Expression of Cdc25B mRNA in Duodenal Mucosa of Chicken

Qin, Junhui,Zhang, Hui,Bao, Huijun,Zhou, Qiang,Liu, Yi,Xu, Chunsheng,Chu, Xiaohong,Chen, Qiusheng Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.4

Cdc25B is a mitotic regulator that might act as a starter phosphatase to initiate the positive feedback loop at the entry into mitotic (M) phase. In the present study, distribution of Cdc25B mRNA in duodenal mucosa of the chicken was demonstrated by means of in situ hybridization histochemistry (ISHH) using sense and antisense digoxigenin (DIG)-labeled RNA probes. The results showed that there were many labeled cells distributing in the duodenal mucosa of the adult chicken. Of these labeled cells, 81.60${\pm}$9.63% of Cdc25B mRNA positive cells was distributed in the basilar part and mid-portion of the intestinal gland and 36.21${\pm}$8.81% in the middle and basilar portion of villi of the small intestine of the chicken, respectively. Most of these labeled cells were positive in the regions of the stem cell and proliferation. The signals of ISHH decreased from basilar to upper part in the crypt of Lieberkuhn and weakened in the inferior villi of the duodenum. Moreover, the positive signals were both in the cytoplasm and cell nucleus. However, the labeled cells were negative in both the lamina muscularis mucosae and muscular layer. The results of ISHH suggested the existence of Cdc25B mRNA and vigorous proliferation activities in the duodenal mucosa of adult chicken, replenishing the cells which had sloughed off from the superior part of the villus. Our results provide some molecular evidence for a regular pattern of avian intestinal epitheliosis and functional partition and provide an approach to further study of the locations of Cdc25B in the chicken.

The Expression Patterns of Cdc25A, Cdc25B, Sox2 and Mnb in Central Nervous System in Early Chicken Embryos

Zhang, Hui,Qin, Junhui,Cao, Jingjing,Hei, Nainan,Xu, Chunsheng,Yang, Ping,Liu, Haili,Chu, Xiaohong,Bao, Huijun,Chen, Qiusheng Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.6

The sense and antisense digoxigenin-labeled RNA probes of four genes, Cdc25A, Cdc25B, Sox2 and Mnb, were produced by using SP6 and T7 RNA polymerases, respectively, and in vitro transcription. Expression patterns of the four genes were detected by in situ hybridization in HH (Hamburger and Hamilton) stage 10 chick embryos. In general, expression patterns of the four genes were similar. mRNA of the four genes was mostly restricted to the entire CNS (central nervous system). All were confined to an identical region, neural tube, neural groove and caudal neural plate, corresponding to the notochord or spinal cord, but there was some distinction in specific region or in concentration, for example in somites. The overlap in expression at the same developmental stage in the CNS suggests that the four genes may be functional similar or related in CNS development. Expression patterns of the four genes support specific roles of these regulators in the developing CNS.

Weak magnetic field accelerates chromate removal by zero-valent iron

Feng, Pian,Guan, Xiaohong,Sun, Yuankui,Choi, Wonyong,Qin, Hejie,Wang, Jianmin,Qiao, Junlian,Li, Lina Elsevier 2015 Journal of environmental sciences Vol.31 No.-

<P>Weak magnetic field (WMF) was employed to improve the removal of Cr(VI) by zero-valent iron (ZVI) for the first time. The removal rate of Cr(VI) was elevated by a factor of 1.12-5.89 due to the application of a WMF, and the WMF-induced improvement was more remarkable at higher Cr(VI) concentration and higher pH. Fe2+ was not detected until Cr(VI) was exhausted, and there was a positive correlation between the WMF-induced promotion factor of Cr(VI) removal rate and that of Fe2+ release rate in the absence of Cr(VI) at pH4.0-5.5. These phenomena imply that ZVI corrosion with Fe2+ release was the limiting step in the process of Cr(VI) removal. The superimposed WMF had negligible influence on the apparent activation energy of Cr(VI) removal by ZVI, indicating that WMF accelerated Cr(VI) removal by ZVI but did not change the mechanism. The passive layer formed with WMF was much more porous than without WMF, thereby facilitating mass transport. Therefore, WMF could accelerate ZVI corrosion and alleviate the detrimental effects of the passive layer, resulting in more rapid removal of Cr(VI) by ZVI. Exploiting the magnetic memory of ZVI, a two-stage process consisting of a small reactor with WMF for ZVI magnetization and a large reactor for removing contaminants by magnetized ZVI can be employed as a new method of ZVI-mediated remediation.</P>

Exploring Flow Characteristics in IPv6: A Comparative Measurement Study with IPv4 for Traffic Monitoring

