중년여성의 약물사용 실태 조사

이영선,김은경,김경숙,강경인,김희선,신성희,김은숙,최지선,신혜숙,황선기 여성건강간호학회 2001 여성건강간호학회지 Vol.7 No.4

The purpose of this study was to investigate the drug use of middle aged women. The subject consisted of 330 middle aged women who ranged in age from 40 to 60 years. They were selected in Seoul, Kyung-Ki province, Korea. Data were collected by using questionnaires, from April, 10th to 30th 2001 and analyzed by the SPSS PC+ program using qui-square. The results are summarized as follows ; 1. The proportion of drinking and smoking experience was 42.4%, 3.7%. The motivation of drug use was advised family and relatives(46.1%), doctor's order(39.4%), magazine and advertisement(14.5%). Most of middle aged women get the information on knowledge of drug from hospital(47.3%), magazines and advertisement(30.3%), advised family and relatives(22.4%). And 57.9% of the middle aged women didn't know side effect of the using drug and 13.9% of the middle aged women have had experienced with side effect. And the level of attitude on drug abuse in middle aged women was 43%. Most of the subjects(93.9%) didn't use alternative drugs, and they used more than 3 kinds of drugs(47%). 2. They used digestives(44.2%), applying ointments(41.8%), drinks(39.4%), analgesics(39.1%), laxatives(8.8%), anti hypertension drugs (8.8%), and anti-anemic drugs(8.8%) in their orders.

Thermostable Alkalophilic Protease 생산균주의 효소학적 특성 및 분류동정

황경숙,이종화 목원대학교 자연과학연구소 2002 自然科學 硏究論文集 Vol.11 No.2

충남 홍성군 광천지역의 사문암 토양은 pH 9.2 이상을 나타내어 초염기성 토양임이 확인되었다. 알칼리성 세균 분리에 사용되는 alkaline 배지(AL 배지)를 이용하여 초염기성 사문암토양으로부터 알칼리성 세균 76균주를 분리하였다. 분리균주 중 49균주가 알칼리성단백질 분해효소 생산균주로 약 65%의 높은 분포율을 나타내었다. 이들 세균 중 알칼리성 단백질 분해효소의 활성이 가장 큰 KSIA-9균주를 선발하여 효소학적 특성을 검토한 결과, 분리균주 KSIA-9는 최적 온도가 60℃이며 70℃에서도 최대 활성의 90% 이상을 유지하여 열에대해 안정한 특징을 나타내었다. 그람양성, 유포자세균으로 165 rDNA 염기서열 분석결과 Bacillus thuringinesis와 99%의 상동성을 나타내어 Bacillus thuringinesis로 동정되었다. Seventy-six alkalophilic bactena were isolated from serpentinite soil(>pH9.2). About 65% of the isolates among them was found to produce alkalophilic protease. Especially because KS1A-9 strain among them appeared to be finally selected. The apparent characteristics of these isolates based on the phenotypic, chemotaxonomic, and phylogenetic analysis are as follows. KS1A-9 which produced alkalophilic and thermostable protease was gram-positive, sporeforming, regular rods. Respiratory quinone is MK-7 and MK-8, and major cellular fatty acids are iso-C_15^-0, iso-C_14 0 and anteiso-C_13^-0. When determining the relationship of this strain was closely (99%) related to Bacillus thuringiensis.

離農民의 都市生活 적응에 대한 硏究

黃淑慶 梨花女子大學校 師範大學 社會生活科 1982 綠友硏究論集 Vol.- No.24

지하수(음용수)내 미생물 의 정량적 평가

김인기,황경숙 목원대학교 자연과학연구소 2001 自然科學 硏究論文集 Vol.10 No.2

시판중인 광천수(CW, CJ, GR), 대평리, 초정리의 광천수(DPG, CJG1M CJG2), 도시인근 지역의 지하수 (TJ, WS), 폐광산 인근의 지하수 (KGW1, KGW2, KGW3)와 양돈농장 인근의 지하수 (NPG1, NPG2)로부터 채수한 지하수 시료 중의 세균수를 직접 생균수 측정법(direct viable count ; DVC)과 평판계수법 (plate count ; PC)을 이용하여 정량적으로 측정한 결과 전균수(total direct count; TDC)에 대한 DVC 생균수는 약55∼80%, 평판계수법(PC)에 의한 결과는 DVC 값의 1∼30%로 나타났다. 이는 지하수내에 생존해 있지만 배양이 불가능한 (viable but non-culturable: VBNC)세균이 높은 비율로 존재함이라고 판단된다. 한편, 다양한 농도의 영양배지 (NB, DNB, R2A, DR3A)를 이용하여 세균수를 계수한 결과, 통상농도의 NB배지에 비해 저영양배지인 DNB, R2A, DR3A에서 2∼50배 이상 높은 계수치를 나타내었다. 이와 같은 결과로부터 지하수와 같이 빈영양한 환경 내에는 통상 농도의 NB배지에서는 증식이 현저히 저해되고 저영양배지에서만 증식 가능한 저영양세균이 다수 분포하고 있음이라 판단된다. The direct viable count(DVC) method and plate count(PC) method of determining the number of living bacteria in potable groundwater were applied to samples collected from areas around Daejeon-Chungcheong. Each samples were enumerated by plate counting (PC) and direct viable counting (DVC) methods. The DVC comprised 55-80% of the total direct count(TDC) in all 13 samples. PC values were roughly 1-30% of DVC. Therfore, distribution of the viable but non-culturable (VBNC) bacteria in groundwater was thought that exist highly proportion. PC methods were used different four media as nutrient distance, and counting values were showed that 2-50 times higher on low nutrient agar media(dilution media ; DNB, R2A, and DR3A) conpare with a conventional NB medium. This results were indicated that oligotrophic (low-nutrient demanding) bacterial groups exist abundantly in groundwater.

