Thiazinogeldanamycin, a New Geldanamycin Derivative Produced by Streptomyces hygroscopicus 17997

( Si Yang Ni ),( Lin Zhuan Wu ),( Hong Yuan Wang ),( Mao Luo Gan ),( Yu Cheng Wang ),( Wei Qing He ),( Yi Guang Wang ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.6

A new geldanamycin (GDM) derivative was discovered and isolated from the fermentation broth of Streptomyces hygroscopicus 17997. Its chemical structure was elucidated as thiazinogeldanamycin by LC-MS, sulfur analysis, and NMR. The addition of cysteine to the fermentation medium significantly stimulated the production level of thiazinogeldanamycin, suggesting cysteine as a precursor of thiazinogeldanamycin production. Although showing a decreased cytotoxicity against HepG2 cancer cells, thiazinogeldanamycin exhibited an improved water solubility and photostability. Thiazinogeldanamycin may represent the first natural GDM derivative characterized so far that uses GDM as its precursor. Its appearance also clearly indicates that an appropriate end-point of fermentation is of critical importance for the maximal production of GDM by Streptomyces hygroscopicus 17997.

Constructing ZnCo2O4@CTP microspheres as a high-energy lithium storage material

Yu-Rui Ji,Si-Yu Qi,Jian-Cang Wang,Pengfei Wang,Ning Ren,Ting-Feng Yi 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-

Transition metal oxides are deemed as a promising substitute for graphite anode materials of Li-ion batteries(LIBs) on account of their large specific capacity. Nonetheless, the large volume change in the processof charging and discharging leads to low capacity retention. Herein, a well-designed ZnCo2O4@coaltar pitch based porous carbon (ZCO-CTP) microsphere is prepared to construct the high-performanceanode of LIBs. The coating modification of CTP significantly reduces the polarization and charge transferresistance and improves the migration ability of ions and electrons, thus achieving a superior reversiblecapacity and cycling stability of ZCO-CTP anode. The battery with obtained ZCO-CTP5 provides a largecharge capacity of 406.17 mAh/g at 1000 mA g1 compared with pristine ZCO (only 157.78 mAh/g). Even after 1000 cycles at 1000 mA g1, the battery can achieve a charge/discharge capacity of598.46/601.17 mAh/g. We believe that the application of CTP will provide a new perspective for theexploitation of high-performance anode materials for LIBs.

TAZ as a novel regulator of oxidative damage in decidualization via Nrf2/ARE/Foxo1 pathway

Yu Hai-Fan,Zheng Lian-Wen,Yang Zhan-Qing,Wang Yu-Si,Wang Ting-Ting,Yue Zhan-Peng,Guo Bin 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

TAZ, as a crucial effector of Hippo pathway, is required for spermatogenesis and fertilization, but little is known regarding its physiological function in uterine decidualization. In this study, we showed that TAZ was localized in the decidua, where it promoted stromal cell proliferation followed by accelerated G1/S phase transition via Ccnd3 and Cdk4 and induced the expression or activity of stromal differentiation markers Prl8a2, Prl3c1 and ALP, indicating the importance of TAZ in decidualization. Knockdown of TAZ impeded HB-EGF induction of stromal cell proliferation and differentiation. Under oxidative stress, TAZ protected stromal differentiation against oxidative damage by reducing intracellular ROS and enhancing cellular antioxidant capacity dependent on the Nrf2/ARE/Foxo1 pathway. TAZ strengthened the transcriptional activity of Nrf2 which directly bound to the antioxidant response element (ARE) of Foxo1 promoter region. Additionally, silencing TAZ caused accumulation of intracellular ROS through heightening NOX activity whose blockade by APO reversed the disruption in stromal differentiation. Further analysis revealed that TAZ might restore mitochondrial function, as indicated by the increase in ATP level, mtDNA copy number and mitochondrial membrane potential with the reduction in mitochondrial superoxide. Additionally, TAZ modulated the activities of mitochondrial respiratory chain complexes I and III whose suppression by ROT and AA resulted in the inability of TAZ to defend against oxidative damage to stromal differentiation. Moreover, TAZ prevented stromal cell apoptosis by upregulating Bcl2 expression and inhibiting Casp3 activity and Bax expression. In summary, TAZ might mediate HB-EGF function in uterine decidualization through Ccnd3 and ameliorate oxidative damage to stromal cell differentiation via Nrf2/ARE/Foxo1 pathway.

