Cisplatin에 의한 흰쥐 공장의 alkaline phosphatase 활성변화에 미치는 SOD의 영향

김성목,현성희,전영희,문원진,백두진 한양대학교 의과대학 1995 한양의대 학술지 Vol.15 No.1

Cisplatin has been widely used as an effective anticancer platinum compound. Cisplatin incorporates with intrastrand or interstrand of the DNA and it inhibits the synthesis of the nucleic acid and protein. During the treatment of cancer patients, cisplatin acts on normally proliferating cells, which results in nephrotoxicity, nausea, vomiting, anorexia, bone marrow suppression and inner ear damage. These toxicities are related to oxygen free radicals formation by cisplatin, which inhibits the activity of the catalase and admages the mitochondria. The author has demonstrated the effect of superoxide dismutase(SOD), one of the antioxidant enzymes, on changes of alkaline phosphatase activities in the jejunum of the cisplatin treated rats. A total of 60 healthy Sprague-Dawley rats weighing about 200gm were used as an experimental animals. Cisplatin(6mg/kg) was administered intraperitoneally with pretreatment of 15000unit/kg of SOD or not. The experimental animals are sacrificed at 6 hours, 12 hours, 24 hours and 3 days after the administration of cisplatin. A part of jejunum were obtained and fixed in 10%-neutral formalin at 4℃. The speciments were sectioned 16㎛ thickness in frozen cryostat. The activity of alkaline phosphatase was evaluated histochemically by Gomori's method and incubation time of sections of specimens was 30minutes. The results were as follows. 1. SOD increase the activity of alkaline phosphatase in the epithelium of jejunum of the rat. 2. The activities of alkaline phosphatase of the epithelium of the mucosa decrease in jejunum of 6 hours and 12 hours of cisplatin treated rats. In the 24 hours and 3 days, activities of alkaline phosphatase of the epithelium increase. The atrophied villi are observed in the 12 hours and 24 hours of cisplatin treated group. 3. The activities of alkaline phosphatase increase in 12 hours and 3 days of cisplatin treated group with SOD pretreatment, the activities decrease in 6 hours of cisplatin treated group with SOD pretreatment. The activities of alkaline phosphatase in the 24 hours of cisplatin treated group with SOD pretreatment show a decided decrease over those of control group with SOD pretreatment. The atrophied villi are seen in the 12 hours and 24 hours of cisplatin treated group with SOD pretreatment. 4. The activities of alkaline phosphatase in the cisplatin treated group with SOD pretreatment are higher than those in the cisplatin treated group, except 24 hours of cisplatin treated group. 5. There are very little changes of alkaline phosphatase activities in the lamina propria, submucosa and muscle layer of all groups. Consequently, these results suggests that cisplatin decreases the activity of the alkaline phosphatase and SOD pretreatment decreases the range of the change in alkaline phosphatase activity of jejunum of the cisplatin treated rats.

스플라인을 사용한 날염디자인제도

김준목,정원용 경남대학교 정보통신연구소 2000 연구논문집 Vol.2 No.-

기존의 수제도 및 날염 CAD 디자인제도에서의 가장 큰 문제점은 하나의 디자인에 대한 드로잉 시간 및 소요인원이 너무 많이 요구되고 있다는 것이다. 본 논문에서는 이러한 문제점을 해결하기 위해 디자인제도에 디지털 영상처리를 이용함으로서 작업시간 및 인력 절감의 효과를 얻고자 한다. 특히 컬러분할을 위해 원 이미지를 전처리 한 후, 컬러 분할을 수행하였다. 이렇게 분할된 이미지의 윤곽선은 일반적으로 윤곽선이 불분명하고 거칠어 스플라인 기법을 사용하여 부드럽고 완만한 곡선이 얻어지도록 하였다. 모든 과정은 Matlab GUI를 사용하여 구현하였으며 분할 처리된 결과 이미지는 실제의 날염제도공정에 적용할 수 있을 정도로 개선되었음을 확인하였다. Lots of time and person's labor are required to draw a design by hand printing in the fields of textile printing design tracing. One of the solution to attack the problem is to make use of the digital image processing method over a design. In order to extract colors the preprocessing stage is required to enhance the quality of the design. And then, spline interpolation method is applied to make line smoother and more evident helpful for the color segmentation. Matlab GUI is used for implementation of the textile printing design tracing software.

