유착에 의한 AGS 및 Hep-G2 세포 표면 구조의 변화

박동규 ( D. K. Park ),전훈재 ( H. J. Chun ),박재홍 ( J. H. Park ),박철희 ( C. H. Park ),진윤태 ( Y. T. Jeen ),이홍식 ( H. S. Lee ),이상우 ( S. W. Lee ),엄순호 ( S. H. Um ),최재현 ( J. H. Choi ),김창덕 ( C. D. Kim ),류호상 ( H. S. Ryu 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-

<목적> 최근 H. pylori 유착에 의한 세포 표면 구조의 변화에 관한 연구가 시도되어지고 있으나 actin 의 변화여부 및 그 특성에 관해서는 아직 명확히 정립되지 못한 실정이다. Rho GTPase는 세포 표면의 미세돌기인 microvilli, filopodia 및 membrane ruffle의 형성과 관련이 있으며, 최근 AGS 세포에서 H. pylori가 Rac activation에 의하여 membrane ruffle을 형성한다는 것과 Rac

패스틴^� 첨가가 단백질 분해율과 반추위 발효 및 영양소 소화율에 미치는 영향

최유지,최낙진,박성호,송재용,엄재상,고종렬,하종규 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.5

본 시험은 패스틴^R을 첨가하였을 때, in vitro 상에서 단백질 fraction과 분해율에 미치는 영향과, in vivo 상에서 반추위 성상, 미생물 군집, 암모니아태 질소 농도 및 영양소 소화율에 미치는 영향을 규명하고자 실시하였다. In vitro 실험에서는 1㎜로 분쇄된 대두박을 기질로 하여 패스틴^R(㈜은진인터내셔날)을 첨가하여 borate-phosphate buffer와 중성세제에서의 조단백질 분해율을 측정하였으며, exogenous enzyme (Streptomyces griseus 유래 protease)를 이용하여 39℃에서 0, 2, 4, 8, 12, 48 시간동안 배양 후 조단백질 분해율을 측정하였다. 반추위 발효성상과 영양소 소화율은 반추위 fistula가 부착된 평균체중 300㎏의 홀스타인 수소 4두를 이용하여 무첨가구, 패스틴^R 첨가구의 두 개 처리구에 2마리씩 4마리를 배치하여 측정하였다. Buffer Soluble Protein fraction은 패스틴^R 첨가 수준별로 차이가 없었으나, 무첨가구에 비해 패스틴^R 첨가구에서 감소하는 경향을 보였다. 단백질 분해율은 배양 0 시간대에서 4시간대까지는 처리구간 유의성이 없었지만, 12 h과 48 h에서는 패스틴^R 첨가로 시험구에서 감소되었다. 용해 단백질 분해율 “a”는 패스틴^R 시험구에서 경미하게 높은 수치를 나타내었지만, 소화 가능한 단백질 분해율 “a+b”는 패스틴^R 시험구에서 낮은 경향을 보였다. 패스틴^R 첨가로 pH와 NH_3-N 농도는 증가하는 경향이었으며 휘발성지방산, 미생물 수 및 enzyme activity는 감소하였고 영양소 소화율은 높았으나 유의적인 차이는 없었다. This study, including two in vitro experiments and an invivo experiment were conducted to evaluate effects of Passtein^R on crude protein degradability, ruminal fermentation characteristics and nutrient digestibility. In in vitro experiment protein degradability was examined using borate-phosphate buffer and neutral detergent, and using protease from Stroptomyces griseus at 39℃ for 0, 2, 4, 8, 12 and 48 h. In addition, an in vivo experiment was conducted in a switch back design and ruminal fermentation and nutrient digestibility were determined. Four ruminal-fistulated Holstein cows weighing 300㎏ in mean body weight randomly allotted to 2 treatments (control and Passtein^R supplementation). Although there was no significant difference on protein fraction between treatments, it appears that Passtein^R supplementation decreased buffer soluble protein fraction compared to control. Protein degradability was not affected by Passtein^R from 0 h to 4 h, but decreased at 12 h and 48 h compared to control. Degradation of immediately degradable fraction was higher in Passtein^R treatment, but degradation of fermentable fraction was lower in Passtein^R treatment compared to control. The pH and NH_3-N concentration tended to increase in Passtein^R treatment, but VFA production, microbial counts and enzyme activity tended to decrease in Passtein^R treatment compared to control. In addition, nutrient digestibility in the total tract tended to increase in Passtein^R treatment compared to control.

