Human Papillomavirus (HPV) Infection in Women Participating in Cervical Cancer Screening from 2006 to 2010 in Shenzhen City, South China

Wang, Yue-Yun,Li, Li,Wei, Sheng,Peng, Ji,Yuan, Shi-Xin,Xie, Jian-Sheng,Liu, Zhi-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Purpose: Human papillomavirus (HPV) infection plays an important role in the development of cervical cancer, but the prevalence of HPV infection in women of Shenzhen city remains unclear. The present study was performed to describe the change of cervical HPV infection in females who participated in voluntary cervical cancer screening from 2006 to 2010 in Shenzhen city, China. Methods: A total of 4, 413 women were recruited. HPV infections were genotyped by polymerase chain reaction (PCR) and reversed dot blot hybridization in Shenzhen Maternity and Child Health Hospital. Results: The prevalence of HPV infection was 13.8%. The five most commonly found HPV types were HPV16 (3.47%), HPV58 (1.68%), HPV33 (1.38%), HPV43 (1.36%) and HPV18 (1.27%). The secular trends of major HPV type-specific were diverse. Among of them, the prevalence of HPV18 increased sharply while others increased slowly or even decreased in the period. The change of total HPV, single HPV and multiple HPV infection were similar during the five years. Conclusions: Our findings suggested that HPV infection is common with HPV16 and HPV 58 as the primary subtypes in women in Shenzhen city.The prevalence of HPV 18 infection is increasing faster than any others, which will lead it to be one of the main subtypes in this city in the future.

Methods for distinguishing Mott transitions from Anderson transitions

Wang, Yue,Kang, Kyung Mun,Kim, Minjae,Yoo, Hyang Keun,Park, Hyung Ho Inderscience Enterprises Ltd. 2018 International Journal of Nanotechnology Vol.15 No.6

Systematic polymorphism analysis of the CYP2C9 gene in Chinese Han and Tibetan populations

Yue-Wen Wang,Xiao-Hong Meng,Wei Yan,Xiao-Qing Zhang,Jin Yang 한국유전학회 2012 Genes & Genomics Vol.34 No.3

Study about polymorphism of the CYP2C9 was not reported in the Chinese Tibetan population and there was no comparison of genetic polymorphism pattern of CYP2C9 between Chinese Han and Tibetan populations. Here we screened the genetic polymorphisms of functional regions of the CYP2C9in 100 unrelated healthy Chinese Han and Tibetan volunteers,respectively, using direct sequencing. A total of 20 variants were detected and there were different distribution of allelic and genotype frequencies, linkage disequilibrium patterns,haplotype structures and htSNPs between the two populations. CYP2C9*3 is a major functional variant of CYP2C9 in the two populations and *11 allele was only detected in Tibetan population. The determined genetic information of CYP2C9in Chinese Han and Tibetan populations might serve as a baseline for larger studies on determining metabolic phenotypes of CYP2C9 substrate drugs and also provide important data for the advance of personalized medicine in Chinese Han and Tibetan populations.

Research on Phytotoxicity of Natural Colloids

Yue Wang,Xiangang Hu 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-

Proteomic Comparison between Salmonella Typhimurium and Salmonella Typhi

Yue Wang,Kuan-Yeh Huang,Yanan Huo 한국미생물학회 2014 The journal of microbiology Vol.52 No.1

The genus Salmonella contains more than 2500 serovars. While most cause the self-limiting gastroenteritis, a few serovars can elicit typhoid fever, a severe systemic infection. S. enterica subsp. enterica serovar Typhimurium and S. Typhi are the representatives of the gastroenteritis and typhoid fever types of Salmonella. In this study, we adopted Stable Isotope Labeling with Amino acids in Cell culture (SILAC) technology to quantitatively compare the proteomes of the two serovars. We found several proteins with serovar-specific expression, which could be developed as new biomarkers for clinical serotype diagnosis. We found that flagella and chemotaxis genes were down-regulated in S. Typhi in comparison with S. Typhimurium. We attributed this observation to the fact that the smooth cellular structure of S. Typhi may better fit its systemic lifestyle. Instead of known virulence factors that were located within Salmonella Pathogenecity Islands, a number of core genes, which were involved in metabolism and transport of carbohydrates and amino acids, showed differential expression between the two serovars. Further studies on the roles of these differentially-expressed genes in the pathogenesis should be undertaken.

