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RISS 인기검색어
- 검색키워드
- 저자 : Wenqian Shang (검색결과 3 건)
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Dandan Zhang,Zheng Wang,Liyun Shi,Wenqian Shang,Zhenzhu Fu,Dan He,Song Lin He 한국화훼학회 2016 화훼연구 Vol.24 No.2
In the present study, the plantlets in vitro of Paeonia suffruticosa ‘Wu Long Peng Sheng’ were used as laboratory materials. The proteome during adventitious root induction process was investigated to sift the related proteins by two-dimensional electrophoresis and mass spectrometry. The results indicated that the protein spots were concentrated in the acidity gel region (pH 4 - 7) and the spots number had a dynamic change ranged from 373 to 462 at the process of root induction (0 – 7 d). 8 spots significantly changed were analyzed with a mass spectrometer and identified using associated software and databases. The peptide information of the 8 spots was similar to the ATP synthase β-subunit of P. suffruticosa (Spots 1 - 4 and 8), P. tenuifolia (Spots 5), P. californica (Spot 6) and P. brownie ( Spots 7) r espectiv ely. T he expression levels of protein spots 1, 4, 5, 6 and 7 was dramatically downregulated, and that of protein spots 2 and 3 had a slightly opposite tendency on the 3rd day. The obviously decreased period is particularly interesting as it was consistent with the induction period of adventitious root primordial of tree peony plantlet in vitro. The ATP synthase β-subunit could be consumed for assembling the ATP synthase in order to supply energy to the rooting process. Therefore, we speculated that the ATP synthase β-subunit was involved in adventitious root initiation of tree peony plantlets in vitro and we expect that further studies should be carried out in order to export its action mechanism.
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Zheng Wang,Yinglong Song,Liyun Shi,Yuzhen Guo,Wenqian Shang,Dan He,Song Lin He 한국화훼학회 2016 화훼연구 Vol.24 No.2
Carbon source, an essential nutrient for plant growth, mainly includes exogenous sugar and CO2 of the environment in vitro. Therefore, the exogenous sugar and CO2 of the environment make the important roles in tissue culture. The aim of this study is to investigate the effects of different sugar concentrations (0, 10, 15 and 30 g·L-1) on the growth of colored Zantedeschia in vitro under certain CO2 concentration and explore the optimal sugar concentration. The plantlets in vitro of colored Zantedeschia had the largest root number, root weight, and root vigor under 0 g·L-1 (sugar-free culture) treatment. And they had the largest plant height, leaf length and leaf chlorophyll content, but p oor r oot v igor under 3 0 g·L-1 sugar. This study indicated that the optimal condition for proliferation and seedling culture of colored Zantedeschia plantlets in vitro was MS medium with 30 g·L-1 sugar, and the suitable medium for rooting culture and transplanting of colored Zantedeschia was MS medium with sugar-free culture under CO2 enrichment condition.
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Zheng Wang,Gege Su,Songlin He,Liyun Shi,Dan He,Wenqian Shang,Dajuan Yang 한국원예학회 2021 원예과학기술지 Vol.39 No.1
Root pruning during early root development can optimize the root structure of woody plants. To determine the effects of root pruning on tree peony (Paeonia suffruticosa), seedlings of the tree peony cultivar ‘Fengdanbai’ were subjected to varying degrees or different styles of root pruning. Various indexes were analyzed, including the anatomical structure of the root, root vitality, the activity levels of antioxidant enzymes, and the contents of endogenous hormones and their ratios. Comprehensive morphological observation showed that pruning freshly germinated roots that are 3 cm long by 1/3 (i.e., removing 1 cm) yielded the greatest rooting rate (90%), lateral root initiation (2.34 per seedling), and subsequent growth. Analysis of physiological and biochemical indicators during the critical rooting period (3-5 days after root pruning) showed that the levels of superoxide dismutase and abscisic acid (ABA) were decreased, while the oxidase activities of peroxidase, polyphenol oxidase, and indole acetate oxidase and the contents of the endogenous hormones indole acetic acid (IAA), gibberellin (GA3), and zeatin (ZT) were increased. IAA plays a key role in the process of adventitious root formation and the ratios of IAA/GA3, IAA/ABA, and IAA/ZT reflect endogenous hormone homeostasis during adventitious root formation. IAA/ABA and IAA/ZT showed higher values in the pruned roots compared to the control roots, which favor adventitious root induction and elongation. These results provide a basis for the study of adventitious root formation in tree peony.
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