X500R QTOF System with SWATH<SUB>®</SUB> Acquisition for Pesticide Residue Screening in Fruits and Vegetables

Xiaojing Sun,Haiyan Cheng,Lijun Li,Wenhai Jin,Kim, Woobok(김우복) 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.4

Parametric study of an undulating plate in a power-extraction regim

Xiaojing Sun,Jihua Zhang,Huizhen Li,Diangui Huang 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.1

Our previous studies suggest that instead of propulsion, a body undergoing lateral traveling-wave-like motions can also work like a kinetic energy harvester which extracts energy from moving fluids including wind and water currents. Parameters including wavelength, dimensionless wave velocity and amplitude have critical effects on the energy extraction efficiencies of this type of undulating foil energy harvester. In this paper, a two-dimensional, numerical simulation of a flexible plate undergoing a traveling wave motion was then conducted. At a given dimensionless wave speed, it is found that there exits an optimum wavelength at which this type of energy harvester can extract the maximum amount of kinematic energy from the flow. Moreover, the optimum value of the wavelength increases as amplitude increases. A high efficiency area appears under the optimal combination of wavelength and amplitude. At a given amplitude, the optimal dimensionless wave speed for maximum power extraction decreases with increasing wavelength. The high efficiency area of the undulating plate resulting from the optimal combination of wavelength and wave speed is identified. At a given wave length, there is an optimal value of amplitude at which the maximum energy extraction can be achieved. In this case, a high efficiency area representing the optimal combination of amplitude and wave speed has also been discovered.

Thermosensitive Gold Nanoparticles Based on Star-Shaped Poly(N-isopropylacrylamide) with a Cubic Silsesquioxane Core

Xiaojing Zhang,Yuelei Hu,Ruixue Liu,Jiashu Sun,Shaoming Fang 한국고분자학회 2015 Macromolecular Research Vol.23 No.3

Study on the splitting failure of the surrounding rock of underground caverns

Li, Xiaojing,Chen, Han-Mei,Sun, Yanbo,Zhou, Rongxin,Wang, Lige Techno-Press 2018 Geomechanics & engineering Vol.14 No.5

In this paper splitting failure on rock pillars among the underground caverns has been studied. The damaged structure is considered to be thin plates and then the failure mechanism of rock pillars has been studied consequently. The critical load of buckling failure of the rock plate has also been obtained. Furthermore, with a combination of the basic energy dissipation principle, generalized formulas in estimating the number of splitting cracks and in predicting the maximum deflection of thin plate have been proposed. The splitting criterion and the mechanical model proposed in this paper are finally verified with numerical calculations in FLAC 3D.

TsMIP6 enhances the tolerance of transgenic rice to salt stress and interacts with target proteins

Linlin Sun,Guohong Yu,Xiaori Han,Shichao Xin,Xiaojing Qiang,Linlin Jiang,Shuhui Zhang,Xian-guo Cheng 한국식물학회 2015 Journal of Plant Biology Vol.58 No.5

Aquaporins (AQPs), a large family of channel proteins in plants, play an important role in regulating the balance of osmotic potential in cells. We isolated an AQP gene, TsMIP6, from the halophyte Thellungiella salsuginea and functionally characterized it in transgenic rice (Oryza sativa). This gene belongs to a subfamily of tonoplast intrinsic proteins and is localized at the plasma membrane. Real-time PCR showed that expression of TsMIP6 in shoots or roots of T. salsuginea was markedly induced by salinity, whereas its ectopic expression in ‘Kitaake’ lines of rice significantly increased plant tolerance to salt stress. Physiological data suggested that TsMIP6 is involved in regulating ion homeostasis and water channel activity in salt-stressed transgenic rice. Heterologous expression analysis indicated that TsMIP6 specifically interacts with a member of the glycoside hydrolase family 64 protein #617 in yeast cells. This suggests that the relationship between TsMIP6 and #617 has a crucial role in mediating osmotic balance in plant cells. Moreover, TsMIP6 might help to modulate the transport of some neutral molecules and may function through a pathway regulating solute equilibrium to maintain osmotic potential.

A variational nodal formulation for multi-dimensional unstructured neutron diffusion problems

Qizheng Sun,Wei Xiao,Xiangyue Li,Han Yin,Tengfei Zhang,Xiaojing Liu Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.6

A variational nodal method (VNM) with unstructured-mesh is presented for solving steady-state and dynamic neutron diffusion equations. Orthogonal polynomials are employed for spatial discretization, and the stiffness confinement method (SCM) is implemented for temporal discretization. Coordinate transformation relations are derived to map unstructured triangular nodes to a standard node. Methods for constructing triangular prism space trial functions and identifying unique nodes are elaborated. Additionally, the partitioned matrix (PM) and generalized partitioned matrix (GPM) methods are proposed to accelerate the within-group and power iterations. Neutron diffusion problems with different fuel assembly geometries validate the method. With less than 5 pcm eigenvalue (k_eff) error and 1% relative power error, the accuracy is comparable to reference methods. In addition, a test case based on the kilowatt heat pipe reactor, KRUSTY, is created, simulated, and evaluated to illustrate the method's precision and geometrical flexibility. The Dodds problem with a step transient perturbation proves that the SCM allows for sufficiently accurate power predictions even with a large time-step of approximately 0.1 s. In addition, combining the PM and GPM results in a speedup ratio of 2-3.

