Robustness, tunability, and functional properties of topological surface states in topological insulator heterostructures

Zhenyu ZHANG 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Phosphoproteomic profiling of myofibrillar and sarcoplasmic proteins of muscle in response to salting

Zhang, Caixia,Wang, Zhenyu,Li, Zheng,Shen, Qingwu,Chen, Lijuan,Gao, Lingling,Zhang, Dequan 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

A phosphoproteomic profile of myofibrillar and sarcoplasmic proteins of muscle in response to salting was investigated. Myofibrillar and sarcoplasmic proteins extracted from salted meat with 0, 1, 2, 3, 4, and 5% salt for 0, 2, 4, 6, 8, and 16 h were analyzed by SDS-PAGE electrophoresis and fluorescence staining. The global phosphorylation of myofibrillar proteins in salted meat was lower than that in control muscle at 16 h of salting (p<0.05), and the global phosphorylation of myofibrillar proteins in 3% salt-treated group at 16 h was the lowest. However, salting showed no significant effect on phosphorylation of sarcoplasmic proteins. Four categories of phosphorylated protein were identified by LC-MS/MS, involved in stress response (heat shock protein), glycometabolism (glycogen phosphorylase, glyceraldehyde-3-phosphate dehydrogenase), oxidation or reduction (superoxide dismutase), and others (myoglobin), the phosphorylation of which was affected by salting. Thus, salting may influence meat quality through protein phosphorylation, which regulates protein degradation and glycolysis.

CXCL-13 Regulates Resistance to 5-Fluorouracil in Colorectal Cancer

Guolin Zhang,Xin Luo,Wei Zhang,Engeng Chen,Jianbin Xu,Fei Wang,Gaoyang Cao,Zhenyu Ju,Dongai Jin,Xuefeng Huang,Wei Zhou,Zhangfa Song 대한암학회 2020 Cancer Research and Treatment Vol.52 No.2

Purpose 5-Fluorouracil (5-Fu) is used as a conventional chemotherapy drug in chemotherapy for patients with advanced colorectal cancer, but many patients still suffer from treatment failure due to 5-Fu resistance. Emerging observations revealed the important role of chemokine (C-X-C motif) ligand 13 (CXCL-13) in tumor microenvironment and its relationship with prognosis in patients with colorectal cancer. This study is designed to reveal the important role of CXCL-13 in causing colorectal cancer resistance to 5-Fu. Materials and Methods CXCL-13 levels of patient's serum or cell culture supernatants were measured separately by enzyme-linked immunosorbent assay. In cell assays, cell viability is detected by Cell Counting Kit-8. Therefore, the recombinant human CXCL-13 was used to simulate its high expression in cells while its antibody and siRNA were used to reduce CXCL-13 expression in cells. Results In this study, we demonstrated that CXCL-13 is associated with 5-Fu resistance by culture medium exchange experiments and cytokine arrays of colorectal cancer resistant and nonresistant cells. Clinical studies showed that CXCL-13 is highly expressed in the serum of 5-Fu–resistant patients. High levels of serum CXCL-13 also predict a worse clinical outcome. The addition of recombinant CXCL-13 cytokine resulted in 5-Fu resistance, while its antibody overcame 5-Fu resistance, and knockdown of CXCL-13 expression by siRNA also reduced 5-Fu resistance, which can be saved by added recombination CXCL-13. Conclusion These results not only identify a CXCL-13 mediated 5-Fu resistance mechanism but also provide a novel target for 5-Fu–resistant colorectal cancer in prevention and treatment strategies.

Phosphoproteomic profiling of myofibrillar and sarcoplasmic proteins of muscle in response to salting

Caixia Zhang,Zhenyu Wang,Zheng Li,Qingwu Shen,Lijuan Chen,Lingling Gao,Dequan Zhang 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

