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A report of 35 unreported bacterial species in Korea, belonging to the phylum Firmicutes
Baek, Min-gyung,Kim, Wonyong,Cha, Chang-Jun,Joh, Kiseong,Kim, Seung-Bum,Kim, Myung Kyum,Seong, Chi-Nam,Yi, Hana The National Institute of Biological Resources 2019 Journal of species research Vol.8 No.4
In an investigation of indigenous prokaryotic species in Korea, a total of 35 bacterial strains assigned to the phylum Firmicutes were isolated from diverse habitats including natural and artificial environments. Based on their high 16S rRNA gene sequence similarity (>98.7%) and formation of robust phylogenetic clades with species of validly published names, the isolates were identified as 35 species belonging to the orders Bacillales (the family Bacillaceae, Paenibacillaceae, Planococcaceae, and Staphylococcaceae) and Lactobacillales (Aerococcaceae, Enterococcaceae, Lactobacillaceae, Leuconostocaceae, and Streptococcaceae). Since these 35 species in Korean environments has not been reported in any official report, we identified them as unrecorded bacterial species and investigated them taxonomically. The newly found unrecorded species belong to 20 species in the order Bacillales and 15 species in the order Lactobacillales. The morphological, cultural, physiological, and biochemical properties of the isolates were examined and the descriptive information of the 35 previously unrecorded species is provided here.
A Metaviromic Analysis of Viral Communities in the Feces of Unexplained Acute Gastroenteritis
Baek, In Hyuk,Kim, Song Ah,Kim, Jong-Hwa,Park, Hee Kuk,Kim, Wonyong The Korean Society for Microbiology 2013 Journal of Bacteriology and Virology Vol.43 No.4
Although viruses are the most common cause of acute gastroenteritis (AGE) in humans, details about the causative viruses in AGE are largely unknown because many causative viruses are unable to be cultured by current culture techniques. In our study, fecal samples from 10 children under five years of age with unexplained AGE and 10 healthy children were investigated for RNA viruses using random priming (RP)-mediated sequence-independent single primer amplification (SISPA). The causative viruses in cases of cryptogenic diarrhea were then assessed for their potential diagnostic value. Of the 1,129 viral clones identified, rotavirus was most commonly associated with AGE (125 sequences, 22.4%). In contrast, bacteriophage was most common (43 sequences, 13.6%) in healthy children. The remaining 515 viral clones were unidentifiable. These findings suggest that investigation of cases or outbreaks of unexplained diarrhea using a metaviromic strategy is a new avenue for diagnosis.
Baek, In Hyuk,Lee, Hyun Woong,Kim, Hyung Joon,Song, Mi-Ok,Yoon, Seung-Kew,Park, Jong-Hwa,Chung, In Sik,Kim, Wonyong The Korean Society for Microbiology 2014 Journal of Bacteriology and Virology Vol.44 No.3
Hepatitis A virus (HAV) positive stool samples were collected from acute hepatitis A patients during the two study periods of 2002 and 2011 in Seoul, South Korea, and their genetic variability was determined. From a total of 79 specimens, the nucleotide sequences of the VP1 and 2A junction were successfully amplified in 27 (34.2%) samples and subjected to sequence analysis. Genetically, there was a dramatic change in HAV subgenotypes from IA to IIIA during the past ten years. Sequence analysis identified that most strains belonged to genotype I, which is the main genotype globally. The subgenotype IA (93.3%, n=13/14) was the major subgenotype in 2002, whereas the subgenotype IIIA (69.2%, n=9/13) was predominant in 2011. Interestingly, a IIIA strain was identified from a patient who had a history of travel to India in 2002. The finding presented provides new insight into the genetic shift of circulating HAVs in South Korea.
Baek Jihye,Kim Jong-Hwa,Kim Wonyong 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.6
Lactococcus lactis is a lactic acid bacterium and used in the dairy food industry. The ameliorating effects of Lactobacillus species on atopic dermatitis (AD) have been extensively studied, but the specific effect of L. lactis strains has not yet been investigated. In this study, the efficacy of L. lactis LB 1022, isolated from natural cheese, was evaluated using RAW 264.7, HMC-1 and HaCaT cell lines and an ovalbumin-sensitized AD mouse model. L. lactis LB 1022 exhibited nitric oxide suppression and anti-allergy and anti-inflammatory activity in vitro. Oral administration of L. lactis LB 1022 to AD mice significantly reduced the levels of IgE, mast cells, and eosinophils, and a range of T cell-mediated T helper Th1, Th2, and Th17-type cytokines under interleukin (IL)-10, transforming growth factor-β (TGF-β), thymus and activation-regulated chemokine (TARC), and thymic stromal lymphopoietin (TSLP). In addition, L. lactis LB 1022 treatment increased the concentration of short-chain fatty acids. Overall, L. lactis LB 1022 significantly modulated AD-like symptoms by altering metabolites and the immune response, illustrating its potential as candidate for use in functional food supplements to alleviate AD.