월드컵 평화의공원에 대한 유니버설디자인 적용성 평가와 이용자 만족도 연구

왕사우(Wang, Si Yu),김동찬(Kim, Dong Chan),민병욱(Min, Byoung Wook) 한국디자인문화학회 2017 한국디자인문화학회지 Vol.23 No.1

현재 많은 도시공원 내 공간과 시설이 예전의 기준에 맞춰 조성되어 이용자의 질적 요구수준에 부응하지 못하는 문제들이 드러나고 있다. 이에 따라 다양한 이용자들을 배려함과 동시에 보편성이 있는 유니버설디자인 적용이 필요한 시점으로 생각한다. 이에 본 연구는 서울시의 대표적인 도시공원인 월드컵 평화의공원에 대한 유니버설디자인 적용수준을 파악하고, 물리적 환경에 대한 이용자 만족도를 조사⋅분석하였다. 광범위한 문헌연구를 통해 유니버설디자인의 개념과 원리를 고찰하고 관련법규와 선행연구를 바탕으로 9개의 평가항목 그룹으로 구성된 평가의 틀을 도출하여 이를 토대로 월드컵 평화의공원에서 현장평가를 실시하였으며, 평가도구의 개별항목 중에서 이용자가 인지 할 수 있는 요소들을 7개의 그룹으로 추출 및 요약하여 설문을 구성하고 물리적 환경에 대한 이용자들의 만족도 평가를 시행하였다. 실측 평가의 결과는 월드컵 공원 유니버설디자인 적용정도가 보통수준으로 나타났으나(75.6/100), 이용자의 만족도는 전반적으로 양호한 것(3.49/5.0)으로 나타났다. 이상의 결과를 바탕으로 구체적인 구간별 주요 문제점을 도출하여 개선방향을 제시하였다. 모든 사람들이 이용함에 불편함이 없도록 이용자들의 환경에 대한 만족도를 높이기 위해서는 유니버설디자인 관점에서의 시설 정비 및 개선이 필요하며, 본 연구의 연구결과는 월드컵 평화의공원 향후의 관리⋅정비 그리고 도시공원설계 시 기초자료로 활용될 수 있을 것이다. The spaces and facilities in a number of current urban parks have been very outdated and irrelevant to /meet the level of the users’ demands. Regarding that aging society is one of the most pressing concerns of our society, it is important to consider the diverse needs and, particularly, the application of the universal design approach. This paper investigates the level of universal design applied in the World Cup Peace Park in Seoul, and analyzes the user satisfaction. The concepts and the principles of the universal design are redefined based on extensive literature review, and the evaluation framework of nine criteria is formulated. Then, an onsite evaluation is carried out at the World Cup Peace Park. Among the seven groups of individual items of the evaluation tools, the elements that can be recognized by the users are extracted for the survey questionnaire was organized. An evaluation of the user satisfaction was carried out. Through the actual measurement, several problems of the World Cup Peace Park were found. Therefore, the extent of the applications of the universal designs showed to be the ordinary level. In the evaluation of the user satisfaction, it showed to be satisfactory overall. Regarding this, the improvement direction was presented. With regard to the use of the park, in order to raise the level of satisfaction regarding the environment of the users so that all the people do not have any inconveniences, the facility maintenance and the improvement are required in the viewpoint of the universal designs. And such results can be utilized as the basic materials when managing and maintaining the World Cup Peace Park in the future and when planning city parks.