Power and hydrogen production from ammonia in a micro-thermophotovoltaic device integrated with a micro-reformer

Um, D.H.,Kim, T.Y.,Kwon, O.C. Pergamon Press 2014 ENERGY Vol.73 No.-

Power and hydrogen (H<SUB>2</SUB>) production by burning and reforming ammonia (NH<SUB>3</SUB>) in a micro-TPV (microscale-thermophotovoltaic) device integrated with a micro-reformer is studied experimentally. A heat-recirculating micro-emitter with the cyclone and helical adapters that enhance the residence time of fed fuel-air mixtures and uniform burning burns H<SUB>2</SUB>-added NH<SUB>3</SUB>-air mixtures. A micro-reformer that converts NH<SUB>3</SUB> to H<SUB>2</SUB> using ruthenium as a catalyst surrounds the micro-emitter as a heat source. The micro-reformer is surrounded by a chamber, the inner and outer walls of which have installations of gallium antimonide photovoltaic cells and cooling fins. For the micro-reformer-integrated micro-TPV device the maximum overall efficiency of 8.1% with electrical power of 4.5 W and the maximum NH<SUB>3</SUB> conversion rate of 96.0% with the H<SUB>2</SUB> production rate of 22.6 W (based on lower heating value) are obtained, indicating that the overall efficiency is remarkably enhanced compared with 2.0% when the micro-TPV device operates alone. This supports the potential of improving the overall efficiency of a micro-TPV device through integrating it with a micro-reformer. Also, the feasibility of using NH<SUB>3</SUB> as a carbon-free fuel for both burning and reforming in practical micro power and H<SUB>2</SUB> generation devices has been demonstrated.

Pentoxifvlline을 이용한 정자 처리가 체외수정시술에 미치는 영향에 관한 연구

문신용,김정훈,엄정윤,노미경,오선경,김석현,최영민,신창재,김정구,이진용,장윤석 대한산부인과내시경학회 1994 대한산부인과내시경학회잡지 Vol.6 No.1

Engineering of Corynebacterium glutamicum to utilize methyl acetate, a potential feedstock derived by carbonylation of methanol with CO

Choo, S.,Um, Y.,Han, S.O.,Woo, H.M. Elsevier Science Publishers 2016 Journal of biotechnology Vol.224 No.-

<P>The possibilities to utilize one-carbon substrates (C-1) like CO, methane and methanol have been explored as a cheap alternative feedstock in the biotechnology. For the first time, methyl acetate (MeOAc), which can be formed from carbonylation of methanol with CO, was demonstrated to be an alternative carbon source for the cell growth of Corynebacterium glutamicum as a model microbial cell factory. To do so, a carboxyl esterase activity was necessary to hydrolyze MeOAc to methanol and acetate. Although the wild-type has an unknown esterase activity to MeOAc, the activity was not high enough to grow from 270 mM MeOAc as sole carbon source, reaching OD600 of 5.28 +/- 0.2 in 32 h. Based on the literatures studied for the esterase, we chose three esterases (MekB of Pseudomonas veronii MEK700, AcmB of Gordonia sp. Strain TY-5, and Est of Pyrobaculum calidifontis VA1) and cloned into the wild-type. As a result, the recombinant C. glutamicum expressing the highly active MekB esterase (28.6 +/- 0.77 U/mg protein) showed complete degradation of MeOAc and utilization of acetate, resulting in OD600 of 16.5 +/- 0.02 at 24 h. In addition, the recombinant strain exhibited the rapid degradation of MeOAc to methanol and acetate in 2 h under anaerobic condition. Therefore, MeOAc can be used as another C-1-derived carbon source in the biotechnology. (C) 2016 Elsevier B.V. All rights reserved.</P>

