Immobilization of Candida sp.99-125 lipase onto silanized SBA-15 mesoporous materials by physical adsorption

Ya Li,Pingfang Han,Wei Wang 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.1

SBA-15-NH2 and SBA-15-Cl Mesoporous Materials were prepared by modifying SBA-15 with silane coupling agent 3-aminopropyltriethoxysilane and 3-chloropropyltriethoxysilane using the post-synthesis method. The mesoporous samples were characterized by Fourier transform infrared spectra and nitrogen adsorption. Compared with SBA-15-NH2, SBA-15-Cl has suitable pore opening for further utilization in the immobilization of Candida sp.99-125 lipase by physical adsorption. The influences of lipase concentration and immobilizing time on the immobilization efficiency were investigated. Meanwhile, the lipase immobilized on SBA-15-Cl showed higher thermal, pH and storage stability than that of free lipase. Further study demonstrated that lipase immobilized on SBA-15-Cl could be used eight times without significant decrease of enzyme activity. The phenomenon was associated with the shrinkage of the pore opening of SBA-15-Cl keeping lipase from leaping out.

Comprehensive transcriptome analysis discovers novel candidate genes related to leaf color in a Lagerstroemia indica yellow leaf mutant

Ya Li,Zhenyu Zhang,Peng Wang,Shu’an Wang,Lingling Ma,Linfang Li,Rutong Yang,Yuzhu Ma,Qing Wang 한국유전학회 2015 Genes & Genomics Vol.37 No.10

Lagerstroemia indica is a popular woody ornamental plant throughout the world. However, relatively little is known about the molecular processes regulating leaf color in L. indica compared with other ornamental plants. Although yellow leaf mutants from various organisms have been well characterized, L. indica yellow leaf mutant has not yet been reported. In this study, a L. indica yellow leaf mutant, named YL03, was characterized and its leaf transcriptome was sequenced. A total of 30,712,752 reads were generated and assembled de novo into 45,308 unigenes with an average length of 987.51 bp. Among these unigenes, 21,339 (47.10 %) were identified as putative homologs of annotated sequences in public databases. A total of 79 unigenes involved in chlorophyll biosynthesis and degradation, photosynthesis and chloroplast development were identified. The expression levels of those genes were detected using quantitative real-time PCR in this study. Among those genes, 11 unigenes showed highly significant difference in the mutant compared to wild type plants. Conclusively, the leaf color formation is greatly affected by the activity of chloroplast development and chlorophyll metabolism. And the possible formation pathway of yellow leaf mutant is deduced based on our results.

Preparation, Structure and Performance of Poly(lactic acid)/Poly(lactic acid)-γ -Cyclodextrin Inclusion Complex-Poly(glycidyl methacrylate) Composites

Ya Li,Weijun Zhen 한국고분자학회 2018 Macromolecular Research Vol.26 No.3

Poly(lactic acid)-γ-cyclodextrin inclusion complex-poly(glycidyl methacrylate) (PLA-IC-PGMA) was synthesized with poly(lactic acid) (PLA) and γ-cyclodextrin inclusion complex via surface initiated atom transfer radical polymerization. Then, the PLA/PLA-IC-PGMA composite was prepared by melt blending using PLA-IC-PGMA as additive. Structure and properties of PLA-IC-PGMA and PLA/PLA-IC-PGMA composites were confirmed by X-ray diffraction patterns, 1H nuclear magnetic resonance spectroscopy, Fourier transform infrared spectra, X-ray photoelectron spectroscopy, thermogravimetric analysis, differential scanning calorimetry (DSC), polarized optical microscopy, rheological analysis, etc. Mechanical testing analysis showed that the tensile strength, elongation at break, and impact strength of PLA/ (1 wt%) PLAIC- PGMA composites were improved by 2.3%, 10.8%, and 52.5%, respectively, compared with pure PLA. Scanning electron microscopy analysis demonstrated that the toughness of PLA/PLA-IC-PGMA composite was improved. The results of DSC and TG showed that the cold crystallization peak of PLA composites after the addition of PLA-IC-PGMA disappeared, and its crystallinity was increased by 25% compared with PLA. Furthermore, the thermal stability of PLA/PLA-IC-PGMA composite was improved. Dynamic mechanical analysis showed that the glass transition temperature of PLA/PLA-IC-PGMA composites decreased by 8 oC, which indicated that the flexibility of the chain of PLA was greatly improved. Therefore, the addition of PLA-IC-PGMA improved the comprehensive performance of PLA.

