RISS 학술연구정보서비스

다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기


      오늘 본 자료

      • 오늘 본 자료가 없습니다.
      • 무료
      • 기관 내 무료
      • 유료
      • KCI등재

        Protein Associated with Adventitious Root Induction Analysis of Tree Peony (Paeonia suffruticosa Andr.) Plantlets In Vitro by Two-dimensional Electrophoresis and Mass Spectrometry

        Dandan Zhang,Zheng Wang,Liyun Shi,Wenqian Shang,Zhenzhu Fu,Dan He,Song Lin He 한국화훼학회 2016 화훼연구 Vol.24 No.2

        In the present study, the plantlets in vitro of Paeonia suffruticosa ‘Wu Long Peng Sheng’ were used as laboratory materials. The proteome during adventitious root induction process was investigated to sift the related proteins by two-dimensional electrophoresis and mass spectrometry. The results indicated that the protein spots were concentrated in the acidity gel region (pH 4 - 7) and the spots number had a dynamic change ranged from 373 to 462 at the process of root induction (0 – 7 d). 8 spots significantly changed were analyzed with a mass spectrometer and identified using associated software and databases. The peptide information of the 8 spots was similar to the ATP synthase β-subunit of P. suffruticosa (Spots 1 - 4 and 8), P. tenuifolia (Spots 5), P. californica (Spot 6) and P. brownie ( Spots 7) r espectiv ely. T he expression levels of protein spots 1, 4, 5, 6 and 7 was dramatically downregulated, and that of protein spots 2 and 3 had a slightly opposite tendency on the 3rd day. The obviously decreased period is particularly interesting as it was consistent with the induction period of adventitious root primordial of tree peony plantlet in vitro. The ATP synthase β-subunit could be consumed for assembling the ATP synthase in order to supply energy to the rooting process. Therefore, we speculated that the ATP synthase β-subunit was involved in adventitious root initiation of tree peony plantlets in vitro and we expect that further studies should be carried out in order to export its action mechanism.

      • KCI등재

        Effects of Different Sucrose Concentrations in the Culture Medium on the Growth of Colored Zantedeschia In Vitro under CO2 Enrichment Conditions

        Zheng Wang,Yinglong Song,Liyun Shi,Yuzhen Guo,Wenqian Shang,Dan He,Song Lin He 한국화훼학회 2016 화훼연구 Vol.24 No.2

        Carbon source, an essential nutrient for plant growth, mainly includes exogenous sugar and CO2 of the environment in vitro. Therefore, the exogenous sugar and CO2 of the environment make the important roles in tissue culture. The aim of this study is to investigate the effects of different sugar concentrations (0, 10, 15 and 30 g·L-1) on the growth of colored Zantedeschia in vitro under certain CO2 concentration and explore the optimal sugar concentration. The plantlets in vitro of colored Zantedeschia had the largest root number, root weight, and root vigor under 0 g·L-1 (sugar-free culture) treatment. And they had the largest plant height, leaf length and leaf chlorophyll content, but p oor r oot v igor under 3 0 g·L-1 sugar. This study indicated that the optimal condition for proliferation and seedling culture of colored Zantedeschia plantlets in vitro was MS medium with 30 g·L-1 sugar, and the suitable medium for rooting culture and transplanting of colored Zantedeschia was MS medium with sugar-free culture under CO2 enrichment condition.

      • KCI등재

        Effects of Root Pruning on Adventitious Root Formation, Enzyme Activities, and Hormone Levels in Paeonia suffruticosa ‘Fengdanbai’ Seedlings

        Zheng Wang,Gege Su,Songlin He,Liyun Shi,Dan He,Wenqian Shang,Dajuan Yang 한국원예학회 2021 원예과학기술지 Vol.39 No.1

        Root pruning during early root development can optimize the root structure of woody plants. Todetermine the effects of root pruning on tree peony (Paeonia suffruticosa), seedlings of the treepeony cultivar ‘Fengdanbai’ were subjected to varying degrees or different styles of root pruning. Various indexes were analyzed, including the anatomical structure of the root, root vitality, theactivity levels of antioxidant enzymes, and the contents of endogenous hormones and their ratios. Comprehensive morphological observation showed that pruning freshly germinated roots that are 3cm long by 1/3 (i.e., removing 1 cm) yielded the greatest rooting rate (90%), lateral root initiation(2.34 per seedling), and subsequent growth. Analysis of physiological and biochemical indicatorsduring the critical rooting period (3-5 days after root pruning) showed that the levels of superoxidedismutase and abscisic acid (ABA) were decreased, while the oxidase activities of peroxidase,polyphenol oxidase, and indole acetate oxidase and the contents of the endogenous hormonesindole acetic acid (IAA), gibberellin (GA3), and zeatin (ZT) were increased. IAA plays a key role inthe process of adventitious root formation and the ratios of IAA/GA3, IAA/ABA, and IAA/ZTreflect endogenous hormone homeostasis during adventitious root formation. IAA/ABA and IAA/ZTshowed higher values in the pruned roots compared to the control roots, which favor adventitiousroot induction and elongation. These results provide a basis for the study of adventitious rootformation in tree peony.

      연관 검색어 추천

      이 검색어로 많이 본 자료