금속도금공장 폐수중의 구리 및 시안화물의 효과적인 처리방법

諸元穆,洪鍾斗,金冕燮 漢陽大學校 環境科學硏究所 1988 環境科學論文集 Vol.9 No.-

구리 및 시안화물이 혼합된 도금폐수를 적절하게 처리하는 방법을 알기 위하여 pH,농도 및 접촉시간에 따르는 용액중 구리 및 시안화물의 제거율을 실험하였다. 그 결과 시안화물은 5% NaOC1용액으로 처리하면 잔류시안 농도가 0.03 ㎎/ℓ이하로 환경배출기준에 적합하였다. pH에 따르는 영향은 pH8 이상에서 99%제거율을 나타냈다. 구리혼합용액은 중화침전법으로 실험한 결과 pH8에서 99%가 짧은 시간에 제거되었다. 시안화물과 구리혼합용액의 pH에 따르는 제거율은 pH8 및 11에서 높은 제거율(95%이상)을 보였으며 pH10에서 제거율이 떨어지는 결과를 얻었다. To investigate the effective removal method of copper and cyanide compounds in metal plating waste water, removal ratio of copper and cyanide compounds in solution are measured with varying pH, concentration and contact time. As results of the present expriment, cyanide compounds in the solution are removed to 0.03 ㎎/ℓ or less with 5% NaOC1 solution. The present result is satisfied to environmental disposal standards. The removal ratio against pH values show 99% over pH8. As results of neutral precipitation method, copper including solution are removed to 99% at pH8 in short time. The removal ratios of cyanide mixed copper solution against pH values show high efficiency(over 95%) at pH8 and 11 and removal ratios are decreased at pH10.

디지털 설계를 위한 작은 크기와 4bit(LSB) 오차를 가진 10비트 감마(γ)시스템 설계

장원우,김현식,이성목,김인규,강봉순 東亞大學校附設 淸報技術硏究所 2006 情報技術硏究所論文誌 Vol.13 No.2

이 논문에서, 제시된 감마(r)라인 시스템은 해당 공식에 의해 만들어진 비선형 감마 곡선과 하드웨어로 구현된 결과 사이의 오차를 최소화하기 위해 만들어 졌다. 제시된 알고리즘과 시스템은 특정 감마값이 2.2, 즉 {0,1}^(2.2)에 의해 생성되는 공식과 입, 출력 데이터 크기가 10bit를 기반으로 한다. 오차를 최소화하기 위해, 시스템은 데이터 점들 사이를 지나 적합한 다항식을 만드는 수치해석 방법, 최소 자승 다항식을 사용하였다. 제한된 감마 라인은, 정밀도를 높이기 위해, 서로 각각의 중첩된 범위를 가지는 2차 다항식 9개로 구성되어 있다. MATLAB^(TM)7.0으로 검증된 알고리즘을 바탕으로, 제한된 시스템은 Verilog-HDL으로 구현되었다. 시스템은 2 클럭 지연을 가지며 1 클럭마다 결과가 생성된다. 오차 범위 (LSB)는 -4에서 +3이다. 표준편차는 1.287956238을 가진다. 시스템의 전체 게이트 값은 2,083이며, 최대 타이밍은 15.56[ns] 이다.

回轉圓板에 依한 窒酸化에 關한 硏究

諸元穆,鄭渭得,金冕燮 漢陽大學校 環境科學硏究所 1989 環境科學論文集 Vol.10 No.-

回轉圓板을 利用하여 COD除去와 室酸化를 同時에 效率的으로 達成하기 위하여 일련의 實驗을 行하였다. 回轉圓板에서 박리시킨 Sludge를 현탁 狀態에서 利用하여 室酸化에 미치는 pH의 影響을 檢討하였으며, 最適 pH값은 7.5∼8.5이었다. 많은 量의 酸素供給에 依해서도 室酸化는 增加되지 않았으며, 그 까닭은 놓은 COD負荷條件下에서 COD를 利用하는 微生物君에 依해 두꺼워진 生物膜이 室酸化에 必要한 酸素와 基質移動을 制限한 때문인 것으로 推定되었다. 回轉圓板을 利用한 室酸化를 爲해서는 COD負荷는 0.027㎎/㎝²·hr以下로 制限되어야 하고, 또 높은 COD와 無機室素를 合有하는 廢水의 處理에는 多段 System이 適用되어야 한다는 것을 알 수 있었다. Rotating biological disc contactor system was used in order to accomplish the COD removal and nitrification simultaneously in one step. The effect of pH on nitrification rate was examined in a suspended growth system, using microorganism scraped from the rotating discs, and an optimum pH value was between 7.5 and 8.5. Nitrification was not accompanied by a large amount of dissolved oxygen supplied in the system. This might be attributed to the inreased thickness of the COD-utilizer-biofilm limited oxygen and substrate transfer for nitrification under high COD loading. It was found that the COD loading must be reduced to below 0.027㎎/㎝²·hr, for nitrification to occur in the rotating disc system, and that a multi-stage system could improve the treatment of waste water containing both high COD and inorganic nitrogen.

