강제환우와 보존기간이 난질에 미치는 영향

오홍록,이봉덕,이수기,류현덕,유동조 충남대학교 농업과학연구소 2006 농업과학연구 Vol.33 No.1

An experiment was conducted to investigate the effects of forced molting and egg storage time on the various egg qualities. A total of 240 ISA Brown layers (60 wk of age) were employed as the unmolted treatment (Control). Two hundred and forty ISA Brown layers, molted at the age of 55 wk, were used as a forced molting treatment (T1), and the same number and strain of layers, molted at the age of 70 wk. were also used as the another forced molting treatment (T2). A total of 120 eggs were sampled from each treatment, and divided into six sets, 20 eggs per set. These six sets were stored for 1, 3, 6, 9, 12, and 15 days at 18℃ temperature, respectively. Eggs from T1 were collected from laying hens at the age of 68 wk, which started molting at 60 wk of age and achieved 50% egg production at 63 wk of age. Eggs from T2 were collected from hens at 82 wk of age, which started molting at 70 wk of age and achieved 50% egg production at 78 wk of age. The eggshell strength of T1 was significantly (p<0.05) higher than the Control and T2, and the storing periods did not affect the eggshell strength at all. Neither the forced molting nor the storing periods did not exert any consistent effect on the egg weight, eggshell thickness, eggshell color and egg yolk color. The albumin heights of T1 and T2 were significantly (p<0.05) lower than the Control, and it was remarkably reduced gradually as the storage periods increased in all three treatments. The Haugh unit showed very similar trends as the albumin height, indicating that both albumin height and Haugh unit were very much related to each other. In conclusion, the forced molting improves the eggshell strength, but decreases the albumin height and Haugh unit. The storage of eggs also decreases the albumin height and Haugh unit regardless of molting.

Expression of Tumor Necrosis Factor-alpha-induced Protein 8 in Pancreas Tissues and its Correlation with Epithelial Growth Factor Receptor Levels

Liu, Ke,Qin, Cheng-Kun,Wang, Zhi-Yi,Liu, Su-Xia,Cui, Xian-Ping,Zhang, Dong-Yuan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

Tumor necrosis factor (TNF)-alpha-induced protein 8 (TNFAIP8 or TIPE) is a recently identified protein considered to be associated with carcinogenesis. To investigate its expression pattern in pancreatic cancer patients and to analyse its correlation with clinicopathological significance and the expression levels of epithelial growth factor receptor (EGFR), immunohistochemistry was performed to detect the TNFAIP8 and EGFR proteins in pancreatic cancers, pancreatitis tissues, and healthy controls. The results showed stronger staining of TNFAIP8 protein in pancreatic cancer tissues compared with normal pancreas tissue. Furthermore, in 56 patients with pancreatic cancer, the expression levels of TNFAIP8 in patients with low tumor stage was higher than that with high tumor stage, and correlated with tumor staging and lymph node metastasis (P<0.05). Furthermore, TNFAIP8 expression positively correlated with EGFR levels (r=0.671135, P<0.05). These results indicate that TNFAIP8 may play important roles in the progression of pancreatic cancer.

Animal Products and Processing : Effect of Irradiation on the Mixture of Egg White Proteins Responsible for Foaming Property

Xian De Liu,Rong Xiu Han,Dong Il Jin,Soo Kee Lee,Cheo Run Jo 한국동물자원과학회 2009 한국축산학회지 Vol.51 No.6

Research on the Surface Characteristics of Hardened Steel with Variable Chamfer Edge PCBN Insert by High-Speed Hard Turning

Xian-li Liu,Su-Yan Li,Tao Chen,Dao-Yuan Wang 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.2

In this paper, high-speed cutting tests for hardened steel were carried out where PCBN inserts with variable chamfer and fixed chamfer were employed. The aim of the tests was to investigate the influence of cutting speed and tool wear of variable chamfer insert on the machined surface topography, micro-characteristics and micro-hardness, where comparison and analysis of the machined surface properties were also carried out for the surface produced by the fixed chamfer tool. Experimental results showed that machined surface morphologies become more complex and rough, and peaks appear to a trend towards steepness, with the flank wear increase of the variable chamfer tool. At the same time, the thickness of white layer, hardening depth and hardening degree of the machined surface increase dramatically. With the increase in cutting speed of the variable chamfer tool, it is of primary importance to consider phase transition effects caused by thermal load, which will result in increase in the thickness of white layer on the machined surface. Moreover, it will also lead to decrease in the thickness and deformation degree of plastically deformed layer caused by mechanical load. Compared to the fixed chamfer tool, the cutting force generated by the variable chamfer tool is relatively small, where the flank wear area is also smaller and the groove wear marks is shallower. Therefore, better surface roughness can be obtained by utilizing the variable chamfer tool. In particular, the gap between the roughness of the machined surface formed by two tools becomes more pronounced in the scenarios of high speed and severe wear. Moreover, the thickness of white layer, the depth and degree of hardened layer are relatively small for the variable chamfer tool. Therefore, the surface properties produced by the variable chamfer tool would be more desirable.

