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Solidification of uranium mill tailings by MBS-MICP and environmental implications
Niu Qianjin,Li Chunguang,Liu Zhenzhong,Li Yongmei,Meng Shuo,He Xinqi,Liu Xinfeng,Wang Wenji,He Meijiao,Yang Xiaolei,Liu Qi,Liu Longcheng 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.10
Uranium mill tailing ponds (UMTPs) are risk source of debris flow and a critical source of environmental U and Rn pollution. The technology of microbial induced calcium carbonate precipitation (MICP) has been extensively studied on reinforcement of UMTs, while little attention has been paid to the effects of MICP on U & Rn release, especially when incorporation of metakaolin and bacillus subtilis (MBS). In this study, the reinforcement and U & Rn immobilization role of MBS -MICP solidification in different grouting cycle for uranium mill tailings (UMTs) was comprehensively investigated. The results showed that under the action of about 166.7 g/L metakaolin and ~50% bacillus subtilis, the solidification cycle of MICP was shortened by 50%, the solidified bodies became brittle, and the axial stress increased by up to 7.9%, and U immobilization rates and Rn exhalation rates decrease by 12.6% and 0.8%, respectively. Therefore, the incorporation of MBS can enhance the triaxial compressive strength and improve the immobilization capacity of U and Rn of the UMTs bodies solidified during MICP, due to the reduction of pore volume and surface area, the formation of more crystals general gypsum and gismondine, as well as the enhancing of coprecipitation and encapsulation capacity
Preparation and evaluation of porous H1.6Mn1.6O4@chitosan pellet for Li+ extraction
Xiaoxian Zhang,Yue Niu,Feng Xue,Jianhong Gao,Xiaolei Zhu,Shengui Ju 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.10
Spinel-structured lithium manganese oxide is regarded as one of the most promising materials that can recover Li+ from brine and seawater. Herein, a hierarchical porous and hydrophilic H1.6Mn1.6O4@chitosan pellet (HMO@CP) is proposed and its mechanical property is tailored through the glutaraldehyde-derived cross-linking. Different characterization techniques such as scanning electron microscopy (SEM), Brunner-Emmet-Teller (BET) measurement, Fourier transformation infrared spectrum (FTIR), and X-ray diffraction (XRD) meter were used to investigate the chemical and morphological properties of the HMO@CP. H1.6Mn1.6O4 powders were successfully encapsulated by chitosan, forming composite porous pellets. The equilibrium adsorption capacity of HMO@CP is 49.2mg·g1, which is similar to that of the pristine H1.6Mn1.6O4. Moreover, the adsorption behavior of HMO@CP well fits with the pseudosecond- order kinetic model, and the Langmuir model can be used to describe the adsorption isotherm of HMO@CP. Furthermore, the adsorption thermodynamic parameters such as H, G and S were calculated based on the obtained results. When the pellet is immersed into 0.05mol·L1 HCl solution after the Li+ adsorption process, the desorption equilibrium can be reached within 60 min, with a manganese dissolution loss of 2.48%. The Li+ adsorption capacity of HMO@CP remains at 41.92mg·g1 after five adsorption-desorption cycles, confirming the effective regeneration property of the HMO@CP. In addition, the as-prepared HMO@CP shows excellent selectivity for Li+ among Na+, K+, Mg2+, and Ca2+ ions in the simulated solution.
OsBAK1 is involved in rice resistance to Xanthomonas oryzae pv. oryzae PXO99
Hualan Liao,Xiaorong Xiao,Xiuqiong Li,Yan Chen,Xiumei Fu,Daozhe Lin,Xiaolei Niu,Yinhua Chen,Chaozu He 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.2
OsBAK1 gene belongs to a receptor like kinase gene family in rice and shares a highly conserved gene structure and sequence homology with Arabidopsis thaliana BAK1 gene. To investigate the role of OsBAK1 in rice immunity, the full-length cDNA of OsBAK1 was isolated by RT-PCR and the transgenic rice lines (over expression and RNA-interference lines) were generated using Agrobacterium-mediated transformation. The expression level of OsBAK1 was determined by q-PCR in overexpression and RNAi transgenic rice lines. Based on quantitative polymerase chain reaction (q-PCR) results, two overexpression lines and two RNAi lines were evaluated in bioassays for resistance to Xanthomonas oryzae pv. oryzae PXO99, the causal agent of rice bacterial blight disease. Pathogenicity bioassays showed overexpression OsBAK1 lines exhibited resistance to blight disease whereas OsBAK1 RNAi lines promoted susceptibility. Besides, OsBAK1 can complement the function of AtBAK1 in Arabidopsis bak1 protoplast, activating FRK1 expression, a marker gene in PTI signaling pathway, after treatment by flg22. Furthermore, the transcriptional level of OsBAK1 was induced significantly in rice by defense signaling molecules such as salicylic acid, jasmonic acid, and PXO99 inoculation. Our results illustrated OsBAK1 positively regulates plant defense against rice bacterium pathogen Xanthomonas oryzae pv. oryzae PXO99.