http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Factors Influencing the Efficiency of In Vitro Embryo Production in the Pig
Tao Lin,Jae Eun Lee,Hyun Young Shin,,Reza K. Oqani,Dong Il Jin 한국동물생명공학회(구 한국동물번식학회) 2015 Reproductive & developmental biology Vol.39 No.2
Pigs are considered an ideal source of human disease model due to their physiological similarities to humans. However, the low efficiency of in vitro embryo production (IVP) is still a major barrier in the production of pig offspring with gene manipulation. Despite ongoing advances in the associated technologies, the developmental capacity of IVP pig embryos is still lower than that of their in vivo counterparts, as well as IVP embryos of other species (e.g., cattle and mice). The efficiency of IVP can be influenced by many factors that affect various critical steps in the process. The previous relevant reviews have focused on the in vitro maturation system, in vitro culture conditions, in vitro fertilization medium, issues with polyspermy, the utilized technologies, etc. In this review, we concentrate on factors that have not been fully detailed in prior reviews, such as the oocyte morphology, oocyte recovery methods, denuding procedures, first polar body morphology and embryo quality.
Tao Lin,Reza K. Oqani,Jae Eun Lee,So Yeon Kim,Dong Il Jin 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
SETD2 (SET domain containing protein 2) is known as a histone H3 lysine 36 (H3K- 36)-specific methyl-transferase, and suggesting that it has an important role in gene active transcription in human cells. In the current study, to investigate the dynamic change of SETD2 in pig, we determined the SETD2 expression in porcine fetal fibroblasts, oocytes and preimplantation embryos derived from in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) by immunofluorescence using specific antibodies and laser scanning confocal microscopy. In porcine fetal fibroblasts, SETD2 expression was detected in inter phase, not in M (mitosis) phase. SETD2 signal was observed in non-surrounded nucleolus (NSN) stage oocytes. However, there were no signals were detected in surrounded nucleolus (SN), metaphase I (MI), and metaphase II (MII) stage oocytes. In IVF embryos, SETD2 signal was detectable in sperm, but this signal was lost after fertilization, and then became detectable at 2-cell stage, peaked at the 4-cell stage which is porcine embryonic gene activation time. Similar to the pattern found in IVF embryos, SETD2 signal in PA embryo was not detected at 1-cell stage, but detected at 2-cell stage, and maintained to blastocyst stage. Interestingly, unlike IVF and PA embryos, SETD2 signal could not be lost in 1-cell stage of SCNT embryos, and this signal was detectable in whole SCNT embryonic developmental stage. Overall, these data indicated that the SETD2 may be a mark of embryonic gene activation in porcine preimplantation embryos. Aberrant SETD2 expression occur in 1-cell stage of porcine SCNT embryos may be a factor of inducing low of cloning.
Low Image Distortion Constrained Power Saving for OLED Displays
Lin-Tao Duan,Bing Guo,Yan Shen,Ji-He Wang,Wen-Li Zhang 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.11
Organic Light Emitting Diode (OLED) displays have matured into current smartphones. How to prolong the lifetime of displays while preserving the display quality becomes a primary issue. In this paper, we propose a low image distortion constrained power-saving approach for OLED displays based on gamma correction and saturation scaling. We first investigate the impact of gamma correction and saturation scaling on the power of emissive displays. The results show that changing the gamma and saturation value can obtain lower display power consumption when original image color maps to another one. Thus, we integrate the gamma correction and the saturation scaling into a new low-power approach for OLED displays. However, low gamma and high saturation lead to distortion on displaying. To guarantee user experience in this paper, the CIEDE2000 color difference formula and the Mean Structural Similarity Index (MSSIM) are used to evaluate the effectiveness of our approach. The results show that our approach saves up significant display power with high image quality.
Factors Influencing the Efficiency of In Vitro Embryo Production in the Pig
Tao Lin,Jae Eun Lee,Hyun Young Shin,Reza K. Oqani,Dong Il Jin 한국동물번식학회 2015 Reproductive & developmental biology Vol.39 No.2
Pigs are considered an ideal source of human disease model due to their physiological similarities to humans. However, the low efficiency of in vitro embryo production (IVP) is still a major barrier in the production of pig offspring with gene manipulation. Despite ongoing advances in the associated technologies, the developmental capacity of IVP pig embryos is still lower than that of their in vivo counterparts, as well as IVP embryos of other species (e.g., cattle and mice). The efficiency of IVP can be influenced by many factors that affect various critical steps in the process. The previous relevant reviews have focused on the in vitro maturation system, in vitro culture conditions, in vitro fertilization medium, issues with polyspermy, the utilized technologies, etc. In this review, we concentrate on factors that have not been fully detailed in prior reviews, such as the oocyte morphology, oocyte recovery methods, denuding procedures, first polar body morphology and embryo quality.
