A Novel Missense Mutation of Keratin 17 Gene in a Chinese Family with Steatocystoma Multiplex

( Wei Wei Ha ),( Jing Wang ),( Wen Wang ),( Hong Yang Fu ),( Hua Yang Tang ),( Xian Fa Tang ),( Jun Zhu ),( Xian Yong Yin ),( Sen Yang ),( Xue Jun Zhang ) 대한피부과학회 2013 Annals of Dermatology Vol.25 No.4

Paraoxonase 1 (PON1) Q192R Gene Polymorphism and Cancer Risk: A Meta-Analysis Based on 30 Publications

Zhang, Meng,Xiong, Hu,Fang, Lu,Lu, Wei,Wu, Xun,Huang, Zhan-Sen,Wang, Yong-Qiang,Cai, Zhi-Ming,Wu, Song Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10

Common genetic variation Q192R in the paraoxonase 1 (PON1) gene has been considered to be implicated in the development of many cancers. Nevertheless, results from the related studies were inconsistent. To elucidate the association, we performed a meta-analysis for 8,112 cases and 10,037 controls from 32 published case-control studies. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of the association by STATA 12.0 software. Overall, we revealed that the PON1-192R allele was associated with a reduced risk of the overall cancers. Moreover, in the stratified analysis by cancer types (breast cancer, prostate cancer, brain cancer etc.), the results showed that PON1-192R allele was associated with a decreased risk in breast cancer (R vs Q: OR=0.605, 95% CI=0.378-0.967, $P_{heterogeneity}=0.000$; RR vs QQ: OR=0.494, 95% CI=0.275-0.888, $P_{heterogeneity}=0.002$; RQ vs QQ: OR=0.465, 95% CI=0.259-0.835, $P_{heterogeneity}=0.000$; and RR+RQ vs QQ: OR=0.485, 95% CI=0.274-0.857, $P_{heterogeneity}=0.000$), and associated with prostate cancer in homozygote (RR vs QQ: OR=0.475, 95% CI=0.251-0.897, $P_{heterogeneity}=0.001$) and recessive models (RR vs RQ+QQ: OR=0.379, 95% CI=0.169-0.853, $P_{heterogeneity}=0.000$), while an increased risk was identified in lymphoma (R vs Q: OR=1.537, 95% CI=1.246-1.896, $P_{heterogeneity}=0.944$; RR vs QQ: OR=2.987, 95% CI=1.861-4.795, $P_{heterogeneity}=0.350$; RR+RQ vs QQ: OR=1.354, 95% CI=1.021-1.796, $P_{heterogeneity}=0.824$; and RR vs RQ+QQ: OR=2.934, 95% CI=1.869-4.605, $P_{heterogeneity}=0.433$), and an increased risk in prostate cancer under heterozygote comparison (RQ vs QQ: OR=1.782, 95% CI=1.077-2.950, $P_{heterogeneity}=0.000$) and dominant models (RR+RQ vs QQ: OR=1.281, 95% CI=1.044-1.573, $P_{heterogeneity}=0.056$). When subgroup analysis that performed by the control source (hospital based or population based), a decreased risk of the overall cancers was revealed by homozygote (RR vs QQ: OR=0.601, 95% CI=0.366-0.987, $P_{heterogeneity}=0.000$) and dominant models (RR vs RQ+QQ: OR= 0.611, 95% CI=0.384-0.973, $P_{heterogeneity}=0.000$) in hospital based group. Stratifying by ethnicity, a significantly reduced risk of the overall cancers under allele contrast model (R vs Q: OR=0.788, 95% CI=0.626-0.993, $P_{heterogeneity}=0.000$) was uncovered in Caucasian. In summary, these findings suggested that PON1 Q192R polymorphism was associated with a reduced risk of the overall cancers, nevertheless, it might increase cancer susceptibility of prostate and lymphoma risk. Large well-designed epidemiological studies will be continued on this issue of interest.

