Effects of dietary supplementation of herbal active ingredients promoting insulin-like growth factor-1 secretion on production performance, egg quality, blood hematology, and excreta gas emission in laying hens

Dang, De Xin,Chung, Yi Hyung,Kim, In Ho Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.11

Objective: The purpose of this study was to evaluate the effects of supplementing herbal active ingredients (YGF251) which can promote the secretion of insulin-like growth factor-1 (IGF-1) in the diet on production performance, egg quality, blood hematology, and excreta gas emission in laying hens. Methods: A total of 288 ISA Brown (41-week-old) laying hens with an initial body weight of 1.83±0.68 kg were randomly assigned to 1 of 4 dietary treatments in a randomized block design based on body weight. Each treatment had 12 replicate cages having 6 adjacent cages per replicate (hens are kept in cages alone). The experimental period was 35 days. Dietary treatments were based on the corn-soybean meal-wheat-based basal diet and supplemented with 0.00%, 0.05%, 0.10%, or 0.15% YGF251. Results: There was a linear increased egg weight in weeks 1 to 5 (p<0.05), egg mass in week 1 (p<0.05) and weeks 1 to 5 (p<0.05), egg strength on day 7 (p<0.05), 21 (p<0.01), and 35 (p<0.01), eggshell thickness on day 21 (p<0.05) and 35 (p<0.01), haugh unit on day 21 (p<0.01) and 35 (p<0.05), serum IGF-1 concentration on day 21 (p<0.05) and 35 (p<0.01), and serum total protein concentration on day 35 (p<0.05) were observed with the supplementing YGF251 increased in the diet, while feed conversion ratio in weeks 1 to 5 (p<0.05) and excreta ammonia emission (p<0.01) decreased linearly with the dose of YGF251 increased. Conclusion: Dietary supplementation of YGF251 positively affected the production performance and egg quality of laying hens through increasing serum IGF-1 concentration in a dose-dependent manner. Moreover, YGF251 supplementation improved barn environment by reducing excreta noxious gas emission.

Silybum marianum seed extract supplementation positively affects the body weight of weaned piglets by improving voluntary feed intake

De Xin Dang,Sung Bo Cho,In Ho Kim 한국축산학회 2022 한국축산학회지 Vol.64 No.4

This study was conducted to evaluate the effects of dietary supplementation of Silybum marianum seed (SMS) extract on the growth performance, nutrient digestibility, fecal noxious gas emission, and hematology parameters in weaned piglets. A total of 120, 21-day-old weaned piglets ([Yorkshire × Landrace] × Duroc) were randomly assigned to 3 groups based on the average initial body weight (6.46 ± 0.45 kg). There were 8 replicate pens per treatment and 5 pigs (mixed sex) per pen. The experimental period was 42 days. Dietary groups included a basal diet, and a basal diet supplemented with 0.05% or 0.10% SMS extract. Feeding weaned piglets with SMS extract containing diet significantly increased average daily gain and average daily feed intake. Additionally, the supplementation of SMS extract had no significant effects on nutrient digestibility, serum hematology, and fecal noxious gas emission parameters. We considered that the supplementation of SMS extract had positive effects on the voluntary feed intake in weaned piglets, thus improving growth performance.

Design and Research on the LQR Controller for the Magnet Power Supply of the Accelerator based on Particle Swarm Optimization

Xin Zhang,Jianwu Dang,Yangping Wang 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.9

Accelerator has been widely applied to high energy, low energy physics, Medical environmental and military fields, etc. The magnet power supply is one of the most important parts of the accelerator. While the magnet power supply of accelerator is taken as the control object, and the design of LQR controller of the magnet power supply based on PSO has been proposed. At the beginning, the state-space model of the magnet power supply has been established by modern control theories. Then, the PSO (Particle Swarm Optimization) algorithm has been used for the weight matrices optimization of LQR controller in order to ensure that the magnet power supply can provide a magnetic field quickly which the accelerator required. The experimental results indicate that the method that proposed in the paper can meet the requirements of fast output response of the system and each control index of the LQR controller is obviously superior to that by the traditional method.