한국어 ‘N1+의+N2’형 단어에 대한 고찰

왕사우(Wang Si yu)(王思宇) 형태론 2016 형태론 Vol.18 No.2

현대 한국어에서 ‘의’는 관형격 표지의 역할을 하고 있다. 하지만 흥미로운 것은 ‘의’가 단어 내부에 나타나기도 한다는 것인데, 본고는 이러한 점에 주목하여 한국어의 ‘N1+의+N2’형 단어들에 대해 검토하였다. 이들은 기존 연구에서 주목하지 않았던 것으로 구 형식을 가지면서도 단어의 구실을 한다. 따라서 본고는 이들 ‘N1+의+N2’형 단어의 정체성을 밝히고 분류를 하는 것을 목적으로 하였다. 이를 위하여 먼저 ‘N1+의+N2’형 단어의 존재 양상을 살펴보았다. 그 다음은 이들 단어를 자질로 분석하는 방법을 도입하여 구 형식을 띠면서 단어의 기능을 하는 ‘N1+의+N2’형 구성의 단어성을 검토하였다. 이에 따라 ‘스승의 날’처럼 띄어쓰기를 하는 ‘N1+의+N2’형 단어의 단어 자격을 인증하고 기존의 ‘전문용어’와 ‘관용 표현’에 해당하는 ‘N1+의+N2’형 단어에 대해 ‘준단어’란 범주를 설정할 수 있음을 주장하였다. 마지막으로는 ‘N1+의+N2’형 단어의 분류 방법을 제시하였다. 더욱이 자질을 분석하여 단어성을 확인하는 방법은 한국어의 모든 단어 심지어 다른 언어에서도 사용될 가능성이 있어 본고의 의의를 확인할 수 있다. In modern Korean, ui is regarded as the genitive case marker. However, what is interesting is that it also appears inside the word. In this paper, the words of ‘N1+ui+N2’ form were studied. This kind of words, which have both the form of phrase and the features of word, have not been noted in previous research. So the purpose of this paper is to confirm the features of this kind of words, and then classify them into subclasses. Firstly the existing aspects of them were described. Then the method of analyzing features was used to check the wordhood of the phrasal words of ‘N1+ui+N2’ form. Accordingly the words of ‘N1+ui+N2’ form such as seu-seung-ui nal(Teachers day) which do the word spacing were qualified within the word category. It was claimed to set up a new category called semi-word for the existing terminologies and idiomatic expressions of ‘N1+ui+N2’ form. At last, the classification method of this form of word was presented. Moreover, the method proposed in this paper of analyzing the features for confirming the wordhood may also be used in all the words in Korean even in other languages. In other words, it may possibly be universally applicable.

Network Analysis of microRNAs, Genes and their Regulation in Mantle Cell Lymphoma

Deng, Si-Yu,Guo, Xiao-Xin,Wang, Ning,Wang, Kun-Hao,Wang, Shang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

The pathogenesis of mantle cell lymphoma, a special subtype of lymphoma that is invasive and indolent and has a median survival of 3 to 4 years, is still partially unexplained. Much research about genes and miRNAs has been conducted in recent years, but interactions and regulatory relations of genetic elements which may play a vital role in genesis of MCL have attracted only limited attention. The present study concentrated on regulatory relations about genes and miRNAs contributing to MCL pathogenesis. Numerous experimentally validated raw data were organized into three topology networks, comprising differentially expressed, associated and global examples. Comparison of similarities and dissimilarities of the three regulating networks, paired with the analysis of the interactions between pairs of elements in every network, revealed that the differentially expressed network illuminated the carcinogenicity mechanism of MCL and the related network further described the regulatory relations involved, including prevention, diagnosis, development and therapy. Three kinds of regulatory relations for host genes including miRNAs, miRNAs targeting genes and genes regulating miRNAs were concluded macroscopically. Regulation of the differentially expressed miRNAs was also analyzed, in terms of abnormal gene expression affecting the MCL pathogenesis. Special regulatory relations were uncovered. For example, auto-regulatory loops were found in the three topology networks, key pathways of the nodes being highlighted. The present study focused on a novel point of view revealing important influencing factors for MCL pathogenesis.

Association of Six Susceptibility Loci with Prostate Cancer in Northern Chinese Men

