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In vitro single-vessel enzymatic synthesis of novel Resvera-A glucosides
Shin, J.Y.,Pandey, R.P.,Jung, H.Y.,Chu, L.L.,Park, Y.I.,Sohng, J.K. Elsevier Scientific Pub 2016 Carbohydrate research Vol.424 No.-
<P>An in vitro enzymatic glycosylation system is developed for the efficient synthesis of glucosides of 3,5-dihydroxy-N-(4-hydroxyphenyl) benzamide (resvera-A), a chemically synthesized molecule resembling resveratrol in structure. Resvera-A is a pharamacophore-based designed molecule that exhibits antioxidant, antibacterial, anti-inflammatory, and anticancer activities. In this study, an alternative cost-effective uridine diphosphate (UDP) recycling system was established to produce UDP-alpha-D-glucose through a two-step enzyme-catalyzed reaction using easily available cheap sources. This UDP-alpha-D-glucose biosynthesis system was combined with a glycosyltransferase (YjiC, from Bacillus licheniformis)-catalyzed reaction for the synthesis of glucoside derivatives of resvera-A. The side product of the glycosylation reaction, UDP, was used as a precursor for the biosynthesis of UDP-alpha-D-glucose, which is used by YjiC for glycosylation, thus recycling UDP. As a result, two novel molecules, resvera-A 3-O-alpha-D-glucoside (42.33 mg, 2.10 mM, 0.84 mg/mL) and resvera-A 4'-O-alpha-D-glucoside (99.38 mg, 4.87 mM, 1.98 mg/mL), were synthesized within 4 h from 50 mL preparative scale reaction using only 0.1 mM of UDP-alpha-D-glucose, 100 folds lower concentration than the concentration of resvera-A (10 mM) used. Structures of both products were elucidated using liquid chromatography, mass spectroscopy, and nuclear magnetic resonance analysis. (C) 2016 Elsevier Ltd. All rights reserved.</P>
Y. G. Bala,S. Raman Sankaranarayanan,K. S. Pandey 대한금속·재료학회 2015 METALS AND MATERIALS International Vol.21 No.6
The present investigation was carried out to evaluate the densification, mechanical properties, microstructural and fractrography effects of AISI 8630 steel composition developed through powder preform forging under different heat treated conditions. Sintered preforms of different aspect ratios such as 0.6, 0.9, and 1.2 were hot upset forged to disc shape to different height strain to analysis the densification mechanism. Certain relationships relating strains, Poisson’s ratio relating densification have revealed the effect of preform geometry on densification kinetics and resulted in the polynomial expression with justified regression coefficient greater the 0.9 or unity. The preforms of aspect ratio of 1.1 were hot upset forged to square cross section bars and transferred to different quenching medium like oil, water, furnace and air to assess its mechanical properties. Comparing the temperament of the heat treatments, sintered forged homogenised water quenched sample upshot in the maximum Tensile strength with least per centage elongation andthe furnace cooled sample shows the maximum toughness with desirable per centage elongation and least tensile strength. Microstructure stated the presence of varying ferrite and pearlite distribution and fractograph studies has disclosed the mixed mode of failure on the effect of varying heat treatments progression has affected the properties significantly.
Biosynthesis of a novel fisetin glycoside from engineered Escherichia coli
Pandey, R.P.,Parajuli, P.,Chu, L.L.,Kim, S.Y.,Sohng, J.K. Korean Society of Industrial and Engineering Chemi 2016 Journal of industrial and engineering chemistry Vol.43 No.-
<P>Escherichia coli BL21(DE3)/Delta pgi Delta zwf Delta galU mutant was engineered by overexpressing thymidine diphosphate (dTDP)-D-glucose synthase (tgs), dTDP-D-glucose 4,6-dehydratase (dh), and a sugar aminotransferase (wecE) from different sources to produce a pool of dTDP-4-amino-4,6-dideoxy-D-galactose in the cell cytosol. To this recombinant mutant, two Arabidopsis thaliana glycosyltransferases (ArGT-3 and ArGT-4) were overexpressed to generate two glycosylation platforms (E. coli BL21(DE3)/ Delta pgi Delta zwf Delta galUTDW-3 and E. coli BL21(DE3)/Delta pgi Delta zwf Delta galUTDW-4), which were accessed for the glycosylation of fisetin. As a result, one of the two systems, E. coli BL21(DE3)/Delta pgi Delta zwf Delta galUTDW-3, was able to conjugate 4-amino-4,6-dideoxy-D-galactose sugar at the 3-OH position of fisetin, producing an unnatural fisetin 3-O-4-amino-4,6-dideoxy-D-galactoside. (C) 2016 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.</P>