Transcription factor IRF8 controls Th1-like regulatory T-cell function
Lee, Wonyong,Kim, Hyeong Su,Baek, Song Yi,Lee, Gap Ryol CHINESE SOCIETY OF IMMUNOLOGY 2016 CELLULAR AND MOLECULAR IMMUNOLOGY Vol.13 No.6
<P>Recent studies have suggested that regulatory T (Treg) cells comprise a heterogeneous population that regulates various aspects of the immune response, and that Treg cells use the factors that are expressed in their target cells to regulate them. We searched for factors that regulate Th1 response in Treg cells using a meta-analysis. In the process, we discovered that transcription factor interferon regulatory factor 8 (IRF8) was selectively expressed in Treg and Th1 cells. IRF8-deficient Treg cells showed defective expression of CXCR3 and aberrant expression of the II4 and II17 genes. Upon treatment with alpha galactosyl-C18-ceramide (alpha Gal-C18-Cer), IRF8-deficient mice showed defective Treg cell recruitment in the liver. Eliciting Th1 immune response by anti-CD40 antibody injection in mice induced IRF8 expression in Treg cells. The expression of IRF8 was induced by Foxp3 in Treg cells. IRF8 had no effect on T-bet expression in Treg and vice versa. Thus, our results strongly suggest that IRF8 controls Th1 immune response in Treg cells independent of T-bet.</P>
일측 만성 중이질환 환자에서 비디오 두부충동검사를 이용한 전정안구반사의 평가: 예비연구
남기성,Wonyong Baek,조성일 대한평형의학회 2022 Research in Vestibular Science Vol.21 No.3
Objectives: This study aims to investigate the usefulness of the video head im-pulse test (vHIT) as a method for evaluating vestibular function in patients with unilateral chronic middle ear disease. Methods: Nineteen patients with various unilateral middle ear diseases including chronic otitis media (COM), COM with cholesteatoma, and adhesive otitis media successfully underwent preoperative vHIT. The gain of vestibuloocular reflex (VOR) and the presence of corrective saccades were compared between the affected ear and the healthy ear. Results: A total of eight male and 11 female patients with a mean age of 43.1 years were included in this retrospective study. Twelve ears had COM, four had COM with cholesteatoma, and three had adhesive otitis media. A positive history of vertigo or dizziness was reported in 36.8% of the cases. The VOR gain of the affected ears and the healthy ears were 0.97±0.16 vs. 1.00±0.08 in the horizontal canal, 0.91±0.11 vs. 0.87±0.11 in the anterior canal, and 0.87±0.17 vs. 0.99±0.12 in the posterior canal, respectively. Only VOR gain of the posterior canal was sig-nificantly decreased compared with healthy ears. Conclusions: The average VOR gains in the patients with chronic middle ear disease were within the pre-defined values of normality; however, when compared quantitatively, posterior canal gain of affected ears was significantly decreased com-pared to healthy ears. Our results can be explained by the anatomical proximity of the ampulla of the posterior canal and middle ear space.
( In Hyuk Baek ),( Hyung Joon Kim ),( Hyun Woong Lee ),( M Ok Song ),( Thoi Cong Truong ),( Seung Kew Yoon ),( Hee Young Lee ),( Dong Hwa Shon ),( In Sik Chung ),( Wonyong Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background: Recently, hepatitis A has become increasingly important because its incidence is dramatically increasing in adolescents and adults in South Korea. HAV infection is now recognized as a public health burden. This situation has resulted in the need for genetic and epidemiological evaluation of hepatitis A virus (HAV) infection. We performed a comparative HAV genetic analysis between non-endemic period and endemic period. Methods: HAV IgM-positive fecal samples were collected from patients with acute hepatitis A at two university hospitals in South Korea during the two study periods of 2002 (n=34) and 2011 (n=45) and their genetic variability was characterized. Results: From a total of 79 specimens, the nucleotide sequences of the VP1 and 2A regions were successfully amplified in 27 (34.2%) samples and subjected to sequence analysis. Phylogenetic analysis showed that most strains belonged to genotype I, which is the major genotype worldwide. The subgenotype IA (93.3%, 13/14) was predominant in 2002, whereas the subgenotype IIIA (69.2%, 9/13) was the major subgenotype in 2011. Notably, a IIIA strain was detected in 2002 from a patient who had a history of travel to India. Conclusions: Genetically, there was a dramatic shift in circulating HAV subgenotypes from IA to IIIA during the past ten years. This report provides new insight into the genetic variation and relatedness of HAV circulating in South Korea.
A report of 28 unrecorded bacterial species, phylum Bacteroidetes, in Korea
Maeng, Soohyun,Baek, Chaeyun,Bae, Jin-Woo,Cha, Chang-Jun,Jahng, Kwang-Yeop,Joh, Ki-seong,Kim, Wonyong,Seong, Chi Nam,Lee, Soon Dong,Cho, Jang-Cheon,Yi, Hana The National Institute of Biological Resources 2018 Journal of species research Vol.7 No.2
In order to investigate indigenous prokaryotic species diversity in Korea, various environmental samples from diverse ecosystems were examined. Isolated bacterial strains were identified based on 16S rRNA gene sequences, and those exhibiting at least 98.7% sequence similarity with known bacterial species, but not reported in Korea, were selected as unrecorded species. 28 unrecorded bacterial species belonging to the phylum Bacteroidetes were discovered from various habitats including wastewater, freshwater, freshwater sediment, wet land, reclaimed land, plant root, bird feces, seawater, sea sand, tidal flat sediment, a scallop, marine algae, and seaweed. The unrecorded species were assigned to 18 different genera in five families: Flavobacterium, Epilithonimonas, Dokdonia, Gillisia, Flavicella, Chryseobacterium, Algibacter, Aquimarina, Lacinutrix, Gaetbulibacter, Cellulophaga, Tenacibaculum, and Maribacter of Flavobacteriaceae, Dyadobacter of Cytophagaceae, Draconibacterium of Draconibacterium_f, Sunxiuqinia of Prolixibacteraceae, and Fulvivirga of Fulvivirga_f. The selected isolates were subjected to further taxonomic characterization including analysis of Gram reaction, cellular and colonial morphology, biochemical activities, and phylogenetic trees. Descriptive information of the 28 unrecorded species is provided.