Zhang, Yu-Rong,Xu, Yong,Yang, Kuo,Liu, Ming,Wei, Dong,Zhang, Yao-Guang,Shi, Xiao-Hong,Wang, Jian-Ye,Yang, Fan,Wang, Xin,Liang, Si-Ying,Zhao, Cheng-Xiao,Wang, Fei,Chen, Xin,Sun, Liang,Zhu, Xiao-Quan,Zh Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Background/Aim: Six prostate cancer (PCa) susceptibility loci were identified in a genome-wide association study (GWAS) in populations of European decent. However, the associations of these 6 single-nucleotide polymorphisms (SNPs) with PCa has remained tobe clarified in men in Northern China. This study aimed to explore the loci associated with PCa risk in a Northern Chinese population. Methods: Blood samples and clinical information of 289 PCa patients and 288 controls from Beijing and Tianjin were collected. All risk SNPs were genotyped using polymerase chain reaction (PCR)-high resolution melting curve technology and gene sequencing. Associations between PCa and clinical covariates (age at diagnosis, prostate-specific antigen [PSA], Gleason score, tumor stage, and level of aggressiveness) and frequencies of alleles and genotypes of these SNPs were analyzed using genetic statistics. Results: Among the candidate SNPs, 11p15 (rs7127900, A) was associated with PCa risk (P = 0.02, odds ratio [OR] = 1.64, 95% confidence interval [CI] = 1.09-2.46). Genotypes showed differences between cases and controls on 11p15 (rs7127900, A), 11q13 (rs7931342, T), and HNF1B (rs4430796, A) (P = 0.03, P = 0.01, and P = 0.04, respectively). The genotype TG on 11q13 (rs7931342, T) was positively associated with an increased Gleason score (P = 0.04, OR = 2.15, 95% CI = 1.02-4.55). Patients carrying TG on 17q24 (rs1859962, G) were negatively associated with an increased body mass index (BMI) (P = 0.03, OR = 0.44, 95% CI = 0.21-0.92) while those with AG on HNF1B (rs4430796, A) were more likely to have PSA increase (P = 0.002). Conclusion: Our study suggests that 11p15 (rs7127900, A) could be a susceptibility locus associated with PCa in Northern Chinese. Genotype TG on 11q13 (rs7931342, T) could be related to an increased Gleason score, AG on HNF1B (rs4430796, A) could be associated with PSA increase, and TG on 17q24 (rs1859962, G) could be negatively associated with an increased BMI in Chinese men with PCa.

한국어 ‘X+에(ㅅ)+X’형(型) 명사(名詞)에 대한 연구(硏究)

왕사우 ( Wang Si-yu ) 한국어문교육연구회 2018 어문연구(語文硏究) Vol.46 No.1

本稿는 한국어에서 助詞가 單語 形成에 참여하는 현상으로서 ‘X+에 (ㅅ)+X’型 名詞를 살폈다. ‘X+에(ㅅ)+X’형 명사들의 형성 과정을 중요시하여 合成語나 派生語로 분류하기보다는 별도의 분류를 설정해야 할 필요성을 제기하였다. 이를 위해 먼저 한국어 ‘X+에(ㅅ)+X’형 명사의 樣相을 ‘X+에+X’형과 ‘X+엣+X’형으로 나눠서 차례로 검토하였다. 그리고 ‘X+에(ㅅ)+X’型 構成의 변천 과정과 ‘X+에(ㅅ)+X’型 單語의 形成을 논하였다. 이들은 주로 근대 한국어 시기에 通時的 單語化나 共時的 單語化에 의해 만들어진 것이다. 마지막으로는 分析보다 形成의 관점에서 ‘X+에(ㅅ)+X’형 명사의 단어 체계 내의 위치에 대해 논의를 하였다. 본고에서는 ‘X+에(ㅅ)+X’형 명사를 統辭的 結合語로 귀속시켰지만 ‘에’로 끝나는 助詞 結合語와 구분하기 위해 助詞 結合語를 세분화해야 할 필요성을 제기하였다. 이와 같이 조사가 단어 형성에 참여하는 현상은 한국어의 膠着語로서의 특성을 잘 보여준다는 점에서 주목할 필요가 있다. In this paper, the nouns of ‘X+ey(s)+X’ form are studied. It is a phenomenon in Korean that Josa participates in the word formation process. The process of word formation is concerned, so this kind of words is not regarded as the compound word or derived word in this study. Firstly, nouns of ‘X+ey(s)+X’ form were devided into ‘X+ey+X’ form and ‘X+eys+X’ form and the appearances of them were reviewed in order. Nextly, this kind of words’ transition and formation process were studied. The words are mainly made in the Modern Korean period by the diachronic wordification or synchronic wordification. Finally, from the viewpoint of formation rather than analysis, the location of nouns of ‘X+ey(s)+X’ form in the word system was discussed. These nouns of ‘X+ey(s)+X’ form were attributed to the Syntactically Combined Words. However, it was claimed that the Jasa Combined Words need to be subdivided in purpose to distinguish the words of ‘X+ey(s)+X’ form from the words which end with Josa ‘ey’. The phenomenon that Jasa participates in the formation reveals the characteristics of Korean as an agglutinative language.