Lin, Tao,Lee, Jae Eun,Kang, Jung Won,Shin, Hyeon Yeong,Lee, Ju Bin,Jin, Dong Il MDPI 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.2
<P>Mammalian oocytes and early embryos derived from in vitro production are highly susceptible to a variety of cellular stresses. During oocyte maturation and preimplantation embryo development, functional proteins must be folded properly in the endoplasmic reticulum (ER) to maintain oocyte and embryo development. However, some adverse factors negatively impact ER functions and protein synthesis, resulting in the activation of ER stress and unfolded protein response (UPR) signaling pathways. ER stress and UPR signaling have been identified in mammalian oocytes and embryos produced in vitro, suggesting that modulation of ER stress and UPR signaling play very important roles in oocyte maturation and the development of preimplantation embryos. In this review, we briefly describe the current state of knowledge regarding ER stress, UPR signaling pathways, and their roles and mechanisms in mammalian (excluding human) oocyte maturation and preimplantation embryo development.</P>
Factors Influencing the Efficiency of In Vitro Embryo Production in the Pig
Lin, Tao,Lee, Jae Eun,Shin, Hyun Young,Oqani, Reza K.,Jin, Dong Il The Korean Society of Animal Reproduction 2015 Reproductive & developmental biology Vol.39 No.2
Pigs are considered an ideal source of human disease model due to their physiological similarities to humans. However, the low efficiency of in vitro embryo production (IVP) is still a major barrier in the production of pig offspring with gene manipulation. Despite ongoing advances in the associated technologies, the developmental capacity of IVP pig embryos is still lower than that of their in vivo counterparts, as well as IVP embryos of other species (e.g., cattle and mice). The efficiency of IVP can be influenced by many factors that affect various critical steps in the process. The previous relevant reviews have focused on the in vitro maturation system, in vitro culture conditions, in vitro fertilization medium, issues with polyspermy, the utilized technologies, etc. In this review, we concentrate on factors that have not been fully detailed in prior reviews, such as the oocyte morphology, oocyte recovery methods, denuding procedures, first polar body morphology and embryo quality.
Effects of sorbitol on porcine oocyte maturation and embryo development <i>in vitro</i>
Lin, Tao,Zhang, Jin Yu,Diao, Yun Fei,Kang, Jung Won,Jin, Dong-Il Cambridge University Press 2015 Zygote Vol.23 No.2
<B>Summary</B><P>In the present study, a porcine system was supplemented with sorbitol during <I>in vitro</I> maturation (IVM) or <I>in vitro</I> culture (IVC), and the effects of sorbitol on oocyte maturation and embryonic development following parthenogenetic activation were assessed. Porcine immature oocytes were treated with different concentrations of sorbitol during IVM, and the resultant metaphase II stage oocytes were activated and cultured in porcine zygote medium-3 (PZM-3) for 7 days. No significant difference was observed in cumulus expansion and the nuclear maturation between the control and sorbitol-treated groups, with the exception of the 100 mM group, which showed significantly decreased nuclear maturation and cumulus expansion. There was no significant difference in the intracellular reactive oxygen species (ROS) levels between oocytes matured with 10 or 20 mM sorbitol and control groups, but 50 and 100 mM groups had significantly higher ROS levels than other groups. The 20 mM group showed significant increases in intracellular glutathione and subsequent blastocyst formation rates following parthenogenetic activation compared with the other groups. During IVC, supplementation with sorbitol significantly reduced blastocyst formation and increased the apoptotic index compared with the control. The apoptotic index of blastocysts from the sorbitol-treated group for entire culture period was significantly higher than those of the partially sorbitol-exposed groups. Based on these findings, it can be concluded that the addition of a low concentration of sorbitol (20 mM) during IVM of porcine oocytes benefits subsequent blastocyst development and improves embryo quality, whereas sorbitol supplement during IVC has a negative effect on blastocyst formation.</P>