Effects of Reaction Parameters on Self-degradation of L-ascorbic Acid and Self-degradation Kinetics

Ya Li,Yan Yang,Ai-Nong Yu,Kui Wang 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.1

The degradation behavior of L-ascorbic acid (ASA) was investigated under different parameters of temperature, time, and pH. Higher temperatures and longer times accelerated the ASA degradation. Degradation product distributions changed with different pH values. As solution pH of 4.5 was beneficial for formation of uncolored intermediate products with an absorbance maximum at 294 nm. Formation of brown products was promoted at pH values from 5.8 to 6.8 with an absorbance maximum at 420 nm. Under different pH conditions, volatile products formation varied. Furfural and derivatives of furan were primary products due to the effects of pH. The non-enzymatic selfdegradation behavior of ASA was characteristic of first-order kinetics based on a classic dynamic model. Activation energy values varied under different pH values. An ASA degradation mechanism and pathway are proposed.

Transcriptome analysis of the livers of ducklings hatched normally and with assistance

Yali Liu,Shishan He,Tao Zeng,Xue Du,Junda Shen,Ayong Zhao,Lizhi Lu 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.6

Objective: “Hatchability” is an important economic trait in domestic poultry. Studies on poultry hatchability focus mainly on the genetic background, egg quality, and incubation conditions, whereas the molecular mechanisms behind the phenomenon that some ducklings failed to break their eggshells are poorly understood. Methods: In this study, the transcriptional differences between the livers of normally hatched and assisted ducklings were systematically analyzed. Results: The results showed that the clean reads were de novo assembled into 161,804 and 159,083 unigenes (≥200-bp long) by using Trinity, with an average length of 1,206 bp and 882 bp, respectively. The defined criteria of the absolute value of log2 fold-change ≥1 and false discovery rate≤0.05 were differentially expressed and were significant. As a result, 1,629 unigenes were identified, the assisted ducklings showed 510 significantly upregulated and 1,119 significantly down-regulated unigenes. In general, the metabolic rate in the livers of the assisted ducklings was lower than that in the normal ducklings; however, compared to normal ducklings, glucose- 6-phosphatase and ATP synthase subunit alpha 1 associated with energy metabolism were significantly upregulated in the assisted group. The genes involved in immune defense such as major histocompatibility complex (MHC) class I antigen alpha chain and MHC class II beta chain 1 were downregulated in the assisted ducklings. Conclusion: These data provide abundant sequence resources for studying the functional genome of the livers in ducks and other poultry. In addition, our study provided insight into the molecular mechanism by which the phenomenon of weak embryos is regulated.

Optimal Photoperiod for Runner Induction in Strawberry ‘Seolhyang’ and ‘Maehyang’

Yali Li,Jiangtao Hu,Jie Xiao,Byoung Ryong Jeong 한국원예학회 2020 한국원예학회 학술발표요지 Vol.2020 No.11

Current Status and Prospect of Molecular Breeding in Tea Plant (Camellia sinensis)

(Yali Chang),(H. M. Prathibhani Chamidha Kumarihami),(Ho Bang Kim),(Kwan Jeong Song) 한국차학회 2017 한국차학회지 Vol.23 No.1

차는 건강 증진 효과는 물론 다양한 질병 예방 효과에 기인하여 세계적으로 가장 일반적으로 소비되는 비알코올 음료이며 많은 국가에서 중요한 소득원의 하나로서 과학 자와 생산자뿐만 아니라 소비자에게도 큰 관심의 대상이 되어 왔다. 그러므로 특정 이차대사산물의 고함유 또는 생물적 및 비생물적 스트레스의 저항성 증진과 같이 국제 차 시장에서 다양한 부가 가치를 줄 수 있는 새로운 차 품종의 육종이 시급히 요구되어 왔다. 전통 육종은 새로운 품종의 성공적인 개발에 매우 중요한 역할을 수행해 오고 있으나 육종 기간이 길고 비용이 많이 든다. 반면, DNA 분자표지의 개발은 육종 기간을 단축하고 차나무의 유전적 개량을 위한 육종 과정의 빠른 진행을 가능하게 해준다. 본 논문에서는 차나무의 유전적 다양성 및 유전분석, 핵심집단 및 유전자원 관리, 유전자 지도 및 분자표지 이용 선발, 그리고 유전체 및 유전자 발현 분석으로 구성하여 차나무 분자 육종과 유전적 개량의 최신 성과와 전망에 대해 전반적으로 고찰하고자 한다. Tea, as one of mostly popular consumed non-alcoholic beverages and an important revenue resource for a number of countries worldwide, has attracted a great deal of attention not only from scientists and growers, but also from consumers, because of its health-promoting function, as well as its protective effects against certain kinds of diseases. Therefore, there has been an enormous demand for breeding new tea cultivars with multiple value orientations in the international tea market, such as a high content of specific secondary metabolites or strong resistance to biotic and abiotic stresses. The conventional breeding process played an important role in the successful selection of new tea cultivars, but is time-consuming and costly. On the other hand, the discovery of DNA molecular markers has provided the opportunity to shorten the breeding time and accelerate the breeding process for improving the genetics of the tea plant. This paper is intended to provide comprehensive coverage of the recent achievements and perspectives in the field of tea plant molecular breeding and genetic improvement, integrated with genetic diversity and genotyping, core collection and genetic resources management, genetic linkage mapping and marker-assisted selection (MAS), as well as genomics and gene expression profiling.