Kim, K.H.,Pandey, S.K.,Kim, Y.H.,Sohn, J.R.,Oh, J.M. Academic Press 2015 Ecotoxicology and environmental safety Vol.114 No.-
The emission rates of N,N-dimethylformamide (DMF), formamide (FAd), and certain hazardous volatile organic compounds (VOCs) were measured from seventeen mattress textile samples with four different raw material types: polyurethane (PU: n=3), polyester/polyethylene (PE: n=7), ethylene vinyl acetate (EV: n=3), and polyvinyl chloride (PC: n=4). To simulate the emissions in a heated room during winter season, measurements were made under temperature-controlled conditions, i.e., 50<SUP>o</SUP>C by using a mini-chamber system made of a midget impinger. Comparison of the data indicates that the patterns were greatly distinguished between DMF and FAd. PU products yielded the highest mean emission rates of DMF (2940μgm<SUP>-2</SUP>h<SUP>-1</SUP>: n=3) followed by PE (325μgm<SUP>-2</SUP>h<SUP>-1</SUP>: n=7), although its emission was not seen from other materials (EV and PC). In contrast, the pattern of FAd emission was moderately reversed from that of DMF: EV>PC>PE>PU. The results of our analysis confirm that most materials used for mattress production have the strong potential to emit either DMF or FAd in relatively large quantities while in use in children@?s care facilities, especially in winter months. Moreover, it was also observed that an increase in temperature (25<SUP>o</SUP>C to 50<SUP>o</SUP>C) had a significant impact on the emission rate of FAd and other hazardous VOCs. In addition to the aforementioned amides, the study revealed significant emissions of a number of hazardous VOCs, such as aromatic and carbonyl compounds.
Evaluation of Genetic Variation and Phylogenetic Relationship among North Indian Cattle Breeds
Sharma, Rekha,Pandey, A.K.,Singh, Y.,Prakash, B.,Mishra, B.P.,Kathiravan, P.,Singh, P.K.,Singh, G. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.1
In the present study, genetic analyses of diversity and differentiation were performed on four breeds of Indian zebu cattle (Bos indicus). In total, 181 animals belonging to Ponwar, Kherigarh, Gangatiri and Kenkatha breeds were genotyped for 20 cattle specific microsatellite markers. Mean number of alleles observed per locus (MNA) varied between 5.75 (Kenkatha) to 6.05 (Kherigarh). The observed and expected heterozygosity for the breeds varied from 0.48 (Gangatiri) to 0.58 (Kherigarh) and 0.65 (Kenkatha) to 0.70 (Kherigarh), respectively. $F_{IS}$ estimates of all the breeds indicated significant deficit of heterozygotes being 28.8%, 25.9%, 17.7% and 17.7% for Gangatiri, Ponwar, Kherigarh and Kenkatha, respectively. The $F_{ST}$ estimates demonstrated that 10.6% was the average genetic differentiation among the breeds. Nei's genetic distance DA and Cavalli- Sforza and Edwards Chord distance ($D_C$) and the phylogenetic tree constructed from these reflected the close genetic relationship of Gangatiri and Kenkatha, whereas Ponwar appears to be more distant.
Regmi, C.,Kshetri, Y.K.,Ray, S.K.,Pandey, R.P.,Lee, S.W. Elsevier BV * North-Holland 2017 Applied Surface Science Vol.392 No.-
Lanthanide-doped BiVO<SUB>4</SUB> semiconductors with efficient photocatalytic activities over a broad range of the solar light spectrum have been synthesized by the microwave hydrothermal method using ethylenediaminetetraacetic acid (EDTA). The structural, morphological, and optical properties of the as-synthesized samples were evaluated by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray powder diffraction (XRD), Raman spectroscopy, FT-IR spectroscopy, UV-vis diffuse reflectance spectroscopy (DRS), and photoluminescence spectroscopy (PL). The chemical compositions were analyzed by X-ray photoelectron spectroscopy (XPS). The toxicity of the samples was measured using Mus musculus skin melanoma cells (B16-F10 (ATCC<SUP>®</SUP> CRL-6475(TM))) and were found to be nontoxic for human cells. The photocatalytic efficiency of the prepared samples was evaluated by methylene blue (MB) degradation. The best photocatalytic activity was shown by BiVO<SUB>4</SUB> with 6:3:3mol percentage of Yb<SUP>+3</SUP>:Er<SUP>+3</SUP>:Tm<SUP>+3</SUP> in all solar light spectrum. The synthesized samples possess low band gap energy and a hollow structure suitable for the better photocatalytic activity. The observed NIR photoactivity supports that the upconversion mechanism is involved in the overall photocatalytic process. Therefore, this approach provides a better alternative upconversion material for integral solar light absorption.