Application of Smart Technology in the Integrated Environmental Management of Urban Wetland Park

Xiaodan Zhao,Shaoping Wang 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.7

Wetland Park is one of the important ecological infrastructures. Besides providing the ecosystem service function and value, it is also an important place for leisure and entertainment. Efficient environmental management is an important measure to promote the sustainable development of the wetland park. By combining the smart technology with routine management method, the efficiency of the environmental management for the urban wetland park can be improved. The application of smart technology set the users' requirements as the basic goal, which is divided into four layers: perception layer, transport layer, support layer and application layer. The perception layer consists of sensor, camera, RFID, mobile terminal device and so on; the transport layer is composed of optical fiber, WIFI and 3G network; the support layer is composed of data management system, information open platform and service support system; and the application layer is composed of four modules including panoramic-view park, environmental maintenance, smart tourism and public participation. A wetland park located in Zhangzhou city, Fujian, China, was taken as a practice case.

MiR-1297 and MiR-26a-5p Inhibit Cell Progression of Keratinocytes in Cholesteatoma Depending on the Regulation of BMI1

Xiaodan Zhu,Fanglei Ye,Shaojuan Hao,Qiuning Yu,Yang Wang,Weihua Lou,Kun Zhao,Hongmin Li 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.1

Cholesteatoma is a pathologically benign but clinically destructive middle ear disease characterized by hyperproliferative keratinocytes. B-cell-specific Moloney murine leukemia virus insertion site 1 (BMI1) has been reported to be upregulated in cholesteatoma tissues. This study aimed to explore the biological role and underlying mechanisms of BMI1 in the progression of cholesteatoma. The expression levels of microRNA (miR)-1297, miR-26a- 5p, and BMI1 in cholesteatoma tissues and cells were examined by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Functional experiments were performed by CCK-8 assay for cell proliferation viability, 5-ethynyl-2'deoxyuridine (EdU) incorporation assay for DNA biosynthesis, colony formation assay for cloning forming ability analysis, transwell assay and wound healing assay for cell metastasis, flow cytometry for cell cycle distribution and cell apoptosis. The protein expression of apoptosis-associated proteins was investigated by western blot. Dual-luciferase reporter assay was conducted to verify the interaction between miR-1297 or miR-26a-5p and BMI1. BMI1 was highly expressed in cholesteatoma tumor tissues. Functional analyses showed that BMI1 knockdown could inhibit the proliferation, colony formation, migration, invasion, cell cycle progression and promoted the apoptosis of keratinocytes. Mechanically, BMI1 was a target of miR-1297 and miR-26a-5p. Moreover, the rescue experiments presented that BMI1 addition could abolish the suppressive effects of miR-1297 or miR-26a-5p overexpression on cell malignant behaviors in keratinocytes. BMI1 could exert an oncogenic role in the malignant development of cholesteatoma through serving as the targets of miR-1297 and miR-26a-5p, which might provide novel strategies for cholesteatoma treatment.

Sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial HK-2 cells by regulating the SIRT1/NF-κB signaling pathway

Xiaodan Ma,Zhixin Guo,Wenhua Zhao,Li Chen 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.6

Sweroside is a natural monoterpene derived from Swertia pseudochinensis Hara. Recently, studies have shown that sweroside exhibits a variety of biological activities, such as anti-inflammatory, antioxidant, and hypoglycemic effects. However, its role and mechanisms in high glucose (HG)-induced renal injury remain unclear. Herein, we established a renal injury model in vitro by inducing human renal tubular epithelial cell (HK-2 cells) injury by HG. Then, the effects of sweroside on HK-2 cell activity, inflammation, reactive oxygen species (ROS) production, and epithelial mesenchymal transition (EMT) were observed. As a result, sweroside treatment ameliorated the viability, inhibited the secretion of inflammatory cytokines (TNF-α, IL-1β, and VCAM-1), reduced the generation of ROS, and inhibited EMT in HK-2 cells. Moreover, the protein expression of SIRT1 was increased and the acetylation of p65 NFkB was decreased in HK-2 cells with sweroside treatment. More importantly, EX527, an inhibitor of SIRT1, that inactivated SIRT1, abolished the improvement effects of sweroside on HK-2 cells. Our findings suggested that sweroside may mitigate HGcaused injury in HK-2 cells by promoting SIRT1-mediated deacetylation of p65 NF-kB.

