Genetic variation of Vp1 in Sichuan wheat accessions and its association with pre-harvest sprouting response

Jirui Wang,Yaxi Liu,Yan Wang,Zhenhong Chen,Shuai Dai,Wenguang Cao,George Fedak,Xiujing Lan,Yuming Wei,Dengcai Liu,Youliang Zheng 한국유전학회 2011 Genes & Genomics Vol.33 No.2

Pre-harvest sprouting (PHS) in bread wheat is a major abiotic constraint reducing yield and influencing the production of high quality grain. In China both spring and winter wheat regions are affected by PHS. Sichuan lies in southwest China,where the most of rainfall occurs during April to September when wheat is harvested. The present investigation was conducted to identify the allelic variability of Vp1, a gene that plays a role in maintenance and induction of dormancy,among Sichuan landraces and recent cultivars with different dormancy levels and to find potential sources of PHS resistance for breeding. Sichuan landrace and cultivar wheat accessions had a wide range of dormancy levels. The average germination index (GI) of Sichuan landrace accessions was 0.232, whereas at 0.674 it was much higher for cultivars. The different dormancy levels between landraces and cultivars indicated that pre-harvest sprouting resistance might have been neglected in recent Sichuan wheat breeding programs. The average GI of white grained accessions was higher than for red grained accessions. Particular Vp-1B gene fragments were specific in landraces or cultivars and in white or red grained accessions. The results indicated that Vp-1B markers could be used to distinguish cultivars and landraces. Significant relationships between certain Vp-1B allelesand GI of Sichuan wheat accessions were shown by Spearman’s rank correlation analysis.

High-Voltage Transmission Line Foreign Object and Power Component Defect Detection Based on Improved YOLOv5

Wang Shanshan,Tan Weiwei,Yang Tengfei,Zeng Liang,Hou Wenguang,Zhou Quan 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.1

With the outstanding performance of deep learning in the feld of computer vision, the automatic visual detection of foreign bodies in transmission lines and electrical equipment defects by inspection robots and Unmanned Aerial Vehicles based on neural networks has become an attractive topic in smart grid. However, in practical application scenarios, small-sized target defects pose a great challenge to the detection accuracy of existing mainstream deep learning detection networks with limited perceptual felds. To solve the above problems, the paper proposes a detection model of YOLOv5 transmission line inspection image. Firstly, the key target images under diferent backgrounds and attitudes are collected and preprocessed. Specifcally, in order to improve the perception ability of networks for small-sized targets, the K-means clustering algorithm is used to optimize the size of the anchor box, which efectively improves the ftting ability of the key target features. Then, recursive gated convolution is used as the backbone network to improve the ability to extract key target features. Finally, considering the concealment of small-scale features, the space-to-depth convolution module is added to the neck network to realize down-sampling and retain all the information in the channel dimension. In addition, a feature prediction layer is added to optimize the scale of network detection, and a Simple Parameter-Free Attention Module is added to further optimize the characterization of network features. The experimental results show that the accuracy and recall of the proposed network are 96.8% and 93.3%, respectively. The average detection accuracy reaches 97.1%, which is 3.8% higher than that of YOLOv5 network, 5.2% higher than YOLOv6 and 1.0% higher than that of YOLOv7 network. The proposed method signifcantly improves the detection performance of critical targets and defects of high-voltage transmission lines.

Improving Mycoplasma ovipneumoniae culture medium by a comparative transcriptome method

Xiaohui Wang,Wenguang Zhang,Yongqing Hao 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.2

Mycoplasma ovipneumoniae (Mo) is difficult to culture, resulting in many difficulties in related research and application. Since nucleotide metabolism is a basic metabolism affects growth, this study conducted a “point-to-point” comparison of the corresponding growth phases between the Mo NM151 strain and the Mycoplasma mycoides subsp. capri (Mmc) PG3 strain. The results showed that the largest difference in nucleotide metabolism was found in the stationary phase. Nucleotide synthesis in PG3 was mostly de novo, while nucleotide synthesis in NM151 was primarily based on salvage synthesis. Compared with PG3, the missing reactions of NM151 referred to the synthesis of deoxythymine monophosphate. We proposed and validated a culture medium with added serine to fill this gap and prolong the stationary phase of NM151. This solved the problem of the fast death of Mo, which is significant for related research and application.