Small molecules enable OCT4-mediated direct reprogramming into expandable human neural stem cells

Zhu, Saiyong,Ambasudhan, Rajesh,Sun, Woong,Kim, Hyun Jung,Talantova, Maria,Wang, Xiaojing,Zhang, Mingliang,Zhang, Yu,Laurent, Timothy,Parker, James,Kim, Han-Seop,Zaremba, Jeffrey D,Saleem, Sofiyan,San Springer Science and Business Media LLC 2014 Cell research Vol.24 No.1

Vibration response and parameter influence of TBM cutterhead system under extreme conditions

Jingxiu Ling,Wei Sun,Xiaojing Yang,Xin Tong,Ning Zhang 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.10

A cutterhead endures complex random loads when the TBM tunnels, which leads to structure excessive vibration, due to the extreme working conditions. So anti-vibration design is key in the cutterhead system design. In this study we established a virtual prototype of TBM mainframe system, comprehensively considering the spatial multi-point random excitations, split cutterhead structure and multidirectional support stiffness of the main bearing, etc., based on multi-body system dynamics and virtual prototype technology. For verifying the virtual prototype model, a TBM cutterhead vibration field detection system was constructed, to test the cutterhead accelerations and compare the simulation and measured results. The results show that the acceleration change rule by the two approaches is similar, and the maximum relative error is 55 %, keeping on the same order of magnitude, which illustrates the established model has certain reliability. Accordingly, an orthogonal test scheme for cutterhead radial vibration analysis was designed, to analyze the influence of different parameters on cutterhead vibration, based on the virtual prototype model. As indicated in the analysis, the parameter of cutter force had the greatest influence on cutterhead vibration, and then the pinion support stiffness and cutterhead rotating speed. Last, the cutterhead vibration optimal schemes in different directions were confirmed. The research results and methods can provide reference for TBM system parameters matching.

Molecular identification of DNA barcoding of Leguminous toxic species and quantitative analysis by ELISA kits

Wang Jie,Wang Shuangyu,Sun Fenglin,Liu Chang,Zhao Jinquan,Yu Hongwei,Lv Xiaojing,Liu Ze,Bu Shuhua,Yu Weisen 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.2

Some edible Leguminous are toxic when raw, and the Chinese are particularly fond of beans, so Leguminous poisoning is very common in China. Rapid and accurate identification of poisoned species and determination of their toxic components would better assist physicians in treating patients. However, traditional morphology-based identification methods possess many limitations. DNA barcoding technique is a new species identification technique developed in recent years, which is expected to make up for the shortcomings of traditional morphological identification. In this study, a comprehensive evaluation system based on DNA barcoding and ELISA kits was attempted. A total of 30 Leguminous toxic plants were collected, involving 9 genera and 10 species. We used simulated gastric fluid (SGF) to simulate the human gastric environment. Three markers (rbcL, trnH-psbA, and ITS) were amplified and sequenced for all untreated and 15 mock-digested samples. The validity of DNA barcoding for species identification was assessed using the Basic Local Alignment Search Tool (BLAST) method and the tree construction method. The levels of three toxic components (saponin, phytoagglutin and trasylol) were determined in all samples using ELISA kits. The amplification success rate of all three regions was high (rbcL 96.67%, trnH-psbA 100%, and ITS 100%), but the sequencing of the trnH-psbA region was less satisfactory (66.67%), and SGF had a significant impact on the sequencing of the ITS region (After 40 min of SGF treatment, the sequencing success rate decreased by 46.67%). The samples from different species and origins contained different levels of toxic components, and the levels of all three sub- stances decreased significantly after undergoing SGF digestion. After 1 h of SGF treatment, the saponin content decreased to 0–8.60% in untreated content (PHA decreased to 8.62–36.88%, trasylol decreased to 4.70–47.06%). The current results suggest that DNA barcoding has great potential for rapid identification of Leguminous poisoning in clinical settings. Toxins are probably not detectable in the patient for longer periods of poisoning. We recommend DNA barcoding technology as a first step for rapid screening and combined with toxin analysis for clinical diagnosis.

Virus-like Particles as Antiviral Vaccine: Mechanism, Design, and Application

Lei Zhang,Wen Xu,Xi Ma,XiaoJing Sun,JinBo Fan,Yang Wang 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.1

Virus-like particles (VLPs) are viral structural protein that are noninfectious as they do not contain viral genetic materials. They are safe and effective immune stimulators and play important roles in vaccine development because of their intrinsic immunogenicity to induce cellular and humoral immune responses. In the design of antiviral vaccine, VLPs based vaccines are appealing multifunctional candidates with the advantages such as self-assembling nanoscaled structures, repetitive surface epitopes, ease of genetic and chemical modifications, versatility as antigen presenting platforms, intrinsic immunogenicity, higher safety profile in comparison with live-attenuated vaccines and inactivated vaccines. In this review, we discuss the mechanism of VLPs vaccine inducing cellular and humoral immune responses. We outline the impact of size, shape, surface charge, antigen presentation, genetic and chemical modification, and expression systems when constructing effective VLPs based vaccines. Recent applications of antiviral VLPs vaccines and their clinical trials are summarized.