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      • Influence of the Donor Side of Photosystem II on the Photogeneration of Superoxide Radicals and Chlorophyll a Fluorescence

        Weng, Jun,Zhang, Suping,Pan, Jingxi,Jinxing, Chen,Xu, Chunhe Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2

        Direct EPR evidence of the photo-generation of superoxide radicals ( $O_2$$^{-.}$) was obtained by using spin trapping techniques in spinach photosystem II (PSII) membranes. $O_2$$^{-.}$ was detected by following the formation of 5-diethoxyphosphoryl-5-methyl-1 -pyrroline-N-oxide (DEPMPO) superoxide adducts, DEPMPO-OOH. The significant increase of the EPR signal amplitude of DEPMPO-OOH in N$H_2O$H-, CaC $l_2$- and NaCl-treated PSII membranes showed that the oxygen-evolving system has a close relation to the $O_2$$^{-.}$ production. PSII membranes with inactivated donor side could not prevent the $O_2$$^{-.}$ production efficiently. Treatments on PSII donor side also influence the maximum level and the kinetics of Chlorophyll (Chi) a fluorescence. Results suggested that manganese cluster and extrinsic proteins might affect Chi a fluorescence in ways different from that happens at the acceptor side of PSII.SII.SII.

      • KCI등재

        Characterization of the B cell receptor repertoire of patients with acute coronary syndrome

        Weng Ruiqiang,Liu Sudong,Gu Xiaodong,Zhong Zhixiong 한국유전학회 2022 Genes & Genomics Vol.44 No.1

        Background: Acute coronary syndrome (ACS) is a complex cardiovascular disease whose development involves the dysregulation of adaptive immune responses. Though it has been proven that T cells associate with inflammation in the development of ACS, the function of B cells in disease remains unclear. Objective: The aim of this study was to reveal the diversity of the B cell receptor (BCR) repertoire of patients with ACS. Methods: We conducted a pilot study to sequence the immune repertoire of peripheral blood mononuclear cells (PBMCs) from patients with ACS, including acute myocardial infarction (AMI) and unstable angina (UA), and quantitatively characterized BCR repertoires by bioinformatics analysis. Results: We found that patients with AMI and UA had lower BCR repertoire diversity compared with controls with normal coronary arteries (NCA). Lower percentages of productive unique BCR nt sequences and higher percentages of top 200 unique BCR sequences were identified in AMI and UA patients than NCA controls. Patients had various preferential usage of V and J genes from B cell clones in accordance with the disease severity of coronary arteries. AMI patients had distinct CDR3 amino acids, and their frequency differed among patients with ACS. Conclusions: Our results indicate that differential BCR signatures represent an imprint of distinct repertoires among ACS patients. This study thereby opens up the prospect of studying disease-relevant B cells to better understand and treat ACS.

      • KCI등재

        Dietary supplementation with different types of fiber in gestation and lactation: effects on sow serum biochemical values and performance

        Weng Ruey-Chee 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.8

        Objective: Three types of dietary fiber were fed to sows during gestation and lactation stages to monitor their physiological and metabolic adaptations during the pre-partum period and to determine how these effects may influence the lactation period and sow performance. Methods: Soon after breeding, 54 sows were selected and were fed with 20% supplementation as fed of wheat bran (WB), soya hulls (SH), or rice hulls (RH) in diets during gestation and lactation. Sows were weighed, backfat thickness was measured ultrasonically and jugular blood samples were collected from all sows. The litter size was equalized to 10, by fostering piglets from sows on the same treatment. Results: Sows gained 22.0, 21.8, and 25.5 kg of net maternal body weight during gestation (for WB, SH, and RH sows, respectively; p = 0.007). There was no treatment effect on the body weight change during lactation (p = 0.158), however RH sows consumed an average of 133.66 kg of feed, WB sows took 121.29 kg and SH sows took 126.77 kg during lactation (p<0.001). The SH litters gained an average of 59.34 kg of weight during lactation, while other litters gained 51.58 and 49.98 kg (for WB and RH litters, respectively; p<0.001). Exception for aspartate aminotransferase and alanine aminotransferase, measured serum biochemical values were broadly in agreement with earlier reports. Despite the use of additional vegetable oil to balance the energy level, RH sows still had lower concentrations of serum triglycerides in late gestation. Conclusion: Different types of fibrous ingredients in the gestation diet influenced most of the investigated reference values for sows. The values of serum biochemical parameters were generally not affected by fiber type during the lactation stage. The SH supplementation for sows is an effective approach to give heavier litters at birth and weaning and to increase voluntary feed intake in early lactation.