A phosphoproteomic profile of myofibrillar and sarcoplasmic proteins of muscle inresponse to salting was investigated. Myofibrillar and sarcoplasmic proteins extracted from saltedmeat with 0, 1, 2, 3, 4, and 5% salt for 0, 2, 4, 6, 8, and 16 h were analyzed by SDS-PAGE electrophoresisand fluorescence staining. The global phosphorylation of myofibrillar proteins in salted meat was lowerthan that in control muscle at 16 h of salting (p<0.05), and the global phosphorylation of myofibrillarproteins in 3% salt-treated group at 16 h was the lowest. However, salting showed no significant effecton phosphorylation of sarcoplasmic proteins. Four categories of phosphorylated protein wereidentified by LC-MS/MS, involved in stress response (heat shock protein), glycometabolism (glycogenphosphorylase, glyceraldehyde-3-phosphate dehydrogenase), oxidation or reduction (superoxidedismutase), and others (myoglobin), the phosphorylation of which was affected by salting. Thus,salting may influence meat quality through protein phosphorylation, which regulates proteindegradation and glycolysis.

Short‑circuit fault detection scheme for DC microgrids on offshore platforms

Zhenyu Li,Huiran Sui,Ruifang Zhang,Guoling Wang,Huiyin Cai 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.5

DC microgrids present a very effective solution that enables the power systems of offshore platforms to achieve increased integration of renewable sources. Since the areas of offshore platforms are limited, the associated DC microgrids have lower line impedances, and short-circuit faults cause fault currents to rise rapidly. Thus, fault detection is a challenging issue due to the strict time limits for interruption imposed by these rapid rising fault currents. According to the fault characteristics and the ring structure of DC microgrids, this paper proposes a rapid detection scheme based on the differential current and current derivative without de-energizing the entire DC microgrid. It achieves rapid and selective fault detection and ensures an uninterruptible load power. The synchronization issues of the current differential and a rapid processing method for fault currents are investigated. The tripping threshold settings are discussed. Implementation of the fault detection scheme is also presented in detail. The proposed scheme is verified on a physical experimental platform. It is shown that here are some advantages such as good selectivity, low cost, and rapid fault detection. The scheme provides a strong guarantee for the uninterruptible operation of important equipment on offshore platforms.

Compressive resistance behavior of UHPFRC encased steel composite stub column

Zhenyu Huang,Xinxiong Huang,Weiwen Li,Jiasheng Zhang 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.37 No.2

To explore the feasibility of eliminating the longitudinal rebars and stirrups by using ultra-high-performance fiber reinforcement concrete (UHPFRC) in concrete encased steel composite stub column, compressive behavior of UHPFRC encased steel stub column has been experimentally investigated. Effect of concrete types (normal strength concrete, high strength concrete and UHPFRC), fiber fractions, and transverse reinforcement ratio on failure mode, ductility behavior and axial compressive resistance of composite columns have been quantified through axial compression tests. The experimental results show that concrete encased composite columns with NSC and HSC exhibit concrete crushing and spalling failure, respectively, while composite columns using UHPFRC exhibit concrete spitting and no concrete spalling is observed after failure. The incorporation of steel fiber as micro reinforcement significantly improves the concrete toughness, restrains the crack propagation and thus avoids the concrete spalling. No evidence of local buckling of rebars or yielding of stirrups has been detected in composite columns using UHPFRC. Steel fibers improve the bond strength between the concrete and, rebars and core shaped steel which contribute to the improvement of confining pressure on concrete. Three prediction models in Eurocode 4, AISC 360 and JGJ 138 and a proposed toughness index (T.I.) are employed to evaluate the compressive resistance and post peak ductility of the composite columns. It is found that all these three models predict close the compressive resistance of UHPFRC encased composite columns with/without the transverse reinforcement. UHPFRC encased composite columns can achieve a comparable level of ductility with the reinforced concrete (RC) columns using normal strength concrete. In terms of compressive resistance behavior, the feasibility of UHPFRC encased steel composite stub columns with lesser longitudinal reinforcement and stirrups has been verified in this study.