The exchange bias effect and Griffiths phase in La1.5Sr0.5Co1-Ni MnO6: The impact of the divalent Ni

Zhang Hongguang,Wang Sen,Chen Wei,Wang Mingjun,Li Yongtao,Xie Liang 한국물리학회 2023 Current Applied Physics Vol.53 No.-

The effects of Co-site doping of Ni2+ ions on the crystal structure, electronic structure, and magnetic properties of La1.5Sr0.5CoMnO6 are investigated. The doping causes an increase in the anti-site disorder and a change in the content of Co2+/3+ and Mn3+/4+, as well as a change in the spin state of Co ions. Ni doping introduces Ni2+-O2-- Mn4+ ferromagnetic interactions to increase the ferromagnetic transition temperature. Samples display different dominant clusters, and field cooling promotes the presence of ferromagnetic clusters, allowing an increase in the Griffiths phase. Interestingly, Ni doping enables tuning the conventional and spontaneous exchange bias (EB) effects, increasing the EB field for intermediate concentrations. Moreover, the maxima of both EB fields do not occur in the same sample, which leads to a new understanding of the relationship between these two effects. The possible mechanisms related to the uncompensated spin and competition between ferromagnetic and antiferromagnetic clusters are discussed, respectively.

Ginsenoside Rg3 nanoparticles with permeation enhancing based chitosan derivatives were encapsulated with doxorubicin by thermosensitive hydrogel and anti-cancer evaluation of peritumoral hydrogel injection combined with PD-L1 antibody

Wu Hao,Wei Guoli,Luo Lixia,Li Lingchang,Gao Yibo,Tan Xiaobin,Wang Sen,Chang Haoxiao,Liu Yuxi,Wei Yingjie,Song Jie,Zhang Zhenhai,Huo Jiege 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Combination of chemotherapy and immune checkpoint inhibitor therapy has greatly improved the anticancer effect on multiple malignancies. However, the efficiency on triple-negative breast cancer (TNBC) is limited, since most patients bear “cold” tumors with low tumor immunogenicity. Doxorubicin (DOX), one of the most effective chemotherapy agents, can induce immunogenic cell death (ICD) and thus initiating immune response.In this study, to maximize the ICD effect induced by DOX, chitosan and cell-penetrating peptide (R6F3)-modified nanoparticles (PNPs) loaded with ginsenoside Rg3 (Rg3) were fabricated using the self-assembly technique, followed by co-encapsulation with DOX based on thermo-sensitive hydrogel. Orthotopic tumor model and contralateral tumor model were established to observe the antitumor efficacy of the thermo-sensitive hydrogel combined with anti-PD-L1 immunotherapy, besides, the biocompatibility was also evaluated by histopathological.Rg3-PNPs strengthened the immunogenic cell death (ICD) effect induced by DOX. Moreover, the hydrogel co-loading Rg3-PNPs and DOX provoked stronger immune response in originally nonimmunogenic 4T1 tumors than DOX monotherapy. Following combination with PD-L1 blocking, substantial antitumor effect was achieved due to the recruitment of memory T cells and the decline of adaptive PD-L1 enrichment.The hydrogel encapsulating DOX and highly permeable Rg3-PNPs provided an efficient strategy for remodeling immunosuppressive tumor microenvironment and converting immune “cold” 4T1 into “hot” tumors.

LETTER TO THE EDITOR : One Mutation of the ED1 Gene in a Chinese Han Family with X-Linked Hypohidrotic Ectodermal Dysplasia

( Jing Wang ),( Wei Wei Ha ),( Wen Wang ),( Hua Yang Tang ),( Xian Fa Tang ),( Xian Dong Zheng ),( Jun Zhu ),( Xian Yong Yin ),( Sen Yang ),( Xue Jun Zhang ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.1

Analysis of Small Fragment Deletions of the APC gene in Chinese Patients with Familial Adenomatous Polyposis, a Precancerous Condition

Chen, Qing-Wei,Zhang, Xiao-Mei,Zhou, Jian-Nong,Zhou, Xin,Ma, Guo-Jian,Zhu, Ming,Zhang, Yuan-Ying,Yu, Jun,Feng, Ji-Feng,Chen, Sen-Qing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.12

