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Xiang Yaoxian,Dai Junxi,Li Yao,You Zongqi,Zhang Junpeng,Huang Xinying,Nie Shuqi,Chen Yujie,Xu Lei,Liu Fengming,Jiang Junjian,Xu Jianguang 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Neutrophils are the earliest master inflammatory regulator cells recruited to target tissues after direct infection or injury. Although inflammatory factors are present in muscle that has been indirectly disturbed by peripheral nerve injury, whether neutrophils are present and play a role in the associated inflammatory process remains unclear. Here, intravital imaging analysis using spinning-disk confocal intravital microscopy was employed to dynamically identify neutrophils in denervated muscle. Slice digital scanning and 3D-view reconstruction analyses demonstrated that neutrophils escape from vessels and migrate into denervated muscle tissue. Analyses using reactive oxygen species (ROS) inhibitors and flow cytometry demonstrated that enhanced ROS activate neutrophils after denervation. Transcriptome analysis revealed that the vast majority of neutrophils in denervated muscle were of the CXCR2 subtype and were recruited by CXCL1. Most of these cells gradually disappeared within 1 week via P53-mediated apoptosis. Experiments using specific blockers confirmed that neutrophils slow the process of denervated muscle atrophy. Collectively, these results indicate that activated neutrophils are recruited via chemotaxis to muscle tissue that has been indirectly damaged by denervation, where they function in delaying atrophy.
Inhibition Effects of Pulp on Seed Germination of American Ginseng
Yao Ge Huang(황요각),Xiang Gao Li(이향고),Shu Yu Cui(최수옥),Ji Xiang Yang(양계상),Ren Song Liu(류인송),Hack Seang Kim(김학성) 한국약용작물학회 1997 한국약용작물학회지 Vol.5 No.2
The germination inhibitory effects of American ginseng (Panax quinquefolium Linne) pulp were discussed. The germination inhibitory effects of pulp juice were decreased in a concentration de-pendent manner. When the pulp juice was diluted 0 (original juice), 1, 2, 5, 10, 20, 50 and 100 times, the radicle lengths of the assay plant, Chinese cabbage (Brassica chinensis Linne), showed 0, 0.32, 0.72, 3.13, 4.83, 16.07, 16.73 and 23.50 mm, respectively (CK=25.98 mm) The pulp evidently inhibited the embryo growth in natural fruit. The longer was the duration that the pulp stayed around the seed, the longer was the time course needed for embryo getting free from the inhibitory effects of pulp. When the depulping was performed on the day 0, 15, 30 and 60 after harvest, the time courses needed for embryo extricating the residual inhibitory effects from pulp were 30, 75, 135 and 135 days, respectively. Moreov-er, if the pulp stayed around the seed with time, that would make the seed rotten ratio increase. When the pulp stayed around the seed for 0, 15, 30, 60 and 270 days, the seed rotten ratios were 5.47, 5.71, 19.05, 27.14 and 33.33%, respectively. Therefore, we concluded that the pulp could be included in the inhibitory components which made American ginseng seed get into dormancy.
충적과정중 미국인삼 종자의 생리 및 생화학적 지표에 관한 동태학적 연구 I
Huang, Yao-Ge,Li, Xiang-Gao,Cui, Shu-Yu,Yu, Wen-Bo,Kuang, Ya-Lan,Yan, Jie-kun,Yang, Ji-Xiang,Liu, Ren-Song,Kim, Hack-Seang The Korean Society of Ginseng 1997 Journal of Ginseng Research Vol.21 No.1
Dynamic parameters of biochemistry including the contents of soluble carbohydrate (SC) , crude fat (CF) , fatty arid (FA) and soluble protein (SP) in American send (Panax qufnquelolium L.) seed are reported in the present paper. When embryo ratio (ER) increased from 7.31 to 20.48%, the SC content decreased from 4.08 to 1.16%. After that, SC content changed little. The CF content decreased successively from 34.50 to 14.40% from the beginning to the end during the embryo afterripening. The changes of FA content did not correlate with that of ER and the changing range of SP content was not large during the seed stratification. All of these may provide some information for understanding the dormancy mechanisms of American seng seed.
Xiaochen Liu,Peng Xu,Like Yao,Xiang Li,Chi Pang,Long Yang,Yi Liang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.10
Laser cladding technology was adapted to fabricate Fe-based shape memory alloy/Nb composite coatings with 5 wt. % Nb doping amounts on 304 stainless steel to promote the microhardness and wear resistance. The SEM, XRD, optical profilometer and electrochemical workstation were used to characterize micromorphology, phase and microstructure. Meanwhile, wear and anti-resistance ability were detected. The results showed that Nb, NbC, ε-martensite, α’-martensite and γ-austenite were found in SMA/Nb coating. The microhardness was enhanced because of the solid solution strengthening and second phase strengthening. Also, the shape recovery rate and anti-corrosion property of the SMA/Nb coating are improved as well.
( Jian Yao ),( Qing Long Chen ),( Guo Xiang Zhong ),( Wen Cao ),( An Yu ),( Yu Huan Liu ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.1
Tannase (Tan410) from a soil metagenomic library was immobilized on different supports, including mesoporous silica SBA-15, chitosan, calcium alginate, and amberlite IRC 50. Entrapment in calcium alginate beads was comparatively found to be the best method and was further characterized. The optimum pH of the immobilized Tan410 was shifted toward neutrality compared with the free enzyme (from pH 6.4 to pH 7.0). The optimum temperature was determined to be 45°C for the immobilized enzyme and 30°C for the free enzyme, respectively. The immobilized enzyme had no loss of activity after 10 cycles, and retained more than 90% of its original activity after storage for 30 days. After immobilization, the enzyme activity was only slightly affected by Hg2+, which completely inhibited the activity of the free enzyme. The immobilized tannase was used to remove 80% of tannins from a green tea infusion on the first treatment. The beads were used for six successive runs resulting in overall hydrolysis of 56% of the tannins.
Study on Dormancy Mechanisms of American Ginseng Seed II - Germination Inhibition of Seed Coat
Huang, Yao-Ge,Li, Xiang-Gao,Cui, Shu-Yu,Yang, Ji-Xiang,Liu, Ren-Song,Kim, Hack-Seang The Korean Society of Pharmacognosy 1996 Natural Product Sciences Vol.2 No.2
This paper gives a description about the germination inhibition of American ginseng (Panax quinquefolium L.) seed coat. The existence of seed coat is one of the inhibitory factors which inhibit the embryo growth, particularly during the morphological after-ripening stage. The seed coat can obstruct the water absorption at the beginning of seed stratification, but it can not threaten seed germination. The inhibition of seed coat is not caused by the mechanical fetter neither. However, before splitting the seed coat, the inhibition of seed coat comes from both air-tight character and inhibitors, and after splitting the seed coat, the inhibition may come mainly from the inhibitors.