ASXL2 promotes proliferation of breast cancer cells by linking ERα to histone methylation

Park, U-H,Kang, M-R,Kim, E-J,Kwon, Y-S,Hur, W,Yoon, S K,Song, B-J,Park, J H,Hwang, J-T,Jeong, J-C,Um, S-J Macmillan Publishers Limited 2016 Oncogene Vol.35 No.28

<P>Estrogen receptor alpha (ER alpha) has a pivotal role in breast carcinogenesis by associating with various cellular factors. Selective expression of additional sex comb-like 2 (ASXL2) in ER alpha-positive breast cancer cells prompted us to investigate its role in chromatin modification required for ERa activation and breast carcinogenesis. Here, we observed that ASXL2 interacts with ligand E2-bound ERa and mediates ERa activation. Chromatin immunoprecipitation-sequencing analysis supports a positive role of ASXL2 at ERa target gene promoters. ASXL2 forms a complex with histone methylation modifiers including LSD1, UTX and MLL2, which all are recruited to the E2-responsive genes via ASXL2 and regulate methylations at histone H3 lysine 4, 9 and 27. The preferential binding of the PHD finger of ASXL2 to the dimethylated H3 lysine 4 may account for its requirement for ERa activation. On ASXL2 depletion, the proliferative potential of MCF7 cells and tumor size of xenograft mice decreased. Together with our finding on the higher ASXL2 expression in ERa-positive patients, we propose that ASXL2 could be a novel prognostic marker in breast cancer.</P>

Photoelectrochemical water splitting employing a tapered silicon nanohole array

Jung, J. Y.,Choi, M.,Zhou, K.,Li, X.,Jee, S. W.,Um, H. D.,Park, M. J.,Park, K. T.,Bang, J.,Lee, J. H. Royal Society of Chemistry 2014 Journal of Materials Chemistry A Vol.2 No.3

An effective photocathode adopting a tapered Si nanohole (SiNH) array has been developed for photoelectrochemical water splitting. The tapered feature of SiNH photocathodes resulted in a gradation of the refractive indices between Si and air, such that the mismatching of optical impedance was alleviated and light absorption was enhanced. Adjusting the depth of the SiNHs successfully simulated the number of dielectric layers, optimizing the destructive interference for an antireflective coating (ARC). Only a 200 nm-thin NH array was required to absorb similar to 96% of solar spectral irradiance for photoelectrochemical applications. This thickness also minimized the undesirable surface recombination loss. When compared to a similar system using a planar technology, the formation of NHs was observed to cause an increase in the optical bandgap. This could generate a surface-passivation effect, resulting in a lowering of dark current and an increase in photovoltage, which could be utilized for an anodic shift of the onset voltage. Due to the addition of tapered SiNHs, the photogenerated current was improved by similar to 30% (similar to 33 mA cm(-2)) compared to a planar counterpart (similar to 25 mA cm(-2)), while the overpotential required for H-2 evolution was reduced.

향신료가 Salivary Amylase에 미치는 영향

김혜영,엄대자,한혜기 이화여자대학교 의과대학 1975 梨花醫學誌 Vol.- No.7

The effects of the various concentrations of condiments upon amylase activity, condiments such as sodium glutamic acid, sugar, salt, welsh onion, garlic, ginger, re pepper, black pepper and mustard were determined. And the result was obtained as follows; 1. Amylase activity was increased by glutamic acid and was activated by salt and sugar a little. 2. Amylase activity was inhibited by welsh onion, ginger, garlic, red pepper and mustard, especially red pepper was strong.

고온기 유리온실의 냉방방법별 냉방효과 구명

최영하,엄영철 韓國施設園藝硏究會 1999 施設園藝硏究 Vol.12 No.1

향신료가 Salivary Amylase에 미치는 영향

김혜영,엄대자,한혜기 梨花女子大學校 醫科大學 1974 梨花醫學誌 Vol.- No.7