      • SCISCIESCOPUS
      • KCI등재

        Evaluation of cadmium ratio for conceptual design of a cyclotronbased thermal neutron radiography system

        Weng-Sheng Kuo 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.7

        An approximate method for calculating the cadmium ratio of a cyclotron-based thermal neutron radiography system was developed. In this method, the Monte-Carlo code, MCNP6.2, was employed to calculate the neutron capture rates of Au-197, and the cadmium ratio was obtained by computing the ratio of neutron capture rates. From the simulation results, the computed cadmium ratio is reasonably acceptable, and the assumption of ignoring the fast neutron contribution to the cadmium ratio is valid.

      • KCI등재

        Therapeutic Effects of Fibroblast Growth Factor-21 on Diabetic Nephropathy and the Possible Mechanism in Type 1 Diabetes Mellitus Mice

        Weng Wenya,Ge Tingwen,Wang Yi,He Lulu,Liu Tinghao,Wang Wanning,Zheng Zongyu,Yu Lechu,Zhang Chi,Lu Xuemian 대한당뇨병학회 2020 Diabetes and Metabolism Journal Vol.44 No.4

        Background: Fibroblast growth factor 21 (FGF21) has been only reported to prevent type 1 diabetic nephropathy (DN) in the streptozotocin-induced type 1 diabetes mellitus (T1DM) mouse model. However, the FVB (Cg)-Tg (Cryaa-Tag, Ins2-CALM1) 26OVE/PneJ (OVE26) transgenic mouse is a widely recommended mouse model to recapture the most important features of T1DM nephropathy that often occurs in diabetic patients. In addition, most previous studies focused on exploring the preventive effect of FGF21 on the development of DN. However, in clinic, development of therapeutic strategy has much more realistic value compared with preventive strategy since the onset time of DN is difficult to be accurately predicted. Therefore, in the present study OVE26 mice were used to investigate the potential therapeutic effects of FGF21 on DN. Methods: Four-month-old female OVE26 mice were intraperitoneally treated with recombinant FGF21 at a dose of 100 μg/kg/day for 3 months. The diabetic and non-diabetic control mice were treated with phosphate-buffered saline at the same volume. Renal functions, pathological changes, inflammation, apoptosis, oxidative stress and fibrosis were examined in mice of all groups. Results: The results showed that severe renal dysfunction, morphological changes, inflammation, apoptosis, and fibrosis were observed in OVE26 mice. However, all the renal abnormalities above in OVE26 mice were significantly attenuated by 3-month FGF21 treatment associated with improvement of renal adenosine 5´-monophosphate (AMP)-activated protein kinase (AMPK) activity and sirtuin 1 (SIRT1) expression. Conclusion: Therefore, this study demonstrated that FGF21 might exert therapeutic effects on DN through AMPK-SIRT1 pathway.

      • SCIESCOPUSKCI등재

        Effect of Individual, Group or ESF Housing in Pregnancy and Individual or Group Housing in Lactation on Sow Behavior

        Weng, R.C.,Edwards, S.A.,Hsia, L.C. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.11

        To evaluate the effect of different housing systems on sow behavior, 80 gilts were randomly allocated at puberty to four treatments: i) sow stall in gestation followed by farrowing crate (SC), ii) group housing with individual feeding in gestation followed by farrowing crate (GC), iii) ESF (Electronic Sow Feeding) system in gestation followed by farrowing crate (EC), and iv) ESF system followed by group farrowing pen (EG). Behavioral observations were carried out on a total of 16 animals per treatment at the following stages: first day of allocation to housing treatment, day of service, 80 days after service, 109 days after service on entry to farrowing accommodation, 24 h before farrowing, day of farrowing, 14, 27 and 28 days after farrowing, at weaning. On each occasion, individual animals were observed for a 24 period with one minute time sampling. There were significant differences (p<0.001) between stages of the reproductive cycle for all the behavior patterns in all treatments. On the first day in experimental housing treatments, sows spent more time rooting and dog-sitting. Activity and investigatory behavior decreased as pregnancy progressed. An activity peak was apparent just before farrowing, followed by a high level of inactivity on the day of farrowing. Time spent active, eating and drinking increased as lactation progressed, and greatest activity and locomotion was seen immediately following weaning. There were significant differences between housing treatments (p<0.01) for standing, moving, eating, drinking, dog-sitting and lying. During pregnancy SC sows spent more time standing, rooting, drinking and dog sitting, while EC sows spent less time rooting and drinking and more time lying. During lactation, GC sows spent more time standing, moving and eating, less time dog sitting and lateral lying. Nursing frequency was reduced in GC sows (p<0.001). The maternal and piglet behaviors were influenced strongly by environment during lactation. However, it was also shown that previous housing history can influence the maternal behavior in the pre-farrowing stage and during early lactation.