Controlling charge injection properties in polymer field-effect transistors by incorporation of solution processed molybdenum trioxide

Long, Dang Xuan,Xu, Yong,Wei, Huai-xin,Liu, Chuan,Noh, Yong-Young The Royal Society of Chemistry 2015 Physical chemistry chemical physics Vol.17 No.31

<P>A simply and facilely synthesized MoO<SUB>3</SUB> solution was developed to fabricate charge injection layers for improving the charge-injection properties in p-type organic field-effect transistors (OFETs). By dissolving MoO<SUB>3</SUB> powder in ammonium (NH<SUB>3</SUB>) solvent under an air atmosphere, an intermediate ammonium molybdate ((NH<SUB>4</SUB>)<SUB>2</SUB>MoO<SUB>4</SUB>) precursor is made stable, transparent and spin-coated to form the MoO<SUB>3</SUB> interfacial layers, the thickness and morphology of which can be well-controlled. When the MoO<SUB>3</SUB> layer was applied to OFETs with a cost-effective molybdenum (Mo) electrode, the field-effect mobility (<I>μ</I><SUB>FET</SUB>) was significantly improved to 0.17 or 1.85 cm<SUP>2</SUP> V<SUP>−1</SUP>s<SUP>−1</SUP> for polymer semiconductors, regioregular poly(3-hexylthiophene) (P3HT) or 3,6-bis-(5bromo-thiophen-2-yl)-<I>N</I>,<I>N</I>′-bis(2-octyl-1-dodecyl)-1,4-dioxo-pyrrolo[3,4-<I>c</I>]pyrrole (DPPT-TT), respectively. Device analysis indicates that the MoO<SUB>3</SUB>-deposited Mo contact exhibits a contact resistance <I>R</I><SUB>C</SUB> of 1.2 MΩ cm comparable to that in a device with the noble Au electrode. Kelvin-probe measurements show that the work function of the Mo electrode did not exhibit a dependence on the thickness of MoO<SUB>3</SUB> film. Instead, ultraviolet photoemission spectroscopy results show that a doping effect is probably induced by casting the MoO<SUB>3</SUB> layer on the P3HT semiconductor, which leads to the improved hole injection.</P> <P>Graphic Abstract</P><P>A simply synthesized MoO<SUB>3</SUB> is used as charge injection layers for printed p-type organic field-effect transistors (OFETs). <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5cp03369a'> </P>

Assessment of Neoadjuvant Treatment Response Using Automated Breast Ultrasound in Breast Cancer

Xiaozhi Dang,Xin Zhang,Yi Gao,Hongping Song 한국유방암학회 2022 Journal of breast cancer Vol.25 No.4

Breast imaging techniques are used to assess the tumor response to neoadjuvant treatment (NAT), which is increasingly one of the preferred therapeutic options and increases the rate of breast conservation for breast cancer. Herein, we report a case in which a woman was diagnosed with invasive ductal carcinoma in the left breast and received NAT before surgery. Automated breast ultrasound (AB US) was regularly performed before and during the NAT to evaluate the tumor response to NAT by measuring diameter changes and volume reductions of the tumor. Images showed that the tumor size was significantly reduced and disappeared after 7 cycles of NAT, except for macrocalcification. Postoperative histopathological examination confirmed that there were no residual tumor cells. We found that AB US overcame the limitations of handheld US, such as operator dependence, poor reproducibility and limited field of view, and can be an alternative modality to assess the tumor response of NAT in the absence of magnetic resonance imaging (MRI) instruments.