지하수(음용수)로부터 분리된 저영양세균의 계통분류학적 특성

김인기,황경숙 목원대학교 자연과학연구소 2001 自然科學 硏究論文集 Vol.10 No.2

다양한 농도의 영양배지 (NB, DNB, R2A, DR3A)를 이용하여 세균수를 계수한 결과, 통상농도의 NB배지에 비해 저영양배지인 DNB, R2A, DR3A에서 2∼50배 이상 높은 계수치를 나타내었다. 이와 같은 결과로부터 지하수중에는 NB배지에서는 증식이 현저히 저해되고 DNB배지에서만 증식이 가능한 저영양세균(oligotrophic bacteria)이 다수 분포하고 있음이라 판단되어 DNB배지로부터 420균주의 저영양세균을 분리하였다. 본 연구에서는 광천수(CW, CJ, DPG, CJG1)에서 분리된 저영양세균 105 균주의 16S rDNA 염기서열을 결정하여 계통분류학적 특성을 검토한 결과 네 개의 주요한 그룹으로 분류되었다. Proteobacteria α-subdivision (49균주), β-subdivision(51균주), γ-subdivision(4균주)과 High G+C 방선균 (1균주)이었다. Proteobacteria α-subdivision에는 Afipia속, Blastobacter 속, Bradyrhizobium속, Caulobacter 속, Phenylobacterium 속, Rhizobium 속, Sphingomonas 속이 포함되었으며, β-subdivision에는 Acidovorax 속, Azonexus 속, Ferribacterium 속, Janthinobacterium 속, Leptothrix 속, Polaromonas 속, Variovorax 속, Zoogloea 속이 포함되어 있었다. γ-subdivision에는 Methylobacterium 속과 Rhodanobacter 속이 포함되었고, High G+C균주는 Nocardia속에 속하는 것으로 나타났다. Previously, we determined the number of PC, DVC and TDC in potable groundwater [1]. Total 756 strains were isolated from diluted nutrient broth(DNB) medium, many isolates did not show appreciable growth on NB medium. Such isolates whose growth was suppressed in full strength NB medium but not in DNB medium were called "DNB­organisms(oligotrophic bacteria)". Percentages of DNB-organisms among isolates were 64-76% with marketing mineral water(CW, CJ, GR sites), 50-98% with mineral groundwater(DPG, CJG1, CJG2 sites), 23-52% with polluted site (TJ, WS, NPG1, NPG2 sites). One-hundred two strains were isolated for oligotrophic bacteria and their taxonomic characteristics determined by phylogenetic analysis. Based on a phylogenetic analysis using 16S rDNA sequences, these isolates were found to fall within three major phylogenetic groups: proteobacteria belonging to the α(49 strains), β(50 strains), and γ(3 strains) subdivisions. Three major clusters were confirmed in proteobacteria α-subdivision.Sphingomonas, Caulobacter, and BANA(the first letters of Bradyrhizobium, Agromonas, Nitrobacter, and Afipia) clusters. Proteobacteria γ and β subdivisions were almost identical to that of unidentified proteobacteria-related isolates(similarity values, 93-96%). These results prove that the high microbial deversity of groundwater at genus and species levels.