Background: : Familial adenomatous polyposis (FAP) is an autosomal dominant inherited disease mainly caused by mutations of the adenomatous polyposis coli (APC) gene with almost complete penetrance. These colorectal polyps are precancerous lesions that will inevitable develop into colorectal cancer at the median age of 40-year old if total proctocolectomy is not performed. So identification of APC germline mutations has great implications for genetic counseling and management of FAP patients. In this study, we screened APC germline mutations in Chinese FAP patients, in order to find novel mutations and the APC gene germline mutation characteristics of Chinese FAP patients. Materials and Methods: The FAP patients were diagnosed by clinical manifestations, family histories, endoscope and biopsy. Then patients peripheral blood samples were collected, afterwards, genomic DNA was extracted. The mutation analysis of the APC gene was conducted by direct polymerase chain reaction (PCR) sequencing for micromutations and multiplex ligation-dependent probe amplification (MLPA) for large duplications and/or deletions. Results: We found 6 micromutations out of 14 FAP pedigrees, while there were no large duplications and/or deletions found. These germline mutations are c.5432C>T(p. Ser1811Leu), two c.3926_3930delAAAAG (p.Glu1309AspfsX4), c.3921_3924delAAAA (p.Ile1307MetfsX13), c3184_3187delCAAA(p.Gln1061AspfsX59) and c4127_4126delAT (p.Tyr1376LysfsX9), respectively, and all deletion mutations resulted in a premature stop codon. At the same time, we found c.3921_3924delAAAA and two c.3926_3930delAAAAG are located in AAAAG short tandem repeats, c3184_3187delCAAA is located in the CAAA interrupted direct repeats, and c4127_4128 del AT is located in the 5'-CCTGAACA-3', 3'-ACAAGTCC-5 palindromes (inverted repeats) of the APC gene. Furthermore, deletion mutations are mostly located at condon 1309. Conclusions: Though there were no novel mutations found as the pathogenic gene of FAP in this study, we found nucleotide sequence containing short tandem repeats and palindromes (inverted repeats), especially the 5 bp base deletion at codon 1309, are mutations in high incidence area in APC gene,.

Triptolide Inhibits Histone Methyltransferase EZH2 and Modulates the Expression of Its Target Genes in Prostate Cancer Cells

Tamgue, Ousman,Chai, Cheng-Sen,Hao, Lin,Zambe, John-Clotaire Daguia,Huang, Wei-Wei,Zhang, Bin,Lei, Ming,Wei, Yan-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

The histone methyltransferase EZH2 (enhancer of zeste homolog 2) plays critical roles in prostate cancer (PCa) development and is a potential target for PCa treatment. Triptolide possesses anti-tumor activity, but it is unknown whether its therapeutic effect relates with EZH2 in PCa. Here we described EZH2 as a target for Triptolide in PCa cells. Our data showed that Triptolide suppressed PCa cell growth and reduced the expression of EZH2. Overexpression of EZH2 attenuated the Triptolide induced cell growth inhibition. Moreover, Triptolide treatment of PC-3 cells resulted in elevated mRNA levels of target genes (ADRB2, CDH1, CDKN2A and DAB2IP) negatively regulated by EZH2 as well as reduced mRNA levelsan of EZH2 positively regulated gene (cyclin D1). Our findings suggest the PCa cell growth inhibition mediated by Triptolide might be associated with downregulation of EZH2 expression and the subsequent modulation of target genes.

Association of Measures of Glucose Metabolism with Colorectal Cancer Risk in Older Chinese: A 13-Year Follow-up of the Guangzhou Biobank Cohort Study-Cardiovascular Disease Substudy and Meta-Analysis

Shu Yi Wang,Wei Sen Zhang,Chao Qiang Jiang,Ya Li Jin,Tong Zhu,Feng Zhu,Lin Xu 대한당뇨병학회 2024 Diabetes and Metabolism Journal Vol.48 No.1

