Preparation and properties of Nd-doped BCTH lead-free ceramics by solid-phase twin crystal method

He Xiyue,Fang Bijun,Zhang Shuai,Lu Xiaolong,Ding Jianning 한국물리학회 2022 Current Applied Physics Vol.38 No.-

0.5 mol% Nd-doped (Ba0.85Ca0.15)(Ti0.9Hf0.1)O3 (BCTH-Nd) lead-free ceramics were prepared by a solid-phase twin crystal method, where the effects of sintering condition on structure, electrical and optical properties were studied. All the sintered BCTH-Nd ceramics exhibit pure perovskite structure, dense microstructure with several micron grain size, which tends to increase with elevating sintering temperature. All synthesized ceramics have complex dielectric behavior, which presents normal ferroelectrics characteristic with slight dispersion phenomenon. The BCTH-Nd ceramics exhibit excellent piezoelectric and ferroelectric properties and acceptable dielectric performance when sintered at 1480 ◦C for 2 h. Under 269 nm light excitation, several fluorescent emission peaks are excited with a whole indigo fluorescence, where the strongest emission peak is emitted at 473 nm, corresponding to the 4G3/2 → 4I9/2 energy level transition of Nd3+. Multifunctional performance is fulfilled in the lead-free BCTH ceramics via rare earth doping, which can broaden the application fields of piezoelectricbased materials.

Weak fault detection for wind turbine bearing based on ACYCBD and IESB

Xiaolong Wang,Xiaoli Yan,Yuling He 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.4

To detect the rolling bearing fault of a wind turbine at incipient injury phase, a novel method combining adaptive cyclostationary blind deconvolution (ACYCBD) with instantaneous energy slice bispectrum (IESB) is proposed. Cuckoo search algorithm (CSA) is fused with cyclostationary blind deconvolution (CYCBD) to optimize cyclic frequency and filter length parameters, and the satisfied processing result is able to adaptively use this developed ACYCBD method. Besides, fusing the complementary superiorities of frequency weighted energy operator and slice bispectrum, a new frequency domain analysis method named IESB is put forward to identify the characteristic frequency components. During fault diagnosis, the deconvolution signal with higher signal to noise ratio is first separated from the collected signal by applying ACYCBD method. However, owing to the intense background noises, external interferences still remain in the deconvolution signal. Then the IESB is further used as a post-processing method, the residual noises are suppressed effectively, and the characteristic spectral lines are highlighted immensely. In the end, the incipient fault injury of wind turbine bearing can be diagnosed by analyzing the acquired instantaneous energy slice bispectrum. The analyzed results of wind turbine bearing fault signals from the experimental rig and the actual engineering testing field demonstrate the feasibility and the superiority of this novel detection method.

Genome wide association study on feed conversion ratio using imputed sequence data in chickens

Jiaying Wang,Xiaolong Yuan,Shaopan Ye,Shuwen Huang,Yingting He,Hao Zhang,Jiaqi Li,Xiquan Zhang,Zhe Zhang 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.4

Objective: Feed consumption contributes a large percentage for total production costs in the poultry industry. Detecting genes associated with feeding traits will be of benefit to improve our understanding of the molecular determinants for feed efficiency. The objective of this study was to identify candidate genes associated with feed conversion ratio (FCR) via genome-wide association study (GWAS) using sequence data imputed from single nucleotide polymorphism (SNP) panel in a Chinese indigenous chicken population. Methods: A total of 435 Chinese indigenous chickens were phenotyped for FCR and were genotyped using a 600K SNP genotyping array. Twenty-four birds were selected for sequencing, and the 600K SNP panel data were imputed to whole sequence data with the 24 birds as the reference. The GWAS were performed with GEMMA software. Results: After quality control, 8,626,020 SNPs were used for sequence based GWAS, in which ten significant genomic regions were detected to be associated with FCR. Ten candidate genes, ubiquitin specific peptidase 44, leukotriene A4 hydrolase, ETS transcription factor, R-spondin 2, inhibitor of apoptosis protein 3, sosondowah ankyrin repeat domain family member D, calmodulin regulated spectrin associated protein family member 2, zinc finger and BTB domain containing 41, potassium sodium-activated channel subfamily T member 2, and member of RAS oncogene family were annotated. Several of them were within or near the reported FCR quantitative trait loci, and others were newly reported. Conclusion: Results from this study provide valuable prior information on chicken genomic breeding programs, and potentially improve our understanding of the molecular mechanism for feeding traits.