Proteomic Analysis of Goat Longissimus dorsi Muscles with Different Drip Loss Values Related to Meat Quality Traits

Zhenyu Wang,Fan He,Weili Rao,Na Ni,Qingwu Shen,Dequan Zhang 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.2

Longissimus dorsi muscles from 3 goat species were assigned to high and low drip loss groups. Physio-chemical properties, sarcomere length, and proteome profiles were investigated. The high drip loss group had lower pH, higher brightness, and higher shear force values, and shorter sarcomere lengths than the low drip loss group. 22 differential proteins were identified between high and low loss groups. α-Enolase, NADH dehydrogenase, pyruvate dehydrogenase E1, HSP27, superoxide dismutase, peroxiredoxin-2, myosin, and the myosin light chain were among these proteins, which were metabolic enzymes, stress response factors, and structural proteins that affected glycolysis, oxidation, and muscle contraction. Drip loss was probably produced via proteins involved in glycolysis, oxidation, and muscle contraction.

Identifying the geographical origin of protected sea cucumbers (Apostichopus japonicus) in China using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)

Zhenyu Yun,Zhao Sun,Haiyan Xu,Zhihong Sun,Yao Zhang,Zhen Liu 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.2

Dalian sea cucumber, Yantai sea cucumber, and Weihai sea cucumber, which belong to Apostichopus japonicus, are protected as geographical indications in China based on their high nutritional values and medical propertys. The 26 samples, including Dalian sea cucumbers (9 samples) in Liaoning province, Yantai sea cucumbers (9 samples), and Weihai sea cucumbers (8 samples) in Shandong province, were individually collected from the designated geographical sea areas and the genetic relationships and DNA polymorphisms were evaluated by random amplified polymorphic DNA technology and gene segments sequencing. The RAPD dendrogram showed that the genetic diversity of the three types of sea cucumbers was rich. The neighbor-joining tree shows that the genetic relationship of the samples from the adjacent sea areas is closer. It demonstrates that the gene characteristics of sea cucumbers from different sea areas were obvious and the genetic diversity analysis by RAPD-PCR can be used as a rapid method for geographical discrimination.

Antioxidant and Antigenotoxic Activities of Ethanol Extracts from Rhus chinensis Mill Leaves

Zhenyu Qiu,Mingli Tang,Guanjun Deng,Hao Yang,Xuan Zhang,Shengwei Huang,Lifang Wu 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.4

Ethanol extracts were obtained from Rhuschinensis Mill (RCM) leaves and used for antioxidant andantigenotoxic activity assays. IC50 values in DPPH assayswere 15.96, 18.83, 20.43, 27.93, 37.43, 46.21, and 141.84μg/mL for TPP, IPE, LLE, Vc, CE, BHT, and Trolox. Similar results were obtained using ABTS and FRAPassays. In vivo testing showed strong antioxidant activitiesthat were positively correlated with polyphenol contents. Leaf tissue contained abundant polyphenols, and more than10 phenolic compounds were detected in extracts. Quantitative results showed that quercetin-3-rhamnoside(26.4±0.76 mg/g of extract) was the most abundantingredient, followed by hyperoside (15.2±0.42 mg/g ofextract), quercetin (1.5±0.07mg/g of extract), and kaempferol(0.48±0.05 mg/g of extract). This study increases theknowledge for possible uses of forest by-products as asubstitute for gallnuts.

Gastric Plexiform Fibromyxoma with Two Different Growth Patterns on Histological Images: a Case Report

Li, Zhenyu,Jiang, Qingming,Guo, Dongfang,Peng, Yangling,Zhang, Jing,Chen, Xinyu The Korean Gastric Cancer Association 2021 Journal of gastric cancer Vol.21 No.2

Plexiform fibromyxoma (PF) of the stomach is a very rare mesenchymal tumor of the gastrointestinal tract. We report the first case of PF with 2 different growth patterns pathologically confirmed after surgical resection. The tumor was characterized microscopically as infiltrative; it demonstrated diffuse growth into the smooth muscle bundles of the muscularis propria and was also multinodular and plexiform within the myxoid stroma. Immunohistochemical analysis revealed that the tumor cells were positive or weakly positive for smooth muscle actin, vimentin, and H-caldesmon and negative for desmin, CD117, CD34, CK-20, Pan-CK, Dog1, S100, ER, PR, and CD10. No mutations of C-kit and platelet-derived growth factor receptor alpha were detected. No genetic disruption of glioma-associated oncogene homolog 1 was detected by fluorescence in situ hybridization. The final diagnosis of PF was mainly based on the morphological and immunohistochemical findings.