Chemokine Signaling Pathway Involved in CCL2 Expression in Patients with Rheumatoid Arthritis

Lin Zhang,Changyi Li,Min Yu,Jiayin Deng,Xing Lv,Jun Liu,Yu Xiao,Wenjie Yang,Yuru Zhang 연세대학교의과대학 2015 Yonsei medical journal Vol.56 No.4

Purpose: Rheumatoid arthritis (RA) is an inflammatory joint disorder, the progressionof which leads to the destruction of cartilage and bone. Chemokines are involvedin RA pathogenesis. In this study, we investigated the chemokine signaling pathway associated with CCL2 in peripheral blood (PB) and synovial tissues (ST) of RA patients based on our previous work about chemokine signaling pathway involvedin the activation of CCL2 production in collagen-induced arthritis rat ST. Materials and Methods: Total RNA was isolated from PB leukocytes and synoviumof the knee joint in both RA patients and control populations. Real-time polymerasechain reaction was used to determine CCL4, CCR5, c-Jun, c-Fos, and CCL2 expressions. Serum level of CCL2 was assessed by enzyme-linked immunosorbent assay, and the production of CCL2 in ST was analyzed immunohistochemically. Results: The expressions of CCL4, CCR5, c-Jun, c-Fos, and CCL2 messenger RNA in RA patients were significantly higher than those in healthy controls, both in ST and on PB leukocyte. Serum CCL2 levels were elevated in RA patients. Histological examination of rheumatoid joints revealed extensive CCL2 expression in RA ST. Conclusion: CCL2, CCL4, c-Jun, c-Fos, and CCR5 may play an important role in the recruitment of PB leukocytes into the RA joints. These data provide evidence that the chemokine signaling pathway is involved in CCL2 expression in RA patient tissues, which may contribute to chronic inflammation associated with RA. Targetingthis signaling pathway may provide a novel therapeutic avenue in RA.

Changes in plant anthocyanin levels in response to abiotic stresses: a meta-analysis

Yan Wenjie,Li Juanjuan,Lin Xinyue,Wang Lina,Yang Xiaoxiao,Xia Xiangyu,Zhang Yuxin,Yang Shaoyu,Li Hongbing,Deng Xiping,Ke Qingbo 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.5

Anthocyanins are small molecule antioxidants that play important roles in plant response and resistance to abiotic stresses. Their levels increase when plants are exposed to abiotic stress. However, the general response patterns and magnitude of anthocyanin increase, and how they confer resistance to abiotic stresses, are difficult to evaluate because of the influence of experimental variables. In this study, changes in plant anthocyanin content under different abiotic stresses and the effect of anthocyanin overproduction on various physiological indicators were investigated through meta-analysis derived from 1039 datasets of 102 studies. Results showed that among the different stress types, heavy metals, especially copper (Cu) and mercury (Hg), induced the most significant synthesis of anthocyanins. Among the different types of drought treatments, mannitol caused anthocyanin content in plants to increase by 2.4-fold, which was more significant than that of polyethylene glycol and natural drought. Furthermore, UV stress led plant anthocyanins to increase 1.3-fold in C4 plants, which was higher than that in C3 plants. In addition, anthocyanins increased the most when the applied stresses were low and moderate, and of short duration. Moreover, plants overexpressing genes encoding MYB transcription factors increased anthocyanin content by 5.8-fold and significantly improved plant stress tolerance. Among the 21 physiological indicators, glutathione and proline levels increased the most in transgenic plants overproducing anthocyanins when exposed to abiotic stress. Taken together, this meta-analysis indicates that anthocyanins enhance stress tolerance by improving the antioxidant, metal-chelating, and osmoregulatory abilities of the plant. The results presented here can help guide future applications of anthocyanins as plant growth regulators in dryland agriculture and breeding for plant stress tolerance.

