Effects of Liquid - Pig - Manure on Antioxidant Activity in Winter Forage Crops

Yae-Jin Moon,Kwang-Min Cho,Sang-Bok Lee,Jung-Jun Lee,Sang-Kyun Cho,Kee-Jong Kim,Jae-Chul Chun 한국잡초학회 2011 한국잡초학회 별책(학술대회 초록집) Vol.31 No.2

Benproperine, an ARPC2 inhibitor, suppresses cancer cell migration and tumor metastasis

Yoon, Yae Jin,Han, Young-Min,Choi, Jiyeon,Lee, Yu-Jin,Yun, Jieun,Lee, Su-Kyung,Lee, Chang Woo,Kang, Jong Soon,Chi, Seung-Wook,Moon, Jeong Hee,Lee, Sangku,Han, Dong Cho,Kwon, Byoung-Mog Elsevier 2019 Biochemical pharmacology Vol.163 No.-

<P><B>Abstract</B></P> <P>Metastasis is the leading cause of cancer mortality and cancer cell migration is an essential stage of metastasis. We identified benproperine (Benp, a clinically used antitussive drug) as an inhibitor of cancer cell migration and an anti-metastatic agent. Benp selectively inhibited cancer cell migration and invasion, which also suppressed metastasis of cancer cells in animal models. Actin-related protein 2/3 complex subunit 2 (ARPC2) was identified as a molecular target of Benp by affinity column chromatography with Benp-tagged Sepharose beads. Benp bound directly to ARPC2 in cells, which was validated by pull-down assay using Benp-biotin and label-free biochemical methods such as the drug affinity responsive target stability (DARTS) and cellular thermal shift assay (CETSA). Benp inhibited Arp2/3 function, showing disruption of lamellipodial structure and inhibition of actin polymerization. Unlike Arp2/3 inhibitors, Benp selectively inhibited the migration of cancer cells but not normal cells. ARPC2-knockdown cancer cells showed defective cell migration and suppressed metastasis in an animal model. Therefore, ARPC2 is a potential target for anti-metastatic therapy, and Benp has the clinical potential to block metastasis. Furthermore, Benp is a useful agent for studying the functions of the Arp2/3 complex in cancer cell migration and metastasis.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2′-hydroxycinnamaldehyde inhibits cancer cell proliferation and tumor growth by targeting the pyruvate kinase M2

Yoon, Yae Jin,Kim, Young-Hwan,Jin, Yena,Chi, Seung-Wook,Moon, Jeong Hee,Han, Dong Cho,Kwon, Byoung-Mog Elsevier 2018 Cancer letters Vol.434 No.-

<P><B>Abstract</B></P> <P>It is reported that 2′-hydroxycinnamaldehyde (HCA), isolated from cinnamon, has anti-tumor effects through the modulation of multi-target molecules. In this study, we identified pyruvate kinase M2 (PKM2) as a direct target of HCA by use of biochemical methods including affinity chromatography, drug affinity responsive target stability, and cellular thermal shift assay. PKM2 is up-regulated in multiple cancer types and is considered as a potential target for cancer therapy. HCA binds directly to PKM2 and selectively decreases the phosphorylation of PKM2 at Tyr105, indicating a potential anti-proliferative effect on prostate cancer cells. As a PKM2 activator, HCA increases pyruvate kinase activity by promoting the tetrameric state of PKM2. However, HCA suppresses protein kinase activity of PKM2 by decreasing the phosphorylation at Tyr105. Moreover, this leads to a decrease of PKM2-mediated STAT3 phosphorylation at Tyr705 and a down-regulation of target genes, including MEK5 and cyclin D1. Furthermore, HCA suppresses tumor growth and the release of tumor extracellular vesicles <I>in vivo</I> by inhibiting the phosphorylation of PKM2. Collectively, our results suggest that HCA may be a potential anticancer agent targeting PKM2 in cancer progression.</P> <P><B>Highlights</B></P> <P> <UL> <LI> HCA inhibits proliferation of DU145 prostate cancer cells and suppresses their growth in a xenograft mouse model. </LI> <LI> HCA directly binds to PKM2 in cancer cells and tumor tissues. </LI> <LI> HCA increases pyruvate kinase activity by promoting the tetrameric state of PKM2. </LI> <LI> HCA suppresses the release of tumor extracellular vesicles (EV) by dephosphorylating PKM2. </LI> </UL> </P>

