Lin,Liu, Jian-Hua,Jia, Chen-Xi The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.1

Grid synchronization is one of the key techniques for the grid-connected power converters used in distributed power generation systems. In order to achieve fast and accurate grid synchronization, a new phase locked loop (PLL) is proposed on the basis of the complex filter matrixes (CFM) orthogonal signal generator (OSG) crossing-decoupling method. By combining first-order complex filters with relation matrixes of positive and negative sequence voltage components, the OSG is designed to extract specific frequency orthogonal signals. Then, the OSG mathematical model is built in the frequency-domain and time-domain to analyze the spectral characteristics. Moreover, a crossing-decoupling method is suggested to decouple the fundamental voltage. From the eigenvalue analysis point of view, the stability and dynamic performance of the new PLL method is evaluated. Meanwhile, the digital implementation method is also provided. Finally, the effectiveness of the proposed method is verified by experiments under unbalanced and distorted grid voltage conditions.

Sliding Mode Control of Three-Phase Four-Leg Inverters via State Feedback

Long-Yue Yang,Jian-Hua Liu,Chong-Lin Wang,Gui-Fu Du 전력전자학회 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.5

To optimize controller design and improve static and dynamic performances of three-phase four-leg inverter systems, a compound control method that combines state feedback and quasi-sliding mode variable structure control is proposed. The linear coordinate change matrix and the state variable feedback equations are derived based on the mathematical model of three-phase four-leg inverters. Based on system relative degrees, sliding surfaces and quasi-sliding mode controllers are designed for converted linear systems. This control method exhibits the advantages of both state feedback and sliding mode control. The proposed controllers provide flexible dynamic control response and excellent stable control performance with chattering suppression. The feasibility of the proposed strategy is verified by conducting simulations and experiments.