Low-cost Front-end Multi-standard video encoder의 설계

이성목,김현식,장원우,김인규,강봉순 東亞大學校附設 淸報技術硏究所 2006 情報技術硏究所論文誌 Vol.13 No.2

본 논문은 Low-cast Front-end Multi-standard video encoder의 설계와 시뮬레이션에 관한 것이다. 비디오 인코더는 ITU-R(International Telecommunication Union-recommendation)에서 제시한 BT.601 4:2:2 비디오 포맷을 비롯한, ITU-R BT 656 4:2:2 비디오 포맷 등 다양한 영상신호를 전 세계의 컬러 TV 방송 표준인 NTSC, PAL, SECAM 등의 CVBS 표준 규격에 맞게 변환하는데 목적이 있겠다. 하드웨어를 설계할 때 중요한 고려사항으로는 하드웨어의 크기, 동작 속도(연산 속도), 연산의 정확도 등을 들 수 있는데, 본 논문에서는 이들 중 하드웨어의 크기에 가장 큰 중점을 두고 비디오 인코더를 설계하였다. 하드웨어의 크기가 커지면 제작 비용이 증가하고 또한 연산속도의 저하를 가져오게 된다. 그래서 우리는 하드웨어 사이즈를 줄이고 이에 비해 사용자는 정보의 손실을 느끼지 못하도록 MATLAB을 이용한 시뮬레이션을 수행하였다. 그 결과를 기준으로 적절한 데이터 패스의 비트수를 결정하였다.

加減茵蔯蒿湯 水鍼液이 膽道結紮로 유도된 肝損傷에 미치는 영향

成樂箕,金元信,田炳薰,李建穆 대한동의병리학회 1993 동의생리병리학회지 Vol.8 No.-

연구배경 : 肝癌 및 肝硬變은 韓國人의 疾病으로 인한 중요한 喪亡原因의 하나이며, 특히 우리나라 B型肝炎의 流行地域으로 急慢性 肝炎이나 肝經辨證, 肝癌의 發病率이 높다. 그리하여 肝硬變症을 寢具治療法중 水鍼을 이용하여 肝의 募穴인 期門穴에 投與하면 治療效果가 있는지에 대하여, 그리고 藥量에 따라 治療效果가 달라지는 지에 대하여 알아보기 위하여 본 연구를 시행하였다. 방법 : 加減茵蔯蒿湯 水鍼液을 膽道를 結紮한 白鼠에 26guage의 1ml 주사기를 이용하여 투여하였다. 經穴은 人體의 期門에 相應하는 左石部位와 腹部內의 任意의 部位를 設定하여 매일 1회 7주간 수침하였다. 결과 : 體重과 Total Bilirubin은 實驗群 A, C에서 有意性 있는 增加와 減少를 보였고, WBC·ALT·AST·ALT 및 Cholesterole은 全實驗群에서 有意性 있는 減少를, Albumin은 全實驗群에서 有意性 있는 增加를 각각 나타내었으며, 組織學的 觀察도 全實驗群에서 有意性을 나타내었다. 또한 Hydroxyproline도 實驗群 A, B에서 有意性있는 減少를 보였다. 결론 : 加減茵蔯蒿湯 水鍼液을 膽道를 結紮한 白鼠의 期門穴에 投與한 缺課 有意한 효과를 보였으나 水鍼量에 따라 有意性의 차이가 있어 敵意한 水鍼量을 투여하는 것이 治療시 重要하다고 思料되며, 또한 水鍼에 대한 安全性 問題에 대하여서도 더욱 硏究할 필요가 있다. In order to study the effects of the water extract of Gagaminjinhotang in the Meridian points Liv 14 on the liver damage indued by bile duct ligation during 7 weeks after the operation of bile duct ligation, blood was taken from the heart and body weight, WBC, RBC, hemoglobin, hematocrit, total bilirubin, ALT, AST, ALP, cholesterol, albumin and hydroxyproline were in each experimental apparatus. The results of this study were as follows; 1. Body weight of EXA, C were significantly increased by the water extract of Gagaminjinhotang. 2. WBC of EXA,B,C were significantly decreased by the water extract of Gagaminjinhotang. 3. RBC, hemoglobin and hematocrit of EXA,B,C were not significantly influenced by the water extract of Gagaminjinhotang. 4. Total bilirubin of EXA,C and direct bilirubin of EXA,B,C were significantly decreased by the water extract of Gagaminjinhotang. 5. ALT, AST,ALP of EXA,B,C were significantly decreased by the water extract of Gagaminjinhotang. 6. Cholesterol of EXA,B,C, were significantly decreased by the water extract of Gagaminjinhotang. 7. Albumin of EXA,B,C, were significantly increased by the water extract Gagaminjinhotang. 8. Hydroxyproline of EXA,B were significantly decreased by the water extract Gagaminjinhtang. 9. On the histological observation, the results of the experimental groups showed significant hepatoprotective effects compared with control group from 3 weeks after the administration of the water extract of Gagaminjinhotang. According to the above result, it is suggested that the water extract Gagaminjinhotang was significantly effective in WBC, bilirubin, ALT, AST, ALP, cholesterol, albumin and hydroxyproline level and then it seems to be applicable to the fibrosis or cirrhosis of liver.