Senescence as A Consequence of Ginsenoside Rg₁ Response on K562 Human Leukemia Cell Line

Liu, Jun,Cai, Shi-Zhong,Zhou, Yue,Zhang, Xian-Ping,Liu, Dian-Feng,Jiang, Rong,Wang, Ya-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Aims and Background: Traditional chemotherapy strategies for human leukemia commonly use drugs based on cytotoxicity to eradicate cancer cells. One predicament is that substantial damage to normal tissues is likely to occur in the course of standard treatments. Obviously, it is urgent to explore therapies that can effectively eliminate malignant cells without affecting normal cells. Our previous studies indicated that ginsenoside $Rg_1$ ($Rg_1$), a major active pharmacological ingredient of ginseng, could delay normal hematopoietic stem cell senescence. However, whether $Rg_1$ can induce cancer cell senescence is still unclear. Methods: In the current study, human leukemia K562 cells were subjected to $Rg_1$ exposure. The optimal drug concentration and duration with K562 cells was obtained by MTT colorimetric test. Effects of $Rg_1$ on cell cycle were analyzed using flow cytometry and by SA-${\beta}$-Gal staining. Colony-forming ability was measured by colony-assay. Telomere lengths were assessed by Southern blotting and expression of senescence-associated proteins P21, P16 and RB by Western blotting. Ultrastructural morphology changes were observed by transmission electron microscopy. Results: K562 cells demonstrated a maximum proliferation inhibition rate with an $Rg_1$ concentration of $20{\mu}\;mol{\cdot}L^{-1}$ for 48h, the cells exhibiting dramatic morphological alterations including an enlarged and flat cellular morphology, larger mitochondria and increased number of lysosomes. Senescence associated-${\beta}$-galactosidase (SA-${\beta}$-Gal) activity was increased. K562 cells also had decreased ability for colony formation, and shortened telomere length as well as reduction of proliferating potential and arrestin $G_2$/M phase after $Rg_1$ interaction. The senescence associated proteins P21, P16 and RB were significantly up-regulated. Conclusion: Ginsenoside $Rg_1$ can induce a state of senescence in human leukemia K562 cells, which is associated with p21-Rb and p16-Rb pathways.

Whole Brain Radiotherapy Plus Chemotherapy in the Treatment of Brain Metastases from Lung Cancer: A Meta-analysis of 19 Randomized Controlled Trails

Liu, Wen-Jing,Zeng, Xian-Tao,Qin, Hai-Feng,Gao, Hong-Jun,Bi, Wei-Jing,Liu, Xiao-Qing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Objective: To evaluate the efficacy and safety of whole brain radiotherapy (WBRT) plus chemotherapy versus WBRT alone for treating brain metastases (BM) from lung cancer by performing a meta-analysis based on randomized controlled trials (RCTs). Methods: The PubMed, Embase, CENTRAL, ASCO, ESMO, CBM, CNKI, and VIP databases were searched for relevant RCTs performed between January 2000 and March 2012. After quality assessment and data extraction, the meta-analysis was performed using the RevMan 5.1 software, with funnel plot evaluation of publication bias. Results: 19 RCTs involving 1,343 patients were included. The meta-analyses demonstrated that compared to WBRT alone, WBRT plus chemotherapy was more effective with regard to the objective response rate (OR = 2.30, 95% CI = 1.79 - 2.98; P < 0.001); however, the incidences of gastrointestinal reactions (RR = 3.82, 95% CI = 2.33 - 6.28, P <0.001), bone marrow suppression (RR = 5.49, 95% CI = 3.65 - 8.25, P < 0.001), thrombocytopenia (RR = 5.83, 95% CI = 0.39 - 86.59; P = 0.20), leukopenia (RR = 3.13, 95% CI = 1.77 - 5.51; P < 0.001), and neutropenia (RR = 2.75, 95% CI = 1.61 - 4.68; P < 0.001) in patients treated with WBRT plus chemotherapy were higher than with WBRT alone. There was no obvious publication bias detected. Conclusion: WBRT plus chemotherapy can obviously improve total efficacy rate, butalso increases the incidence of adverse reactions compared to WBRT alone. From the limitations of this study, more large-scale, high-quality RCTs are suggested for further verification.