강제수영실험을 통한 산청목의 항우울효과

진병문(Jin, Byung-Moon),이길현(Lee, Gil-Hyun),현경예(Hyun, Kyung-Yae) 한국산학기술학회 2014 한국산학기술학회논문지 Vol.15 No.11

은행속에 속하는 산청목은 중국과 한국 북동부지역에 널리 분포하는 자생 식물로 전통적으로 항염증제로 사용되었으 며 현재까지 항우울에 대한 효능과 그에 대한 면역변화에 대한 연구는 미흡한 실정이다. 본 연구실에서는 100, 200, 그리고 400 mg/kg 농도의 산청목추출물을 실험쥐에게 경구투여를 실행하였으며 강제수영실험을 통하여 항우울효과를 평가 그리고 혈 중 코티솔, ACTH, 그리고 사이토카인의 농도를 측정하였다. 실험 결과 실험쥐에 경구투여한 산청목 투입량이 증가함에 따라 강제수영실험에서의 행동불능시간이 감소하였으며 수중공포에 의해 분비유도된 사이토카인 농도 또한 감소함을 볼 수 있었다. 특히 코티솔, IL-6 과 IL-1β농도가 산청목 투입군에서 유의하게 감소함을 알 수 있었다. 산청목 경구투여군에서의 부동시간의 감소와 전염증성 사이토카인의 저하를 종합하여 볼 때 산청목의 항염증 효능으로 인한 작용에 의하여 강제수영 실험에서 항우울효과가 발현했다고 간주된다. Acer tegmentosum Maxim (AT) is a species of the maple genus, which is native to North-Eastern China and Korea. Traditionally, AT has been already used for pain relief in Korea. On the other hand, its antidepressant-like activity and related molecular mechanisms is not completely understood. Using the Forced Swimming Test (FST), the effects of a subacute treatment with AT(100, 200, and 400 mg/kg, p.o.) on the immobility and FST-induced changes to the immune parameters, cortisol, ACTH, and cytokine, in rats were investigated. The tendency of immobility showed a dose-dependent decrease in FST. The levels of cortisol, IL-6 and IL-1β in the peripheral blood were increased significantly after FST exposure. Overall, these results suggest that AT treatment can decrease the immobility time and the release of pro-inflammatory cytokines in the FST, suggesting that the anti-inflammatory effects of AT might be involved in the antidepressant-like effect.

포르말린으로 유도된 안면통증에 대한 홍삼추출물의 항염증 효과

진병문(Jin, Byung-Moon),이민경(Lee, Min-Kyung),이준선(Lee, Jun-Seon),현경예(Hyun, Kyung-Yae) 한국산학기술학회 2014 한국산학기술학회논문지 Vol.15 No.9