Diagnosis of compound faults of rolling bearings through adaptive maximum correlated kurtosis deconvolution

Guiji Tang,Xiaolong Wang,Yuling He 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.1

This paper proposes a new diagnosis method based on Adaptive maximum correlated kurtosis deconvolution (AMCKD) for accurateidentification of compound faults of rolling bearings. The AMCKD method combines the powerful capability of cuckoo search algorithmfor global optimization with the advantage of Maximum correlated kurtosis deconvolution (MCKD) for impact signal extraction. In contrastto traditional methods, such as direct envelop spectrum, Discrete wavelet transform (DWT), and empirical mode decomposition, theproposed method extracts each fault signal related to the single failed part from the compound fault signals and effectively separates thecoupled fault features. First, the original signal is processed using AMCKD method. Demodulation operation is then performed on theobtained single fault signal, and the envelope spectrum is calculated to identify the characteristic frequency information. Verification isperformed on simulated and experimental signals. Results show that the proposed method is more suitable for detecting compound faultsin rolling bearings compared with traditional methods. This research provides a basis for improving the monitoring and diagnosis precisionof rolling bearings.

The electrocatalysis of Mn-Co3O4/CeO2@C particles with different Ce content modified Ti/PbO2 anode and its application for copper electrodeposition

Yin Zihang,He Ruibo,Nie Fei,Wei Zhen,Jia Bo,Feng Qing,Fu Xiaolong,Zhang Wenyan 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.12

The oxygen evolution kinetics of industrial copper electrodeposition is slow, resulting in low electrocatalytic activity and high energy consumption. In this work, a quaternary composite of carbon coated active particles containing Mn, Co and Ce were prepared (Mn-Co3O4/CeO2@C), and Ti/Sb-SnO2/PbO2 electrode doped with these active particles was prepared by co-electrodeposition. The microstructure and chemical composition of the electrode was characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffractometry (XRD). Linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS) and Tafel polarization curve (Tafel) were used to study the electrochemical properties of anode materials. The results showed that the doping of Mn-Co3O4/CeO2@C active particles promoted the crystal transition of PbO2, decreased the average grain size, and the doping of Ce increases the average valence state of Co. The modified titanium electrode showed excellent catalytic activity of the oxygen evolution reaction (OER) characteristics. The overpotential of the doped Ti/Sb-SnO2/PbO2 anode was only 453 mV when the current density was 20 mA cm−2 in 0.5 M H2SO4 solution, which is 508 mV lower than that of the undoped Ti/Sb-SnO2/PbO2 anode. In simulated copper electro-deposition experiments, the cell voltage was reduced by about 400 mV, compared to the undoped Ti/Sb-SnO2/PbO2 electrode.

Isolation and identification of goose skeletal muscle satellite cells and preliminary study on the function of C1q and tumor necrosis factor-related protein 3 gene

Wang Han,He Ke,Zeng Xuehua,Zhou Xiaolong,Yan Feifei,Yang Songbai,Zhao Ayong 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.6