Antimicrobial susceptibility and molecular detection of chloramphenicol and florfenicol resistance among Escherichia coli isolates from diseased chickens

Xiang-Dang Du,Xin-Sheng Li,Gui-Qin Wang,Bao-An Cui,Su-Mei Zhang,Jian-Zhong Shen 대한수의학회 2007 JOURNAL OF VETERINARY SCIENCE Vol.8 No.3

Seventy Escherichia coli isolates recovered from diseased chickens diagnosed with colibacillosis in Henan Province, China, between 2004 and 2005 were characterized for antimicrobial susceptibility profiles via a broth doubling dilution method. Overall, the isolates displayed resistance to trimethoprim-sulfamethoxazole (100%), oxytetracycline (100%), ampicillin (83%), enrofloxacin (83%), and ciprofloxacin (81%), respectively. Among the phenicols, resistance was approximately 79% and 29% for chloramphenicol and florfenicol, respectively. Molecular detection revealed that the incidence rates of the floR, cmlA, cat1, cat2 and cat3 were 29, 31, 16, 13, and 0%, respectively. Additionally, 10% of the isolates were positive for both floR and cmlA. As these antimicrobial agents may potentially induce cross-resistance between animal and human bacterial pathogens, their prudent use in veterinary medicine is highly recommended.

miR-98 suppresses melanoma metastasis through a negative feedback loop with its target gene IL-6

Fei Li,Xin-ji Li,Li Qiao,Fei Shi,Wen Liu,You Li,Yu-ping Dang,Weijie Gu,Xiao-gang Wang,Wei Liu 생화학분자생물학회 2014 Experimental and molecular medicine Vol.46 No.-

Dysregulated microRNA (miRNA) expression has a critical role in tumor development and metastasis. However, the mechanism by which miRNAs control melanoma metastasis is unknown. Here, we report reduced miR-98 expression in melanoma tissues with increasing tumor stage as well as metastasis; its expression is also negatively associated with melanoma patient survival. Furthermore, we demonstrate that miR-98 inhibits melanoma cell migration in vitro as well as metastatic tumor size in vivo. We also found that IL-6 is a target gene of miR-98, and IL-6 represses miR-98 levels via the Stat3-NF-κB-lin28B pathway. In an in vivo melanoma model, we demonstrate that miR-98 reduces melanoma metastasis and increases survival in part by reducing IL-6 levels; it also decreases Stat3 and p65 phosphorylation as well as lin28B mRNA levels. These results suggest that miR-98 inhibits melanoma metastasis in part through a novel miR-98-IL-6-negative feedback loop.

Deubiquitinating enzyme Josephin-2 stabilizes PHGDH to promote a cancer stem cell phenotype in hepatocellular carcinoma

Wang Ying,Li Ze-Xin,Wang Jian-Guo,Li Lu-Hao,Shen Wen-Long,Dang Xiao-Wei 한국유전학회 2023 Genes & Genomics Vol.45 No.2

Background Deubiquitinating enzymes (DUBs) have been shown to be possible targets for hepatocellular carcinoma (HCC) treatment. Objective This study was designed to reveal the effect and underlying mechanism of Josephin-2, a relatively newly defined DUB, in HCC progression. Methods SNU-387 and PLC/PRF/5 cells were used for in vitro functional assays. The levels of Josephin-2 and phosphoglycerate dehydrogenase (PHGDH) were determined using RT-qPCR and western blotting. Cell proliferation, migration and invasion were assessed by CCK-8, colony formation and Transwell. Spheroid-forming assay was performed to assess the cancer stem cell (CSC)-phenotype of HCC cells. A xenograft mice model was applied to verify the effect of Josephin-2 on HCC cell growth in vivo. Results Herein, we showed that Josephin-2 expression was negatively correlated with HCC patient survival in data from the online database. Cell experiments indicated that knockdown of Josephin-2 attenuated HCC cell malignant biological behaviors. Besides, Josephin-2 silencing also decreased the spheroid-formation while inhibited the expression of CSC biomarkers (CD133, OCT4, SOX2 and EpCAM) in HCC cells. Mechanistically, Josephin-2 had a deubiquitinating activity towards the regulation of PHGDH protein, the rate-limiting enzyme in the first step of serine biosynthesis pathway. Depletion of Josephin-2 enhanced the ubiquitination degradation of PHGDH and ultimately inhibited the proliferation and CSC-phenotype of HCC in vitro and in vivo. Conclusion Our work uncovered the regulatory effects of Josephin-2 on PHGDH protein stability and profiled its contribution in HCC malignant progression, which might provide a potential therapeutic target for HCC.