Low temperature method to passivate oxygen vacancies in un-doped ZnO films using atomic layer deposition

Wang, Yue,Kang, Kyung-Mun,Kim, Minjae,Park, Hyung-Ho Elsevier 2018 THIN SOLID FILMS - Vol.660 No.-

<P><B>Abstract</B></P> <P>Owing to oxygen vacancies, the as-prepared ZnO normally shows n-type semiconducting characteristic. This has restricted the preparation of high-quality p-type ZnO and the application of ZnO optoelectronic devices. Therefore, we studied a method of using H<SUB>2</SUB>O<SUB>2</SUB> as an oxygen source to passivate oxygen vacancies (V<SUB>o</SUB>) in ZnO films via atomic layer deposition (ALD). The temperature range for the self-limited growth of crystalline ZnO thin films by ALD using diethylzinc and H<SUB>2</SUB>O<SUB>2</SUB> was found to be in the range of 80 to 150 °C. Our results show that the use of H<SUB>2</SUB>O<SUB>2</SUB> as an oxygen source can provide an O-rich condition (instead of H<SUB>2</SUB>O) for the growth of ZnO film, with a total preferential (002) orientation of the growth plane and decreased grain size. Further, the O-rich growth environment can suppress the formation of V<SUB>o</SUB> and zinc interstitials and decrease the carrier concentration in ZnO (from 2.525 × 10<SUP>19</SUP> cm<SUP>−3</SUP> to 1.695 × 10<SUP>12</SUP> cm<SUP>−3</SUP>). This can lead to an increase in the film resistivity from 1.717 × 10<SUP>−2</SUP> Ω·cm for a ZnO film prepared using H<SUB>2</SUB>O to 1.348 × 10<SUP>4</SUP> Ω·cm for a ZnO film prepared using H<SUB>2</SUB>O<SUB>2</SUB>. Thus, H<SUB>2</SUB>O<SUB>2</SUB> could be used to passivate V<SUB>o</SUB> in ZnO at a low temperature, and it could be beneficial for the preparation of p-type ZnO films.</P> <P><B>Highlights</B></P> <P> <UL> <LI> H<SUB>2</SUB>O<SUB>2</SUB> was proved to provide more oxygen than H<SUB>2</SUB>O for ALD growth of ZnO film. </LI> <LI> The oxygen-rich condition of H<SUB>2</SUB>O<SUB>2</SUB> can passivate the oxygen vacancy in ZnO film. </LI> <LI> Vacancy decrease leads to carrier concentration decrease and resistivity increase. </LI> <LI> H<SUB>2</SUB>O<SUB>2</SUB> passivates oxygen vacancy efficiently at low temperature than high temperature. </LI> </UL> </P>

Implementing a Consistency Checker for Uncertain or Incomplete Temporal System

Yue Wang,Jixin Ma,Brian Knight 보안공학연구지원센터 2013 International Journal of Hybrid Information Techno Vol.6 No.6

Taxol Produced from Endophytic Fungi Induces Apoptosis in Human Breast, Cervical and Ovarian Cancer Cells

Wang, Xin,Wang, Chao,Sun, Yu-Ting,Sun, Chuan-Zhen,Zhang, Yue,Wang, Xiao-Hua,Zhao, Kai Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1