Objective: Skeletal muscle satellite cells (SMSCs) are significant for the growth, regeneration, and maintenance of skeletal muscle after birth. However, currently, few studies have been performed on the isolation, culture and inducing differentiation of goose muscle satellite cells. Previous studies have shown that C1q and tumor necrosis factor-related protein 3 (CTRP3) participated in the process of muscle growth and development, but its role in the goose skeletal muscle development is not yet clear. This study aimed to isolate, culture, and identify the goose SMSCs in vitro. Additionally, to explore the function of CTRP3 in goose SMSCs. Methods: Goose SMSCs were isolated using 0.25% trypsin from leg muscle (LM) of 15 to 20 day fertilized goose eggs. Cell differentiation was induced by transferring the cells to differentiation medium with 2% horse serum and 1% penicillin streptomycin. Immunofluorescence staining of Desmin and Pax7 was used to identify goose SMSCs. Quantitative realtime polymerase chain reaction and western blot were applied to explore developmental expression profile of CTRP3 in LM and the regulation of CTRP3 on myosin heavy chains (MyHC), myogenin (MyoG) expression and Notch signaling pathway related genes expression. Results: The goose SMSCs were successfully isolated and cultured. The expression of Pax7 and Desmin were observed in the isolated cells. The expression of CTRP3 decreased significantly during leg muscle development. Overexpression of CTRP3 could enhance the expression of two myogenic differentiation marker genes, MyHC and MyoG. But knockdown of CTRP3 suppressed their expression. Furthermore, CTRP3 could repress the mRNA level of Notch signaling pathway-related genes, notch receptor 1, notch receptor 2 and hairy/ enhancer-of-split related with YRPW motif 1, which previously showed a negative regulation in myoblast differentiation. Conclusion: These findings provide a useful cell model for the future research on goose muscle development and suggest that CTRP3 may play an essential role in skeletal muscle growth of goose. Objective: Skeletal muscle satellite cells (SMSCs) are significant for the growth, regeneration, and maintenance of skeletal muscle after birth. However, currently, few studies have been performed on the isolation, culture and inducing differentiation of goose muscle satellite cells. Previous studies have shown that C1q and tumor necrosis factor-related protein 3 (CTRP3) participated in the process of muscle growth and development, but its role in the goose skeletal muscle development is not yet clear. This study aimed to isolate, culture, and identify the goose SMSCs <i>in vitro</i>. Additionally, to explore the function of CTRP3 in goose SMSCs.Methods: Goose SMSCs were isolated using 0.25% trypsin from leg muscle (LM) of 15 to 20 day fertilized goose eggs. Cell differentiation was induced by transferring the cells to differentiation medium with 2% horse serum and 1% penicillin streptomycin. Immunofluorescence staining of Desmin and Pax7 was used to identify goose SMSCs. Quantitative realtime polymerase chain reaction and western blot were applied to explore developmental expression profile of CTRP3 in LM and the regulation of CTRP3 on myosin heavy chains (MyHC), myogenin (MyoG) expression and Notch signaling pathway related genes expression.Results: The goose SMSCs were successfully isolated and cultured. The expression of <i>Pax7</i> and Desmin were observed in the isolated cells. The expression of CTRP3 decreased significantly during leg muscle development. Overexpression of CTRP3 could enhance the expression of two myogenic differentiation marker genes, <i>MyHC</i> and <i>MyoG</i>. But knockdown of CTRP3 suppressed their expression. Furthermore, CTRP3 could repress the mRNA level of Notch signaling pathway-related genes, notch receptor 1, notch receptor 2 and hairy/enhancer-of-split related with YRPW motif 1, which previously showed a negative regulation in myoblast differentiation.Conclusion: These findings provide a useful cell model for the future research on goose muscle development and suggest that CTRP3 may play an essential role in skeletal muscle growth of goose.

MiR-126-3p inhibits apoptosis and promotes proliferation by targeting phosphatidylinositol 3-kinase regulatory subunit 2 in porcine ovarian granulosa cells

Zhou, Xiaofeng,He, Yingting,Jiang, Yao,He, Bo,Deng, Xi,Zhang, Zhe,Yuan, Xiaolong,Li, Jiaqi Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.6

Objective: Numerous studies have indicated that the apoptosis and proliferation of granulosa cells (GCs) are closely related to the normal growth and development of follicles and ovaries. Previous evidence has suggested that miR-126-3p might get involved in the apoptosis and proliferation of GCs, and phosphatidylinositol 3-kinase regulatory subunit 2 (PIK3R2) gene has been predicted as one target of miR-126-3p. However, the molecular regulation of miR-126-3p on PIK3R2 and the effects of PIK3R2 on porcine GCs apoptosis and proliferation remain virtually unexplored. Methods: In this study, using porcine GCs as a cellular model, luciferase report assay, mutation and deletion were applied to verify the targeting relationship between miR-126-3p and PIK3R2. Annexin-V/PI staining and 5-ethynyl-2'-deoxyuridine assay were applied to explore the effect of PIK3R2 on GCs apoptosis and proliferation, respectively. Real-time quantitative polymerase chain reaction and Western Blot were applied to explore the regulation of miR-126-3p on PIK3R2 expression. Results: We found that miR-126-3p targeted at PIK3R2 and inhibited its mRNA and protein expression. Knockdown of PIK3R2 significantly inhibited the apoptosis and promoted the proliferation of porcine GCs, and significantly down-regulated the mRNA expression of several key genes of PI3K pathway such as insulin-like growth factor 1 receptor (IGF1R), insulin receptor (INSR), pyruvate dehydrogenase kinase 1 (PDK1), and serine/threonine kinase 1 (AKT1). Conclusion: MiR-126-3p might target and inhibit the mRNA and protein expressions of PIK3R2, thereby inhibiting GC apoptosis and promoting GC proliferation by down-regulating several key genes of the PI3K pathway, IGF1R, INSR, PDK1, and AKT1. These findings would provide great insight into further exploring the molecular regulation of miR-126-3p and PIK3R2 on the functions of GCs during the folliculogenesis in female mammals.

