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Yan‑Li Li,Xi‑Qian Xing,Yi Xiao,Yan‑Hong Liu,Yu‑Shan Zhou,Min Zhuang,Chao‑Qian Li 한국유전학회 2020 Genes & Genomics Vol.42 No.12
Background: The overexpression of TSLP and DNA methylation in asthma were both risk factors the relationship was not clear. Objective: This study aimed to investigate the relationship between methylation status of TSLP promoter and mRNA/protein expression in asthmatic airway epithelial cells. Methods: Human bronchial epithelial cells were cultured in vitro and divided into: Control group, treated with PBS, model group, sensitized with LPS (10 μg/mL) for 12 h (37 °C, 5% CO2). Other groups were cultured with the pCMV3 plasmid (M + NC/pCMV), pGPH1 plasmid (M + NC/pGPH), DNMT1/pCMV3 plasmid (M + DNMT1/pCMV), and DNMT1/pGPH1 plasmid (M + DNMT1/pGPH) for 48 h. The expression of DNA methyltransferase 1 and TSLP were measured using real-time PCR and western blotting. Results: Compared with the control group, TSLP mRNA (1.00 ± 0.00 vs. 2.82 ± 0.81 vs. 1, P < 0.001) and protein (1.07 ± 0.04 vs. 1.46 ± 0.11, P < 0.01) were significantly greater, and the methylation of promoter was lower (92.75 ± 1.26 vs. 58.57 ± 3.34, P < 0.05) in the model group. Compared with the model group, TSLP mRNA (2.82 ± 0.81 vs. 1.17 ± 0.10, P < 0.001) decreased, but TSLP promoter methylation increased (58.57 ± 3.34 vs. 92.58 ± 7.30, P < 0.05) in M + DNMT1/pCMV. TSLP mRNA and protein were higher (2.82 ± 0.81 vs. 5.32 ± 0.21, P < 0.001; 1.46 ± 0.11 vs. 1.94 ± 0.11, respectively, P < 0.01), TSLP promoter methylation was lower (58.57 ± 3.34 vs. 33.57 ± 4.29, P < 0.05) in M + DNMT1/pGPH. Conclusions: Overexpression of TSLP in asthmatic airway epithelial cells may be regulated by DNA demethylation.
LPL gene Pvu II polymorphism and hypertriglycer-idemia: a meta-analysis involving 1,640 subjects
( Yan-yan Li ),( Yan-hong Zhou ),( Ge Gong ),( Hong-yu Geng ),( Xin-xing Yang ),( Xiang-ming Wang ),( Chuan-wei Zhou ),( Jian Xu ),( Yun Qian ) 대한내과학회 2017 The Korean Journal of Internal Medicine Vol.32 No.6
Background/Aims: Although lipoprotein lipase (LPL) gene Pvu II polymorphism has been associated with an increased risk of hypertriglyceridemia (HT), there is no clear consensus within the scientific community. Methods: A meta-analysis of 1,640 subjects from six individual studies was conducted to better elucidate the potential relationship between the LPL gene Pvu II polymorphism and HT within the Chinese population. Pooled odds ratios (ORs) and their corresponding 95% confidence intervals (CIs) were evaluated by using fixed effect models. Results: Our analysis indicated a significant association between LPL gene Pvu II polymorphism and HT within the Chinese population under allelic (OR, 1.550; 95% CI, 1.320 to 1.830; p = 1.158 × 10<sup>-7</sup>), recessive (OR, 0.540; 95% CI, 0.390 to 0.750; p = 0.0002), dominant (OR, 1.889; 95% CI, 1.501 to 2.377; p = 5.960 × 10<sup>-8</sup>), homozygous (OR, 2.167; 95% CI, 1.531 to 3.067; p = 1.242 × 10<sup>-5</sup>), heterozygous (OR, 1.810; 95% CI, 1.419 to 2.309; p = 1.842 × 10<sup>-6</sup>), and additive genetic models (OR, 1.553; 95% CI, 1.320 to 1.828; p = 1.158 × 10<sup>-7</sup>). Conclusions: Because LPL gene Pvu II restriction fragment length polymorphism polymorphism was associated with an elevated risk of HT, the P+ allele carriers of the LPL gene might be predisposed to HT.