A Numerical Method to Reduce the Stray Magnetic Field Around the Asymmetrical Wireless Power Transfer Coils for Electric Vehicle Charging

Zhang Wenjie,Song Jiancheng,Lin Lingyan,Ren Hui,Zheng Lijun,Gao Yunguang,Song Yuan 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.3

Wireless power transfer (WPT) is becoming increasingly popular in stationary electric vehicle charging. Unfortunately, the adverse health eff ects due to the stray magnetic fi eld created by the WPT coils have increasingly caused concern. However, the traditional stray magnetic fi eld optimization design method based on fi nite element simulation is time-consuming and resource intensive. In this paper, a numerical method to reduce the stray magnetic fi eld around the asymmetrical wireless power transfer coils is proposed while maintaining the transmission power constant. The formulas of the stray magnetic fi eld around the WPT coils are derived, and the distribution characteristics of the stray magnetic fi eld are analyzed in detail. The stray magnetic fi eld around the asymmetrical wireless power transfer coils is optimized by the numerical method, and the number of the turns for the WPT coils corresponding to the minimum stray magnetic fi eld intensity is obtained. The eff ectiveness of the numerical method is verifi ed via the fi nite element analysis tool JMAG, and it is proved that the numerical method require less computational time than the fi nite element simulation. The experiments have also been carried out to evaluate the validity of the theoretical analysis.

Nuclear Power Plant Security and Counter-Terrorist Strategy

YANG Guang,HUANG WenJie,LEI Linli 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

Nuclear power plants have long been recognized as potential targets of terrorist attacks Given the potentially catastrophic consequences of deliberate act on nuclear power plant and energy security issue followed, nuclear power plant security is elevated to a world-wide security concern. This paper focuses attention on security and vulnerability intrinsic of nuclear power plant. Firstly, the present paper summarizes the attack mode and types of terrorist, and it analyzes the defence in depth, design basis threat, target set, vulnerability from air attack, used fuel storage and risk of nuclear transports. In the end, the counter terrorist proposals are also presented and some of them are proved valid in practice in western nations, However, with the attack means constantly upgrading, more insufficiency of design being revealed, more sophisticated defensive system should be developed.

Pathogen-Imprinted Polymer Film Integrated probe/Ti₃C₂T_x MXenes Electrochemical Sensor for Highly Sensitive Determination of Listeria Monocytogenes

Xiaohua, Jiang,Zhiwen, Lv,Wenjie, Ding,Ying, Zhang,Feng, Lin The Korean Electrochemical Society 2022 Journal of electrochemical science and technology Vol.13 No.4

As one of the most hazardous and deadliest pathogens, Listeria monocytogenes (LM) posed various serious diseases to the human being, thus designing effective strategy for its detection is of great significance. In this work, by preparing Ti₃C₂T_x MXenes nanoribbon (Ti₃C₂T_xR) as carrier and selecting thionine (Th) acted simultaneously as signal probe and functional monomer, a LM pathogen-imprinted polymers (PIP) integrated probe electrochemical sensor was design to monitor LM for the first time, that was carried out through the electropolymerization of Th on the Ti₃C₂T_xR/GCE surface in the existence of LM. Upon eluting the templates from the LM imprinted cavities, the fabricated PIP/Ti₃C₂T_xR/GCE sensor can rebound LM cells effectively. By recording the peak current of Th as the response signal, it can be weakened when LM cell was re-bound to the LM imprinted cavity on PIP/Ti₃C₂T_xR/GCE, and the absolute values of peak current change increase with the increasement of LM concentrations. After optimizing three key parameters, a considerable low analytical limit (2 CFU mL^-1) and wide linearity (10-10⁸ CFU mL^-1) for LM were achieved. In addition, the experiments demonstrated that the PIP/Ti₃C₂T_xR sensor offers satisfactory selectivity, reproducibility and stability.

The supply chain model of new nuclear power plant programme

YANG Guang,HUANG WenJie,LEI Linli 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

Considering the life cycle of nuclear power plant(NPP) and high qualification required equipment, the identification of the scope on NPP supply chain, the assessment of some of the most valuable elements of supply chain and the clarification of the "pinch points" are of vital importance for ensuring the scheduled construction and sound operation of new nuclear programmes. Based on the analysis of the structure of new nuclear power build programme"s supply chain and the generalization of its elements, a supply chain model is developed. Finally, with a brief view of "pinch points" in China"s NPP industry, some counter measures for security and stabilization of the supply chains are proposed.