One Step Fabrication of Micro-hole on SiCp/Al Cambered Surface by Micro-EDM and Mechanical-reaming Combined Machining

Sirui Gong,Han Wang,Xiaolong He,Zhenlong Wang,Yukui Wang 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.10 No.4

SiCp/Al, as a new material, is widely used for its excellent wear resistance, corrosion resistance and other properties. Micro-holes are important but difficult to obtain on this ceramic material especially in curved surface. In this paper, taking micro bearing bush as an example, a micro-EDM and in-situ micro-reaming method is put forward for preparing micro through holes and micro eccentric holes on SiCp/Al cambered surface, without secondary clamping or tool change. High machining efficiency, low wear and no recasting layer can be achieved by this method. Here, machining characteristics is studied in hole micro-EDM on SiCp/Al, small taper and high efficiency machining is realized by comparing four electrical discharge drilling technique. Carbon deposition and tool wear are related to electrical parameters according to experimental exploration. After determining the discharge gap, taper and recast layer thickness, a segmented tool is designed, which can use the lower part to perform micro-EDM with the taper of 1.04°, MRR is 0.069 mm3/min, and the TWR is 3.22%. Then only needs to continue feeding the tool, the upper part of the tool can be used to achieve reaming, which can fully remove the recast layer, and decreases the roughness of hole wall from Ra 1.452 μm to Ra 0.803 μm.

Effects of shear keys on seismic performance of an isolation system

Biao Wei,Chaobin Li,Xiaolong Jia,Xuhui He,Menggang Yang 국제구조공학회 2019 Smart Structures and Systems, An International Jou Vol.24 No.3

The shear keys are set in a seismic isolation system to resist the long-term service loadings, and are cut off to isolate the earthquakes. This paper investigated the influence of shear keys on the seismic performance of a vertical spring-viscous damper-concave Coulomb friction isolation system by an incremental dynamic analysis (IDA) and a performance-based assessment. Results show that the cutting off process of shear keys should be simulated in a numerical analysis to accurately predict the seismic responses of isolation system. Ignoring the cutting off process of shear keys usually leads to untrue seismic responses in a numerical analysis, and many of them are unsafe for the design of isolated structure. And those errors will be increased by increasing the cutting off force of shear keys and decreasing the spring constant of shear keys, especially under a feeble earthquake. The viscous damping action postpones the cutting off time of shear keys during earthquakes, and reduces the seismic isolation efficiency. However, this point can be improved by increasing the spring constant of shear keys.

Optimization of Nutrients for Dinactin Production by a Marine Streptomyces sp. from the High Latitude Arctic

Jiao Zhou,Hao He,Xiaolong Wang,Jian Lu,Xiangshan Zhou,Menghao Cai,YuanXingZhang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4

Streptomyces sp. R-527F, which produces the macrotetrolide antibiotic dinactin, was isolated from the sediments of the Arctic Ocean. In this work, optimization of the nutrients required for dinactin production including medium development and precursor stimulation, were investigated. Optimization of the medium and replacement of polar sea water were achieved using a one factor at a time experiment in conjunction with statistical analysis using methods covering Plackett–Burman design, the steepest descent method and central composite design. Dinactin production in the optimized medium was 160.8 mg/L, which was 47 fold higher than the control. Supplementation of the fermentation with exogenous acetate (1.5 mmol/L), succinate (6 mmol/L), malonate (24 mmol/L) and citrate (6 mmol/L) further enhanced dinactin biosynthesis by 42.7, 122.3, 66.7, and 62.1%, respectively. The precursors, in particular succinate, facilitated sugar use and also increased pH levels. Furthermore, a six-pulse feeding of total 6 mmol/L succinate in a 5 L bioreactor fermentation yielded a maximal production of 279.0 mg/L dinactin, 124.1% higher than that without precursor stimulation. This nutritional regulation process is easy to scale up and holds the potential for adaptation to industrial use. Keywords: arctic actinomycete, fermentation, dinactin