Deng, Qian-Qian,Huang, Xin-En,Ye, Li-Hong,Lu, Yan-Yan,Liang, Yong,Xiang, Jin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1
Purpose: This phase II study was undertaken to determine the efficacy and safety of Loubo$^{(R)}$ (Lobaplatin) in combination with pemetrexed in treating patients with metastatic breast cancer who failed to respond to anthracycline or taxanes. Patients and Methods: Metastatic breast cancer cases who had previously received an anthracycline and a taxane in either adjuvant or metastatic settings, were enrolled. All patients were recruited from Jiangsu Cancer Hospital and Research Institute, and were treated with Loubo$^{(R)}$ (Lobaplatin) 35 $mg/m^2$ (intravenous; on day 1) and pemetrexed 500 $mg/m^2$ (intravenous; on day 1) every 21 days. Efficacy and side effects were evaluated after at least two cycles of chemotherapy. Results: All eligible 19 patients completed at least 2 cycles of chemotherapy with pemetrexed and lobaplatin, and were evaluable. Overall, 3 (15.8%) patients achieved partial response, 11 (57.9%) stable disease, 5 (26.3%) progression of disease, with no complete remission. Response rate was 15.8%, disease control rate was 42.1%. The median survival time was 10.3 months. Neutrophil suppression occurred in 36.8% of patients who had grade 2 toxicity, and 26.3% had grade 3, 26.4% had grade 4. Thrombocytopenia was encountered as follows: 21.1% grade 2, 15.8% grade 3 and 5.5% grade 4. Incidences of anemia were 10.5% in grade 2, 5.3% grade 3 and 0% grade 4. Only 5.3% of patients required packed red blood cell transfusion. Grade 3 digestive tract toxicity occurred in 5.5% of patients. Other toxicities included elevated transaminase,oral mucositis and skin rashes. Conclusions: The regimen of lobaplatin and pemetrexed is modestly active in metastatic breast cancer patients who failed anthracycline or taxanes, and the toxicity profile suggesting that the doses of chemotherapy should be further modified.
Qian Guo,Young-Suk Grace Kim,Yan Liu,Yan Peng,WENKAI SUN,Yijie Wang,Li Yang 서울대학교 교육연구소 2021 Asia Pacific Education Review Vol.22 No.1
Thanks to urbanization, some developing countries have a large migrant population. Academic performance of migrant children is usually far from satisfactory, much to the concern of educators and legislators. Meanwhile, research has attested to the importance of reading skill development by the end of third grade for later academic achievement. Against this background, we evaluated the effects of providing self-selected, developmentally appropriate books on the reading performance and summer reading amount of students in migrant schools in China, employing a randomized controlled trial. The intervention program improved rising second-graders’ word reading scores and summer reading amount. It proved particularly beneficial to low-performing children and children from disadvantaged families. The findings have implications for improving the academic achievement of migrant children in migrant schools in China and similar developing countries, especially where parental support may not be expected for the academic development of children.
Shear-Enhanced Stretchable Polymer Semiconducting Blends for Polymer-based Field-Effect Transistors
Qian-Yu Yan,Yu-Wei Shia,Dong-Yue Guo,Wen-Ya Lee 한국고분자학회 2020 Macromolecular Research Vol.28 No.7
Solution-sheared field-effect transistors based on the blend of the highmobility donor–acceptor conjugated copolymer poly(diketopyrrolo[3,4-c]pyrroleco- thieno[3,2-b]thiophene) (PDBT-co-TT) and the elastic polymer poly(styrene-butadienestyrene) (SBS) are demonstrated for stretchable electronics. In this study, PDBT-co- TT serves as a charge transport layer, and the insulating polymer SBS with double bonds is used for improving elasticity. Through combination with solution shearing, the phase separation and charge transport properties of the PDBT-co-TT/SBS blends can be manipulated. Compared with the spin-coated PDBT-co-TT/SBS blends showing lower charge mobilities (~10-3 cm2 V-1 s-1), the solution-sheared polymer-blend films with the PDBT-co-TT content of 20% maintain high mobility (>0.1cm2 V-1 s-1). The films with 60% PDBT-co-TT can even achieve mobility as high as 2 cm2 V-1 s-1, which is higher than the pristine conjugated polymer. Furthermore, as the SBS content increases, the dichroic ratios of the solution-sheared blends increase, indicating improved alignment of the conjugated polymer chains. The PDBT-co- TT/SBS blends exhibit great stretchability and high charge mobilities even under 100% strain due to their mesh-like morphology. Moreover, solution shearing not only improves polymer alignment but also controls surface morphology to enhance stretchability. This work reveals the importance of solution shearing in high-mobility stretchable polymer semiconductor blends.