Effect of combination of chitosan coating and irradiation on physicochemical and nutritional properties of chicken egg during storage room temperature

Xian De Liu,Binna Kim,Youn Kook Jeong,Ju Woon Lee,CheorunJo 한국가금학회 2009 한국가금학회 정기총회 및 학술발표회 Vol.26 No.-

One-d-old fresh chicken egg was coated with 1% chitosan solution(pH 5.0) and irradiated at 0, 0.5, 1.0, 1.5 and 2.0 kGy by gamma ray. The egg samples were stored at room temperature for 14 days and the effects of the combination treatment on internal physicochemical and nutritional properties of the egg were investigated. Combination of chitosan coating and irradiation did not show any effect on proximate composition, water activity, and mineral contents. However, carotenoid content was decreased by irradiation with increasing irradiation dose. The cholesterol content of 2 kGy irradiation group was lower than that of non irradiation group. The total phospholipids content was not affected by chitosan coating and irradiation treatment.

Differential Proteome Analysis of Breast and Thigh Muscles between Korean Native Chickens and Commercial Broilers

Liu, Xian De,Jayasena, Dinesh D.,Jung, Yeon-Kuk,Jung, Samooel,Kang, Bo-Seok,Heo, Kang-Nyeong,Lee, Jun-Heon,Jo, Cheo-Run Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.6

The Korean native chickens (Woorimotdak$^{TM}$, KNC) and commercial broilers (Ross, CB) show obvious differences in meat flavor after cooking. To understand the contribution of protein and peptide for meat flavor, 2-dimensional (2-D) gel electrophoresis and matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry was performed. A total of 16 protein spots were differentially expressed in the breast and thigh meat between the two breeds. A total of seven protein spots were represented by different levels between KNC and CB for breast meat. Among them three protein spots (TU39149, TU40162 and TU39598) showed increases in their expressions in KNC while other four protein spots (BU40125, BU40119, BU40029 and BU39904) showed increases in CB. All nine protein spots that were represented by different levels between KNC and CB for thigh meat showed increases in their expression in KNC. Phosphoglucomutase 1 (PGM 1), myosin heavy chain (MyHC), heat shock protein B1 (HSP27), cytochrome c reductase (Enzyme Q), Glyoxylase 1, DNA methyltransferase 3B (DNA MTase 3) were identified as the main protein spots by MALDI-TOF mass spectrometry. These results can provide valuable basic information for understanding the molecular mechanism responsible for breed specific differences in meat quality, especially the meat flavour.

Prevalence and Related Factors of EPS among Schizophrenic Inpatients

Liu, Lian-Qi,Liu, Xian-Chen,Hu, Lei,Chen, Kun,Li, Chuan-Qi 용인정신의학연구소 1998 용인정신의학보 Vol.5 No.1

Combination of chitosan-coating and irradiation on nutritional and sensory quality of egg

Xian De Liu,Aera Jang,Dong Ho Kim,Cheorun Jo 한국가금학회 2007 한국가금학회 정기총회 및 학술발표회 Vol.24 No.-

An experiment was desi gned t o improve the safety of chicken egg by the combination of chitosan-coating and irradiation process. One-day old hen egg produced from Rohman Brown with 26-week old was collected and coated with 2% chitosan solution. The sample egg was then irradiated at 0 and 2.0 kGy by gamma ray to see the effect of combination of chitosan-coating and gamma irradiation on nutritional quality of egg. Nutritional quality characteristics of hen egg studied included proximate composition, amino acid profiles, water activity, and mineral content in egg yolk and white. Sensory evaluation of cooked egg with different treatments also conducted. Chitosan-coating of egg resulted in a little decrease of amino acid contents in both yolk and white but the effect of irradiation was not found. There was no change found in other nutritional quality characteristics including proximate composition, and mineral contents. Sensory score indicated that chitosan-coating, irradiation, and their combination was not changed. Therefore, it can be concluded that chitosan-coating, irradiation, and the combination of those may affect on the egg quality very limitedly.