( Qiang Li ),( Tao Qin ),( Xiaohong Guan ),( Qinghua Zheng ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.4

With the exhaustion of global IPv4 addresses, IPv6 technologies have attracted increasing attentions, and have been deployed widely. Meanwhile, new applications running over IPv6 networks will change the traditional traffic characteristics obtained from IPv4 networks. Traditional models obtained from IPv4 cannot be used for IPv6 network monitoring directly and there is a need to investigate those changes. In this paper, we explore the flow features of IPv6 traffic and compare its difference with that of IPv4 traffic from flow level. Firstly, we analyze the differences of the general flow statistical characteristics and users` behavior between IPv4 and IPv6 networks. We find that there are more elephant flows in IPv6, which is critical for traffic engineering. Secondly, we find that there exist many one-way flows both in the IPv4 and IPv6 traffic, which are important information sources for abnormal behavior detection. Finally, in light of the challenges of analyzing massive data of large-scale network monitoring, we propose a group flow model which can greatly reduce the number of flows while capturing the primary traffic features, and perform a comparative measurement analysis of group users` behavior dynamic characteristics. We find there are less sharp changes caused by abnormity compared with IPv4, which shows there are less large-scale malicious activities in IPv6 currently. All the evaluation experiments are carried out based on the traffic traces collected from the Northwest Regional Center of CERNET (China Education and Research Network), and the results reveal the detailed flow characteristics of IPv6, which are useful for traffic management and anomaly detection in IPv6.

Investigation on Air Texturing Process for Diacetate Blending with Polyester Filaments

Zhang, Jing,Zhang, Zhilong,Wang, Shanyuan,Qin, Xiaohong The Korean Fiber Society 2007 Fibers and polymers Vol.8 No.4

Influences of processing parameters on tensile property, stability and bulk of core-and-effect air textured yarns of diacetate polyester filaments are mainly examined in this paper. When the air pressure is raised, the tenacity and breaking elongation of textured yarns are reduced, Instability I and II tend to decrease at first and then increase, the core bulk declines markedly at first and then changes slowly, whereas the overall bulk changes little at first and then goes up greatly. With increase in texturing speed, the yarn tenacity and breaking elongation both drop initially then begin to increase, the core bulk and overall bulk are almost linearly increased, while the yarn instability changes with an unclear trend. When the winding underfeed ratio is increased, the yarn tenacity, breaking elongation and core bulk are reduced, but the yarn stability is slightly improved. The wetting of the core component produces higher tenacity, breaking elongation, instability and bulk, compared with that of the effect component or that of both, but the difference is insignificant.

Printing direction dependence of mechanical behavior of additively manufactured 3D preforms and composites

Quan, Zhenzhen,Suhr, Jonghwan,Yu, Jianyong,Qin, Xiaohong,Cotton, Chase,Mirotznik, Mark,Chou, Tsu-Wei Elsevier 2018 Composite structures Vol.184 No.-

<P><B>Abstract</B></P> <P>Among the processing parameters of additive manufacturing, printing direction is of critical importance. While studies on effects of printing direction have so far mainly focused on mechanical properties of solid specimens, the present research is intended to demonstrate printing direction dependence of mechanical behavior of additively manufactured 3D preforms and their composites. Compressive behavior of additively manufactured 3D braid preforms and composites was investigated for three distinct printing directions (0°, 45° and Z-direction). Fused filament fabrication (FFF) of acrylonitrile-butadiene-styrene (ABS) filament and short carbon fiber/ABS (CF/ABS) filament was adopted. First, solid cube specimens were fabricated; the parts printed along 0° and 45° directions showed more fabrication-induced pores. Then, 3D braid preforms were fabricated and infused with silicone matrix. For preforms printed along 45° direction, inter-yarn adhesion was observed, which enhanced specimen initial modulus. On the other hand, Z-direction specimens showed higher structural ductility, due to inter-yarn slippage.</P>

Detecting the origin of luminescence in Er³⁺-doped hexagonal Na_1.5Gd_1.5F₆ phosphors

Wang, Xiangfu,Bu, Yanyan,Yan, Xiaohong,Cai, Peiqing,Wang, Jing,Qin, Lin,Vu, Thiquynh,Seo, Hyo Jin OPTICAL SOCIETY OF AMERICA 2016 Optics letters Vol.41 No.22