본 연구는 건강보조제 및 약제로서 전신의 염증반응 및 여러 약리적 효능을 가지고 있는 홍삼이 국소적인 안면 통증 의 완화에 영향을 끼치는지에 관해 조사하였다. 실험동물은 4그룹으로 대조군(n=6)과 5% 포르말린 주입군(n=6), 5% 포르말 린+D.W. 주입군, 홍삼(4.5 ml)(n=6) 복용군으로 나누어 45분간 안면을 긁거나 문지르는 행위를 계수하여 비교하였다. 홍삼 복용군에서 포르말린 주입군에 비해 유의하게 긁거나 문지르는 행위가 줄어들었으며, 15분부터 통증완화효과가 증가되기 시작되어 30분까지 유의한 효과를 보였다. 홍삼투여군에 대한 단백질정량분석 (western blot)으로 통증유발기전에 연관되는 염증과 항산화, NO생성에 관련된 신호전달 단백질 p38 MAPK와 iNOS, Nrf2를 뇌, 연수에서 활성정도를 비교한 결과 감소하 는 경향을 확인할 수 있었다. 이상의 결과들은 홍삼이 전신적 약리효과 뿐만 아니라 국소적인 염증, 통증완화에도 효능이 있음 나타낸다. The effects of korean red ginseng (KRG) extracts on orifacial pain control in terms of the systemic inflammatory response and pharmacological effects as health supplements were investigated. The experimental group were divided into three groups, the control group (n=6), formalin (5%, 50 ㎕) injection group (n=6), and formalin (5%, 50 ㎕) injection added KRG administrated group (4.5 ml/kg, n=6). The KRG administrated group prior to the formalin injection significantly attenuated the behavioral response compared to that of the control group. Pain reduction was suppressed mainly from 15 min to 30 min. The KRG administrated rats showed significantly reduced p38 MAPK, iNOS and Nrf2 expression in the brain and medulla oblongata according to Western blot analysis. These findings suggest that KRE may have a useful effect on orificial pain control functions by preventing the p38 MAPK pathway.

다약재 내성 유전자 종류 규명

문예성(Yae-Sung Mun),고용훈(Yong-Hoon Go),이용현(Yong-Hyun Lee),이은혁(Eun-Hyuk Lee),강현아(Hyun-A Kang),김연재(Yun-Jae Kim),문송미(Song Mi Moon),황유진(You-Jin Hwang) 한국산학기술학회 2014 한국산학기술학회 학술대회 Vol.- No.-

다약재 내성을 나타내는 병원성 미생물에 의한 질병이 점점 많아지고 있다. 항생제 내성을 나타내는 유전자가 발현되어 항생제 내성을 나타내게 되고 저항성을 갖는다. 본 논문에서는 병원성 미생물인 methicillin-resistance Staphylococcus aureus (MRSA)가 다약재 내성을 나타내고 있는 것을 확인하고 다약재 내성을 나타나게 하는 유전자의 종류를 규명하고자 하였다.

기 취득 데이터와 센서 구성이 다른 자율주행차의 개발을 위한 센서 데이터 변환 기술

박진만(Jin-Man Park),예해주(Haejoo Yae),문병일(Byeong-Il Moon),최영권(Yeongkwon Choe),손행선(Hang-Seon Son) 대한전자공학회 2022 대한전자공학회 학술대회 Vol.2022 No.6

The Ameliorative Effects of Korean Bean‐Leaves on Inflammation and Liver Injury in Obese Rat Model

Byung?Moon Jin,Seok?Cheol Choi,Hye?Sook Lee,Sang?Bong Jung,Kyung?Yae Hyun 대한의생명과학회 2013 Biomedical Science Letters Vol.19 No.3

Obesity may cause metabolic syndrome and adult diseases. This study was undertaken to investigate the ameliorative or useful effects of beanleaves on inflammation and liver damage in obese rat models. Rats were divided into three groups: a control group (normal diet, n=6), a fat diet group (45%?fat diet, n=7), and a bean leaf group (45%?fat+Korean bean leaves diet, n=7). Body weights in the bean leaf group were lower than those of the fat group (P<0.05). Serum tumor necrosis factor?α (TNF?α) and prostaglandin E₂ (PGE₂) concentrations were lower in both the control and bean leaf groups than in the fat group (P<0.001). TNF?α concentrations in the bean leaf group were slightly higher than in the control group but statistically significant (P<0.05). The bean leaf group histologically exhibited lower fatty degeneration, spotty necrosis, and leukocyte infiltrations in hepatic tissues than those of the fat group. In the homogenized liver tissues, the cyclooxygenase?2 (COX?2) gene was only expressed in the fat group. The gene expression levels of hepatic TNF?α, inducible nitric?oxide synthase, peroxiome proliferator?activated receptor?α (PPAR?α), poly (ADP?ribose) polymerase (PARP), and transforming growth factor?β1 (TGF?β1) were weaker in the bean leaf group than in the fat group. These results suggest that adding bean?leaves to the diet may ameliorate obesity?induced systemic inflammation and liver damage and that bean leaves may be a useful food for preventing obesity and thereby metabolic syndrome and adult diseases