      • A Systematic Review of MRI, Scintigraphy, FDG-PET and PET/CT for Diagnosis of Multiple Myeloma Related Bone Disease - Which is Best?

        Weng, Wan-Wen,Dong, Meng-Jie,Zhang, Jun,Yang, Jun,Xu, Qin,Zhu, Yang-Jun,Liu, Ning-Hu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

        Aim: The purpose of the current study was to conduct a systematic review of the published literature to evaluate the diagnostic accuracy of FDG-PET, PTE/CT, MRI and scintigraphy for multiple myeloma related bone disease. Methods: Through a search of PubMed, EMBASE, and the Cochrane Library, two reviewers independently assessed the methodological quality of each study. We estimated pooled sensitivity, specificity, positive and negative likelihood ratios (PLR and NLR), and two sample Z-tests were conducted to evaluate for differences in sensitivity, specificity, area under the curve (AUC), and the $Q^*$ index between any two diagnostic modalities. Results: A total of 17 studies were reviewed. The MRI had a pooled sensitivity of 0.88, specificity of 0.68, AUC of 0.897, and $Q^*$ index of 0.828, whereas for MIBI, the corresponding values were 0.98, 0.90, 0.991, and 0.962, respectively, and for bone scan, they were 066, 0.83, 0.805, and 0.740, respectively. The corresponding values of MIBI were 0.98, 0.90, 0.991, and 0.962, respectively. For PET and PET/CT, the values were 0.91, 0.69, 0.927 and 0.861, respectively. Statistically significant differences were not found in the sensitivity, specificity, AUC, and $Q^*$ index between MRI, scintigraphy, FDG-PET and PET/CT. Conclusions: On the condition that X ray is taken as a reference in our study, we suggested that FDG-PET, PTE/CT, MRI and scintigraphy are all associated with high detection rate of bone disease in patients with MM. Thus, in clinical practice, it is recommended that we could choose these tests according to the condition of the patient.

      • A Service Pipelining Model for Web GIS Based on Distributed Environment

        Weng Yu,Cheng WenYi 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.6

        Pipeline is one of the classic technologies to enhance efficiency. When the amount of data is becoming more and more big when processing, it becomes a big problem that how to improve the efficiency with existing resources. In the meantime, a large number of older hardware and software feel difficult to deal with new task in new environment with the accumulation of time. In order to ameliorate efficiency of web GIS with low cost, this paper propose a service pipelining model for Web GIS based on distributed environment. The system build a basic service library to reuse plenty of existing resource, and design a segment system to organize pipeline in level of service to improve efficiency. The model can be used in many platforms and occasions, not limited to the application we proposed in this paper. The model we proposed has been proved work well in our experiments and test.

      • SSCISCIESCOPUS

        Mobile taxi booking application service’s continuance usage intention by users

        Weng, Gooi Sai,Zailani, Suhaiza,Iranmanesh, Mohammad,Hyun, Sunghyup Sean Pergamon 2017 Transportation Research Part D. Transport & Enviro Vol.57 No.-

        <P><B>Abstract</B></P> <P>The long-term development of a mobile booking taxi application service depends on the continued use of its passengers. The aim of this study is to investigate the determinants of the mobile taxi booking application service’s continuance intention, using the technology continuance theory by including the perceived risk and subjective norms. The data were collected by surveying 387 users of the mobile taxi application service. The data were analysed by applying the partial least squares technique. The analysis showed that the technology continuance theory has extensive power to explain the continuance intention to use the mobile booking taxi application. Subjective norms also have a significant effect on the attitude of mobile booking taxi application users which represents an important contribution to technology continuance theory extension. The theoretical and practical significances of the study have been discussed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The early acceptance of an MTB Apps does not guarantee continuation. </LI> <LI> Studies on continuance intention of users toward MTB App use are still lacking. </LI> <LI> Results will help App developers to grasp the factors that result in continued MTB App use. </LI> </UL> </P>

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