A new thermosensitive polymer as nonadhesive liquidembolism material

Chengru Zhao,Qin Wang,Jianwen Meng,Wenguang Liu,Zuoqin Liu 한국물리학회 2005 Current Applied Physics Vol.5 No.5

Thermosensitive copolymers poly(NIPAm-co-NNPAm) ofN-isopropylacrylamide (NIPAm) withN-n-propylacrylamide(NNPAm) were synthesized by free radical copolymerization using potassium persulfate (KPS) and sodium sulte (Na2SO3) as ini-tiator. FTIR and1H NMR conrmed the structure of the copolymer poly(NIPAm-co-NNPAm). The dynamic mechanical analysisand dynamic contact angle were determined. The lower critical solution temperature (LCST) of copolymers was investigated byDSC. The results indicated the LCST decreased with the higher NNPAm contents. The phase transition of the copolymer solutionoccurred at body temperature within 30 s and the copolymers changed into soft solids. The copolymer could be used as a new non-adhesive liquid embolism material for the intervenient therapy of the cerebral arterio-venous malformation (AVM)..

Energetics and Atomic Structures of Cu₂Te Overlayers on CdTe(111)

Choi, Jin-Ho,Zhu, Wenguang,Ho, Kai-Ming,Wang, Deliang,Zhang, Zhenyu American Chemical Society 2015 The Journal of Physical Chemistry Part C Vol.119 No.9

<P>Although Cu<SUB>2</SUB>Te is widely used as a back contact material in CdTe solar cells, the exact atomic structure of the Cu<SUB>2</SUB>Te/CdTe interface remains unclear. Using first-principles calculations within density functional theory, we search for possible structures of Cu<SUB>2</SUB>Te overlayers on CdTe(111) surfaces based on our recent theoretical determination of a stable layered structure of bulk Cu<SUB>2</SUB>Te. We discover that the unstrained bulklike configuration is energetically more favorable than various epitaxial configurations because of the significant energy costs associated with the in-plane strain, even though the first epitaxial overlayer is already more stable than bulk Cu<SUB>2</SUB>Te. Our thermodynamic analysis further confirms that the unstrained bulklike interface structure is still stable at typical experimental growth temperatures (∼700 K). It is also found that van der Waals forces play a considerable role in the stacking of the Cu<SUB>2</SUB>Te overlayers. These findings are discussed in connection with existing and new experimental results and will likely stimulate future systematic experiments.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2015/jpccck.2015.119.issue-9/jp511776e/production/images/medium/jp-2014-11776e_0003.gif'></P>

Graphene nanosheet/silicone composite with enhanced thermal conductivity and its application in heat dissipation of high-power light-emitting diodes

Hai Yan Zhang,Yingxi Lin,Danfeng Zhang,Wenguang Wang,Yuxiong Xing,Jin Lin,Haoqun Hong,Chunhui Li 한국물리학회 2016 Current Applied Physics Vol.16 No.12

Heat dissipation from light-emitting diodes (LEDs) has become a serious problem because of the LEDs' high luminosity and power. This problem could be solved by improving the dissipation efficiency of each LED system component. A microwave-reduced graphene nanosheet/silicone (GN/silicone) composite with a high thermal conductivity and stability was prepared by mechanical blending. The thermal conductivity of the composite reaches 2.7 W/(m K) with only 1.5 wt% loading, and is 12 times higher than the pure silicone matrix. When used as a thermal interface material between high-power LED chip module substrates and heat sinks, the thermal conductive GN/silicone composite could decrease the temperature difference between the substrate and shell. It could also improve the system heat transfer efficiency. The temperature gap between the heat slug and the heat sink was less than 2 C with a 1.5 wt% loading of GNs.

Identification of Nucleolin as a Lipid-Raft-Dependent Beta1-Integrin-Interacting Protein in A375 Cell Migration

Jiajia Bi,Xianlu Zeng,Ruifei Wang,Yue Zhang,Xiaoqing Han,Khamal Kwesi Ampah,Wenguang Liu 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.6

Lipid rafts are related to cell surface receptor function. Integrin is a major surface receptor protein in cell adhesion and migration on the extracellular matrix (ECM). Here, we showed that lipid rafts played a critical role in human melanoma A375 cell spreading and migration on fibronectin; an important component of the ECM that interacts with 1 integrin. We found that the disruption of lipid rafts did not markedly inhibit the expression and activation of 1 integrin. By coimmunoprecipitation and mass spectrometry, we investigated the influence of lipid rafts on the 1 integrin complex and identified nucleolin as a potential lipid-raft-dependent 1-integrin-interacting protein. Upon confirmation of the interaction between 1 integrin and nucleolin, further studies revealed that nucleolin colocalized with 1 integrin in lipid rafts and raft disruption interrupted their association. In addition, knockdown of nucle-olin markedly attenuated A375 cell spreading and migration on fibronectin. Taken together, we demonstrated that nucleolin is a critical lipid-raft-dependent 1-integrin-inte-racting protein in A375 cell spreading and migration on fibronectin.