<P>Understanding site-selective fluorescence is one of valuable importance for spectrum modulation. In this Letter, we observed the existence of two non-equivalent Gd-activated crystallographic sites in an Er3+-doped hexagonal Na1.5Gd1.5F6 phosphor. It is proved that two green emissions from the S-4(3/2) level separately originate from the Gd1 (540 nm) and Na2/Gd2 (550-555 nm) crystallographic sites, and the 657 nm red emission from the F-4(9/2) level only originates from Na2/Gd2 site through using the time-resolved luminescence spectra. The 142.2% absolute enhancement of the red emission is realized through the synergistic effect of ultraviolet downconversion and infrared upconversion induced by the 370 nm and 1.54 mu m dual-mode excitation. (C) 2016 Optical Society of America</P>

Preparation of cMWCNTs-incorporated Hollow Fiber Membrane with Improved Water Flux and Dye Adsorption Properties

Wenying Shi,Hongbin Li,Qiyun Du,Shuo Wang,Haixia Zhang,Xiaohong Qin 한국섬유공학회 2019 Fibers and polymers Vol.20 No.2

Carboxylated multi-walled carbon nanotubes (cMWCNTs) were incorporated in membrane matrix during the drywet spinning process to improve membrane performance. Membrane surface composition and morphology were examined by means of FTIR and SEM. The effects of cMWCNTs content on membrane surface hydrophilicity, separation performanceand dye adsorption properties were well investigated and characterized through WCA examination, the cross-flow filtration measurement and the adsorption experiment. The surface hydrophilicity, the water permeability, solute rejection and dye adsorption properties of the cMWNTs-incorporated hollow fiber membranes have been all improved. When cMWNTs content was 0.3 wt%, the cMWNTs-incorporated hollow fiber membrane obtained the obvious enhanced pure water flux (137.2 L·m-2·h-1, at 0.1 MPa) which was 1.5 times as high as that of the original PSF hollow fiber membrane. The preparedcMWCNTs-incorporated membranes are expected to be used in the treatment of textile dyeing wastewater or employed as the supports of the composite membranes.

Continuous biodiesel production from acidic oil using a combination of the acid-, alkali-catalyzed membrane and GO/PVDF separation membrane

Wenying Shi,Tengfei Li,Hongbin Li,Qiyun Du,Haixia Zhang,Xiaohong Qin 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.107 No.-

An integrated process of esterification with phosphotungstic acid/poly (ether sulfone) (PWA/PES) membrane,transesterification with alkalized polysulfone (APSF) membrane and Graphene Oxide/poly (vinylidenefluoride) (GO/PVDF) separation membrane was carried out to produce biodiesel from acidic oil. Atthe first step, the PWA/PES membrane was introduced to catalyze the esterification reaction. The morphology,maximum pore size, porosity and catalytic esterification performance of PWA/PES membranewere investigated. After the esterification, products and reactants without reaction were put into theGO/PVDF separation membrane to soybean oil and other substances. The permeation mixture flux andsoybean oil rejection of the GO/PVDF separation membrane is 597.89 L/m2h and 98.02%, respectively. At the second step, the APSF membrane was added to catalyze the transesterification reaction in whichthe soybean oil reacted with methanol. The results showed that the esterification conversion was 98.6%and transesterification conversion was 91.2%. The main parameters of the biodiesel product obtainedmeet the Chinese Standard (GB/T 20828) and European Standard (EN 14214).

Fiber-microsphere Binary Structured Composite Fibrous Membranes for Waterproof and Breathable Applications

Jiatai Gu,Zhenzhen Quan,Liming Wang,Hongnan Zhang,Ni Wang,Xiaohong Qin,Rongwu Wang,Jianyong Yu 한국섬유공학회 2022 Fibers and polymers Vol.23 No.6

Membranes with the functions of waterproofness and breathability are of great interest due to their huge scientificand commercial value in many practical applications. However, designing such membranes with a desirable performance bysimple and efficient fabrication process remains a tremendous challenge. Herein, novel binary structured composite fibrousmembranes were developed for waterproof and breathable applications via simple one-step multi-jet electrospinningtechnology. This composite membranes were made up of polyvinylidene fluoride (PVDF) and polyacrylonitrile (PAN). PVDF was incorporated into the PAN fiber matrix as a modifier to improve the hydrophobic behavior of fibers. Meanwhile,PVDF microspheres/fibers were employed as building blocks to orderly embed in PAN/PVDF fiber framework, impartingthe composite membranes with fluffy micro-nano hierarchical structures. Consequently, with the integrated features of robusthydrophobicity as well as elaborate fiber-microsphere binary structure, the resultant composite membranes exhibitedsynergistically modest waterproof and breathable performances with the hydrostatic pressure of 18.04 kPa, water vaportransmittance (WVT) rate of 4.65 kg m-2 d-1, and air permeability of 64.19 mm s-1. This simple preparation process andunique composite structure may provide a new insight to design and fabricate waterproof and breathable membranes forvariously potential applications, including the protective clothing, separation membrane, and filter.