Background: Abnormal glucose metabolism is a risk factor for colorectal cancer (CRC). However, association of glycosylated hemoglobin (HbA1c) with CRC risk remains under-reported. We examined the association between glycemic indicators (HbA1c, fasting plasma glucose, fasting insulin, 2-hour glucose, 2-hour insulin, and homeostasis model of risk assessment-insulin resistance index) and CRC risk using prospective analysis and meta-analysis.Methods: Participants (<i>n</i>=1,915) from the Guangzhou Biobank Cohort Study-Cardiovascular Disease Substudy were included. CRC events were identified through record linkage. Cox regression was used to assess the associations of glycemic indicators with CRC risk. A meta-analysis was performed to investigate the association between HbA1c and CRC risk.Results: During an average of 12.9 years follow-up (standard deviation, 2.8), 42 incident CRC cases occurred. After adjusting for potential confounders, the hazard ratio (95% confidence interval [CI]) of CRC for per % increment in HbA1c was 1.28 (95% CI, 1.01 to 1.63) in overall population, 1.51 (95% CI, 1.13 to 2.02) in women and 1.06 (95% CI, 0.68 to 1.68) in men. No significant association of other measures of glycemic indicators and baseline diabetes with CRC risk was found. Meta-analyses of 523,857 participants including our results showed that per % increment of HbA1c was associated with 13% higher risk of CRC, with the pooled risk ratio being 1.13 (95% CI, 1.01 to 1.27). Subgroupanalyses found stronger associations in women, colon cancer, Asians, and case-control studies.Conclusion: Higher HbA1c was a significant predictor of CRC in the general population. Our findings shed light on the pathology of glucose metabolism and CRC, which warrants more in-depth investigation.

Nanofiber Induced Silk Fibroin Nanofiber/Silk Fibroin (SFNF/SF) Fibrous Scaffolds for 3D Cell Culture

Shiyang Chen,Tongda Lei,Yunrui Zhang,Huancheng Wu,Sen He,Wei Liu,Jie Fan,Yong Liu 한국섬유공학회 2023 Fibers and polymers Vol.24 No.2

Fibrous 3D scaffold with small fiber diameter has the similar topographic and structural characteristics of native extracellularmatrix (ECM), which provides the beneficial microenvironment for cell adhesion, growth, migration, proliferation. However,the pore structure of the biopolymer scaffold is crucial for cell regulation and tissue regeneration in practical application. Inthis report, we proposed a nanofiber induced silk fibroin nanofibers/silk fibroin (SFNF/SF) fibrous scaffold with homogeneousmicron pores using fast freeze-drying technology under -196 °C. The physical, chemical and biological performance of thescaffold was investigated. Ethanol post treatment of the scaffold led to the conformation transition of silk fibroin from randomcoil (silk I) to beta-sheet (silk II) and increase of the crystallinity of the scaffold, which greatly improved the stability of thescaffold in water. Scaffolds made from 2 to 6% SFNF/SF solution with SFNF/SF ratio ranging from 1:1 to 1:8 exhibited threedimensional (3D) fibrous structure with porosity of 80–85% and pore size ranging from 5 to 15 μm due to the entanglementof the nanofibers. And the fibrous structure of the scaffolds can be adjusted by controlling the concentration of the SFNF/SF solution and the SFNF/SF ratio. Cell culture suggested that the 3D fibrous network structure with micron pores showedadvantages for cell migration comparing with the lamella structure scaffold. After 7 day’s culture, cells migrated to about240 μm inside the 6% 1:1 scaffold, while only about 160 μm inside the 6% 1:16 scaffold. The nanofiber induced micro porousSFNF/SF scaffolds by fast freeze-drying technology is potential for preparation of micron porous scaffold.

Terpenoid composition and the anticancer activity of Acanthopanax trifoliatus

Dong-Li Li,Xi Zheng,Yu-Chan Chen,Sen Jiang,Wei-Min Zhang,Huaqian Wang,Zhi-Yun Du,Kun Zhang 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.1

The petroleum ether and ethyl acetate fractions of extract from an edible and medicinal plant Acanthopanax trifoliatus were found to show significant inhibitory effects against SF-268, MCF-7, HepG2 and NCIH460 cancer cells. Two new ursane-type triterpenoids, acantrifoic acid C (1) and acantrifoic acid D (2), along with five known triterpenoids (3–7) and eight known diterpenoids (8–15) were obtained from these two fractions. To the best of our knowledge, this is the first report concerning the isolation of compounds (5–12, 14, 15) from A. trifoliatus. Among all the isolated compounds, 3, 5 and 8 from the ethyl acetate fraction showed the strongest inhibitory effects against cancer cells, while 12 and 13 from the petroleum ether fraction showed moderate activities. These terpenoid compounds may be responsible for the anticancer activities of A. trifoliatus. Our study provides the first evidence that terpenoids from A. trifoliatus exert anticancer activities and indicates that A. trifoliatus may be a useful edible plant for further development of anticancer health supplement.