Study on Degradation Mechanism of Carbon Fiber-reinforced Anhydride-cured Resin-based Matrix Composites by a Benzyl Alcohol/NaOH System

Zhong-yu Si,Zhong-xiao Sun,Lianwei Ye,Ai-jun Gao,Yu Wang 한국섬유공학회 2022 Fibers and polymers Vol.23 No.11

In recent years, with the increasing demand for carbon fiber-reinforced polymer (CFRP), the recycling of its wastehas become a serious problem that restricts the wider application of CFRP. This study used a benzyl alcohol/NaOH system atatmospheric pressure to degrade the CFRP of epoxy-resin matrix cured by anhydride, and the influence of NaOH dosage ondegradation rate was investigated. The degradation mechanism of the resin was analyzed using Fourier transform infraredspectroscopy, gas chromatography-mass spectrometry and Electro-Spray Ionization-Mass Spectroscopy. The structure andproperties of the recycled carbon fibers were characterized using scanning electron microscopy, X-ray photoelectronspectroscopy and a monofilament tensile strength test. The experimental results indicated that the benzyloxy nucleophilicgroup produced by benzyl alcohol in alkaline conditions selectively attacked the cross-linking ester bond of the resin andbroke the c ross-linking point. Finally, the resin was degraded by removing the curing agent. When the m ass ratio of NaOH toCFRP (w(NaOH):w(CFRP)) was not under 1:1 and the reaction conditions were 190 °C, 1 h and atmospheric pressure, thedegradation rate of the resin reached 98.4 %. The degradation products of the resin were insoluble in benzyl alcohol andprecipitated, which was conducive to the recovery and reuse of the benzyl alcohol. The surface oxygen content of therecycled carbon fiber was similar to that of the original carbon fiber, and the tensile strength of recycled carbon fibermonofilament remained above 95 % of the original.

Discovery of novel glycoside hydrolases from C-glycoside-degrading bacteria using sequence similarity network analysis

Wei Bin,Wang Ya-Kun,Yu Jin-Biao,Wang Si-Jia,Yu Yan-Lei,Xu Xue-Wei,Wang Hong 한국미생물학회 2021 The journal of microbiology Vol.59 No.10

C-Glycosides are an important type of natural product with significant bioactivities, and the C-glycosidic bonds of C-glycosides can be cleaved by several intestinal bacteria, as exemplified by the human faeces-derived puerarin-degrading bacterium Dorea strain PUE. However, glycoside hydrolases in these bacteria, which may be involved in the C-glycosidic bond cleavage of C-glycosides, remain largely unknown. In this study, the genomes of the closest phylogenetic neighbours of five puerarin-degrading intestinal bacteria (including Dorea strain PUE) were retrieved, and the protein-coding genes in the genomes were subjected to sequence similarity network (SSN) analysis. Only four clusters of genes were annotated as glycoside hydrolases and observed in the genome of D. longicatena DSM 13814T (the closest phylogenetic neighbour of Dorea strain PUE); therefore, genes from D. longicatena DSM 13814T belonging to these clusters were selected to overexpress recombinant proteins (CG1, CG2, CG3, and CG4) in Escherichia coli BL21(DE3). In vitro assays indicated that CG4 efficiently cleaved the O-glycosidic bond of daidzin and showed moderate β-D-glucosidase and β-D-xylosidase activity. CG2 showed weak activity in hydrolyzing daidzin and pNP- β-D-fucopyranoside, while CG3 was identified as a highly selective and efficient α-glycosidase. Interestingly, CG3 and CG4 could be selectively inhibited by daidzein, explaining their different performance in kinetic studies. Molecular docking studies predicted the molecular determinants of CG2, CG3, and CG4 in substrate selectivity and inhibition propensity. The present study identified three novel and distinctive glycoside hydrolases, highlighting the potential of SSN in the discovery of novel enzymes from genomic data.