NON-UNIFORM EFFECT ON THE PERFORMANCE OF LITHIUM-ION POUCH BATTERY BASED ON THE ELECTRO-THERMAL MODEL

Mingbiao Chen,Fanfei Bai,Wenji Song,Shili Lin,Jie Lv,Ziping Feng 한국신재생에너지학회 2017 AFORE Vol.2017 No.11

Meta-analysis of the effect of melatonin application on abiotic stress tolerance in plants

Yang Xiaoxiao,Ren Jianhong,Li Juanjuan,Lin Xinyue,Xia Xiangyu,Yan Wenjie,Zhang Yuxin,Deng Xiping,Ke Qingbo 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.1

Melatonin is a hormone-like substance that promotes plant growth and development and alleviates stress levels. Although the physiological roles of melatonin and the underlying mechanisms have been qualitatively reviewed in plants, we do not fully understand when and how to apply melatonin to maximize its benefits. Here, we performed a meta-analysis to quantitatively evaluate the effect of melatonin on abiotic stress tolerance in plants and to determine the number of parameters modulated by melatonin. Melatonin significantly alleviated the growth inhibition induced by drought stress compared with other abiotic stresses, including salt, cold, heat, nitrogen deficit, and heavy metal toxicity, mainly owing to higher photosynthesis efficiency and antioxidant enzyme activity. Furthermore, melatonin modulated plant growth in a concentration-dependent manner and was more effective when applied to plants under moderate drought stress at an early stage via root irrigation. In addition, the impact of melatonin was greater in monocots than in dicots. Moreover, endogenous melatonin levels could be significantly increased via transgenic strategies. Among melatonin biosynthesis-related gene members, ASMT has tended to have the most influence on melatonin content in plants. In light of the rapidly developing genome editing technology, quantitatively increasing endogenous melatonin level in plant would be quite useful for moderating climatic conditions and combating desertification. Taken together, our results provide guidelines for melatonin application in crops plants for improving productivity under ongoing climate change.

Low-Intensity Shock Wave Therapy and Its Application to Erectile Dysfunction

Hongen Lei,Jing Liu,Huixi Li,Lin Wang,Yongde Xu,Wenjie Tian,Guiting Lin,Zhongcheng Xin 대한남성과학회 2013 The World Journal of Men's Health Vol.31 No.3

Although phosphodiesterase type 5 inhibitors (PDE5Is) are a revolution in the treatment of erectile dysfunction (ED) and have been marketed since 1998, they cannot restore pathological changes in the penis. Low-energy shock wave therapy (LESWT) has been developed for treating ED, and clinical studies have shown that LESWT has the potential to affect PDE5I non-responders with ED with few adverse effects. Animal studies have shown that LESWT significantly improves penile hemodynamics and restores pathological changes in the penis of diabetic ED animal models. Although the mechanisms remain to be investigated, recent studies have reported that LESWT could partially restore corpus cavernosum fibromuscular pathological changes, endothelial dysfunction, and peripheral neuropathy. LESWT could be a novel modality for treating ED, and particularly PDE5I non-responders with organic ED, in the near future. However, further extensive evidence-based basic and clinical studies are needed. This review intends to summarize the scientific background underlying the effect of LESWT on ED.

Calcium signalling mediated the regulation of growth and polysaccharide accumulation by light quality in Dendrobium offi cinale protocorms

Ting Lei,Suping Gao,Xiao Lin,Kaihui Zhang,Yingqi Liu,Wenji Li,Di Hu,Xiaofang Yu,Mingyan Jiang,Qibing Chen,Ying Sun 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.2

This study selected Dendrobium offi cinale protocorms as the experimental material and used diff erent light-quality treatmentsand calcium signalling inhibitors to explore the mechanism of the biological response of calcium to light quality. The results indicate that a 1:3 red:blue (R:B) light ratio promotes the accumulation of biomass and polysaccharides in theprotocorm. Nevertheless, this eff ect can be entirely counteracted by calmodulin (CaM) inhibitors and calcium ion-chelatingagents. The possible mechanisms of this eff ect are as follows: (1) The Ca 2+ -CaM signal-regulated photosynthetic apparatusis improved by the R:B 1:3 light, increasing the CaM content and Ca 2+ -ATPase activity. Thus, R:B 1:3 light increases thedevelopment of the photosynthetic apparatus in the D. offi cinale protocorm, enhances its photosynthetic productivity, andpromotes the activities of sucrose phosphatase; accordingly, biomass and sucrose accumulation increase. (2) R:B 1:3 lightenhances Ca 2+ -CaM signalling and regulates glycometabolic processes to promote the activities of sucrose synthase, thusincreasing fructose and glucose levels and providing suffi cient synthetic precursors to promote polysaccharide production.