Derivation and Evaluation of Le Chatelier’s Rule for Flammability Limits

Fuman Zhao,Dedy Ng,Xiaodan Gao,Maria Molnarne,Hans J. Pasmanand,M. Sam Mannan 한국위험물학회 2013 한국위험물학회지 Vol.1 No.1

Le Chatelier’s rule is a well-known method for estimating the fuel mixture flammability limit, and this empirical equation works Best for estimating the lower flammability limits. In this paper, a detailed derivation was conducted on lower flammability limits to shed light on the inherent principle residing in this rule. By assuming a constant flame temperature for pure fuels and mixtures during flame propagation, a theoretical evaluation of Le Chatelier’s rule was presented. Results showed that this rule can be extended to non-ambient conditions for lower flammability limit estimations, particularly when the initial fuel/air reaction system is at non-extreme conditions. Meanwhile, when fuel mixture is diluted with inert gases, or at varied oxygen concentrations, Le Chatelier’s rule still works at lower flammability limit conditions. Unlike the lower flammability limit, generalizations of this rule at upper flammability limits turn out to be impossible when using the same reaction mechanism. This is because of the high complexity of combustion kinetics and dynamic variation of convection flow at the upper flammability limits. Moreover, thermal control is a generally accepted principle for governing the combustion reaction at the lower flammability limit; however, we found that this condition does not necessarily valid for all fuels, such as hydrogen. Finally, a modified Le Chatelier’s mixing rule was proposed with the relaxation of constant flame temperature. The findings from this study can be used as guidelines to maximize safety in the process design and operational procedures involving flammable chemicals.

The Usability Evaluation of the Ecological Agriculture Park Website

Yichuan Zhang,Xiaodan Zhao,Lei Feng,Xinzheng Li 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.12

The food quality and safety condition are the important symbols of the national economic development level and the quality of people's lives. Ecological agricultural park strictly controls seedling, producing, processing, storing and distributing links to provide the market with high-quality traceable food. Websites are important ways of the brand marketing of ecological agriculture park. The usability of the websites is directly linked to the benefit of park. This paper establishes the three levels GAHP (Group-Analytic Hierarchy Process) model that consists of index layer, criterion layer and target layer, and the synthetic weight of each index were determined by the four experts. Taking four ecological agriculture park websites as the evaluation cases and selecting 30 users to rate, the comprehensive score of the four websites was obtained after weighting. GAHP method plays collective intelligence, having a relatively high application value for the optimum selection and optimization of the ecological agriculture park website.

A comparative study on chemical composition of total saponins extracted from fermented and white ginseng under the effect of macrophage phagocytotic function

Xiao, Dan,Xiu, Yang,Yue, Hao,Sun, Xiuli,Zhao, Huanxi,Liu, Shuying The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.3

In this study, white ginseng was used as the raw material, which was fermented with Paecilomyces hepiali through solid culture medium, to produce ginsenosides with modified chemical composition. The characteristic chemical markers of the products thus produced were investigated using rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (RRLC-QTOF-MS). Chemical profiling data were obtained, which were then subjected to multivariate statistical analysis for the systematic comparison of active ingredients in white ginseng and fermented ginseng to understand the beneficial properties of ginsenoside metabolites. In addition, the effects of these components on biological activity were investigated to understand the improvements in the phagocytic function of macrophages in zebrafish. According to the established RRLC-QTOF-MS chemical profiling, the contents in ginsenosides of high molecular weight, especially malonylated protopanaxadiol ginsenosides, were slightly reduced due to the fermentation, which were hydrolyzed into rare and minor ginsenosides. Moreover, the facilitation of macrophage phagocytic function in zebrafish following treatment with different ginseng extracts confirmed that the fermented ginseng is superior to white ginseng. Our results prove that there is a profound change in chemical constituents of ginsenosides during the fermentation process, which has a significant effect on the biological activity of these compounds.