Characterizing Organelles in Live Stem Cells Using Label-Free Optical Diffraction Tomography

Kim, Youngkyu,Kim, Tae-Keun,Shin, Yeonhee,Tak, Eunyoung,Song, Gi-Won,Oh, Yeon-Mok,Kim, Jun Ki,Pack, Chan-Gi Korean Society for Molecular and Cellular Biology 2021 Molecules and cells Vol.44 No.11

Label-free optical diffraction tomography (ODT), an imaging technology that does not require fluorescent labeling or other pre-processing, can overcome the limitations of conventional cell imaging technologies, such as fluorescence and electron microscopy. In this study, we used ODT to characterize the cellular organelles of three different stem cells-namely, human liver derived stem cell, human umbilical cord matrix derived mesenchymal stem cell, and human induced pluripotent stem cell-based on their refractive index and volume of organelles. The physical property of each stem cell was compared with that of fibroblast. Based on our findings, the characteristic physical properties of specific stem cells can be quantitatively distinguished based on their refractive index and volume of cellular organelles. Altogether, the method employed herein could aid in the distinction of living stem cells from normal cells without the use of fluorescence or specific biomarkers.

Convexity Meningioma En Plaque Presenting with Diffuse Hyperostosis of the Skull

Kim, Sang-Mok,Jang, Kyung-Sool,Kim, Moon-Chan,Joo, Won-Il The Korean Neurosurgical Society 2006 Journal of Korean neurosurgical society Vol.39 No.2

Although meningioma is a common and benign intracranial tumor, meningioma en plaque is a rare tumor, especially in the cranial vault. Meningioma en plaque[MEP] usually occurs in the area of the sphenoid wing, and it causes cosmetic and visual problems, as well as the problems that are due to its mass effect. The authors present here a case of convexity meningioma en plaque that involved the skull and scalp with diffuse hyperostosis as the presenting salient radiological findings, which caused marked intraoperative bleeding.

CT scanning-based phenotypes vary with <i>ADRB2</i> polymorphisms in chronic obstructive pulmonary disease

Kim, Woo Jin,Oh, Yeon-Mok,Sung, Joohon,Lee, Young Kyung,Seo, Joon Beom,Kim, NamKug,Kim, Tae-Hyung,Huh, Jin Won,Lee, Ji-Hyun,Kim, Eun-Kyung,Lee, Jin Hwa,Lee, Sang-Min,Lee, Sangyeub,Lim, Seong Yong,Shin Elsevier 2009 Respiratory medicine Vol.103 No.1

<P><B>Summary</B></P><P><B>Background</B></P><P>Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease that is characterized by varying degrees of involvement of airway and lung parenchyma. Although cigarette smoke is the major risk factor for COPD, the principal determining factors of involvement of the airway or lung parenchyma have not been clearly defined. Genetic variability in COPD patients might influence the varying degrees of involvement of airway and parenchyma. We therefore studied whether airway and parenchyma involvement might be associated with the <I>ADRB2</I> genotype, which has been reported to be associated with COPD susceptibility and the bronchodilator response.</P><P><B>Methods</B></P><P>One hundred and eleven COPD subjects, whose post-bronchodilator FEV<SUB>1</SUB>/FVC values were less than 0.7, and who had histories of smoking exceeding 10 pack-years, were prospectively recruited from pulmonology clinics of 11 hospitals in Seoul, Korea. The degrees of involvement of airway and parenchyma were evaluated by volumetric computed tomography (CT) scans. In-house software automatically calculated luminal areas, airway wall areas, percentages of wall areas in segmental bronchi, emphysema indices, and mean lung densities in the whole lung parenchyma. The <I>ADRB2</I> genotypes at codon 16 were determined for all patients.</P><P><B>Results</B></P><P>Gly16 was associated with lumen diameter, luminal area, and percentage of wall area in patients with COPD (<I>p</I>=0.02), whereas neither wall area nor wall thickness differed with <I>ADRB2</I> genotype. Neither emphysema index nor mean lung density was associated with <I>ADRB2</I> genotype.</P><P><B>Conclusion</B></P><P>Gly16 variant in <I>ADRB2</I> gene was associated with airway wall phenotypes measured using CT scanning in COPD patients.</P>