( Qian Qian Li ),( Hong Ni ),( Shan Meng ),( Yan He ),( Zi Niu Yu ),( Lin Li ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.12
N-Acyl homoserine lactones (AHLs) serve as the vital quorum-sensing signals that regulate the virulence of the pathogenic bacterium Erwinia carotovora. In the present study, an approach to efficiently restrain the pathogenicity of E. carotovora-induced soft rot disease is described. Bacillus thuringiensis-derived N-acyl homoserine lactonase (AiiA) was projected onto the surface of Pseudomonas putida cells, and inoculation with both strains was challenged. The previously identified N-terminal moiety of the ice nucleation protein, InaQ-N, was applied as the anchoring motif. A surface display cassette with inaQ-N/ aiiA was constructed and expressed under the control of a constitutive promoter in P. putida AB92019. Surface localization of the fusion protein was confirmed by Western blot analysis, flow cytometry, and immunofluorescence microscopy. The antagonistic activity of P. putida MB116 expressing InaQ-N/AiiA toward E. carotovora ATCC25270 was evaluated by challenge inoculation in potato slices at different ratios. The results revealed a remarkable suppressing effect on E. carotovora infection. The active component was further analyzed using different cell fractions, and the cell surface-projected fusion protein was found to correspond to the suppressing effect.
Qian-Qian Wang,Li Cheng,Bi-Yu Wu,Hong-Yi Qiu,Ping Xu,Bo Wang,Xiu-Juan Yan,Sheng-Liang Chen 대한소화기 기능성질환∙운동학회 2023 Journal of Neurogastroenterology and Motility (JNM Vol.29 No.3
Background/AimsStigma related with antidepressants is prevalent in patients with functional dyspepsia. It affects medication compliance and efficacy. Herbal medicine acquired a deep-rooted cultural identity in relieving dyspeptic symptoms in Asians. The research was designed to compare the effectiveness of Zhizhu Kuanzhong capsules (ZZKZ) versus doxepin hydrochloride (doxepin) on alleviating stigma and medication nonadherence among patients with refractory FD (rFD). MethodsPatients with rFD from February 2021 to February 2022 were randomly allocated to receive either doxepin (n = 56) or ZZKZ (n = 57) combined with omeprazole for 4 weeks. Medication possession ratio (MPR), the disease- and medication-associated stigma were analyzed. The scales were utilized to assess dyspeptic symptoms (Leeds Dyspepsia Questionnaire) and psychological conditions (Generalized Anxiety Disorder Questionnaire and Patient Health Questionnaire). ResultsThe MPR values for ZZKZ were significantly higher than those for doxepin (P < 0.001). The stigma scores decreased in ZZKZ group while increased in doxepin group compared to baseline after treatment. The proportion of patients showing ZZKZ-associated stigma was significantly lower than doxepin-associated stigma (P < 0.001). The MPR values were negatively correlated with post-treatment stigma scores in both groups (P < 0.001). Dyspeptic symptoms and psychological condition were improved in both groups after treatment, with no significant difference on post-treatment Leeds Dyspepsia Questionnaire, Generalized Anxiety Disorder Questionnaire, or Patient Health Questionnaire scores between 2 groups. ConclusionZZKZ is superior to doxepin in alleviating stigma and medication non-adherence, with comparable efficacy in improving dyspeptic symptoms and psychological condition of patients with rFD.