Effect of Sophora flavescens on the pharmacokinetics of carbamazepine in rats

Lei Shi,Xue-Liang Dang,Xin-You Liu,Hua-Mei Wei,Meng-Meng Yang,Yan Zhang 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.12

Carbamazepine (CBZ), an antiepilepticwith narrow therapeutic window, is a substrate ofCYP 3A4 which metabolizes CBZ to carbamazepine-10,11-epoxide (CBZE). CBZE is an active and toxicitymetabolite, and it is a substrate of MRP-2. UsingCBZ for a long time can cause hepatic injury. Sophora flavescens (SF) is a medicinal herb used forthe protected hepatic injury. This study investigatedthe acute and chronic effects of SF on the pharmacokineticsof CBZ in rats. The concentrations of CBZand CBZE in plasma and tissues were determined byHPLC method. The results showed that SF whichsignificantly decreased the AUC0-t of CBZ, increasedCBZE conversely. Tissue analysis showed that theconcentrations of CBZ and CBZE in brain and liverwere decreased by SF. In addition, the distribution ofCBZE in kidney was reduced significantly, whichinfluenced the CBZE excretion and increased the drugtoxic potentially. Results in the current study suggestthat patients using CBZ might be cautioned in the useof SF extract or Sophora-derived products. Meanwhile,patients receiving drugs which are substrates ofCYP 3A4 and/or MRP-2 should be advised of thepotential herb–drug interaction to reduce the risk oftherapeutic failure or increased toxicity of conventionaldrug therapy.

Comparison of the effects of zinc oxide and zinc aspartic acid chelate on the performance of weaning pigs

Sarbani Biswas,De Xin Dang,In Ho Kim 한국축산학회 2024 한국축산학회지 Vol.66 No.1

In this research, the growth efficiency, nutritional utilization, fecal microbial levels, and fecal score of weaned pigs were evaluated using therapeutic zinc oxide (ZnO) and zinc aspartic acid chelate (Zn-Asp). In a 42-day feeding trial, 60 weaned pigs ([Yorkshire × Landrace] × Duroc) were arbitrarily allotted (age: 21 days; 7.01 ± 0.65 kg preliminary body weight) to 3 different treatment groups with 5 repetitions (2 male and 2 female piglets) in each pen. The trial had 2 different phases, including 1–21 days as phase 1, and 22–42 days as phase 2. The nutritional treatments were: basal diet as control (CON), basal diet incorporated with 3,000 ppm ZnO as TRT1, and basal diet incorporated with 750 ppm Zn-Asp as TRT2. In comparison to the CON group, the pigs in the TRT1 and TRT2 groups had greater (p < 0.05) body weight on day 42; an average daily gain, and an average daily feed intake on days 22–42. Furthermore, during days 1-42, the average daily gain in the treatment groups trended higher (p < 0.05) than in the CON group. Additionally, the fecal score decreased (p < 0.05) at week 6, the lactic acid bacteria count tended to increase (p < 0.05), and coliform bacteria presented a trend in reduction (p < 0.05) in the TRT1 and TRT2 groups compared to the CON group. However, there was no difference in nutrient utilization (p > 0.05) among the dietary treatments. Briefly, the therapeutic ZnO and Zn-Asp nutritional approaches could decrease fecal score and coliform bacteria, increase lactic acid bacteria, and improve growth efficiency; moreover, Zn- Asp (750 ppm) can perform a comparable role to therapeutic ZnO (3,000 ppm). So we can use Zn-Asp (750 ppm) instead of therapeutic ZnO (3,000 ppm) for the better performance of weaning pigs and the reduction of environmental pollution, as therapeutic ZnO is responsible for environmental pollution.