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Numerical simulation and experiments of magnetic flux leakage inspection in pipeline steel
Xun-Bo Li,Xiang Li,Liang Chen,Pei-Fu Feng,Hai-Dong Wang,Zuo-Ying Huang 대한기계학회 2009 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.23 No.1
The magnetic flux leakage (MFL) method is currently the most commonly used pipeline inspection technique. In this paper, numerical simulation and experimental investigation on defect inspection in pipeline steel using MFL were carried out. In theoretical analysis, typical three-dimensional (3D) defects were accurately modeled and detailed MFL signals in the test surface were calculated by 3D finite element method (FEM). To confirm the 3D FEM results, different artificial defects were made and the MFL experiments were performed. The experimental study demonstrated that the results were agreement with the 3D FEM result. The results show that the 3D FEM is an effective analysis method for pipeline steel MFL inspection.
Liang, Shuang,Guo, Jing,Jin, Yong Xun,Yuan, Bao,Zhang, Jia-Bao,Kim, Nam-Hyung Elsevier 2018 Theriogenology Vol.106 No.-
<P><B>Abstract</B></P> <P>C-Phycocyanin (C-PC), a protein from green microalgae, has been suggested to possess various biological activities, including antioxidant and free radical scavenging properties. The aim of the current study was to explore the effects of C-PC on the maturation of porcine oocytes and subsequent developmental competence after parthenogenetic activation and somatic cell nuclear transfer (SCNT) as well as the underlying mechanisms. There was no significant improvement in nuclear maturation rates between the control and C-PC supplementation groups (1, 3, 5, 10 μg/mL). However, supplementation of 5 μg/mL C-PC in the maturation medium significantly increased blastocyst formation and hatching rates after parthenogenetic activation (59.6 ± 3.6% and 33.0 ± 2.6% vs. 49.8 ± 3.5% and 27.4 ± 2.4%, respectively). In addition, the presence of C-PC during the maturation period significantly improved blastocyst formation rates and total cell numbers after SCNT (24.8 ± 1.9% and 42.2 ± 3.3 vs. 21.6 ± 2.2% and 39.5 ± 3.4, respectively) compared to the control group. Furthermore, cellular proliferation and the expression of pluripotency-related genes (<I>SOX2</I> and <I>NANOG</I>) were increased in cloned blastocysts derived from the C-PC supplemented group. Importantly, C-PC supplementation during maturation not only improved cumulus expansion and increased the expression of cumulus expansion-related genes (<I>HAS2</I>, <I>PTX3</I>, and <I>PTGS2</I>), but also enhanced antioxidant capacity, improved mitochondria function, and decreased cathepsin B activity in porcine oocytes. These results demonstrate that C-PC may be useful for improving porcine oocyte quality and subsequent developmental competence in embryos.</P> <P><B>Highlights</B></P> <P> <UL> <LI> C-Phycocyanin significantly increased the developmental competence of porcine parthenogenetic and cloned embryos. </LI> <LI> C-Phycocyanin enhanced antioxidant capacity, improved mitochondria function, and decreased cathepsin B activity in porcine oocytes. </LI> </UL> </P>
Liang, Shuang,Yuan, Bao,Jin, Yong-Xun,Zhang, Jia-Bao,Bang, Jeong Kyu,Kim, Nam-Hyung CSIRO Publishing 2017 Reproduction, fertility, and development Vol.29 No.11
<P> Cryopreservation is an effective method for the long-term storage of valuable germplasm in the field of reproductive research. The present study examined the developmental capacity of post-thaw bovine blastocysts during vitrification after supplementation with antifreeze glycoprotein 8 (AFGP8). Survival and re-expansion rates in culture during the 12 h after thawing were significantly higher in the AFGP8-treated than untreated group. In addition, blastocysts from the AFGP8-treated group exhibited lower rates of apoptosis. Real-time reverse transcription-polymerase chain reaction analysis showed that the expression of the Bcl-2 gene, coding for an anti-apoptotic protein, was increased significantly, whereas the expression of the pro-apoptotic gene Bax was decreased significantly in the AFGP8-treated group. The cellular proliferation rate and mitochondrial membrane potential were significantly higher in post-thaw re-expanded blastocysts from the AFGP8-treated compared with untreated group. In addition, outgrowth potential in post-thaw blastocysts in re-expanded blastocysts after vitrification was significantly increased in the AFGP8-treated compared with untreated group. Together, these results are the first to demonstrate that the addition of AFGP8 during vitrification can help protect bovine blastocysts against chill-induced injury. </P>
TSA Inhibit in Prolonging GVBD which Results Artificial Control of Oocytes Maturation Time
Yong-Xun Jin,Xing-Wei Liang,Sung-Hyun Lee,Xiang-Shun Cui,Nam-Hyung Kim 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s
Although evidences showed that histone deacetylation plays an important role in the mitotic and meiotic cell cycle, but the mechanisms are still unclear. Level of histone acetylation can be easily changed by deacetylase inhibitors (HDACi) i.e trichostatin A (TSA) and valporic acid. In this study, we determined whether the inhibition of histone deacetylation by TSA could affect porcine oocyte maturation and aging process. Our results showed that treated COCs with 100 nM TSA significantly increase the GVBD in each time group than 0, 5, 50 nM but no significantly different from that of higher concentration (200 nm or 300 nM). No significant differences on maturation, blastocyst development, MAPK pattern and expressions of apoptosis gene when treated oocytes with 100 nM TSA for the first 24h of IVM compared with control and 5, 50 nM TSA. However, in the oocytes treated with 200 nM and 300 nM TSA for first 24 h, MAPK significantly decreased and abnormal spindle were observed. But, in prolonged (64 h) of TSA treated group has no significantly different in control. Another data observed that after 24h TSA-treat to prolonged group were significantly decreased of MAPK activation and normal spindle than the other group. We concluded that TSA played a critical role in meiotic progression in porcine oocytes through the regulation of arrest GVBD, which prolonging the in vitro maturation time, but unaffected the subsequent pre-implantation embryo developmental potential and embryonic qualities. Moreover, the histone deacetylase inhibitor TSA may artificially control porcine oocyte maturation time and delay porcine oocyte aging process.