Effect of the Resveratrol and Sulforaphane Compound on the Regulation of Orofacial Pain

Kyung-Yae Hyun,Min-Kyung Lee,Gil-Hyun Lee,Byung-Moon Jin 한국실험동물학회 2017 한국실험동물학회 학술발표대회 논문집 Vol.2017 No.8

미술치료사의 발달장애 아동 대상 집단치료 경험에 대한 현상학적 연구

허예진(Heo, Yae-Jin),문경아(Moon, Kyung-Ah) 한국예술심리치료학회 2019 예술심리치료연구 Vol.14 No.4

이 연구는 미술치료사들의 발달장애 아동 대상 집단치료 수행경험을 고찰하는데 목적이 있다. 이를 위하여 연구자는 7명의 미술치료사를 대상으로 그들의 발달장애 아동 대상 집단 치료 경험에 대한 심층면담을 진행한 뒤, Giorgi(2009)의 현상학적 연구 방법을 통하여 결 과를 도출하였다. 그 결과 ‘긍정적 요인’, ‘ 구조적 어려움’, ‘전문적 어려움’, ‘치료적 개입 요 인’, ‘어려움 개선 요인’, ‘치료사의 성장 경험’ 총 6가지의 구성요소와 15개의 하위 구성요소 가 도출되었다. 발달장애 아동 대상 집단치료 수행 과정에서 미술치료사들은 집단이 지닌 힘을 목격하였다. 아울러 발달장애 아동들로 구성된 집단미술치료 과정에서 발생하는 다양 한 어려움을 극복하기 위하여 지식 습득, 피어비전 등 개인적 노력과, 사회·제도적 지원 체 계의 중요성 또한 인식할 수 있었다. 이와 같이 발달장애 아동 대상 집단치료 수행 경험을 통하여 미술치료사들은 집단치료 중재 기술 향상과 발달장애에 대한 깊은 이해 및 잠재력 을 발견하는 등 보다 나은 치료사로 성장할 수 있었다. 이 연구는 미술치료사의 발달장애 아동 대상 집단치료 경험을 심층적으로 고찰했다는 것에 의의가 있으며, 향후 집단미술치료 현장에 대한 기초자료로 제공하고자 한다. This study aims to carefully examine the experiences of art therapists as noted in the group therapy of children with developmental disabilities. For this purpose, the researcher conducted in-depth interviews with seven art therapists about their experiences on the group therapy of children with developmental disabilities. After that each interview was analyzed using the phenomenological method of Amedeo Giorgi(2009). The results include 15 sub-categories and 6 components, which included: positive factor, structural difficulty, professional difficult, therapeutic intervention factor, factor for improving difficulty, and professional development. In this sense, the art therapist had witnessed the therapeutic power of group while performing group therapy on children with developmental disabilities and recognized that group therapy should be facilitative environment where clients can function entirely. Meanwhile, art therapists had experienced group dynamics, art intervention and structural difficulties due to children with developmental disabilities in groups in the process of group therapy. To overcome such difficulties, they had made various efforts and have grown professionally using these techniques. Finally, this study is meaningful in that it explored experiences of group therapy of children with developmental disabilities by art therapists in depth, and aims to provide this as fundamental data on the fields of group art therapy on children with developmental disabilities.