Currently, taxol is mainly extracted from the bark of yews; however, this method can not meet its increasing demand on the market because yews grow very slowly and are a rare and endangered species belonging to first-level conservation plants. Recently, increasing efforts have been made to develop alternative means of taxol production; microbe fermentation would be a very promising method to increase the production scale of taxol. To determine the activities of the taxol extracted from endophytic fungus N. sylviforme HDFS4-26 in inhibiting the growth and causing the apoptosis of cancer cells, on comparison with the taxol extracted from the bark of yew, we used cellular morphology, cell counting kit (CCK-8) assay, staining (HO33258/PI and Giemsa), DNA agarose gel electrophoresis and flow cytometry (FCM) analyses to determine the apoptosis status of breast cancer MCF-7 cells, cervical cancer HeLa cells and ovarian cancer HO8910 cells. Our results showed that the fungal taxol inhibited the growth of MCF-7, HeLa and HO8910 cells in a dose-and time-dependent manner. IC50 values of fungal taxol for HeLa, MCF-7 and HO8910 cells were $0.1-1.0{\mu}g/ml$, $0.001-0.01{\mu}g/ml$ and $0.01-0.1{\mu}g/ml$, respectively. The fungal taxol induced these tumor cells to undergo apoptosis with typical apoptotic characteristics, including morphological changes for chromatin condensation, chromatin crescent formation, nucleus fragmentation, apoptotic body formation and G2/M cell cycle arrest. The fungal taxol at the $0.01-1.0{\mu}g/ml$ had significant effects of inducing apoptosis between 24-48 h, which was the same as that of taxol extracted from yews. This study offers important information and a new resource for the production of an important anticancer drug by endofungus fermentation.

A native Trichoderma harzianum strain Th62 displays antagonistic activities against phytopathogenic fungi and promotes the growth of Celosia cristata

Yue-Feng Wang,Xue-Yue Hou,Chuan-Ying Jiang,Tong-Tong Zhai,Rui Miao,Jun-Jie Deng,Zhi-Hong Yao,Rongshu Zhang 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.2

Trichoderma spp. are widely applied, eco-friendly mycofungicides and plant growth promoters. Native Trichoderma strainsare likely to have more productive, stable biocontrol and biofertilizer activities since they are well adapted to the local environment. In this study, we isolated a native Trichoderma strain ‘Th62’ from the rhizosphere soil of wild Chelidonium majusplants in Harbin, China (126.6341°E, 45.7242°N). The isolated Trichoderma strain was identifi ed as a T. harzianum strainvia morphological observation and molecular methods based on the rDNA internal transcribed spacer region and elongationfactor-1α gene sequences. Signifi cant antagonistic activities of Th62 against fi ve soil-borne fungal phytopathogens,Fusarium oxysporum , Sclerotinia sclerotiorum , Alternaria alternata , Cytospora chrysosperma, and Rhizoctonia solani ,were confi rmed by dual-culture assays. Furthermore, the crude fermentation products of Th62 also displayed antifungalactivities against these fi ve pathogens. To evaluate the function of Th62 as a biofertilizer, we subsequently applied Th62on cockscomb ( Celosia cristata L), a plant species with both ornamental and medicinal values, by inoculation with Th62conidia at diff erent concentrations, 1 × 10 10 cfu mL −1 , 1 × 10 11 cfu mL −1 , and 1 × 10 12 cfu mL −1 . The benefi cial eff ects ofTh62 were evaluated by measuring the growth and photosynthetic traits of the inoculated cockscomb plants, and the resultsdemonstrated that Th62 signifi cantly improved the photosynthetic effi ciency, photosynthetic capacity, and the adaptabilityto intense light of the inoculated cockscomb plants compared to the controls. Consistently, Th62 inoculation signifi cantlyimproved the growth and fl ower yield of cockscomb. We presented a positive case of isolating and applying native microbialresources on local plantation practices.

Nicotinamide suppresses cell growth by G1-phase arrest and induces apoptosis in intrahepatic cholangiocarcinoma

Yue Wang,Han Suk Ryu,Ja-June Jang 대한독성 유전단백체 학회 2018 Molecular & cellular toxicology Vol.14 No.1

Intrahepatic cholangiocarcinoma (iCCA) is a devastating malignancy with no effective treatment. Nicotinamide (NA, the amide form of vitamin B3) has been shown to be effective in the treatment of various diseases. However, the effects of NA in iCCA have not been studied. In this study, four human iCCA cell lines(HuCCT1, JCK, OZ and Cho-CK) were used. We found that NA significantly inhibited cell viability and induced apoptosis in vitro. It arrested cell cycle in G1 phase, decreased Cyclin D1 and Cdk4 protein expression levels and increased p16 level. NA increased the levels of cleaved caspases 3 and 9, but had no effect on caspase 8. In HuCCT1 and OZ cell lines, NA treatment significantly impaired the invasion abilities and supressed epithelial-mesenchymal transition (EMT)- like changes. In conclusion, our findings provide the experimental basis for using NA as a potential anticancer agent against human iCCA in the future.