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        Photocatalytic removal of cefazolin using Ag3PO4/BiOBr under visible light and optimization of parameters by response surface methodology

        Yuhui Xiao,Xianghua Song,Zhuo Liu,Ruiping Li,Xiaorong Zhao,Yingping Huang 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.45 No.-

        The degradation of cefazolin (CFZ) by Ag3PO4/BiOBr composites under visible-light irradiation wasexplored. The main and interaction of parameters (catalyst dosage, pH, CFZ initial concentration anddegradation time) on removal of CFZ were studied by Box–Behnken design combined with responsesurface methodology. The pH was the most influential factor and both h+ and OH played a role in thephotocatalytic process. The high correlation coefficients (R2 = 0.9986 and adjusted R2 = 0.9973)demonstrated closefit between the predicted and experimental values. The exceptional efficiency ofAg3PO4/BiOBr composite in removing CFZ represents a promising technique for treatment of CFZcontainingwastewater.

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        OsBAK1 is involved in rice resistance to Xanthomonas oryzae pv. oryzae PXO99

        Hualan Liao,Xiaorong Xiao,Xiuqiong Li,Yan Chen,Xiumei Fu,Daozhe Lin,Xiaolei Niu,Yinhua Chen,Chaozu He 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.2

        OsBAK1 gene belongs to a receptor like kinase gene family in rice and shares a highly conserved gene structure and sequence homology with Arabidopsis thaliana BAK1 gene. To investigate the role of OsBAK1 in rice immunity, the full-length cDNA of OsBAK1 was isolated by RT-PCR and the transgenic rice lines (over expression and RNA-interference lines) were generated using Agrobacterium-mediated transformation. The expression level of OsBAK1 was determined by q-PCR in overexpression and RNAi transgenic rice lines. Based on quantitative polymerase chain reaction (q-PCR) results, two overexpression lines and two RNAi lines were evaluated in bioassays for resistance to Xanthomonas oryzae pv. oryzae PXO99, the causal agent of rice bacterial blight disease. Pathogenicity bioassays showed overexpression OsBAK1 lines exhibited resistance to blight disease whereas OsBAK1 RNAi lines promoted susceptibility. Besides, OsBAK1 can complement the function of AtBAK1 in Arabidopsis bak1 protoplast, activating FRK1 expression, a marker gene in PTI signaling pathway, after treatment by flg22. Furthermore, the transcriptional level of OsBAK1 was induced significantly in rice by defense signaling molecules such as salicylic acid, jasmonic acid, and PXO99 inoculation. Our results illustrated OsBAK1 positively regulates plant defense against rice bacterium pathogen Xanthomonas oryzae pv. oryzae PXO99.

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