Benzyladenine and Gibberellin Affect Runner Formation in Strawberry ‘Sulhyang’ and ‘Maehyang’

Yali Li,Jiangtao Hu,Hao Wei,Dong Il Kang,Yoo Gyeong Park,Byoung Ryong Jeong 한국원예학회 2019 한국원예학회 학술발표요지 Vol.2019 No.10

Modeling dynamic behavior of MDOF systems with multiple bilinear springs

Yali Ma,Shudong Yu,Delun Wang 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.9

A computational scheme is presented in this paper to simulate dynamical behavior of multiple degrees of freedom (MDOF) systems with multiple bilinear springs. In the proposed scheme, a bilinear spring is modeled using by two parallel springs - a primary spring and a secondary spring. The primary spring is an ordinary linear spring having identical stiffness in tension and compression, and is active for tension and compression. The secondary spring, whose stiffness characterizes the bilinearity, is active only during compression. It is employed in connection with the Newmark integration method and the linear complementarity problem (LCP) formulation to obtain time-domain responses of dynamical systems with bilinear springs due to initial disturbances and harmonic excitations. The scheme described in this paper is effective in dealing with the sudden transition from tension to compression and vice versa simultaneously for all bilinear springs. Numerical results for bilinear oscillators with finite bilinearity ratios and impact oscillators with an infinite bilinearity ratio show that the proposed bilinear spring model is accurate, generic and valid for bilinearity ratios ranging from zero to infinity. Orderly and chaotic behavior of viscously damped 3-DOF system under harmonic excitation is studied for a wide range of excitation frequencies and bilinear ratios to demonstrate the effectiveness and applicability of the proposed model for MDOF bilinear systems.

Epidemiology and resistance features of Acinetobacter baumannii isolates from the ward environment and patients in the burn ICU of a Chinese hospital

Yali Gong,Xiaodong Shen,Guangtao Huang,Cheng Zhang,Xiaoqiang Luo,Supeng Yin,Jing Wang,Fuquan Hu,Yizhi Peng,Ming Li 한국미생물학회 2016 The journal of microbiology Vol.54 No.8

Acinetobacter baumannii is an important opportunistic pathogen that causes severe nosocomial infections, especially in intensive care units (ICUs). Over the past decades, an everincreasing number of hospital outbreaks caused by A. baumannii have been reported worldwide. However, little attention has been directed toward the relationship between A. baumannii isolates from the ward environment and patients in the burn ICU. In this study, 88 A. baumannii isolates (26 from the ward environment and 62 from patients) were collected from the burn ICU of the Southwest Hospital in Chongqing, China, from July through December 2013. Antimicrobial susceptibility testing results showed that drug resistance was more severe in isolates from patients than from the ward environment, with all of the patient isolates being fully resistant to 10 out of 19 antimicrobials tested. Isolations from both the ward environment and patients possessed the β-lactamase genes blaOXA-51, blaOXA-23, blaAmpC, blaVIM, and blaPER. Using pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST), these isolates could be clustered into 4 major PFGE types and 4 main sequence types (ST368, ST369, ST195, and ST191) among which, ST368 was the dominant genotype. Epidemiologic and molecular typing data also revealed that a small-scale outbreak of A. baumannii infection was underway in the burn ICU of our hospital during the sampling period. These results suggest that dissemination of β-lactamase genes in the burn ICU might be closely associated with the high-level resistance of A. baumannii, and the ICU environment places these patients at a high risk for nosocomial infection. Cross-contamination should be an important concern in clinical activities to reduce hospital acquired infections caused by A. baumannii.