Micromonospora sp. SA-246 균주가 생산하는 Isochromanequinone계 항생물질

여운형,윤봉식,황경숙,이정옥,유승헌 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

저영양성 미생물을 선택적으로 분리, 배양하여 새로운 생리활성물질을 탐색하던 중 SA-246 균주가 강한 항균활성 및 암세포주에 대한 세포독성을 나타내는 것을 발견하였다. SA-246 균주의 동정을 위하여 배양적, 형태적, 생리적 특성을 ISP 방법에 따라 조사한 결과 Micromonospora 屬에 속하는 것으로 동정하였으며 따라서 Micromonospora sp. SA-246으로 명명하였다. 배양액으로 부터 용매추출, silica gel column chromatography, preparative TLC, HPLC 등에 의하여 활성물질을 분리, 정제하였으며 UV 흡수, mass, NMR spectrum의 분석 결과 본 활성물질을 crisamicin A로 동정하였다. 화합물 SA-246은 그람 양성 세균에 항균활성을, 폐암세포주(A549), 난포암세포주(SK-OV-3), 피부암세포주(SK-MEL-2), 신경암세포주(XF498), 대장암세포주(HCT15)에 세포독성을 나타내었다. In the course of screening for new bioactive compounds from oligotrophs in soil, a microorganism, designated as SA-246 and now identified as Micromonospora sp., has been shown to produce a strong anitbacterial compound. The active compound was purified from broth filtrate by ethylacetate extraction, silica gel column chromatography, preparative TLC and HPLC, and was identified as crisamicin A based on mass and NMR spectral data. The compound SA-246 exhibited not only strong anitbacterial activity against Gram-positive bacteria but also cytotoxicity against cancer cell lines such as A549 (lung), SK-OV-3 (ovarian), SK-MEL-2 (melanoma), XF498 (central nervous system) and HCT15 (colon).

Micromonospora sp. SA-246 균주가 생산하는 Isochromanequinone계 항생물질

여운형,윤봉식,황경숙,이정욱,유승헌 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

저영양성 미생물을 선택적으로 분리, 배양하여 새로운 생리활성물질을 탐색하던 중 SA-246 균주가 강한 항균활성 및 암세포주에 대한 세포독성을 나타내는 것을 발견하였다. SA-246 균주의 동정을 위하여 배양적, 형태적, 생리적 특성을 ISP 방법에 따라 조사한 결과 Micromonospora 層에 속하는 것으로 동정하였으며 따라서 Micromonospora sp. SA-246으로 명명하였다. 배양액으로부터 용매추출, silica gel column chromatography, preparative TLC, HPLC등에 의하여 활성물질을 분리, 정제하였으며 UV 흡수, mass, NMR spectrum의 분석 결과 본 활성물질을 crisamicin A로 동정하였다. 화합물 SA-246은 그람 양성 세균에 항균활성을, 폐암세포주(A549), 난소암세포주(SK-OV-3), 피부암세포주(SK-MEL-2), 신경암세포주(XF498), 대장암세포주(HCT15)에 세포독성을 나타내었다. In the course of screening for new bioactive compounds from oligotrophs in soil, a microorganism, designated as SA-246 and now identified as Micromonospora sp., has been shown to produce a strong antibacterial compound. The active compound was purified form broth filtrate by ethylacetate extraction, silica gel column chromatography, preparative TLC and HPLC, and was identified as crisamicin A based on mass and NMR spectral data. The compound SA-246 exhibited not only strong antibacterial activity against Gram-positive bacteria but also cytotoicity against cancer cell lines such as A549 (lung), SK-OV-3 (ovarian), SK-MEL-2 (melanoma), XF498 (central nervous system) and HCT15 (colon).

연산동에서 집단발생한 장티브스환자에 대한 세균학적 관찰

李潤日,金慶淑,金明淑,黃基銑,李鍾訓 中央醫學社 1973 中央醫學 Vol.25 No.3

An attempt was made to isolate the causative agent from a group of febrile patients in a construction camp, where the endemic was occurred in a fashion of explosive, and it seemed to be a salmonellosis on the clinical symptoms. Direct rectal swabs and' food samples were taken repeatedly from febrile patients, and only the rectal swabs from the healthy persons to obtain the bacteriological specimens. Sera were also obtained from both healthy persons and febrile patients. Water of glen which penetrates through the camp could be worthy of notice as an infectious source, because one chronic typhoid carrier was found in a slum which was located on a steep ascent just outside of the camp. Typhoid bacilli was isolated from the specimens of 17 patients (58.8%) out of 29 febrile patients, and from 5 out of 92 healthy persons. Widal agglutinin rise was so delayed that it was found not so worthy of examination in the situation of the early management of the patients for prevention of the endemic.

Changes of Bacterial Population in a Forest Soil and Grouping of Oligotrophic Bacteria by Using Cellular Fatty Acid

Whang,Kyung Sook 牧園大學校 1997 論文集 - 牧園大學校 Vol.31 No.-

Oligotrophic bacteria were shown to exist abundantly in all layers of a forest soil at different layers(L. F. H and A layers) throughout the year. Analysis of the cellular fatty acid composition is useful for the classification and identification of various bacterial genera. It is considered that cellular fatty acid analysis is sutable for differentiation and grouping of bacterial isolates. The cellular fatty acids of 39 soil oligotrophs were analyzed. All strains were grouped into 6 clusters according to their fatty acid composition at the 85% similarity level, using average linkage cluster analysis and similarity values based on correlation coefficients. The 2- and 3- hydroxy fatty acids were found to be useful in grouping strains into five major groups, several of which were further differentiated into subgroups. Within each group or subgroup, qualitative and quantitative differences in profiles occurred for most strains.

축산폐기물의 퇴비화 과정중 미생물상의 변동

黃敬淑(Kyung-Sook Whang),張基運(Ki-Woon Chang) 한국토양비료학회 1996 한국토양비료학회지 Vol.29 No.3